期刊文献+
共找到1,159篇文章
< 1 2 58 >
每页显示 20 50 100
Serum Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Level in Patients with Colorectal Carcinoma and Clinical Significance 被引量:16
1
作者 许天文 陈道达 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第3期329-331,355,共4页
Circulating vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor (VEGF) levels in patients with colorectal carcinoma were determined in order to assess their clinical significance a... Circulating vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor (VEGF) levels in patients with colorectal carcinoma were determined in order to assess their clinical significance as a diagnostic tool for monitoring lymph node metastasis. In 66 patients with colorectal carcinoma and 30 healthy controls, circulating VEGF-C and VEGF levels were assessed by using enzyme-linked immunosorbent assay (ELISA). Serum VEGF-C and VEGF levels were higher in patients with colorectal carcinoma than in healthy controls. Patients with lymph node metastasis had higher serum VEGF-C and VEGF levels than those without lymph node metastasis. The levels of VEGF-C and VEGF were higher in the invasion group than in the non-invasion group. Serum VEGF-C levels reached a sensitivity of 81% and a specificity of 76 % with a cutoff value of 1438.0 pg/mL, whereas VEGF levels reached 72 % sensitivity and 74 % specificity at 240.2 pg/ mL. If 66 patients were divided into 4 groups according to the combined determination of VEGF-C and VEGF levels, the positive predictive value was 85.3 %, the negative predictive value was 94.6 %, and accuracy was 93.7 %. It was suggested that circulating VEGF-C levels might provide additional information for distinguishing the absence from presence of lymph node metastasis in patients with colorectal carcinoma. The combined determination of VEGF-C and VEGF levels could be used as an important index for preoperatively clinical stage of colorectal carcinoma. 展开更多
关键词 colorectal carcinoma vascular endothelial growth factor c vascular endothelial growth factor lymph node metastasis enzyme-linked immunosorbent assay
下载PDF
Expression of Vascular Endothelial Growth Factor C and Its Correlation with Lymph Node Metastasis in Colorectal Carcinoma 被引量:9
2
作者 许天文 陈道达 陈剑英 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第6期596-598,共3页
Summary: To study the expression of vascular endothelial growth factor C (VEGF-C) in colorectal carcinoma and its relationship with lymph node metastasis, the expression of VEGF-C protein in colorectal carcinoma tissu... Summary: To study the expression of vascular endothelial growth factor C (VEGF-C) in colorectal carcinoma and its relationship with lymph node metastasis, the expression of VEGF-C protein in colorectal carcinoma tissues obtained from 94 patients who underwent radical resection was immunohistochemically detected. Meanwhile, the expression of VEGF-C mRNA in 4 colorectal carcinoma cell lines was examined by reverse transcription polymerase chain reaction (RT-PCR).VEGF-C protein was found to be expressed in 53.2 % of patients. The expression was more frequently detected in tumors with lymph node metastasis than in those without metastasis (P<0.01), and there was significant correlation between its expression and lymphatic invasion, TNM stage (P<0.01). However, no significant correlation was found between its expression and the age, gender, tumor location, depth of invasion and vascular invasion. 2 of the 4 colorectal carcinoma cell lines, including LoVo and LoVo-5FU, expressed VEGF-C mRNA. The expression of VEGF-C is closely related to lymph node metastasis, and it might take part in the tumor lymphangiogenesis. 展开更多
