Associations of genome-wide structural variations with phenotypic differences in cross-bred Eurasian pigs

Background During approximately 10,000 years of domestication and selection,a large number of structural variations(SVs)have emerged in the genome of pig breeds,profoundly influencing their phenotypes and the ability to adapt to the local environment.SVs(≥50 bp)are widely distributed in the genome,mainly in the form of insertion(INS),mobile element insertion(MEI),deletion(DEL),duplication(DUP),inversion(INV),and translocation(TRA).While studies have investigated the SVs in pig genomes,genome-wide association studies(GWAS)-based on SVs have been rarely conducted.Results Here,we obtained a high-quality SV map containing 123,151 SVs from 15 Large White and 15 Min pigs through integrating the power of several SV tools,with 53.95%of the SVs being reported for the first time.These high-quality SVs were used to recover the population genetic structure,confirming the accuracy of genotyping.Potential functional SV loci were then identified based on positional effects and breed stratification.Finally,GWAS were performed for 36 traits by genotyping the screened potential causal loci in the F2 population according to their corresponding genomic positions.We identified a large number of loci involved in 8 carcass traits and 6 skeletal traits on chromosome 7,with FKBP5 containing the most significant SV locus for almost all traits.In addition,we found several significant loci in intramuscular fat,abdominal circumference,heart weight,and liver weight,etc.Conclusions We constructed a high-quality SV map using high-coverage sequencing data and then analyzed them by performing GWAS for 25 carcass traits,7 skeletal traits,and 4 meat quality traits to determine that SVs may affect body size between European and Chinese pig breeds.

Inter-annual variations of dissolved oxygen and hypoxia off the northern Changjiang River(Yangtze River) Estuary in summer from 1997 to 2014

Hypoxia off the Changjiang River Estuary has been the subject of much attention,yet systematic observations have been lacking,resulting in a lack of knowledge regarding its long-term change and drivers.By revisiting the repeated surveys of dissolved oxygen(DO) and other relevant hydrographic parameters along the section from the Changjiang River Estuary to the Jeju-do in the summer from 1997 to 2014,rather different trends were revealed for the dual low-DO cores.The nearshore low-DO core,located close to the river mouth and relatively stable,shows that hypoxia has become more severe with the lowest DO descen ding at a rate of -0.07 mg/(L·a) and the thickness of low-DO zone rising at a rate of 0.43 m/a.The offshore core,centered around 40-m isobath but moving back and forth between 123.5°-125°E,shows large fluctuations in the minimum DO concentration,with the thickness of low-DO zone falling at a rate of -1.55 m/a.The probable factors affecting the minimum DO concentration in the two regions also vary.In the nearshore region,the decreasing minimum DO is driven by the increase in both stratification and primary productivity,with the enhanced extension of the Changjiang River Diluted Water(CDW) strengthening stratification.In the offshore region,the fluctuating trend of the minimum DO concentration indicates that both DO loss and DO supplement are distinct.The DO loss is primarily attributed to bottom apparent oxygen utilization caused by the organic matter decay and is also relevant to the advection of low-DO water from the nearshore region.The DO supplement is primarily due to weakened stratification.Our analysis also shows that the minimum DO concentration in the nearshore region was extremely low in 1998,2003,2007 and 2010,related to El Ni?o signal in these summers.

Spatiotemporal variations,influencing factors,and configurational promotion paths of energy efficiency in China’s cities

The promotion of energy efficiency(EE)helps address energy constraints and promote environmental sustainability.This study comprehensively explores the spatiotemporal variations,influencing factors,and configuration promotion paths of EE in 284 Chinese cities during 2003‒2019 using the global super-efficiency minimum distance to strong efficient frontier(G-S-MinDS),exploratory spatial data analysis(ESDA),multiscale geographically weighted regression(MGWR),and fuzzy set qualitative comparative analysis(fsQCA)methods.The findings are:①China’s cities have an annual average EE of 0.658 with a growth rate of 0.53%,showing considerable promotion potential.②Industrial structure optimization,population agglomeration,economic development,and increased green coverage contribute positively,while government intervention and openness hinder China’s urban EE.③Four configurational promotion paths for enhancing China’s urban EE are identified,where among those paths population density is a core condition,while government intervention is not.This study provides valuable insights into substantially improving urban EE,emphasizing the need for targeted policies to address energy and environmental crises in China.

