Single-cell profiling of the pig cecum at various developmental stages

The gastrointestinal tract is essential for food digestion,nutrient absorption,waste elimination,and microbial defense.Single-cell transcriptome profiling of the intestinal tract has greatly enriched our understanding of cellular diversity,functional heterogeneity,and their importance in intestinal tract development and disease.Although such profiling has been extensively conducted in humans and mice,the single-cell gene expression landscape of the pig cecum remains unexplored.Here,single-cell RNA sequencing was performed on 45572 cells obtained from seven cecal samples in pigs at four different developmental stages(days(D)30,42,150,and 730).Analysis revealed 12 major cell types and 38 subtypes,as well as their distinctive genes,transcription factors,and regulons,many of which were conserved in humans.An increase in the relative proportions of CD8^(+)T and Granzyme A(low expression)natural killer T cells(GZMA^(low)NKT)cells and a decrease in the relative proportions of epithelial stem cells,Tregs,RHEX^(+)T cells,and plasmacytoid dendritic cells(pDCs)were noted across the developmental stages.Moreover,the post-weaning period exhibited an up-regulation in mitochondrial genes,COX2 and ND2,as well as genes involved in immune activation in multiple cell types.Cell-cell crosstalk analysis indicated that IBP6^(+)fibroblasts were the main signal senders at D30,whereas IBP6^(−)fibroblasts assumed this role at the other stages.NKT cells established interactions with epithelial cells and IBP6^(+)fibroblasts in the D730 cecum through mediation of GZMA-F2RL1/F2RL2 pairs.This study provides valuable insights into cellular heterogeneity and function in the pig cecum at different development stages.

Multifunctional AlPO_(4)reconstructed LiMn_(2)O_(4)surface for electrochemical lithium extraction from brine

LiMn_(2)O_(4)(LMO)electrochemical lithium-ion pump has gained widespread attention due to its green,high efficiency,and low energy consumption in selectively extracting lithium from brine.However,collapse of crystal structure and loss of lithium extraction capacity caused by Mn dissolution loss limits its industrialized application.Hence,a multifunctional coating was developed by depositing amorphous AlPO_(4)on the surface of LMO using sol-gel method.The characterization and electrochemical performance test provided insights into the mechanism of Li^(+)embedment and de-embedment and revealed that multifunctional AlPO_(4)can reconstruct the physical and chemical state of LMO surface to improve the interface hydrophilicity,promote the transport of Li^(+),strengthen cycle stability.Remarkably,after 20 cycles,the capacity retention rate of 0.5AP-LMO reached 93.6%with only 0.147%Mn dissolution loss.The average Li^(+)release capacity of 0.5AP-LMO//Ag system in simulated brine is 28.77 mg/(g h),which is 90.4%higher than LMO.Encouragingly,even in the more complex Zabuye real brine,0.5AP-LMO//Ag can still maintain excellent lithium extraction performance.These results indicate that the 0.5AP-LMO//Ag lithium-ion pump shows promising potential as a Li^(+)selective extraction system.

Ru(Ⅲ)-catalyzed construction of variously substituted quinolines from 2-aminoaromatic aldehydes(ketones)and isoxazoles:Isoxazoles as cyclization reagent and cyano sources

A Ru(Ⅲ)-catalyzed annulation reaction of 2-aminoaromatic aldehydes(ketones)and isoxazoles to afford diverse 3-cyanoquinolines has been developed.Notably,isoxazole acted as a cyclization reagent and nontoxic cyano source via N-O bond cleavage and fragmentation.Variously substituted(especially 6-or 7-substituted)quinolines could be easily afforded.This procedure features wide functional group compatibility,efficiency and avoiding toxic cyano source.Meanwhile,this protocol could be successfully applied to scale-up synthesis.Further chemical transformations of 3-cyanoquinoline could give some valuable skeletons,demonstrating its potential in synthetic application.