关键词 colorectal carcinoma vascular endothelial growth factor c lymph node METASTASIS LYMPHANGIOGENESIS
下载PDF
Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer 被引量:6
3
作者 Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2005年第3期460-463,共4页
Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines a... Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. 展开更多
关键词 pancreatic cancer vascular endothelial growth factor vegf-c cYTOKINE INTERLEUKIN-1Α INTERLEUKIN-6
下载PDF
Effect of Antisense Oligodeoxynucleotide of Vascular Endothelial Growth Factor C on Lymphangiogenesis and Angiogenesis of Pancreatic Cancer 被引量:3
4
作者 李凯 陶京 +5 位作者 李弢 许州 杨智勇 吴河水 熊炯炘 王春友 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第1期51-53,共3页
In order to investigate the effect of antisense oligonucleotide (ASODN) of vascular endothelial growth factor C (VEGF-C) on lymphangiogenesis and angiogenesis of pancreatic cancer, antisense and scamble-sense olig... In order to investigate the effect of antisense oligonucleotide (ASODN) of vascular endothelial growth factor C (VEGF-C) on lymphangiogenesis and angiogenesis of pancreatic cancer, antisense and scamble-sense oligonucleotide of VEGF-C were constructed, and the model of nude mice with orthotopically xenografied human pancreatic cancer cells (Panc-1) was established. Thirty nude mice were randomly divided into 3 groups: PBS control group (group A), scramble-sense control group (group B) and antisense group (group C). All nude mice were treated once every 2 days as 3 times per week, for 3 weeks (oligonucleotide 10 mg/kg every time). After treatments were completed, ELISA method was used to examine the concentration of VEGF-C in plasma and immunohistochemical method to examine microvessel density (MVD), lymphtic vessel density (LVD) of pancreatic cancer. The results showed that the expression of VEGF-C was inhibited significantly in group C. The concentrations were 237.5±41.5, 221.5±52.3 and 108.6±14.9 pg/mL in groups A, B and C respectively (P〈0.01). LVD in groups A, B and C was 13.8±2.1, 12.4±1.9 and 4.2±1.6 respectively (P〈0.01). MVD in groups A, B and C was 27.5±8.7, 25.9±4.2 and 19.4±5.6 respectively with no significant difference among the groups (P〉0.05). It was suggested that VEGF-C ASODN decreased the expression levels of VEGF-C in nude mice with orthotopically xenografted human pancreatic cancer, and it could inhibit lymphangiogenesis, but had no significant effect on angiogenesis. 展开更多
关键词 pancreatic cancer vascular endothelial growth factor c LYMPHANGIOGENESIS ANGIOGENESIS .gene therapy
下载PDF
Expression of vascular endothelial growth factors A and C in human pancreatic cancer 被引量:5
5
作者 Rui-Feng Tang Shu-Xia Wang +6 位作者 Li Peng Shun-Xiang Wang Meng Zhang Zhi-Feng Li Zhi-Ming Zhang Yan Xiao Feng-Rui Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第2期280-286,共7页
瞄准:学习脉管的内皮生长因素 A 的表示(VEGF -- 一) 并且 VEGF-C 并且决定是否 VEGF 的存在 -- A 和 VEGF-C 与胰腺的癌症的临床病理特征被联系。方法:VEGF -- A 和 VEGF-C mRNA 抄本被北污点在 6 根人的胰腺的癌症房间线和 8 正常... 瞄准:学习脉管的内皮生长因素 A 的表示(VEGF -- 一) 并且 VEGF-C 并且决定是否 VEGF 的存在 -- A 和 VEGF-C 与胰腺的癌症的临床病理特征被联系。方法:VEGF -- A 和 VEGF-C mRNA 抄本被北污点在 6 根人的胰腺的癌症房间线和 8 正常胰腺的纸巾和 8 个胰腺的癌标本检验。VEGF 的表示 -- A 和 VEGF-C 蛋白质被西方的污点在测试房间线检验,由免疫,在 50 胰腺的癌的组织化学的污点取样。结果:VEGF -- A 和 VEGF-C mRNA 抄本在所有 6 根人的胰腺的癌症房间线是在场的。Immunoblotting 揭示了 VEGF 的存在 -- 在所有房间的 A 和 VEGF-C 蛋白质排队。全部的 RNA 的北污点分析在 VEGF 揭示了 3.0 褶层和 3.6 褶层增加 -- 在癌症的 A 和 VEGF-C mRNA 抄本分别地取样。Immunohistochemical 分析证实了 VEGF 的表示 -- 在在肿瘤以内的癌症房间的 A 和 VEGF-C 集中。50 件胰腺的癌症织物样品的 Immunohistochemical 分析揭示了 VEGF 的存在 -- 在 50% 癌症织物和 80% 的 A 和 VEGF-C 免疫反应分别地取样。VEGF 的存在 -- 在这些房间的 A 与更大的肿瘤尺寸被联系并且提高了本地传播(c2 = 6.690, P = 0.035 【0.05 ) 但是没与减少的耐心的幸存被联系。然而,在癌症房间的 VEGF-C 的存在与增加的淋巴节点转移被联系(c2 = 5.710, P = 0.017 【 0.05 ) ,但是没与减少的耐心的幸存被联系。在 VEGF 的表示之间没有关联 -- 在一样的癌症房间的 A 和 VEGF-C。结论:VEGF -- A 和 VEGF-C 是通常在上在人的胰腺的癌症表示了并且可以贡献肿瘤生长和淋巴节点转移。在 VEGF 的表示之间没有关系 -- 在胰腺的癌症的 A 和 VEGF-C。 展开更多
