The carbon-13 nuclear magnetic resonance spectra of seventeen protoilludane ses- quiterpenoid aromatic esters from the artificially cultured mycelium of Armillaria mellea (Vahl.ex Fr.) Quel.have been analysed and assi...The carbon-13 nuclear magnetic resonance spectra of seventeen protoilludane ses- quiterpenoid aromatic esters from the artificially cultured mycelium of Armillaria mellea (Vahl.ex Fr.) Quel.have been analysed and assigned.All the ^(11)CNMR signals of the model compounds melleolide 5 and compounds 8,10,11,13,14,15,and 16 can be assigned on the basis of the multiplicity of the sig- nals in the off-resonance decoupled spectra or INEPT spectra.^(11)C-~1H COSY and long-range ^(13)C-~1H COSY.The assignments of the ^(13)CNMR spectra of other protoilludane sesquiterpenoid aromatic esters were completed by comparison with the model compounds as well as the electronic effects of substi- tuted group on the molecules.The present results indicate that ^(11)CNMR spectroscopy provides an ef- fective method for characterizing the protoilludane sesquiterpenoid aromatic esters.展开更多
A new protoilludane sesquiterpenoid aromatic ester,named armillarizin (1)has been isolated from the artificially cultured mycelium of Armillaria me- llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on...A new protoilludane sesquiterpenoid aromatic ester,named armillarizin (1)has been isolated from the artificially cultured mycelium of Armillaria me- llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on the ba- sis of spectral analyses.The relative configuration was established from the nuclear Overhauser effects and from the values of ~1H-~1H coupling constants.展开更多
Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Ir...Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Iran.Methods:The essential oils of S.lavandulifolia Vahl from seven locations were obtained by hydro-distillation and analysed by gas chromatography and gas chromatography-mass spectrometry.Results:The results revealed that distinct differences in the content of compounds depending on region of sample collection.The main constituents of the essential oils wereα-thujone(0.3%-32.3%),α-pinene(trace to 37.3%),myrcene(0.5%-15.9%),β-phellandrene(1.1%-37.9%),germacrene D(0.4%-11.3%),△-cadinene(trace to 11.6%)and 1,4-methano-1 H-indene(trace to 10.1%).Conclusions:The results of the present study indicated that essential oil components of S.lavandulifolia Vahl can be varied with genetic(ecotype),environmental conditions and geographic origin,In general,the essential oils of various populations of S.lavandulifolia Vahl were rich in monoterpenoids and sesquiterpenoids.展开更多
Objective: To investigate the proximate and mineral composition of the Piper retrofractum(P. retrofractum) vahl. Fruit and to evaluate its total alkaloids, phenol and flavonoid. Methods: The proximate composition of P...Objective: To investigate the proximate and mineral composition of the Piper retrofractum(P. retrofractum) vahl. Fruit and to evaluate its total alkaloids, phenol and flavonoid. Methods: The proximate composition of P. retrofractum fruit was ananlyzed using standard protocols according to Indonesian Standard and Association of Official Analytical Chemist. Meanwhile, mineral composition of the fruit was analyzed using inductively coupled plasma-mass spectrometry. Phytochemical screening and quantification were performed using standard protocols according to Harborn and spectrophotometric methods. Results: The results showed that P. retrofractum fruit contained carbohydrate(63.4%), crude protein(11.4%), total ash(4.29%), dietary fiber(28.8%) and total fat(2.97%). The fruit also contained calcium, copper, iron, magnesium, phosphor, potassium, sodium and zinc in different concentrations. Additionally, quinone, sterol, glycosides and alkaloid were detected in both n-hexane and ethyl acetate extracts. Moreover, tannin was presented also in ethylacetate and methanol extracts. Meanwhile, methanol extract contained sterol, glycosides, flavones, tannin and alkaloid. The results also revealed that methanol extract of the fruit contained highest phenol compared to other extract. Finally, small quantity of flavonoid(0.060 0%±0.000 2%) was observed. Conclusions: The overall results show that P. retrofractum contains potential nutritional and phytochemicals values, which support their function for pharmaceutical purposes.展开更多
Three new compounds, suspenolic acid (1), forsythenside A (2), and forsythenside B (3) have been isolated from the fruits of Forsythia suspensa. Their structures were elucidated by spectral methods including IR, H-1- ...Three new compounds, suspenolic acid (1), forsythenside A (2), and forsythenside B (3) have been isolated from the fruits of Forsythia suspensa. Their structures were elucidated by spectral methods including IR, H-1- and C-13-NMR, DEPT, H-1-H-1 COSY, H-1-C-13 COSY, NOE difference, HMBC, FABMS, CIMS, and chemical reactions.展开更多