Measurement Uncertainty Analysis of the Rotary-scan Method for the Measurable Dimension of Cylindrical Workpieces

The measurement uncertainty analysis is carried out to investigate the measurable dimensions of cylindrical workpieces by the rotary-scan method in this paper.Due to the difficult alignment of the workpiece with a diameter of less than 3 mm by the rotary scan method,the measurement uncertainty of the cylindrical workpiece with a diameter of 3 mm and length of 50 mm which is measured by a roundness measuring machine,is evaluated according to GUM(Guide to the Expression of Uncertainty in Measurement)as an example.Since the uncertainty caused by the eccentricity of the measured workpiece is different with the dimension changing,the measurement uncertainty of cylindrical workpieces with other dimensions can be evaluated the same as the diameter of 3 mm but with different eccentricity.Measurement uncertainty caused by different eccentricities concerning the dimension of the measured cylindrical workpiece is set to simulate the evaluations.Compared to the target value of the measurement uncertainty of 0.1μm,the measurable dimensions of the cylindrical workpiece can be obtained.Experiments and analysis are presented to quantitatively evaluate the reliability of the rotary-scan method for the roundness measurement of cylindrical workpieces.

Fluoridation routes,function mechanism and application of fluorinated/fluorine-doped nanocarbon-based materials for various batteries:A review

With the popularity and widespread applications of electronics,higher demands are being placed on the performance of battery materials.Due to the large difference in electronegativity between fluorine and carbon atoms,doping fluorine atoms in nanocarbon-based materials is considered an effective way to improve the performance of used battery.However,there is still a blank in the systematic review of the mechanism and research progress of fluorine-doped nanostructured carbon materials in various batteries.In this review,the synthetic routes of fluorinated/fluorine-doped nanocarbon-based(CF_x)materials under different fluorine sources and the function mechanism of CF_x in various batteries are reviewed in detail.Subsequently,judging from the dependence between the structure and electrochemical performance of nanocarbon sources,the progress of CF_x based on different dimensions(0D–3D)for primary battery applications is reviewed and the balance between energy density and power density is critically discussed.In addition,the roles of CF_x materials in secondary batteries and their current applications in recent years are summarized in detail to illustrate the effect of introducing F atoms.Finally,we envisage the prospect of CF_x materials and offer some insights and recommendations to facilitate the further exploration of CF_x materials for various high-performance battery applications.

Introduction to the Special Issue on Advances in Neutrosophic and Plithogenic Sets for Engineering and Sciences:Theory,Models,and Applications

Recently,research on uncertainty modeling has been progressing rapidly,and many essential and breakthrough studies have already been done.There are various ways to handle these uncertainties,such as fuzzy and intuitionistic fuzzy sets.Although these concepts can take incomplete information in various real-world issues,they cannot address all types of uncertainty,such as indeterminate and inconsistent information.The neutrosophic theory founded by Florentin Smarandache in 1998 constitutes a further generalization of fuzzy set,intuitionistic fuzzy set,picture fuzzy set,Pythagorean fuzzy set,spherical fuzzy set,etc.Since then,this logic has been applied in various science and engineering domains.

Rural Revitalization Planning under the Territorial Spatial Planning System

With the rapid development of China’s social economy,village planning is increasingly important.Departments should fully draw up practical village plan“integrating various plans”.In the context of“rural revitalization”,how to better promote“rural revitalization”and realize the concept that“lucid waters and lush mountains are invaluable assets”is a difficult problem in front of everyone.After many years of pilot of“integrating various plans”,“five levels,three categories and four systems”have been formed,but the territorial spatial planning in the new era under the construction of ecological civilization still faces many problems and challenges.In this paper,the practical village planning of“integrating various plans”under the background of territorial spatial planning was analyzed,aiming to establish and improve a complete rural spatial system and provide an effective scheme for rural governance mechanism by analyzing and refining the key points of rural planning.