关键词 胰腺癌 血管内皮生长因子A 血管内皮生长因子c 成活率
下载PDF
STI571 (Glivec) suppresses the expression of vascular endothelial growth factor in the gastrointestinal stromal tumor cell line,GIST-T1 被引量:14
6
作者 Toufeng Jin Hajime Nakatani +5 位作者 Takahiro Taguchi Takumi Nakano Takehiro Okabayashi Takeki Sugimoto Michiya Kobayashi Keijiro Araki 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第5期703-708,共6页
AIM:To estimate whether STI571 inhibits the expressionof vascular endothelial growth factor(VEGF)in thegastrointestinal stromal tumor(GIST)cells.METHODS:We used GIST cell line,GIST-T1.It hasa heterogenic 57-bp deletio... AIM:To estimate whether STI571 inhibits the expressionof vascular endothelial growth factor(VEGF)in thegastrointestinal stromal tumor(GIST)cells.METHODS:We used GIST cell line,GIST-T1.It hasa heterogenic 57-bp deletion in exon 11 to produce amutated c-KIT,which results in constitutive activationof c-KIT.Cells were treated with/without STI571 orstem cell factor(SCF).Transcription and expression ofVEGF were determined by RT-PCR and flow cytometryor Western blotting,respectively.Activated c-KIT wasestimated by immunoprecipitation analysis.Cell viabilitywas determined by MTT assay.RESULTS:Activation of c-KIT was inhibited bySTI571 treatment.VEGF was suppressed at both thetranscriptional and translational levels in a temporal anddose-dependent manner by STI571.SCF upregulatedthe expression of VEGF and it was inhibited by STI571.STI571 also reduced the cell viability of the GIST-T1cells,as determined by MTT assay.CONCLUSION:Activation of c-KIT in the GIST-T1regulated the expression of VEGF and it was inhibited bySTI571.STI571 has antitumor effects on the GIST cellswith respect to not only the inhibition of cell growth,butalso the suppression of VEGF expression. 展开更多
关键词 内皮生长因子 基因表达 ST1571 胃肠肿瘤 肿瘤血管
下载PDF
Expression of vascular endothelial growth factor-C and angiogenesis in esophageal squamous cell carcinoma 被引量:16
7
作者 Ming-Xing Ding Xing-Qiu Lin +2 位作者 Xiao-Yan Fu Ning Zhang Ji-Cheng Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第28期4582-4585,共4页
瞄准:调查脉管的内皮生长 factor-c (VEGF-C ) 的表示在人的食道的有鳞的房间癌(ESCC ) 和它与临床的意义的关系的 mRNA 和微容器密度(MVD ) 。方法:从为 ESCC 经历外科的切除术的 43 个病人获得的标本在这研究被使用。VEGF-C mRNA ... 瞄准:调查脉管的内皮生长 factor-c (VEGF-C ) 的表示在人的食道的有鳞的房间癌(ESCC ) 和它与临床的意义的关系的 mRNA 和微容器密度(MVD ) 。方法:从为 ESCC 经历外科的切除术的 43 个病人获得的标本在这研究被使用。VEGF-C mRNA 的表示被原位杂交检验。肿瘤 MVD 与 anti-CD31 抗体组织化学地是坚定的免疫并且由图象分析估计了。邻近的正常粘膜的十节也被检验。结果:VEGF-C mRNA 表示在癌房间的细胞质被检测。病人们学习了的 43 ESCC, 18 个案例(41.9%) 为 VEGF-C mRNA 是积极的。没有 VEGF-C mRNA 表示在正常食道的粘膜被观察。VEGF-C mRNA 表示与淋巴节点转移, TNM 阶段和侵略的深度显著地相关(P 【 0.05 ) 。而且,组织学的等级(区别) 趋于与 VEGF-C mRNA 表示相关,但是不是统计上重要的(P 】 0.05 ) 。在肿瘤损害, MVD 在正常食道的粘膜比那显著地大。MVD 与淋巴节点转移, TNM 阶段和侵略的深度显著地相关(P 【 0.05 ) ,然而并非与组织学的等级(区别)(P 】 0.05 ) 。没有 VEGF-C mRNA 表示,有 VEGF-C mRNA 表示的损害比那些的有显著地更高的 MVD (P 【 0.05 ) 。结论:VEGF-C 在 ESCC 经由 lymphangiogenesis 和血管生成在淋巴的转移起一个作用。VEGF-C 是在 ESCC 的生物行为的重要预言者之一。 展开更多
关键词 血管内皮生长因子-c 血管生成 食管鳞状细胞细胞 治疗
下载PDF
Effect of c-fos antisense probe on prostaglandin E_2-induced upregulation of vascular endothelial growth factor mRNA in human liver cancer cells 被引量:5
