BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are clos...BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are closely associated with vaginal diseases.AIM To explore the effects of GBS status and vaginal cleanliness on vaginal microecosystems.METHODS We collected 160 vaginal swabs from pregnant women and divided them into the following four groups based on GBS status and vaginal cleanliness:GBS-positive+vaginal cleanliness I–II degree,GBS-negative+vaginal cleanliness I–II degree,GBS-positive+vaginal cleanliness III–IV degree,and GBS-negative+vaginal cleanliness III–IV degree.Samples were subjected to 16S rRNA gene amplicon sequencing.RESULTS Alpha diversity analysis showed that the Shannon index did not significantly differ between the four groups.We identified significant variation in taxa abundance between the GBS-positive and GBS-negative groups and between the vaginal cleanliness I–II degree and III–IV degree groups.Principal coordinate analysis and non-metric multidimensional scaling analysis further confirmed the microbial diversity of the four groups.Moreover,the linear discriminant analysis demonstrated that Lactobacillus jensenii and Actinobacteria were strongly associated with GBS-positive status,and Lactobacillus iners,Lactobacillaceae,Lactobacillus,Lactobacillales,Bacilli and Firmicutes were closely correlated with GBS-negative status.CONCLUSION GBS status and vaginal cleanliness significantly affect vaginal microbiome differences in pregnant women.Our findings provide instructional information for clinical antibiotic treatment in pregnant women with different GBS statuses and vaginal cleanliness degrees.展开更多
目的探讨正常足月顺产新生儿脐静脉血pH值能否取代脐动脉血pH值来评估新生儿健康状况,及其影响因素。方法选取2020年1—12月符合纳入标准的150例产妇及新生儿为研究对象,用GEM Premier 3000进行血气分析,采用配对t检验,进行线性相关分...目的探讨正常足月顺产新生儿脐静脉血pH值能否取代脐动脉血pH值来评估新生儿健康状况,及其影响因素。方法选取2020年1—12月符合纳入标准的150例产妇及新生儿为研究对象,用GEM Premier 3000进行血气分析,采用配对t检验,进行线性相关分析及回归分析。结果脐带动、静脉血平均pH值分别为(7.278±0.073)和(7.352±0.061),二者差异有统计学意义(P<0.001);脐带动、静血pH值在统计学上高度相关(r=0.782,P<0.05);脐带动、静脉血pH值、第二产程时长、产次、新生儿性别和需要吸氧分娩差异有统计学意义(P<0.05)。结论脐带动、静脉血pH值有显著差异,但是二者也显著相关;对于正常足月顺产新生儿,可以采集脐静脉血代替脐动脉血进行血气分析。展开更多
文摘BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are closely associated with vaginal diseases.AIM To explore the effects of GBS status and vaginal cleanliness on vaginal microecosystems.METHODS We collected 160 vaginal swabs from pregnant women and divided them into the following four groups based on GBS status and vaginal cleanliness:GBS-positive+vaginal cleanliness I–II degree,GBS-negative+vaginal cleanliness I–II degree,GBS-positive+vaginal cleanliness III–IV degree,and GBS-negative+vaginal cleanliness III–IV degree.Samples were subjected to 16S rRNA gene amplicon sequencing.RESULTS Alpha diversity analysis showed that the Shannon index did not significantly differ between the four groups.We identified significant variation in taxa abundance between the GBS-positive and GBS-negative groups and between the vaginal cleanliness I–II degree and III–IV degree groups.Principal coordinate analysis and non-metric multidimensional scaling analysis further confirmed the microbial diversity of the four groups.Moreover,the linear discriminant analysis demonstrated that Lactobacillus jensenii and Actinobacteria were strongly associated with GBS-positive status,and Lactobacillus iners,Lactobacillaceae,Lactobacillus,Lactobacillales,Bacilli and Firmicutes were closely correlated with GBS-negative status.CONCLUSION GBS status and vaginal cleanliness significantly affect vaginal microbiome differences in pregnant women.Our findings provide instructional information for clinical antibiotic treatment in pregnant women with different GBS statuses and vaginal cleanliness degrees.
文摘目的探讨正常足月顺产新生儿脐静脉血pH值能否取代脐动脉血pH值来评估新生儿健康状况,及其影响因素。方法选取2020年1—12月符合纳入标准的150例产妇及新生儿为研究对象,用GEM Premier 3000进行血气分析,采用配对t检验,进行线性相关分析及回归分析。结果脐带动、静脉血平均pH值分别为(7.278±0.073)和(7.352±0.061),二者差异有统计学意义(P<0.001);脐带动、静血pH值在统计学上高度相关(r=0.782,P<0.05);脐带动、静脉血pH值、第二产程时长、产次、新生儿性别和需要吸氧分娩差异有统计学意义(P<0.05)。结论脐带动、静脉血pH值有显著差异,但是二者也显著相关;对于正常足月顺产新生儿,可以采集脐静脉血代替脐动脉血进行血气分析。