洛马公司的无人机

重点介绍了最近洛马公司披露的其臭鼬工厂秘密研制的无人机,包括臭鼬、VARIOUS、猎手-杀手、无人型F-35以及猎鹰无人轰炸机,并简单回顾了洛马公司其它研制单位在无人机领域的研究成果。

Kinematic Analysis and Experimental Verification on the Locomotion of Gecko

This paper presents a kinematic analysis of the locomotion of a gecko,and experimental verification of the kinematic model.Kinematic analysis is important for parameter design,dynamic analysis,and optimization in biomimetic robot research. The proposed kinematic analysis can simulate,without iteration,the locomotion of gecko satisfying the constraint conditions that maintain the position of the contacted feet on the surface.So the method has an advantage for analyzing the climbing motion of the quadruped mechanism in a real time application.The kinematic model of a gecko consists of four legs based on 7-degrees of freedom spherical-revolute-spherical joints and two revolute joints in the waist.The motion of the kinematic model is simulated based on measurement data of each joint.The motion of the kinematic model simulates the investigated real gecko's motion by using the experimental results.The analysis solves the forward kinematics by considering the model as a combination of closed and open serial mechanisms under the condition that maintains the contact positions of the attached feet on the ground. The motions of each joint are validated by comparing with the experimental results.In addition to the measured gait,three other gaits are simulated based on the kinematic model.The maximum strides of each gait are calculated by workspace analysis.The result can be used in biomimetic robot design and motion planning.

The Evolution of Organic Carbon in Chinese Mollisol Under Different Farming Systems: Validation and Prediction by Using Century Model

Soil organic carbon （SOC） is an important indicator of soil degradation process. In this study, the long-term SOC evolution in Chinese mollisol farmland was simulated and predicted by validating, analyzing, processing and assorting concerning data, based on clarifying parameters of Century model need, combined with best use of recorded data of field management, observed data of long-term experiments, climate, soil, and biology, and achieved results from Hailun Agro-Ecological Experimental Station, Chinese Academy of Sciences. The results were showed as follows： Before reclamation, SOC content was around 58.00 g kg^-1, SOC content dropped quickly in early years, and then decreased slowly after reclamation. SOC content was around 34.00 g kg^-1 with a yearly average rate of 8.91‰ decrease before long-term experiments was established. After a long-term experiment, SOC would change under different farming systems. Shift farming system changed as follows： By 20-year model simulation, SOC content decreased from 34.03 to 30.19 g kg^-1, with a yearly average rate of 5.97‰; by 100-year model simulation, SOC content decreased to 24.31 g kg^-1, with a yearly average rate of 3.36‰. Organic farming system changed as follows： By 20-year model simulation, SOC content decreased slowly from 34.03 to 33.39 g kg^-1, with a yearly average rate of 0.95‰, 5‰ less than that of shift farming system; by 100-year model simulation, SOC content decreased to 32.21 g kg^-1, with a yearly average rate of 0.55‰. ＂Petroleum＂ farming system changed as follows： By 20-year model simulation, SOC content decreased from 34.03 to 32.88 g kg^-1, with a yearly average rate of 1.72‰, much more than that of organic farming system; by 100-year model simulation, SOC content decreased to 30.89 g kg^-1, with a yearly average rate of 0.96‰. Combined ＂petroleum＂-organic farming system changed as follows： By 20-year model simulation, SOC content was increased slightly; by 100-year model simulation, SOC content increased from 34.03 to 34.41g kg^-1, with a yearly average rate of 0.11‰. The above results provided an optimal way for maintaining SOC in Chinese mollisol farmland： To increase, as much as possible within agro-ecosystem, soil organic matter returns such as crop stubble, crop litter, crop straw or stalk, and manure, besides applying chemical nitrogen and phosphorous, which increased system productivity and maintained SOC content as well. Also, the results provided a valuable methodology both for a study of CO2 sequestration capacity and for a target fertility determination in Chinese mollisol.

Study of total phenol,flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris

Objective:To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol,flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar,Nizwa.Sultanate of Oman.Methods:The leave sample was extracted with methanol and evaporated.Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities.The prepare hexane,chloroform,ethyl acetate,hutanol and methanol crude extracts were used for their evaluation of total phenol,flavonoids contents and phytochemical screening study.The established conventional metliods were used for quantitative determination of total phenol,flavonoids contents and phytochemical screening.Results:Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids,saponins and steroids compounds.The result for total phenol content was the highest in hutanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.Conclusions:The crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.