Insights into genetic diversity and phenotypic variations in domestic geese through comprehensive population and pan-genome analysis

Background Domestic goose breeds are descended from either the Swan goose(Anser cygnoides)or the Greylag goose(Anser anser),exhibiting variations in body size,reproductive performance,egg production,feather color,and other phenotypic traits.Constructing a pan-genome facilitates a thorough identification of genetic variations,thereby deepening our comprehension of the molecular mechanisms underlying genetic diversity and phenotypic variability.Results To comprehensively facilitate population genomic and pan-genomic analyses in geese,we embarked on the task of 659 geese whole genome resequencing data and compiling a database of 155 RNA-seq samples.By constructing the pan-genome for geese,we generated non-reference contigs totaling 612 Mb,unveiling a collection of 2,813 novel genes and pinpointing 15,567 core genes,1,324 softcore genes,2,734 shell genes,and 878 cloud genes in goose genomes.Furthermore,we detected an 81.97 Mb genomic region showing signs of genome selection,encompassing the TGFBR2 gene correlated with variations in body weight among geese.Genome-wide association studies utilizing single nucleotide polymorphisms(SNPs)and presence-absence variation revealed significant genomic associations with various goose meat quality,reproductive,and body composition traits.For instance,a gene encoding the SVEP1 protein was linked to carcass oblique length,and a distinct gene-CDS haplotype of the SVEP1 gene exhibited an association with carcass oblique length.Notably,the pan-genome analysis revealed enrichment of variable genes in the“hair follicle maturation”Gene Ontology term,potentially linked to the selection of feather-related traits in geese.A gene presence-absence variation analysis suggested a reduced frequency of genes associated with“regulation of heart contraction”in domesticated geese compared to their wild counterparts.Our study provided novel insights into gene expression features and functions by integrating gene expression patterns across multiple organs and tissues in geese and analyzing population variation.Conclusion This accomplishment originates from the discernment of a multitude of selection signals and candidate genes associated with a wide array of traits,thereby markedly enhancing our understanding of the processes underlying domestication and breeding in geese.Moreover,assembling the pan-genome for geese has yielded a comprehensive apprehension of the goose genome,establishing it as an indispensable asset poised to offer innovative viewpoints and make substantial contributions to future geese breeding initiatives.

Spatiotemporal variations of parameters of internal solitary waves in the northern South China Sea

The dynamic parameters for internal solitary waves(ISWs)derived from the extended Korteweg-de Vries(eKdV)equation play an important role in the understanding and prediction of ISWs.The spatiotemporal variations of the dynamic parameters of the ISWs in the northern South China Sea(SCS)were studied based on the reanalysis of long-term temperature and salinity datasets.The results for spectrum analysis show that there are definite geographical differences for the periodic variation of the parameters:in shallow water,all parameters vary with a wave period of one year,while in deep water wave components of the parameters at other frequencies exist.Using wavelet analysis,the wavelet power spectral densities in deep water exhibited an inter-annual variation pattern.For example,the wave component of the dispersion coefficient with a wave period of about half a year reached its power peak once every two years.Based on previous work,this inter-annual variation pattern was deduced to be caused by dynamic processes.In further work on the regulatory mechanisms,empirical orthogonal function(EOF)decomposition was performed.It was found that the modes of the dispersion coefficient have different geographical distributions,explaining the reason why the wave components in different frequencies appeared in different locations.The numerical simulation results confirm that the variations in the parameters of the ISWs derived from the eKdV equation could affect the waveforms significantly because of changes in the polarity of the ISWs.Therefore,the periodic variations of the dynamic parameters are related to the geographical location because of dynamic processes operating.

High Prevalence of Anatomical Variations and Anomalies of the Coronary Arteries Detected by CT Angiography in Symptomatic Patients

