Valerian (Valeriana officinalis) extract inhibits TNF-α and iNOS gene expression in mouse LPS-activated microglial cells

Background:Inflammation and damage to neurons and other cells in the nervous system can cause disorders of the central nervous system.Microglial cells are activated by pathogen infection and injury to release nitric oxide.Valerian(Valeriana officinalis)has been used as a sedative for the treatment of neurological diseases.This study evaluated inflammation of microglial cells and tumor necrosis factorαand induced nitric oxide synthetase gene expression influenced by valerian extract.Methods:Microglial cells were isolated from mice.Lipopolysaccharide(1 ng/mL)was used to induce inflammation and nitric oxide production in cells for an hour.The inflamed cells were then treated with different concentrations(0.1,0.5,2.5,20,and 50μL/mL)of valerian alcoholic extract for 1 and 24 h.nitric oxide production and tumor necrosis factorαand induced nitric oxide synthetase gene expression were determine by Griess assay and real-time polymerase chain reaction,respectively.Results:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed no toxicity in several concentrations of the valerian extract.In addition,concentrations from 0.1 to 2.5μL/mL significantly reduced inflammation and nitric oxide production in mouse microglial cells to levels observed in control samples.Furthermore,tumor necrosis factorαand induced nitric oxide synthetase gene expression decreased when 2.5μL/mL extract was used.Conclusion:Based on these results,it can be concluded that 2.5μL/mL valerian alcoholic extract is effective as a candidate alternative medicine for reducing inflammation and nitric oxide production and consequently,the inflammatory symptoms of neurodegeneration.

SESQUITERPENES AND MONOTERPENOID WITH ACETYLCHOLINESTERASE(ACHE) INHIBITORY ACTIVITIY FROM VALERIANA OFFICINALIS VAR.LATIOFOLIA

Four new sesquiterpenoids,volvalerenal H-J(1-3)and Volvalerenic Acid K(4),along with a new monoterpenoid named Densispicnins C(5),were isolated from the ethanol extract of the root of Valeriana officinalis var.latiofolia.The structures of new compounds were determined on the basis of ID and 2D NMR,and HRESIMS.And the isolations

不同年份缬草精油挥发性成分的差异分析

利用气相色谱-飞行时间质谱法分析2020、2021年缬草(Valeriana officinalis L.)精油的挥发性物质,用峰面积归一化法计算各成分的相对含量,研究2种年份缬草精油挥发性成分的组成特征。结果表明,2种年份缬草精油中共鉴定出70种主要挥发性成分,帖烯类化合物的相对含量最高。2种年份缬草精油的挥发性成分存在明显差异,2021年缬草精油的挥发性成分种类比2020年多。在共有挥发性成分中,2-蒎烯、莰烯、β-蒎烯、柠檬烯、乙酸冰片酯、(1S)-6,6-二甲基二环[3.1.1]庚-2-烯-2-基甲醇乙酸酯、甲氧基乙酸、β-乙酸松油酯相对含量较高。

缬草精油对夏黑葡萄保鲜作用的研究

以夏黑葡萄为试验材料,研究了不同体积分数缬草(Valeriana officinalis L.)精油对夏黑葡萄常温保鲜效果中风味品质(可滴定酸含量、可溶性固形物)、货架品质(好果率、呼吸强度、失重率)及质构品质(硬度)的影响。结果表明,缬草精油处理能显著延缓夏黑葡萄中可滴定酸含量的降低速度,提高好果率,保持可滴定酸含量与可溶性固形物含量,延长果实保鲜期,其中以0.50%的缬草精油处理效果最好。

GC-MS法在黔产宽叶缬草质控中的应用

宽叶缬草(Valeriana offtcinalis L. var.latifolia Miq.)系败酱科植物.主产贵州,我国从东北至华北也有分布.具有镇静、镇痛、降压、解痉等作用.目前,对其挥发油研究较多,但采用气相色谱-质谱对药材质量进行研究较少.……

宽叶缬草种子繁殖技术研究

采集湖北省咸丰县、湖北省利川市、湖北省恩施州、贵州省和四川省5个地方宽叶缬草(Valeriana officinalis)的种子,对浸种时间、浸种水温、激素处理和冷藏处理4种萌发前的处理方式进行研究,以期得出种子萌发最佳处理方式及不同采集地种子萌发的差异,且对不同地区种子的出苗率进行了研究。结果表明,5个地区所采集的宽叶缬草种子在萌发情况上差异不显著,得出种子萌发最佳处理方式为:种子播种前-5℃冷藏28 d,冷藏完成后,用50℃、0.08%的赤霉素溶液浸泡16 h,其发芽率达到30%以上;在得出的种子最佳处理条件下,恩施州4个地区(海拔580~1436 m)在种子萌发的基础上出苗率均为40%以上。宽叶缬草种子在合适的处理条件下萌发情况较好,可在恩施州广泛推广。