Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external ca...Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external carotid arteries of New Zealand rabbits. Following model induction, lentivirus carrying basic fibroblast growth factor was injected through the ear vein. We found that the longer the action time of the lentivirus, the smaller the aneurysm volume. Moreover, platelet-derived growth factor expression in the aneurysm increased, but smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression decreased. At 1,2, 3, and 4 weeks following model establishment, following 1 week of injection of lentivirus carrying basic fibroblast growth factor, the later the intervention time, the more severe the blood vessel damage, and the bigger the aneurysm volume, the lower the smooth muscle 22 aJpha and hypertension-related gene ~ mRNA expression. Simultaneously, platelet-derived growth factor expression decreased. These data suggest that recombinant lentivirus carrying basic fibroblast growth factor can repair damaged cells in the aneurysmal wall and inhibit aneurysm dynamic growth, and that the effect is dependent on therapeutic duration.展开更多
探讨SM-22α、α-SMA水平变化与下肢静脉曲张的相关性及其意义。采用间苯二酚品红染色法观察血管平滑肌细胞（VSMCs）结构,通过免疫组织化学染色观察正常大隐静脉及曲张大隐静脉切片SM-22α和α-SMA表达情况;利用RT-PCR检测正常大隐静...探讨SM-22α、α-SMA水平变化与下肢静脉曲张的相关性及其意义。采用间苯二酚品红染色法观察血管平滑肌细胞（VSMCs）结构,通过免疫组织化学染色观察正常大隐静脉及曲张大隐静脉切片SM-22α和α-SMA表达情况;利用RT-PCR检测正常大隐静脉及曲张大隐静脉中SM-22α和α-SMA m RNA表达水平,并用Western blotting检测正常大隐静脉及曲张大隐静脉SM-22α和α-SMA蛋白表达水平。结果显示,曲张静脉管壁血管平滑肌细胞SM-22α和α-SMA阳性表达率较正常大隐静脉均明显降低,与正常组比较,静脉曲张患者血清中SM-22α和α-SMA m RNA表达水平均明显降低,血管管壁平滑肌细胞SM-22α和α-SMA蛋白表达水平均明显降低（p〈0.05或p〈0.01）。曲张静脉VSMCs有明显向内膜增殖“迁移”现象,且收缩型VSMCs中SM-22α和α-SMA m RNA及蛋白表达水平均随VSMCs去分化程度增加而减少,这可能是静脉曲张发生、发展的机制之一。展开更多
基金funded by the Key Medical SubjectProject of Jiangsu Province, No. XK2007227
文摘Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external carotid arteries of New Zealand rabbits. Following model induction, lentivirus carrying basic fibroblast growth factor was injected through the ear vein. We found that the longer the action time of the lentivirus, the smaller the aneurysm volume. Moreover, platelet-derived growth factor expression in the aneurysm increased, but smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression decreased. At 1,2, 3, and 4 weeks following model establishment, following 1 week of injection of lentivirus carrying basic fibroblast growth factor, the later the intervention time, the more severe the blood vessel damage, and the bigger the aneurysm volume, the lower the smooth muscle 22 aJpha and hypertension-related gene ~ mRNA expression. Simultaneously, platelet-derived growth factor expression decreased. These data suggest that recombinant lentivirus carrying basic fibroblast growth factor can repair damaged cells in the aneurysmal wall and inhibit aneurysm dynamic growth, and that the effect is dependent on therapeutic duration.
文摘探讨SM-22α、α-SMA水平变化与下肢静脉曲张的相关性及其意义。采用间苯二酚品红染色法观察血管平滑肌细胞（VSMCs）结构,通过免疫组织化学染色观察正常大隐静脉及曲张大隐静脉切片SM-22α和α-SMA表达情况;利用RT-PCR检测正常大隐静脉及曲张大隐静脉中SM-22α和α-SMA m RNA表达水平,并用Western blotting检测正常大隐静脉及曲张大隐静脉SM-22α和α-SMA蛋白表达水平。结果显示,曲张静脉管壁血管平滑肌细胞SM-22α和α-SMA阳性表达率较正常大隐静脉均明显降低,与正常组比较,静脉曲张患者血清中SM-22α和α-SMA m RNA表达水平均明显降低,血管管壁平滑肌细胞SM-22α和α-SMA蛋白表达水平均明显降低（p〈0.05或p〈0.01）。曲张静脉VSMCs有明显向内膜增殖“迁移”现象,且收缩型VSMCs中SM-22α和α-SMA m RNA及蛋白表达水平均随VSMCs去分化程度增加而减少,这可能是静脉曲张发生、发展的机制之一。
