To track the rapidly changing temperature profiles of thermal cycling of polymerase chain reaction （PCR） accurately, an innovative feedforward variable structural proportional-integral-derivative （FVSPID） controll...To track the rapidly changing temperature profiles of thermal cycling of polymerase chain reaction （PCR） accurately, an innovative feedforward variable structural proportional-integral-derivative （FVSPID） controller was developed. Based on the step response test data of the heat block, a reduced first order model was estabfished at different operating points. Based on the reduced model, the FVSPID controller combined a feedforward path with the variable structural proportional-integral-derivative （PID） control. The modified feedforward action provided directly the optimal predictive power for the desired setpoint to speed up the dynamic response. To cooperate with the feedforward action, a variable structural PID was applied, where the P mode was used in the case of the largest errors to speed up response, whereas the PD mode was used in the case of larger errors to suppress overshoot, and finally the PID mode was applied for small error conditions to eliminate the steady state offset. Experimental results illustrated that compared to the conventional PID controller, the FVSPID controller can not only reduce the time taken to complete a standard PCR protocol, but also improve the accuracy of gene amplification.展开更多
This study investigates the structure and propagation of intraseasonal sea surface temperature（SST） variability in the South China Sea（SCS） on the 30-60-day timescale during boreal summer（May-September）. TRMM-ba...This study investigates the structure and propagation of intraseasonal sea surface temperature（SST） variability in the South China Sea（SCS） on the 30-60-day timescale during boreal summer（May-September）. TRMM-based SST, GODAS oceanic reanalysis and ERA-Interim atmospheric reanalysis datasets from 1998 to 2013 are used to examine quantitatively the atmospheric thermodynamic and oceanic dynamic mechanisms responsible for its formation. Power spectra show that the 30-60-day SST variability is predominant, accounting for 60% of the variance of the 10-90-day variability over most of the SCS. Composite analyses demonstrate that the 30-60-day SST variability is characterized by the alternate occurrence of basin-wide positive and negative SST anomalies in the SCS, with positive（negative） SST anomalies accompanied by anomalous northeasterlies（southwesterlies）. The transition and expansion of SST anomalies are driven by the monsoonal trough-ridge seesaw pattern that migrates northward from the equator to the northern SCS. Quantitative diagnosis of the composite mixed-layer heat budgets shows that, within a strong 30-60-day cycle, the atmospheric thermal forcing is indeed a dominant factor, with the mixed-layer net heat flux（MNHF） contributing around 60% of the total SST tendency, while vertical entrainment contributes more than 30%. However, the entrainment-induced SST tendency is sometimes as large as the MNHF-induced component, implying that ocean processes are sometimes as important as surface fluxes in generating the30-60-day SST variability in the SCS.展开更多
The project has as its aim the design and implementation of the control of temperature in the cockpit of the prototype of neonatal life support equipment ESVIN based on the international standard IEC 60601-2-19 concer...The project has as its aim the design and implementation of the control of temperature in the cockpit of the prototype of neonatal life support equipment ESVIN based on the international standard IEC 60601-2-19 concerning the basic security and operation of the neonatal incubators. The prototype has been developed and is important because the cockpit is a new concept of medical equipment of neonatal life support. There was a modeling of the system of heating of the incubator using the concepts of system identification with the purpose of finding a mathematical model that describes the dynamic behavior of the system. Then, design and implement the strategy of feedback control with digital PID （proportional-integral-derivative） algorithm. The model allowed the design and implementation of a digital PID controller that meets in a satisfactory manner with the requirements, in accordance with the international standard. The control system implemented in the neonatal incubator ESVIN improved the effectiveness of the neonatal life support equipment in regard to temperature controller of the cockpit.展开更多