8
作者 Yong-Qi Li Ning Ren +1 位作者 Yi-Hu Wang Kai-Shan Tao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第28期4427-4430,共4页
AIM: To examine the effect of prostaglandin E2 (PGE2) on the expression of vascular endothelial growth factor (VEGF) mRNA in the human hepatocellular carcinoma (HCC) HepG2 cells and the possible involvement of c-fos p... AIM: To examine the effect of prostaglandin E2 (PGE2) on the expression of vascular endothelial growth factor (VEGF) mRNA in the human hepatocellular carcinoma (HCC) HepG2 cells and the possible involvement of c-fos protein in this process.METHODS: Human HCC HepG2 cells were divided into three groups treated respectively with PGE2, a combination of PGE2 and c-fos antisense oligodeoxynucleotide (ASO),and PGE2 plus c-fos sense oligodeoxynudeotide (SO). The expression of VEGF mRNA in HepG2 cells after different treatments was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The relative expression level of VEGF mRNA in HepG2 cells in each group was measured.RESULTS: Administration of PGE2 resulted in an increased expression of c-fosand VEGF mRNA in HepG2 cells. The relative expression level of c-fos mRNA reached the peak at 3 h (68.4±4.7%) after PGE2 treatment, which was significantly higher than that at 0 h (20.6±1.7%, P<0.01).Whereas, the highest expression level of VEGF mRNA was observed at 6 h (100.5±6.1%) after PGE2 treatment, which was significantly higher than that at 0 h (33.2±2.4%,P<0.01). C-fos ASO significantly reduced PGE2-induced VEGF mRNA expression in HepG2 cells.CONCLUSION: PGE2 increases the expression and secretion of VEGF in HCC cells by activating the transcription factor c-fos, promotes the angiogenesis of HCC and plays an important role in the pathogenesis of liver cancer. 展开更多
关键词 前列腺素E2 血管生长因子 MRNA 肝癌 肿瘤细胞
下载PDF
Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3 in Ovarian Epithelial Tumors 被引量:1
9
作者 傅晓艳 丁明星 +2 位作者 张宁 林兴秋 李继承 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2007年第2期124-130,共7页
Objective: To explore the role of vascular endothelial growth factor-C (VEGF-C) in the process of angiogenesis, lymphangiogenesis and lymphatic metastasis in epithelial ovarian tumors. Methods: In situ hybridizati... Objective: To explore the role of vascular endothelial growth factor-C (VEGF-C) in the process of angiogenesis, lymphangiogenesis and lymphatic metastasis in epithelial ovarian tumors. Methods: In situ hybridization and immunohistochemical staining for VEGF-C were performed in 30 epithelial ovarian carcinomas, 9 borderline tumors and 26 benign tumors. Endothelial cells were immunostained with anti-VEGFR-3 pAb and anti-CD31 mAb, and VEGFR-3 positive vessels and microvessel density (MVD) were assessed by image analysis. Results: VEGF-C mRNA and protein expression were detected in cytoplasm of carcinoma cells. VEGF-C mRNA and protein expression in ovarian epithelial carcinomas were significantly higher than those in borderline tumors and benign tumors (P〈0.05 or P〈0.01). In ovarian epithelial carcinomas, VEGF-C protein expression, VEGFR-3 positive vessels and MVD were significantly higher in the cases of clinical stage Ⅲ-Ⅳ and with lymph node metastasis than those of clinical stage Ⅰ-Ⅱ and without lymph node metastasis respectively (P〈0.05 or P〈0.01). VEGFR-3 positive vessels and MVD were significantly higher in VEGF-C protein positive tumors than negative tumors (P〈0.05). VEGFR-3 positive vessels was significantly correlated with MVD(P〈0.01). Conclusion: VEGF-C might play a role in lymphatic metastasis via lymphangiogenesis and angiogenesis in epithelial ovarian tumors, and VBEGF-C could be used as a biologic marker of metastasis in ovarian epithelial tumors. 展开更多
