Polarization variable-angle synchronous fluorescence spectrometry was proposed to determine samples in turbid solution. A mixture of fluorescein, rhodamine 6G and rhodamine B was used to evaluate the technique. The ba...Polarization variable-angle synchronous fluorescence spectrometry was proposed to determine samples in turbid solution. A mixture of fluorescein, rhodamine 6G and rhodamine B was used to evaluate the technique. The background caused by scattering light was decreased remarkably. The limits of detection were 0.6 ng/ml for fluorescein, 2.3 ng/ml for rhodamine 6G and 4.1 ng/ml for rhodamine B, respectively.展开更多
Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate （SGR） and chlorophyll fluores...Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate （SGR） and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50, and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence （F_v/F_m） were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO_4-P. The photosynthetic capacity of photosystem Ⅱ （PSⅡ） was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO_3-N concentrations were greater than 1.0 mmol/L and when PO4-P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera, these data contribute important information to ensure optimal results.展开更多
文摘Polarization variable-angle synchronous fluorescence spectrometry was proposed to determine samples in turbid solution. A mixture of fluorescein, rhodamine 6G and rhodamine B was used to evaluate the technique. The background caused by scattering light was decreased remarkably. The limits of detection were 0.6 ng/ml for fluorescein, 2.3 ng/ml for rhodamine 6G and 4.1 ng/ml for rhodamine B, respectively.
基金Supported by the Technology Program of Basic Research of Qingdao(No.12-1-4-8-(2)-jch)
文摘Caulerpa lentillifera is a green algae that distributes worldwide and is cultivated for food. We assessed vegetative propagation of C. lentillifera by measuring the specific growth rate （SGR） and chlorophyll fluorescence of the green algae cultured at different salinities and nutrient levels. The results indicated that C. lentillifera can survive in salinities ranging from 20 to 50, and can develop at salinities of 30 to 40. The maximum SGR for C. lentillifera occurred at a salinity of 35. Both chlorophyll content and the ratio of variable to maximum fluorescence （F_v/F_m） were also at a maximum at a salinity of 35. Photosynthesis was inhibited in salinities greater than 45 and less than 25. Both the maximum SGR and maximum chlorophyll content were found in algae treated with a concentration of 0.5 mmol/L of NO3-N and 0.1 mmol/L of PO_4-P. The photosynthetic capacity of photosystem Ⅱ （PSⅡ） was inhibited in cultures of C. lentillifera at high nutrient levels. This occurred when NO_3-N concentrations were greater than 1.0 mmol/L and when PO4-P concentrations were at 0.4 mmol/L. As there is strong need for large-scale cultivation of C. lentillifera, these data contribute important information to ensure optimal results.