The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method invol...The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method involved the S. lavandulifolia Vahl extract acting as a reducing and stabilizing agent. The nanoparticles were characterized by transmission electron microscopy, dynamic light scattering analysis and UV-vis absorption and Fourier transform-infrared spectroscopies. Stability under physiological conditions is important for medical applications. The stability of the nanoparticles was compared with that of conventional citrate-capped nanoparticles, under both synthetic and physiological conditions. The nanoparticles synthesized from the S. lavandulifolia Vahl extract were stable under physiological conditions, in contrast with conventional citrate-capped nanoparticles.展开更多
Horizontal attenuation total reflection-Fourier transform infrared spectroscopy （HATR-FTIR） is used to measure the FTIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. ...Horizontal attenuation total reflection-Fourier transform infrared spectroscopy （HATR-FTIR） is used to measure the FTIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. In order to extrude the difference between Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed, continuous wavelet transform （CWT） is used to decompose the FFIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. Three main scales are selected as the feature extracting space in the CWT domain. According to the distribution of FTIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed, three feature regions are determined at every spectra band at selected three scales in the CWT domain. Thus nine feature parameters form the feature vector. The feature vector is input to the radial basis function neural network （RBFNN） to train so as to accurately classify the Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. 110 couples of FI＇IR are used to train and test the proposed method, where 60 couples are used as training samples and 50 couples are used as testing samples. Experimental results show that the accurate recognition rate between Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed is respectively 96% and 98% by using the proposed method.展开更多
文摘The carbon-13 nuclear magnetic resonance spectra of seventeen protoilludane ses- quiterpenoid aromatic esters from the artificially cultured mycelium of Armillaria mellea (Vahl.ex Fr.) Quel.have been analysed and assigned.All the ^(11)CNMR signals of the model compounds melleolide 5 and compounds 8,10,11,13,14,15,and 16 can be assigned on the basis of the multiplicity of the sig- nals in the off-resonance decoupled spectra or INEPT spectra.^(11)C-~1H COSY and long-range ^(13)C-~1H COSY.The assignments of the ^(13)CNMR spectra of other protoilludane sesquiterpenoid aromatic esters were completed by comparison with the model compounds as well as the electronic effects of substi- tuted group on the molecules.The present results indicate that ^(11)CNMR spectroscopy provides an ef- fective method for characterizing the protoilludane sesquiterpenoid aromatic esters.
文摘A new protoilludane sesquiterpenoid aromatic ester,named armillarizin (1)has been isolated from the artificially cultured mycelium of Armillaria me- llea(Vahl.ex Fr)Quel.(Tricholomataceae).Its structure was deduced on the ba- sis of spectral analyses.The relative configuration was established from the nuclear Overhauser effects and from the values of ~1H-~1H coupling constants.
基金Supported by deputy of Researches and Technology.Islamic Azad University of Shahrckord Branch.Iran(Grant no.IAUSHK:6121)
文摘Objective:To examine the chemical variability in inflorescences of wild populations of Stachys lavandulifolia Vahl(S.lavandulifolia)collected throughout two provinces(lsfahan and Chaharmahal va Bakhtiary),Southwest Iran.Methods:The essential oils of S.lavandulifolia Vahl from seven locations were obtained by hydro-distillation and analysed by gas chromatography and gas chromatography-mass spectrometry.Results:The results revealed that distinct differences in the content of compounds depending on region of sample collection.The main constituents of the essential oils wereα-thujone(0.3%-32.3%),α-pinene(trace to 37.3%),myrcene(0.5%-15.9%),β-phellandrene(1.1%-37.9%),germacrene D(0.4%-11.3%),△-cadinene(trace to 11.6%)and 1,4-methano-1 H-indene(trace to 10.1%).Conclusions:The results of the present study indicated that essential oil components of S.lavandulifolia Vahl can be varied with genetic(ecotype),environmental conditions and geographic origin,In general,the essential oils of various populations of S.lavandulifolia Vahl were rich in monoterpenoids and sesquiterpenoids.