关键词 Ovarian neoplasms Vascular endothelial growth factor-c (vegf-c VEGF receptor-3(VEGFR-3) cD 31 METASTASIS
下载PDF
Detection of expression of vascular endothelial growth factor C/VEGFR-3 in early stage cervical cancer by tissue microarray assay and its significance 被引量:1
10
作者 SHANG Hai-xia WU Su-hui LI Ying 《山西医科大学学报》 CAS 2009年第9期845-849,共5页
关键词 早期子宫癌 诊断 淋巴结转移 动脉血管
下载PDF
C-reactive protein,procalcitonin,interleukin-6,vascular endothelial growth factor and oxidative metabolites in diagnosis of infection and staging in patients with gastric cancer 被引量:18
11
作者 NevinIlhan NecipIlhan +2 位作者 MehmetKcksu Yavuz Ilhan Handan Akbulut 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第8期1115-1120,共6页
The current study was to determine the serum/plasmalevels of VEGF, IL-6, malondialdehyde (MDA), nitric oxide(NO), PCT and CRP in gastric carcinoma and correlation withthe stages of the disease and accompanying infection.
关键词 c反应蛋白 白细胞介素-6 血管内皮生长因子 氧化代谢物 胃癌 肿瘤标志物
下载PDF
Expressions of vascular endothelial growth factor in cirrhotic tissues and their relations to proto-oncogene c-fos, c-myc
12
作者 Bao-Min Shi Xiu-Yan Wang +4 位作者 Qing-Ling Mu Tai-Huang Wu Zhen Yang Li Zhang Da-Peng Li the Department of General Surgery, Shandong Provincial Hospital, Jinan 250021, China Department of General Surgery, Tongji Hospital, Tongji Medical University, Wuhan 430030, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2002年第3期388-391,共4页
Objective: To investigate the significance of vascular endothelial growth factor (VEGF) in the pathogene- sis of liver cirrhosis and the correlation between VEGF and proto-oncogene c-fos and c-myc in cir- rhotic liver... Objective: To investigate the significance of vascular endothelial growth factor (VEGF) in the pathogene- sis of liver cirrhosis and the correlation between VEGF and proto-oncogene c-fos and c-myc in cir- rhotic liver. Methods: The proteins of VEGF, c-fos, and c-myc were identified immunohistochemically in each tissue section of 53 cases of liver cirrhosis. The correlations between VEGF, c-fos and c-myc were analyzed. The levels of VEGF protein in different Child gradings were also compared. Results: The proteins of VEGF were more highly ex- pressed in Child A and B patients than in Child C patients and controls. The expressions of both c-fos and c-myc were not statistically significant between VEGF positive and negative patients. Conclusions: The protein level of VEGF can reflect the compensation status of cirrhosis patients and may act as an anti-cirrhotic factor. The proto-oncogene c- fos, c-myc and VEGF may have different mecha- nisms in the course of cirrhosis or hepatic tumorigen- esis. 展开更多
关键词 vascular endothelial growth factor liver cirrhosis c-FOS c-MYc child grading
下载PDF
Expression of Vascular Endothelial Growth Factor C and Its Clinical Significance in Human Esophageal Squamous Cell Carcinoma
13
作者 Hongxin Zhang Lan Zhang +3 位作者 Kuisheng Chen Dongling Gao Fucheng He Yunhan Zhang 《Chinese Journal of Clinical Oncology》 CSCD 2007年第2期83-88,共6页