Objective:Coronary artery anatomical variations and anomalies are an important topic due to their potential clinical manifestations.This study aims to investigate the prevalence of coronary artery anatomical variations and anomalies in symptomatic patients with coronary computed tomography angiography(CCTA).Methods:This is a retrospective study that included all symptomatic patients who had CCTA in a tertiary care hospital in Saudi Arabia during a period of seven years.Results:The total number of included patients was 507(60%males)with a mean age of 57.4 years.Approximately 41%had luminal stenoses,averaging 49.7%.The total num-ber of patients with coronary anatomical variations(CAV)and coronary artery anomalies(CAA)was 217(43%).CAV prevalence was 26%,which included 14%non-right coronary dominance,5%short left main coronary artery(LMCA),and 7%division variations(trifurcation and quadrifurcarion)of the LMCA.The prevalence of CAA was 29%,which included 5%origin anomalies,22%myocardial bridge,and 2%course anomalies.Conclusions:A high prevalence of coronary artery anatomic variations and anomalies in symptomatic patients is reported in this study.Systematic reviews,meta-analyses,reporting guidelines,and unified definitions and classifications of cor-onary variations and anomalies are lacking in the literature,presenting potential opportunities for future research and publications.

Temporal and spatial variations hydrochemical components and driving factors in Baiyangdian Lake in the Northern Plain of China

Understanding the temporal and spatial variation of hydrochemical components in large freshwater lakes is crucial for effective management and conversation.In this study,we identify the temporalspatial characteristics and driving factors of the hydrochemical components in Baiyangdian Lake using geochemical methods(Gibbs diagram,Piper diagram and End-element diagram of ion ratio)and multivariate statistical techniques(Principal component analysis and Correlation analysis).16 sets of samples were collected from Baiyangdian Lake in May(normal season),July(flood season),and December(dry season)of 2022.Results indicate significant spatial variation in Nat,ci,SO and NO,,suggesting a strong influence of human activities.Cation concentrations exhibit greater seasonal variation in the dry season compared to the flood season,while the concentrations of the four anions show inconsistent seasonal changes due to the combined effects of river water chemical composition and human activities.The hydrochemical type of Baiyangdian Lake is primarily HCO,Cl-Na.Ca,Mg*and HCO,originate mainly from silicate and carbonate rock dissolution,while Kt,Nat and CI originate mainly from sewage and salt dissolution in sediments.SO42 may mainly stem from industrial wastewater,while NO,primarily originates from animal feces and domestic sewage.Through the use of Principal Component Analysis,it is identified that water-rock interaction(silicate and carbonate rocks dissolution,and dissolution of salt in sediments),carbonate sedimentation,sewage,agricultural fertilizer and manure,and nitrification are the main driving factors of the variation of hydrochemical components of Baiyangdian Lake across three hydrological seasons.These findings suggest the need for effective control of substandard domestic sewage discharge,optimization of agricultural fertilization strategies,and proper management of animal manure to comprehensively improve the water environment in Baiyangdian Lake.

Variations in evaporation from water surfaces along the margins of the Badain Jaran Desert over nearly 60 years and influencing factors

Based on meteorological data collected over nearly 60 years(1960-2017)from four national meteorological stations along the margins of the Badain Jaran Desert,this study analyzed the spatiotemporal variations in evaporation from water surfaces and identified the dominant controlling factors.Methods used included linear trend analysis,linear tendency estimation,the departure method,the rank correlation coefficient-based method,and Multiple Linear Regression(MLR).Results indicate notable spatiotemporal differences in evaporation distribution and evolution.Spatially,average annual evaporation exhibited a pronounced altitude effect,decreasing at a rate of about 8.23 mm/m from east to west with increasing altitude.Temporally,annual evaporation showed significant upward trends after 1996 at the northeastern(Guaizi Lake)and western(Dingxin)margins,with rates of 132 mm/10a and 105 mm/10a,respectively.Conversely,along the northwestern(Ejina Banner)and southern(Alxa Right Banner)margins of the desert,an evaporation paradox was observed,with annual evaporation trending downward at rates of 162 mm/10a and 187 mm/10a,respectively,especially after 1987.The dominant factors controlling evaporation varied spatially:Average annual temperature and relative humidity influended the western margin(Dingxin),average annual temperature was the key factor for the northeastern margin(Guaizi Lake),and average wind speed was crucial for the northern(Ejina Banner)and southern(Alxa Right Banner)margins.