Weak stratospheric polar vortex(WPV)events during winter months were investigated.WPV events were identified as being weakest in December,accompanied by the most dramatic increase in geopotential height over the polar...Weak stratospheric polar vortex(WPV)events during winter months were investigated.WPV events were identified as being weakest in December,accompanied by the most dramatic increase in geopotential height over the polar region.After the onset of a December WPV event,the dynamic processes influencing Eurasian temperature can be split into two separate periods.Period I(lag of 0-25 days)is referred to as the stratosphere-troposphere interactions period,as it is mainly characterized by stratospheric signals propagating downwards.In Period I,a stratospheric negative Northern Annular Mode(NAM)pattern associated with the WPV propagates downwards,inducing a negative NAM in the troposphere.The anomalous low centers over the Mediterranean and North Pacific bring cold advection to northern Eurasia,resulting in a north-cold-south-warm dipole pattern over Eurasia.The zero line between negative and positive temperature anomalies moves southwards during days 5-20.Stratospheric cold anomalies at midlatitudes propagate downwards to high latitudes in the troposphere and contribute to the dipole structure.During PeriodⅡ(lag of 25-40 days),as downward signals from the stratosphere have vanished,the dynamic processes mainly take place within the troposphere.Specifically,a wave train is initiated from the North Atlantic region to northern Europe.The propagation of wave activity flux intensifies a cyclonic anomaly over northern Europe,which brings cold advection to Scandinavia and warm advection to central Asia.Therefore,a northwest-cold-southeast-warm dipole structure occupies Eurasia and migrates southeastwards during this period.展开更多
The conditions of heating and cooling of piercing mandrels made of 4X5MFS steel of a three-roll screw mill 30-80 in the production of a closed cavity of steel vessels of small volume are determined.It is established t...The conditions of heating and cooling of piercing mandrels made of 4X5MFS steel of a three-roll screw mill 30-80 in the production of a closed cavity of steel vessels of small volume are determined.It is established that multiple cycles of heating up to 600℃ and cooling with water up to 80℃ for about 7 seconds/1 cycle lead to the formation of ridges,shells and cracks on the surface and in the volume of the tool.The loss of structural strength of the material leads to the breakdown of the mandrel during the stitching process.The technique and equipment of magnetic powder control have been developed to establish the dynamics of the growth of internal and external defects of mandrels.An equation is obtained that allows determining the increase in the number of defects in the sewing tool of a screw rolling mill.The technology of non-destructive testing made it possible to develop a rational plan for replacing the sewing mandrels,which allows for predicting the appearance of defects leading to a complex breakdown of the deforming tool at the NPO Pribor machine-building enterprise.展开更多
基金Supported by the National Natural Science Foundation of China (No.60574038) and the Open Project Program of the State KeyLaboratory of Bioreactor Engineering/ECUST.
文摘To track the rapidly changing temperature profiles of thermal cycling of polymerase chain reaction （PCR） accurately, an innovative feedforward variable structural proportional-integral-derivative （FVSPID） controller was developed. Based on the step response test data of the heat block, a reduced first order model was estabfished at different operating points. Based on the reduced model, the FVSPID controller combined a feedforward path with the variable structural proportional-integral-derivative （PID） control. The modified feedforward action provided directly the optimal predictive power for the desired setpoint to speed up the dynamic response. To cooperate with the feedforward action, a variable structural PID was applied, where the P mode was used in the case of the largest errors to speed up response, whereas the PD mode was used in the case of larger errors to suppress overshoot, and finally the PID mode was applied for small error conditions to eliminate the steady state offset. Experimental results illustrated that compared to the conventional PID controller, the FVSPID controller can not only reduce the time taken to complete a standard PCR protocol, but also improve the accuracy of gene amplification.