OBJECTIVE To examine the expression of vascular endothelial growth factor C(VEGF-C)in human esophageal squamous cell carcinoma(ESCC), and to clarify its role in lymphatic metastasis in ESCC patients. METHODS Esophagea... OBJECTIVE To examine the expression of vascular endothelial growth factor C(VEGF-C)in human esophageal squamous cell carcinoma(ESCC), and to clarify its role in lymphatic metastasis in ESCC patients. METHODS Esophageal carcinoma EC9706 cel s and samples from 49 patients with primary ESCC were investigated by using S-P immunohisto- chemistry(IHC),the semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)and in situ hybridization(ISH)methods for VEGF-C expression. RESULTS VEGF-C positive expression was found in EC9706 cells through IHC,ISH and RT-PCR.Positive IHC for VEGF-C was observed in 36 of 49 cases of ESCC.There was a significant difference between the expres- sion of VEGF-C in a lymph-node-positive group compared to a node-nega- tive group(χ2=4.7,P<0.05).Positive ISH for VEGF-C mRNA was observed in 23 of 49 cases of ESCC.There was a significant difference between the expression of VEGF-C in the lymph-node-positive group and node-negative group(χ2=31.3,P<0.01).The expression of VEGF-C was significantly higher in the lymph-node-positive group compared to the node-negative group.Of 49 ESCC tissues,RT-PCR for VEGF-C mRNA was observed positively in 29 cases.There was a significant difference between the expression of VEGF-C in the lymph-node-positive group and node-negative group(χ2=23.3, P<0.01).The expression of VEGF-C was significantly higher in the lymph- node-positive group compared to the node-negative group.Expressions of VEGF-C were not significantly associated with age,gender,and pathological grade.There was a relationship between VEGF-C mRNA expressions by RT-PCR and ISH(χ2=18.5,P<0.01)in ESCC cases,but with no significant difference between the two methods. CONCLUSION VEGF-C expression may induce lymphangiogenesis in human ESCC.There was a close correlation between VEGF-C expression and lymph node metastasis.VEGF-C can serve as a useful prognostic factor for ESCC patients. 展开更多
关键词 血管内皮生长因子c 基因表达 临床意义 食管鳞状上皮细胞癌 vegf-c
下载PDF
LINC00958通过上调VEGF-C表达促进宫颈癌细胞的恶性生物学行为及小鼠模型的淋巴结转移
14
作者 靖爽 和晓利 +1 位作者 井佳雨 王悦 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第2期161-168,共8页
目的:探讨LINC00958/血管内皮生长因子C(VEGF-C)信号通路在宫颈癌的淋巴管生成和淋巴转移中的作用。方法:从2020年9月至2022年9月期间在河南省人民医院接受手术的患者中收集了42例宫颈癌组织标本,通过qPCR检测宫颈癌组织和宫颈癌细胞(H... 目的:探讨LINC00958/血管内皮生长因子C(VEGF-C)信号通路在宫颈癌的淋巴管生成和淋巴转移中的作用。方法:从2020年9月至2022年9月期间在河南省人民医院接受手术的患者中收集了42例宫颈癌组织标本,通过qPCR检测宫颈癌组织和宫颈癌细胞(Hela、C33A、SiHa、Caski)中LINC00958的表达情况。将LINC00958过表达载体(LINC00958组)或对照载体(CMV组)转染Caski细胞,敲减LINC00958(shLINC00958组)、VEGF-C(shVEGF-C组)的shRNA序列或阴性对照shRNA(shNC组)转染SiHa细胞。分别通过CCK-8法、Transwell实验检测过表达或敲减LINC00958对宫颈癌细胞增殖、迁移和侵袭的影响。观察转染后细胞的培养上清液对人淋巴管内皮细胞(HLEC)淋巴管形成能力的影响。建立小鼠腘淋巴结转移模型,观察过表达LINC00958或同时敲减VEGF-C对宫颈癌淋巴结转移的影响。结果:LINC00958在宫颈癌组织中呈高表达(P<0.001),高水平的LINC00958与大肿瘤、晚期肿瘤分级、浸润深度和淋巴转移有关联(P<0.05或P<0.01)。与正常人宫颈上皮细胞ende1617相比,宫颈癌细胞中LINC00958水平均显著升高(P<0.01或P<0.001)。shLINC00958组SiHa细胞的增殖、迁移、侵袭能力及其培养上清液的促HLEC淋巴管形成能力均显著低于shNC组(P<0.05、P<0.01或P<0.001),LINC00958组Caski细胞的增殖、迁移、侵袭能力及其培养上清液的促HLEC淋巴管形成能力显著高于CMV组(P<0.05、P<0.01或P<0.001)。通过RNA下拉、RNA免疫沉淀实验发现宫颈癌细胞中LINC00958能够特异性结合VEGF-C。LINC00958+shVEGF-C组Caski细胞的增殖、迁移、侵袭能力及其培养上清液的促淋巴管形成能力显著低于LINC00958组(P<0.01或P<0.001);在小鼠腘淋巴结转移模型中,LINC00958+shVEGF-C组中小鼠腘窝淋巴结的体积和VEGF-C蛋白、N-cadherin蛋白以及LYVE-1的阳性细胞比例均显著低于LINC00958组(均P<0.001)。结论:LINC00958通过直接与VEGF-C蛋白相互作用增强宫颈癌细胞的增殖、侵袭、淋巴管生成能力,促进小鼠腘淋巴结转移模型的淋巴结转移。 展开更多
关键词 LINc00958 血管内皮生长因子c 宫颈癌 cASKI细胞 SIHA细胞 淋巴管形成 淋巴结转移
下载PDF
子宫内膜癌患者组织内PGK1、VEGF-C表达与病理特征和预后的关系
15
作者 王昆 陈强 《检验医学与临床》 CAS 2024年第9期1303-1306,共4页