Fabrication of High-Density Out-of-Plane Microneedle Arrays with Various Heights and Diverse Cross-Sectional Shapes

Out-of-plane microneedle structures are widely used in various applications such as transcutaneous drug delivery and neural signal recording for brain machine interface.This work presents a novel but simple method to fabricate high-density silicon(Si)microneedle arrays with various heights and diverse cross-sectional shapes depending on photomask pattern designs.The proposed fabrication method is composed of a single photolithography and two subsequent deep reactive ion etching(DRIE)steps.First,a photoresist layer was patterned on a Si substrate to define areas to be etched,which will eventually determine the final location and shape of each individual microneedle.Then,the 1st DRIE step created deep trenches with a highly anisotropic etching of the Si substrate.Subsequently,the photoresist was removed for more isotropic etching;the 2nd DRIE isolated and sharpened microneedles from the predefined trench structures.Depending on diverse photomask designs,the 2nd DRIE formed arrays of microneedles that have various height distributions,as well as diverse cross-sectional shapes across the substrate.With these simple steps,high-aspect ratio microneedles were created in the high density of up to 625 microneedles mm^(-2)on a Si wafer.Insertion tests showed a small force as low as~172μN/microneedle is required for microneedle arrays to penetrate the dura mater of a mouse brain.To demonstrate a feasibility of drug delivery application,we also implemented silk microneedle arrays using molding processes.The fabrication method of the present study is expected to be broadly applicable to create microneedle structures for drug delivery,neuroprosthetic devices,and so on.

Uptake,Depuration and Subcellular Distribution of Cadmium in Various Tissues of Perna viridis

Green-lipped mussels (Perna viridis) were collected from a local mariculture site and placed in pre-cleaned sea water tanks containing 0, 0.2, 0.5 and 1.3 μg/ml CdCl2. The level of Cd in the gill, viscera and gonad was measured. The concentration of Cd in the 3 tissues increased linearly over 4 days' of exposure. The rate of uptake depends on the concentration of Cd in the water. In the 3 tissues studied, the maximum rate of uptake (Vmax) was highest in the gill (500μg/g dry weight/day), followed by viscera (100μg/g dry weight/day) and gonad (56.8 μg/g dry weight/day). The majority of the Cd taken up was bound to a fraction of the heat-stable proteins similar to metallothioneins. The rate of Cd depuration from the tissues was poor. There was no change in Cd concentration over 8 days' depuration in clean water

Cell signaling associated with internalization of 67 kDa laminin receptor (67LR) by soluble laminin and its implication for protection against neurodegenerative diseases

Various isoforms of laminins are present in the basement membranes complexed with other structural proteins.In the central nervous system(CNS),they are primarily localized to the basement membranes of blood vessels and reactive astrocytes.Limited amounts of various laminin isoforms are also associated with neuronal cell bodies and axons,which are referred to as“neuronal”laminins(Yamamoto et al.,1988).They exist in a soluble form,free from other basement membrane proteins.Neurons either produce these soluble laminins or acquire them from astroglial cells.Laminin stimulates neuritogenesis and confers neuroprotection in vitro,but their exact role of these neuronal soluble laminins in the CNS is yet unknown.

Chemical vapor deposition growth behavior of graphene

The optimized growth parameters of graphene with different morphologies,such as dendrites,rectangle,and hexagon,have been obtained by low-pressure chemical vapor deposition on polycrystalline copper substrates.The evolution of fractal graphene,which grew on the polycrystalline copper substrate,has also been observed.When the equilibrium growth state of graphene is disrupted,its intrinsic hexagonal symmetry structure will change into a non-hexagonal symmetry structure.Then,we present a systematic and comprehensive study of the evolution of graphene with different morphologies grown on solid copper as a function of the volume ratio of methane to hydrogen in a controllable manner.Moreover,the phenomena of stitching snow-like graphene together and stacking graphene with different angles was also observed.

