Objective To give a concise review of the current state of the art in tissue engineering （TE） related to skeletal muscle and kinds of bioreactor environment.Data sources The review was based on data obtained from th...Objective To give a concise review of the current state of the art in tissue engineering （TE） related to skeletal muscle and kinds of bioreactor environment.Data sources The review was based on data obtained from the published articles and guidelines.Study selection A total of 106 articles were selected from several hundred original articles or reviews.The content of selected articles is in accordance with our purpose and the authors are authorized scientists in the study of engineered muscle tissue in bioreactor.Results Skeletal muscle TE is a promising interdisciplinary field which aims at the reconstruction of skeletal muscle loss.Although numerous studies have indicated that engineering skeletal muscle tissue may be of great importance in medicine in the near future,this technique still represents a limited degree of success.Since tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen,carbon dioxide,nutrients and waste products.Moreover,functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural calls.Third,in order to engineer muscle tissue successfully,it may be beneficial to mimic the in vivo environment of muscle through association with adequate stimuli from bioreactors.Conclusion Vascular system and bioreactors are necessary for development and maintenance of engineered muscle in order to provide circulation within the construct.展开更多
目的对用于骨组织工程的体内生物反应器(in vivo bioreactor,IVB)的研究进展进行综述,为后续研究提供参考。方法查阅近年来骨组织工程IVB相关文献,从IVB构建原则、构建IVB的组织类型、部位及方法,以及IVB用于构建组织工程骨的优劣等方...目的对用于骨组织工程的体内生物反应器(in vivo bioreactor,IVB)的研究进展进行综述,为后续研究提供参考。方法查阅近年来骨组织工程IVB相关文献,从IVB构建原则、构建IVB的组织类型、部位及方法,以及IVB用于构建组织工程骨的优劣等方面进行综述。结果 IVB是利用机体自我再生能力,把身体作为生物反应器在损伤部位或能够支持新组织再生的异位部位再生新组织或器官。IVB可由组织瓣(皮下袋、肌瓣/袋、筋膜瓣、骨膜瓣、大网膜瓣/腹腔)和轴向血管蒂(轴向血管束、动静脉环)单独或联合构建。使用IVB能预构与骨缺损形状、大小匹配的血管化组织工程骨。预构的骨组织可作为游离骨移植物、带蒂骨瓣或游离骨瓣修复骨缺损。IVB解决了传统骨组织工程血管化不足的问题。结论 IVB是一种有临床应用前景的血管化组织工程骨预构和骨缺损修复重建的方法,在修复大型复杂骨缺损方面有着独特优势。但因IVB存在构建复杂以及手术并发症等问题影响了其临床应用,后续需致力于开发一种简单、安全、高效的IVB。展开更多
文摘Objective To give a concise review of the current state of the art in tissue engineering （TE） related to skeletal muscle and kinds of bioreactor environment.Data sources The review was based on data obtained from the published articles and guidelines.Study selection A total of 106 articles were selected from several hundred original articles or reviews.The content of selected articles is in accordance with our purpose and the authors are authorized scientists in the study of engineered muscle tissue in bioreactor.Results Skeletal muscle TE is a promising interdisciplinary field which aims at the reconstruction of skeletal muscle loss.Although numerous studies have indicated that engineering skeletal muscle tissue may be of great importance in medicine in the near future,this technique still represents a limited degree of success.Since tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen,carbon dioxide,nutrients and waste products.Moreover,functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural calls.Third,in order to engineer muscle tissue successfully,it may be beneficial to mimic the in vivo environment of muscle through association with adequate stimuli from bioreactors.Conclusion Vascular system and bioreactors are necessary for development and maintenance of engineered muscle in order to provide circulation within the construct.
文摘目的对用于骨组织工程的体内生物反应器(in vivo bioreactor,IVB)的研究进展进行综述,为后续研究提供参考。方法查阅近年来骨组织工程IVB相关文献,从IVB构建原则、构建IVB的组织类型、部位及方法,以及IVB用于构建组织工程骨的优劣等方面进行综述。结果 IVB是利用机体自我再生能力,把身体作为生物反应器在损伤部位或能够支持新组织再生的异位部位再生新组织或器官。IVB可由组织瓣(皮下袋、肌瓣/袋、筋膜瓣、骨膜瓣、大网膜瓣/腹腔)和轴向血管蒂(轴向血管束、动静脉环)单独或联合构建。使用IVB能预构与骨缺损形状、大小匹配的血管化组织工程骨。预构的骨组织可作为游离骨移植物、带蒂骨瓣或游离骨瓣修复骨缺损。IVB解决了传统骨组织工程血管化不足的问题。结论 IVB是一种有临床应用前景的血管化组织工程骨预构和骨缺损修复重建的方法,在修复大型复杂骨缺损方面有着独特优势。但因IVB存在构建复杂以及手术并发症等问题影响了其临床应用,后续需致力于开发一种简单、安全、高效的IVB。