基金supported by Directorate General of Resources for Science,Technology and Higher Education,Ministry of Research,Technology and Higher Education of the Republic of Indonesia(grant No.653/PKS/ITS/2017)
文摘Objective: To investigate the proximate and mineral composition of the Piper retrofractum(P. retrofractum) vahl. Fruit and to evaluate its total alkaloids, phenol and flavonoid. Methods: The proximate composition of P. retrofractum fruit was ananlyzed using standard protocols according to Indonesian Standard and Association of Official Analytical Chemist. Meanwhile, mineral composition of the fruit was analyzed using inductively coupled plasma-mass spectrometry. Phytochemical screening and quantification were performed using standard protocols according to Harborn and spectrophotometric methods. Results: The results showed that P. retrofractum fruit contained carbohydrate(63.4%), crude protein(11.4%), total ash(4.29%), dietary fiber(28.8%) and total fat(2.97%). The fruit also contained calcium, copper, iron, magnesium, phosphor, potassium, sodium and zinc in different concentrations. Additionally, quinone, sterol, glycosides and alkaloid were detected in both n-hexane and ethyl acetate extracts. Moreover, tannin was presented also in ethylacetate and methanol extracts. Meanwhile, methanol extract contained sterol, glycosides, flavones, tannin and alkaloid. The results also revealed that methanol extract of the fruit contained highest phenol compared to other extract. Finally, small quantity of flavonoid(0.060 0%±0.000 2%) was observed. Conclusions: The overall results show that P. retrofractum contains potential nutritional and phytochemicals values, which support their function for pharmaceutical purposes.
文摘Three new compounds, suspenolic acid (1), forsythenside A (2), and forsythenside B (3) have been isolated from the fruits of Forsythia suspensa. Their structures were elucidated by spectral methods including IR, H-1- and C-13-NMR, DEPT, H-1-H-1 COSY, H-1-C-13 COSY, NOE difference, HMBC, FABMS, CIMS, and chemical reactions.
文摘The synthesis of nontoxic stable gold nanoparticles is important for medical applications. An aqueous extract of the plant Stachys lavandulifolia Vahl was used to synthesize gold nanoparticles. This green method involved the S. lavandulifolia Vahl extract acting as a reducing and stabilizing agent. The nanoparticles were characterized by transmission electron microscopy, dynamic light scattering analysis and UV-vis absorption and Fourier transform-infrared spectroscopies. Stability under physiological conditions is important for medical applications. The stability of the nanoparticles was compared with that of conventional citrate-capped nanoparticles, under both synthetic and physiological conditions. The nanoparticles synthesized from the S. lavandulifolia Vahl extract were stable under physiological conditions, in contrast with conventional citrate-capped nanoparticles.
文摘Horizontal attenuation total reflection-Fourier transform infrared spectroscopy （HATR-FTIR） is used to measure the FTIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. In order to extrude the difference between Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed, continuous wavelet transform （CWT） is used to decompose the FFIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. Three main scales are selected as the feature extracting space in the CWT domain. According to the distribution of FTIR of Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed, three feature regions are determined at every spectra band at selected three scales in the CWT domain. Thus nine feature parameters form the feature vector. The feature vector is input to the radial basis function neural network （RBFNN） to train so as to accurately classify the Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed. 110 couples of FI＇IR are used to train and test the proposed method, where 60 couples are used as training samples and 50 couples are used as testing samples. Experimental results show that the accurate recognition rate between Fimbristylis miliacea （L.） Vahl seed and Fimbristhlis stauntonii Debeaux et Franch. seed is respectively 96% and 98% by using the proposed method.