Relationship Between Gene-Phenotype and Clinical Manifestations of Chromosomal Copy Number Variations Indicated by Non-Invasive Prenatal Testing

Objective:To analyze the clinical value of non-invasive prenatal testing(NIPT)in detecting chromosomal copy number variations(CNVs)and to explore the relationship between gene expression and clinical manifestations of chromosomal copy number variations.Methods:3551 naturally conceived singleton pregnant women who underwent NIPT were included in this study.The NIPT revealed abnormalities other than sex chromosome abnormalities and trisomy 13,18,and 21.Pregnant women with chromosome copy number variations underwent genetic counseling and prenatal ultrasound examination.Interventional prenatal diagnosis and chromosome microarray analysis(CMA)were performed.The clinical phenotypes and pregnancy outcomes of different prenatal diagnoses were analyzed.Additionally,a follow-up was conducted by telephone to track fetal development after birth,at six months,and one year post-birth.Results:A total of 53 cases among 3551 cases showed chromosomal copy number variation.Interventional prenatal diagnosis was performed in 36 cases:27 cases were negative and 8 were consistent with the NIPT test results.This indicates that NIPT’s positive predictive value(PPV)in CNVs is 22.22%.Conclusion:NIPT has certain clinical significance in screening chromosome copy number variations and is expected to become a routine screening for chromosomal microdeletions and microduplications.However,further interventional prenatal diagnosis is still needed to identify fetal CNVs.

Response of the North Pacific Storm Track Activity in the Cold Season to Multi-scale Oceanic Variations of Kuroshio Extension System: A Statistical Assessment

In this paper,a statistical method called Generalized Equilibrium Feedback Analysis(GEFA)is used to investigate the responses of the North Pacific Storm Track(NPST)in the cold season to the multi-scale oceanic variations of the Kuroshio Extension(KE)system,including its large-scale variation,oceanic front meridional shift,and mesoscale eddy activity.Results show that in the cold season from the lower to the upper troposphere,the KE large-scale variation significantly weakens the storm track activity over the central North Pacific south of 30°N.The northward shift of the KE front significantly strengthens the storm track activity over the western and central North Pacific south of 40°N,resulting in a southward shift of the NPST.In contrast,the NPST response to KE mesoscale eddy activity is not so significant and relatively shallow,which only shows some significant positive signals near the dateline in the lower and middle troposphere.Furthermore,it is found that baroclinicity and baroclinic energy conversion play an important role in the formation of the NPST response to the KE multi-scale oceanic variations.

Seasonal variations in glacier velocity in the High Mountain Asia region during 2015–2020

Velocity is an important component of glacier dynamics and directly reflects the response of glaciers to climate change.As a result,an accurate determination of seasonal variation in glacier velocity is very important in understanding the annual variation in glacier dynamics.However,few studies of glacier velocity in the High Mountain Asia(HMA)region were done.Along these lines,in this work,based on Sentinel-1 glacier velocity data,the distribution of glacier velocity in the HMA region was plotted and their seasonal variations during 2015-2020 were systematically analysed.The average glacier velocity in the HMA region was 0.053 m/d,and was positively correlated with the glacier area and slope.Glaciers in the Karakoram Mountains had the fastest average flow velocity(0.060 m/d),where the glaciers exhibited the largest average area and average slope.Moreover,glaciers in the GangdisêMountains had the slowest velocity(0.022 m/d)and the smallest average glacier area.The glacier flows were the fastest in spring(0.058 m/d),followed by summer(0.050 m/d),autumn(0.041 m/d),and winter(0.040 m/d).In addition,the glacier flows were the maximum in May,being 1.4 times of the annual average velocity.In some areas,such as the Qilian,Altun,Tibetan Interior,Eastern Kunlun,and Western Kunlun mountains,the peak glacier velocities appeared in June and July.The glacier velocity in the HMA region decreased in midsummer and reached the minimum in December when it was 75%of the annual average.These results highlight the role of meltwater in the seasonal variation in glacier flows in late spring and early summer.The seasonal velocity variation of lake-terminating glaciers was similar to that of land-terminating ones,but the former flowed faster.The velocity difference close to the mass balance line between the lake-and land-terminating glaciers was obviously greater in spring than in other seasons.In summer,the difference between the lake-and land-terminating glaciers at a normalized distance of 0.05-0.40 from the terminus was significantly greater than those of other seasons.The velocity difference between the lake-and land-terminating glaciers is closely related to the variable of ice thickness,and also to the frictional force of the terminal base reduced by proglacial lakes.Thus,it can be concluded that in addition to the variation of the glacier thickness and viscosity,the variation of glacier water input also plays a key role in the seasonal variation of glacier velocity.

