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DNA-binding studies of valrubicin as a chemotherapy drug using spectroscopy and electrochemical techniques 被引量:2
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作者 Reza Hajian Parvin Hossaini +2 位作者 Zahra Mehrayin Pei Meng Woi Nafiseh Shams 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2017年第3期176-180,共5页
In this study, the molecular interactions between valrubicin, an anticancer drug, and fish sperm DNA have been studied in phosphate buffer solution(pH 7.4) using UV–Vis spectrophotometry and cyclic voltammetry techni... In this study, the molecular interactions between valrubicin, an anticancer drug, and fish sperm DNA have been studied in phosphate buffer solution(pH 7.4) using UV–Vis spectrophotometry and cyclic voltammetry techniques. Valrubicin intercalated into double stranded DNA under a weak displacement reaction with methylene blue(MB) molecule in a competitive reaction. The binding constant(kb) of valrubicin-DNA was determined as 1.75×10~3L/mol by spectrophotometric titration. The value of non-electrostatic binding constant(k_t^0) was almost constant at different ionic strengths while the ratio of k_t^0/k_b increased from 4.51% to 23.77%.These results indicate that valrubicin binds to ds-DNA via electrostatic and intercalation modes.Thermodynamic parameters including ΔH^0, ΔS^0and ΔG^0for valrubicin-DNA interaction were determined as-25.21×10~3k J/mol, 1.55×10~2k J/mol K and-22.03 k J/mol, respectively. Cyclic voltammetry study shows a pair of redox peaks for valrubicin at 0.45 V and 0.36 V(vs. Ag/Ag Cl). The peak currents decreased and peak positions shifted to positive direction in the presence of DNA, showing intercalation mechanism due to the variation in formal potential. 展开更多
关键词 valrubicin-dna interactions SPECTROSCOPY Cyclic VOLTAMMETRY
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