目的探讨子宫内膜癌患者组织内磷酸甘油酸酯激酶1(PGK1)、血管内皮生长因子-C(VEGF-C)表达与病理特征和预后的关系。方法回顾性选取2017年5月至2020年5月榆林市第一医院收治的106例子宫内膜癌患者作为研究对象。采用免疫组化染色法检测... 目的探讨子宫内膜癌患者组织内磷酸甘油酸酯激酶1(PGK1)、血管内皮生长因子-C(VEGF-C)表达与病理特征和预后的关系。方法回顾性选取2017年5月至2020年5月榆林市第一医院收治的106例子宫内膜癌患者作为研究对象。采用免疫组化染色法检测子宫内膜癌患者癌组织与癌旁组织内PGK1、VEGF-C表达,比较子宫内膜癌患者癌组织与癌旁组织PGK1、VEGF-C表达。比较不同病理特征子宫内膜癌患者癌组织内PGK1、VEGF-C表达;采用Kaplan-Meier生存曲线分析不同PGK1、VEGF-C表达患者的预后情况。结果子宫内膜癌患者癌组织中PGK1高表达、VEGF-C阳性患者比例显著高于癌旁组织(P<0.05)。PGK1高表达患者低分化、FIGO分期Ⅲ~Ⅳ期和有淋巴结转移患者比例显著高于PGK1低表达患者(P<0.05),VEGF-C阳性患者FIGO分期Ⅲ~Ⅳ期和有淋巴结转移患者比例显著高于VEGF-C阴性患者(P<0.05)。106例子宫内膜癌患者3年生存率为79.25%(84/106);Kaplan-Meier生存曲线结果显示,子宫内膜癌组织PGK1高表达患者3年生存率[67.44%(29/43)]显著低于PGK1低表达患者[87.30%(55/63)],差异有统计学意义(Log-Rankχ^(2)=7.513,P<0.01);VEGF-C阳性患者3年生存率[75.00%(60/80)]显著低于VEGF-C阴性患者[92.31%(24/26)],差异有统计学意义(Lo-Rankχ^(2)=6.527,P<0.01)。结论PGK1、VEGF-C水平与子宫内膜癌的发生和发展密切相关,PGK1高表达、VEGF-C阳性患者疾病进展更严重,预后更差。 展开更多
关键词 子宫内膜癌 磷酸甘油酸酯激酶1 血管内皮生长因子-c 病理特征 预后
下载PDF
乳腺癌患者VEGF-C对血清TNF-α影响术后并发上肢淋巴水肿的中介效应
16
作者 邱军彦 李文涛 韩智培 《淮海医药》 CAS 2024年第3期245-249,共5页
目的:分析乳腺癌患者血管内皮生长因子-C(VEGF-C)在血清肿瘤坏死因子-α(TNF-α)与术后并发上肢淋巴水肿的中介效应。方法:选取2018年1月—2022年2月某院接受治疗的乳腺癌术后1年内并发上肢淋巴水肿患者90例为研究组,按照1∶1对照原则,... 目的:分析乳腺癌患者血管内皮生长因子-C(VEGF-C)在血清肿瘤坏死因子-α(TNF-α)与术后并发上肢淋巴水肿的中介效应。方法:选取2018年1月—2022年2月某院接受治疗的乳腺癌术后1年内并发上肢淋巴水肿患者90例为研究组,按照1∶1对照原则,选取该院同期术后1年内未发生上肢淋巴水肿的乳腺癌患者90例为对照组。随访1年,观察患者上肢淋巴水肿变化。比较2组患者及术后2年不同水肿分级的研究组间癌组织VEGF-C表达、血清TNF-α水平。采用Pearson相关系数分析术后1、2年,研究组患者癌组织VEGF-C表达与血清TNF-α、上肢淋巴水肿分级的相关性。构建AMOS 24.0结构方程模型,采用Bootstrap中介效应法评估术后1、2年,癌组织VEGF-C表达对血清TNF-α与术后并发上肢淋巴水肿关联的中介效应。结果:研究组VEGF-C表达阳性率低于对照组,TNF-α水平高于对照组,且不同水肿分级的研究组患者间TNF-α水平呈现:轻度组<中度组<重度组,差异均有统计学意义(P<0.05)。术后2年,研究组中有水肿患者VEGF-C表达阳性率低于无水肿患者,TNF-α水平高于无水肿患者,且不同水肿分级的研究组患者间TNF-α水平呈现:轻度组<中度组<重度组,差异均有统计学意义(P<0.05)。相关分析结果显示,术后1年,血清TNF-α水平与上肢淋巴水肿分级呈正相关(r=0.642,P<0.05);术后2年,癌组织VEGF-C表达与血清TNF-α、上肢淋巴水肿分级呈负相关(r=-0.685、-0.754,P<0.05),血清TNF-α与上肢淋巴水肿分级呈正相关(r=0.669,P<0.05)。中介效应分析显示,术后1年,血清TNF-α水平对术后并发上肢淋巴水肿具有直接预测作用(P<0.05);术后2年,VEGF-C在血清TNF-α影响术后并发上肢淋巴水肿时发挥中介效应。结论:血清TNF-α可通过VEGF-C间接诱导乳腺癌患者术后并发上肢淋巴水肿,尤其在术后2年仍出现水肿时,临床可在乳腺癌术后定期检测患者血清TNF-α,预防上肢淋巴水肿的发生。 展开更多
关键词 乳腺肿瘤 血管内皮生长因子-c 肿瘤坏死因子-α 上肢淋巴水肿 中介效应
下载PDF
Gecko crude peptides inhibit migration and lymphangiogenesis by down regulating the expression of VEGF-C in human hepatocellular carcinoma cells and human lymphatic endothelial cells 被引量:3
17
作者 Meng-li GUO Cai-e WANG +1 位作者 Yi-meng DUAN Jian-gang WANG 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2017年第10期958-959,共2页
OBJECTIVE To explore the role of gecko crude peptides(GCPs)in the proliferation,apoptosis,migration and lymphangiogenesis of human hepatocellular carcinoma cells(Hep G2)and human lymphaticendothelial cells(HLECs)in vi... OBJECTIVE To explore the role of gecko crude peptides(GCPs)in the proliferation,apoptosis,migration and lymphangiogenesis of human hepatocellular carcinoma cells(Hep G2)and human lymphaticendothelial cells(HLECs)in vitro.METHODS The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay was used to evaluate the anti-proliferative effect of GCPs and si RNA-VEGF-C on Hep G2 cells,Hoechst 33258 staining and flow cytometry were performed to analyze cycle and apoptosis.The migration and invasion ability of cells were assayed by transwell chamber experiment and wound-healing assay.The protein and m RNA expressions of vascular endothelial growth factor-C(VEGF-C)and CXC chemokine receptor-4(CXCR4)were detected by q-PCR,immunofluorescence,Western blot.The protein expressions of the extracellular signal regulated kinase(ERKI/2),c-Jun N-terminal kinase(JNK),p38-mitogen activated protein kinases(p38 MAPK),serine/threonine kinase(Akt)and phosphatidylinositol-3-kinase(PI3K)were detected