Study on the Singlet and Triplet StateElectron Transfer between Hypocrellin A and Aromatic Amines in Solvents of Various Polarity

In this paper, the singlet and triplet state electron transfer processes between hypocrellin A(HA) and aromatic amines in solvents of various polarity have been studied by fluorescence and time-resolved transient absorption spectra. The results show,in.polar solvent, the quenching of HA fluorescence by aromatic amines results in Stern-Volmer plot, the investigation of transient absorption indicated that triplet-state electron transfer process from 3HA to the ground-state of amines occured, and the wines of the singlet and triplet state electron transfer rate constant were calculated, however in weak polar solvent, only the triplet-triplet absorption of HA was observed.

Mechanical properties evaluation for thin film/substrate material systems

Thin film/substrate material systems have been widely used in various industries such as aerospace,automotive,marine and electronics.One example is thermal barrier coatings(TBCs):TBCs are extensively applied to metallic components on gas turbine engines in order to protect them from high and prolonged heat loads,and they play an essential role in the aircraft industry.Another example is laminated polymer matrix composites(PMCs),reinforced with fibers such as carbon,glass or aramid:These materials have been extensively employed in a variety of applications due to their superior properties and the improving ease and cost-effectiveness of fabricating them.The evaluation of mechanical properties for thin film/substrate material systems is imperative for more efficient use of these material systems and further improvements.

A Controllable Synthetic Route for Preparing Graphene-Cu and Graphene-Cu_2O Nanocomposites Using Graphene Oxide-CuO as a Precursor

The development of convenient method to obtain graphene-based nanocomposites is a key issue for their application. Herein, we described a facile route for synthesizing graphene-Cu and graphene-Cu2 O nanocomposites using graphene oxide-Cu O as a precursor. Remarkably, the different nanocomposites could be formed just by varying the reaction temperature and time. This work provides a feasible route for the preparation of graphene-based nanocomposites with various constituents.

Vibrating-Sliding Motion of Caisson Breakwaters Under Various Breaking Wave Impact Forces

Sliding is one of the principal failure types of caisson breakwaters and is an essential content of stability examination in caisson breakwater design. Herein, the mass-spring-dashpot model of caisson-base system is used to simulate the vibrating-sliding motion of the caisson under various types of breaking wave impact forces, i.e., single peak impact force, double peak impact force, and shock-damping oscillation impact force. The effects of various breaking wave impacts and the sliding motion on the dynamic response behaviors of caisson breakwaters are investigated and the calculation of relevant system parameters is discussed. It is shown that the dynamic responses of the caisson are significantly different under different types of breaking wave impact forces even when the amplitudes of impact forces are equal. The amplitude of dynamic response of the caisson is lower under single peak impact excitation than that under double peak impact or shock-damping oscillation impact excitation. Though the displacement of the caisson is large due to sliding, the rotation, the sliding force and the overturning moment of the caisson are significantly reduced.

A review on electronically conducting polymers for lithium-sulfur battery and lithium-selenium battery:Progress and prospects

Lithium-sulfur(Li-S) batteries and lithium-selenium(Li-Se) batteries,as environmental protection energy storage systems with outstanding theoretical specific capacities and high energy densities,have become the hotspots of current researches.Besides,elemental S(Se) raw materials are widely sourced and their production costs are both low,which make them considered one of the new generations of high energy density electrochemical energy storage systems with the most potential for development.However,poor conductivity of elemental S/Se and the notorious "shuttle effect" of lithium polysulfides(polyselenides) severely hinder the commercialization of Li-S/Se batteries.Thanks to the excellent electrical conductivity and strong absorption of lithium polysulfide(polyselenide) about electronically conducting polymer,some of the above thorny problems have been effectively alleviated.The review presents the fundamental studies and current development trends of common electronically conducting polymers in various components of Li-S/Se batteries,which involves polyaniline(PANI) polypyrrole(PPy),and polythiophene(PTh) with its derivatives,e.g.polyethoxythiophene(PEDOT) and poly(3,4-ethylene dioxythiophene)-poly(styrenesulfonate)(PEDOT:PSS).Finally,the review not only summarizes the research directions and challenges facing the application of electronically conducting polymers,but also looks forward to the development prospects of them,which will provide a way for the practical use of electronically conducting polymers in Li-S/Se batteries with outstanding electrochemical properties in the short run.