基金supported by the SOA Program on Global Change and Air–Sea Interactions (Grant No.GASI-IPOVAI03)the National Basic Research Program of China (Grant No.2014CB953902)+1 种基金the Natural Science Foundation of China (Grant Nos.91537103 and 41375087)the Priority Research Program of the Chinese Academy of Sciences (Grant Nos.QYZDY-SSWDQC018 and XDA11010402)
文摘This study investigates the structure and propagation of intraseasonal sea surface temperature（SST） variability in the South China Sea（SCS） on the 30-60-day timescale during boreal summer（May-September）. TRMM-based SST, GODAS oceanic reanalysis and ERA-Interim atmospheric reanalysis datasets from 1998 to 2013 are used to examine quantitatively the atmospheric thermodynamic and oceanic dynamic mechanisms responsible for its formation. Power spectra show that the 30-60-day SST variability is predominant, accounting for 60% of the variance of the 10-90-day variability over most of the SCS. Composite analyses demonstrate that the 30-60-day SST variability is characterized by the alternate occurrence of basin-wide positive and negative SST anomalies in the SCS, with positive（negative） SST anomalies accompanied by anomalous northeasterlies（southwesterlies）. The transition and expansion of SST anomalies are driven by the monsoonal trough-ridge seesaw pattern that migrates northward from the equator to the northern SCS. Quantitative diagnosis of the composite mixed-layer heat budgets shows that, within a strong 30-60-day cycle, the atmospheric thermal forcing is indeed a dominant factor, with the mixed-layer net heat flux（MNHF） contributing around 60% of the total SST tendency, while vertical entrainment contributes more than 30%. However, the entrainment-induced SST tendency is sometimes as large as the MNHF-induced component, implying that ocean processes are sometimes as important as surface fluxes in generating the30-60-day SST variability in the SCS.
文摘The project has as its aim the design and implementation of the control of temperature in the cockpit of the prototype of neonatal life support equipment ESVIN based on the international standard IEC 60601-2-19 concerning the basic security and operation of the neonatal incubators. The prototype has been developed and is important because the cockpit is a new concept of medical equipment of neonatal life support. There was a modeling of the system of heating of the incubator using the concepts of system identification with the purpose of finding a mathematical model that describes the dynamic behavior of the system. Then, design and implement the strategy of feedback control with digital PID （proportional-integral-derivative） algorithm. The model allowed the design and implementation of a digital PID controller that meets in a satisfactory manner with the requirements, in accordance with the international standard. The control system implemented in the neonatal incubator ESVIN improved the effectiveness of the neonatal life support equipment in regard to temperature controller of the cockpit.
基金supported by the National Natural Science Foundation of China [grant numbers 41730964,41575079,and 41421004]
文摘Weak stratospheric polar vortex(WPV)events during winter months were investigated.WPV events were identified as being weakest in December,accompanied by the most dramatic increase in geopotential height over the polar region.After the onset of a December WPV event,the dynamic processes influencing Eurasian temperature can be split into two separate periods.Period I(lag of 0-25 days)is referred to as the stratosphere-troposphere interactions period,as it is mainly characterized by stratospheric signals propagating downwards.In Period I,a stratospheric negative Northern Annular Mode(NAM)pattern associated with the WPV propagates downwards,inducing a negative NAM in the troposphere.The anomalous low centers over the Mediterranean and North Pacific bring cold advection to northern Eurasia,resulting in a north-cold-south-warm dipole pattern over Eurasia.The zero line between negative and positive temperature anomalies moves southwards during days 5-20.Stratospheric cold anomalies at midlatitudes propagate downwards to high latitudes in the troposphere and contribute to the dipole structure.During PeriodⅡ(lag of 25-40 days),as downward signals from the stratosphere have vanished,the dynamic processes mainly take place within the troposphere.Specifically,a wave train is initiated from the North Atlantic region to northern Europe.The propagation of wave activity flux intensifies a cyclonic anomaly over northern Europe,which brings cold advection to Scandinavia and warm advection to central Asia.Therefore,a northwest-cold-southeast-warm dipole structure occupies Eurasia and migrates southeastwards during this period.
文摘The conditions of heating and cooling of piercing mandrels made of 4X5MFS steel of a three-roll screw mill 30-80 in the production of a closed cavity of steel vessels of small volume are determined.It is established that multiple cycles of heating up to 600℃ and cooling with water up to 80℃ for about 7 seconds/1 cycle lead to the formation of ridges,shells and cracks on the surface and in the volume of the tool.The loss of structural strength of the material leads to the breakdown of the mandrel during the stitching process.The technique and equipment of magnetic powder control have been developed to establish the dynamics of the growth of internal and external defects of mandrels.An equation is obtained that allows determining the increase in the number of defects in the sewing tool of a screw rolling mill.The technology of non-destructive testing made it possible to develop a rational plan for replacing the sewing mandrels,which allows for predicting the appearance of defects leading to a complex breakdown of the deforming tool at the NPO Pribor machine-building enterprise.