Eff ects of environmental factors on the temporal and spatial variations in branch and leaf CO_(2)effl ux of Larix gmelinii var.principis-rupprechtii Mayr

The CO_(2)effl ux of branches and leaves plays an important role in ecosystem carbon balance.Using a carbon fl ux system,the effl ux of Larix gmelinii var.principisrupprechtii(Dahurian larch)was investigated in 27 years(immature),31 years(near-mature),and 47 years(mature)stands at diurnal,seasonal,and spatial scales(direction and height)as well as its connection with environmental factors from May to October 2020.Diurnal variation in effl ux was a single peak,and the maximum occurring between 14:00 and 16:00.Seasonal variation also exhibited a single peak,with the maximum in late July and the minimum in early October.From May to September,effl ux on the south side was the largest among the three stands,and mean values on the south side of 27 year-old,31 year-old,and 47 year-old trees were 0.50,0.97 and 1.05μmol·m^(–2)·s^(–1),respectively.The minimum occurred on the north side.Except for the maximum in July and September in the 27 year-old stand in the middle of the canopy,the maximum effl ux in the upper canopy,and the means in the 27 year-old,31 year-old,and 47 year-old stands were 0.49,0.96 and 1.04μmol·m^(-2)·s^(-1),respectively;the minimum occurred in the lower canopy.Temperatures and relative humidity infl uenced seasonal variations in effl ux.Seasonal variation in temperature sensitivity coeffi cient(Q 10)was opposite that of temperature,increasing with decreasing temperature.At the spatial scale,maximum Q 10 occurred in the mid canopy.With the effl ux and temperature data in diff erent locations,it is possible to better estimate effl ux variations in each stand.

Variations and relations between chlorophyll concentrations and physical-ecological processes near the West Antarctic Peninsula

The West Antarctic Peninsula(WAP)region is one of the most productive marine ecosystems in the Southern Ocean that support the food web for phytoplankton,krill spawning or recruitment and several krill consumers at higher-trophic level like penguins and Antarctic fur seals.Characterized by channels and islands,the complex topography of the WAP generates interconnected circulation patterns,strongly influencing vertical stratification,nutrient availability and distribution of marine organisms.Additionally,rapid climate change associated with major climate modes like the Southern Annular Mode(SAM)and El Niño-Southern Oscillation(ENSO)has significant effects on long-term variations of physical environments and biological production.The objective of this study is to reveal the spatial-temporal variations of phytoplankton biomass in the WAP region and the modulating physical-ecological processes.By using 9-year hydrographic and ecological data of five transects collected by the Palmer Long-Term Ecosystem Research,the horizontal and vertical distributions of several physical and ecological properties,with a particular focus on chlorophyll(Chl)concentration were explored.Regression analysis among area-averaged properties and properties at single stations was performed to reveal the relationship between the interannual variations of physical and ecological processes.The correlation results showed that Chl concentration exhibited a positive relationship with both the circumpolar deep water(CDW)intrusion and vertical stratification,but showed a negative correlation with SAM at some specific stations.However,certain processes or mechanisms may only be dominant for specific stations and not applicable to the entire region.No single physical or ecological factors have been found to significantly influence the Chl distribution throughout the WAP region,which may be attributed to the heterogeneity of sea ice conditions,geometry and hydrodynamic features as well as variations in nutrient sources.

Spatial and Temporal Variations of Dissolved Inorganic Nutrients and Relationship with Phytoplankton Density in Coastal Water of Kudat, Sabah, Malaysia

Coastal areas of Kudat are dominated by fisheries activities. Development of infrastructure for fishing facilities like jetty, landing centre and other human activities can increase the loading of nutrients in coastal area. High load of nutrients accelerates the blooming of phytoplankton and in long run creates eutrophication. This study was conducted to determine the temporal and spatial variations of nutrients and relationship of phytoplankton density in coastal water of Kudat. Five stations were selected with three replicated in the study area. Phytoplankton samples, water samples and in situ environmental parameters were collected from May 2019 to February 2020 (10 months). The highest concentration of nitrate (NO3) was observed in May 2019, but the highest concentration of phosphate (PO4) observed in July 2019. On the other hand, higher concentrations of phosphate (PO4) were determined than the concentration of nitrate (NO3) in all stations during study period. The distribution of nutrients is due to monsoonal runoff from surrounding areas as well due to anthropogenic activities. Among the 21 species three of them are from harmful algal species and but dominated by diatoms. Poor relationship observed among the dissolved inorganic nutrients and phytoplankton density, indicates that the relationship does not depend on only nutrients but with favourable environmental parameters. Anthropogenic activity can lead to excessive load of nutrients in Kudat coastal water and in long run cause eutrophication problem in ecosystem with potentially larger economic impacts in a long run period.

Variations in chlorophyll content, stomatal conductance, and photosynthesis in Setaria EMS mutants

Chlorophyll (Chl) content,especially Chl b content,and stomatal conductance (G_s) are the key factors affecting the net photosynthetic rate (P_n).Setaria italica,a diploid C_4 panicoid species with a simple genome and high transformation efficiency,has been widely accepted as a model in photosynthesis and drought-tolerance research.The current study characterized Chl content,G_s,and P_n of 48 Setaria mutants induced by ethyl methanesulfonate.A total of 24,34,and 35 mutants had significant variations in Chl content,G_s,and P_n,respectively.Correlation analysis showed a positive correlation between increased G_s and increased P_n,and a weak correlation between decreased Chl b content and decreased P_n was also found.Remarkably,two mutants behaved with significantly decreased Chl b content but increased P_n compared to Yugu 1.Seven mutants behaved with significantly decreased G_s but did not decrease P_(n )compared to Yugu 1.The current study thus identified various genetic lines,further exploration of which would be beneficial to elucidate the relationship between Chl content,G_s,and P_n and the mechanism underlying why C_4 species are efficient at photosynthesis and water saving.

Diversity on the Interannual Variations of Spring Monthly Precipitation in Southern China and the Associated Tropical Sea Surface Temperature Anomalies

There is a continuous and relatively stable rainy period every spring in southern China(SC).This spring precipitation process is a unique weather and climate phenomenon in East Asia.Previously,the variation characteristics and associated mechanisms of this precipitation process have been mostly discussed from the perspective of seasonal mean.Based on the observed and reanalysis datasets from 1982 to 2021,this study investigates the diversity of the interannual variations of monthly precipitation in spring in SC,and focuses on the potential influence of the tropical sea surface temperature(SST)anomalies.The results show that the interannual variations of monthly precipitation in spring in SC have significant differences,and the correlations between each two months are very weak.All the interannual variations of precipitation in three months are related to a similar western North Pacific anomalous anticyclone(WNPAC),and the southwesterlies at the western flank of WNPAC bring abundant water vapor for the precipitation in SC.However,the WNPAC is influenced by tropical SST anomalies in different regions each month.The interannual variation of precipitation in March in SC is mainly influenced by the signal of El Nino-Southern Oscillation,and the associated SST anomalies in the equatorial central-eastern Pacific regulate the WNPAC through the Pacific-East Asia(PEA)teleconnection.In contrast,the WNPAC associated with the interannual variation of precipitation in April can be affected by the SST anomalies in the northwestern equatorial Pacific through a thermally induced Rossby wave response.The interannual variation of precipitation in May is regulated by the SST anomalies around the western Maritime Continent,which stimulates the development of low-level anomalous anticyclones over the South China Sea and east of the Philippine Sea by driving anomalous meridional vertical circulation.

Quantitative Study of the Morphological Variations among Dzieduszyckia Populations from the Devonian Rongxian Formation,South China and their Influencing Factors

The rhynchonellide brachiopod Dzieduszyckia Siemiradzki is widely distributed in the Lower Famennian strata,and exhibits a wide range of morphological variations among populations.1272 autochthonous Devonian Rongxian Dzieduszyckia fossils were collected from three populations in South China,namely the Dazhai,Dalong,and Longmi sections.A series of quantitative morphological analyses were conducted to understand the morphological variation among populations and identify the triggers responsible for such variations.The results show that the Dazhai population differs significantly in morphospace from the Dalong and Longmi populations,while the latter two exhibit more similar morphological features,suggesting that geographic distribution may play a key role in affecting Dzieduszyckia morphology.The Longmi population shows a distinct morphospace from the Dazhai population and an overlapped morphospace with the Dalong population,indicating that habitat may not be the controlling factor.Lastly,the Dazhai and Dalong populations share some morphological similarities,but the Dazhai and Longmi populations occupy completely different morphospaces,indicating that evolution had a greater impact on phenotypic divergence than habitat.Our findings suggest that geographic distribution is the predominant factor influencing the morphological variation of Dzieduszyckia,while evolution and living habitat are less significant,the former of which might play a more important role.

Variations of extreme temperature in the Mount Qomolangma region in China during 1971-2020

According to observational daily temperature data from the meteorological stations during 1971-2020,the variations of the extreme temperature event in the Mount Qomolangma(also known as Mount Everest) region in China have been analyzed using statistical methods.The extreme temperature indices recommended by the World Meteorological Organization are selected to describe the extreme temperature event.The RClimDEX 1.0 software is used to calculate the extreme temperature indices.There are no tropical nights,and just three summer days at the last 50 years.The frost days are the main extreme temperature events all year round.The temperature in the north slope is more extreme than that in the south slope of the Mount Qomolangma.There is remarkable decadal variation for the extreme temperature indices except diurnal temperature range.There are the warm extremes increasing,however,the cold extremes decreasing with the decadal lapse,which is more remarkable into the 21^(th) century.The tendencies for the extreme temperature indices in the north slope are consistent with those in the south slope.There are statistically significant trends for most extreme temperature indices during the study period.It shows that the warm extremes would be more prominent in the future with the global continued warming.The abrupt changes of the extreme temperature index have occurred mainly in the 20^(th) century especially from the mid to late 1980s and 1990s.The periodic changes in the south slope do not synchronize those in the north slope for the most extreme temperature indices.It is different for most extreme temperature indices between the south and north slope,which has demonstrated that the regional or local changes are not neglectable for extreme temperature research.The results of this study are also the consistent response of extreme temperature event to the global warming.

Diel,seasonal,and annual variations of fish assemblages in intertidal creeks of the Changjiang River estuary

The use patterns of salt marsh habitats by fish assemblages were investigated in two welldeveloped intertidal creeks in the Changjiang(Yangtze)River estuary on spatial and temporal scales.Samples were collected using fyke nets at two sites during day and night in each season throughout four years.Notable changes in fish assemblages were detected over seasonal and interannual cycles,with many more marine species present in winter assemblages,whereas freshwater and estuarine species characterized the other seasonal samplings.The appearance of catadromous species in intertidal creeks mainly depended upon their specific physiological requirements,such as spawning migration.No significant diel shifts were observed in fish assemblages.This diel pattern may be a combined consequence of avian predation pressure and other drivers(i.e.,inherent living behaviors,food availability,and predation by piscivorous fishes).The spatial difference in fish community was insignificant even though the two intertidal creeks were different in size.Intertidal creeks with different size could possess similar ecological value for fish fauna.Redundancy analysis(RDA)showed that biological factors exerted greater effects on fish assemblages than physical variables.Temporal variations were strongly facilitated by food availability,but negatively impacted by avian predation.Salinity,water temperature,and river runoff had weak influence on temporal variations of fish communities.The lengthfrequency distribution showed that the fish collected in these two sites mainly consisted of young-of-year and juvenile fish,which confirmed that the creeks provided important nursery and forage roles in the process of fish ontogenetic development.Further,we proposed that salt marsh conservation management should be equally applied to all well-developed intertidal creeks regardless of creek size.

Interdecadal Variations of the March Atmospheric Heat Source over the Southeast Asian Low-Latitude Highlands

Based on the fifth-generation reanalysis dataset from the European Centre for Medium-Range Weather Forecasts for 1979–2019,we investigated the effects of the circumglobal teleconnection(CGT)on the interdecadal variation of the March atmospheric heat source(AHS)over the Southeast Asian low-latitude highlands(SEALLH).The dominant mode of the March AHS over the SEALLH features a monopole structure with an 8–11-year period.Decadal variations in the AHS make an important contribution to the 11-year low-pass filtered component of the AHS index,whichexplains 54.3%of the total variance.The CGT shows a clear interdecadal variation,which explains 59.3%of the total variance.The March AHS over the SEALLH is significantly related to the CGT on interdecadal timescales.When the CGT is optimally excited by a significant cyclonic vorticity source near northern Africa(i.e.,in its positive phase),the SEALLH is dominated by anomalous southerly winds and ascending motions on the east of the anomalous cyclone.The enhanced advection and upward transfer result in a high-enthalpy air mass that converges into and condenses over the SEALLH,leading to a largerthan-average March AHS over this region.The key physical processes revealed by this diagnostic analysis are supported by numerical experiments.