by western blot.The anti-lymphangiogenesis effect of GCPs on the HLECs was analyzed using an in vitro tube-formation assay.The protein and m RNA expressions of vascular endothelial growth factor receptor-3(VEGFR-3)and stromal cell-derived factor-1(SDF-1)were detected by q-PCR,Western blot.RESULTS GCPs and si RNA-VEGF-C inhibited Hep G2 proliferation,invasion and migration,and the most obvious inhibitory effect was both synergistic effects.Thus,GCPs suppressed HLECs proliferation,migration and tubelike structure formationin a dose-dependent manner,and had inhibitory effect of tumor-induced lymphangiogenesis in vitro.Additionally,we found that GCPs and si RNA-VEGF-C decreased the expressions of MMP-2,MMP-9,VEGF-C,CXCR4,phospho-ERK1/2,phospho-P38,phospho-JNK and PI3K in Hep G2 cells.Moreover,GCPs had a dose-dependent depressive effecton the expressions of VEGFR-3,SDF-1 in HLECs.CONCLUSION The low expression of VEGF-C mediated by si RNA-VEGF-C and GCPs inhibit tumor proliferation,invasion and migrationby suppressing the MAPK signaling pathway through reduced levels of VEGF-C,and GCPs inhibit tumor lymphangiogenesis by suppressing the CXCR4/SDF-1 signaling pathway through suppressed VEGF-C/VEGFR-3. 展开更多
关键词 gecko crude peptides hepatic carcinoma vascular endothelial growth factor-c RNA interference(RNAi) LYMPHANGIOGENESIS
下载PDF
VEGF-C在胃腺癌组织中表达的意义
18
作者 张良良 单国平 +2 位作者 陈超 卞晓星 徐逸昕 《南通大学学报(医学版)》 2023年第1期12-15,共4页
目的:探讨胃腺癌组织中血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)蛋白的表达及其临床病理意义。方法:用免疫组织化学法检测胃腺癌及癌旁组织中VEGF-C蛋白的表达,分析其与肿瘤大小、浸润深度和淋巴结转移的关系... 目的:探讨胃腺癌组织中血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)蛋白的表达及其临床病理意义。方法:用免疫组织化学法检测胃腺癌及癌旁组织中VEGF-C蛋白的表达,分析其与肿瘤大小、浸润深度和淋巴结转移的关系。结果:VEGF-C蛋白在癌组织和癌旁组织中的阳性表达率分别为85.92%和36.62%(P<0.05);在直径<5 cm和≥5 cm肿瘤组织中的阳性表达率分别为73.91%和92.00%(P<0.05);在T_(1)/T_(2)和T_(3)/T_(4)患者中的阳性表达率分别为70.37%和95.45%(P<0.05);在N_(0)、N_(1)、N_(2)、N_(3)中的阳性表达率分别为52.94%、90.00%、100.00%、100.00%,差异有统计学意义(P<0.05);而其与年龄、性别无相关性(P>0.05);VEGF-C蛋白的表达影响患者的生存期。结论:VEGF-C蛋白在胃腺癌组织中高表达,并与肿瘤的生长、浸润及淋巴结转移有相关性。 展开更多
关键词 胃癌 血管内皮生长因子c 淋巴结转移
下载PDF
降钙素、血管内皮生长因子C在甲状腺髓样癌患者中的表达及临床意义
19
作者 张昆 黄华 +2 位作者 武晓娜 邢珂 郑朝阳 《癌症进展》 2024年第8期914-916,928,共4页
目的探讨降钙素(Ctn)、血管内皮生长因子C(VEGFC)在甲状腺髓样癌(MTC)患者中的表达及临床意义。方法选取82例MTC患者和71例健康体检者,分别作为观察组和对照组,采用酶联免疫吸附试验检测Ctn和VEGFC水平。比较两组受试者及不同TNM分期MT... 目的探讨降钙素(Ctn)、血管内皮生长因子C(VEGFC)在甲状腺髓样癌(MTC)患者中的表达及临床意义。方法选取82例MTC患者和71例健康体检者,分别作为观察组和对照组,采用酶联免疫吸附试验检测Ctn和VEGFC水平。比较两组受试者及不同TNM分期MTC患者的Ctn、VEGFC水平。对MTC患者随访1年,根据随访情况分为预后良好组和预后不良组。比较预后良好组和预后不良组MTC患者的临床特征,采用Logistic回归模型分析MTC患者预后的影响因素。结果观察组患者Ctn、VEGFC水平均明显高于对照组,差异均有统计学意义(P﹤0.01)。Ⅲ~Ⅳ期MTC患者Ctn、VEGFC水平均明显高于Ⅰ~Ⅱ期患者,差异均有统计学意义(P﹤0.01)。预后良好组和预后不良组患者的TNM分期、淋巴结转移情况、Ctn水平、VEGFC水平比较,差异均有统计学意义(P﹤0.05)。多因素Logistic回归分析结果显示,TNM分期为Ⅲ~Ⅳ期、淋巴结转移、Ctn水平升高、VEGFC水平升高均是MTC患者预后不良的独立危险因素(P﹤0.05)。结论Ctn、VEGFC在MTC患者中表达均升高,且其表达水平与MTC的发生发展及预后密切相关。 展开更多
关键词 甲状腺髓样癌 降钙素 血管内皮生长因子c 预后
下载PDF
SULT2A1、VEGF-C、IGF-Ⅰ在子宫肌瘤患者中的表达及相关性研究
20
作者 严小芳 夏天 周平 《中国性科学》 2023年第12期77-81,共5页
目的探讨硫酸基转移酶2A1(SULT2A1)、血管内皮生长因子-C(VEGF-C)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在子宫肌瘤患者中的表达及相关性。方法选取2020年12月至2022年12月江苏大学附属宜兴市人民医院病理科保存的50例女性子宫肌瘤组织(子宫肌... 目的探讨硫酸基转移酶2A1(SULT2A1)、血管内皮生长因子-C(VEGF-C)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在子宫肌瘤患者中的表达及相关性。方法选取2020年12月至2022年12月江苏大学附属宜兴市人民医院病理科保存的50例女性子宫肌瘤组织(子宫肌瘤组织组)及瘤旁组织(瘤旁组织组)标本,并选取同时期同一医院病理科保存的65例正常子宫肌组织(正常子宫肌组织组)作为研究对象。采用免疫组化法检测各标本SULT2A1、VEGF-C蛋白,采用流式细胞术检测IGF-Ⅰ的表达;绘制受试者工作特征(ROC)曲线分析SULT2A1、VEGF-C、IGF-Ⅰ单独检测及联合检测对子宫肌瘤的诊断价值;分析SULT2A1、VEGF-C、IGF-Ⅰ表达之间的相关性。结果相比于正常子宫肌组织及瘤旁组织,子宫肌瘤组织VEGF-C、IGF-Ⅰ表达较高,SULT2A1表达较低(P<0.05)。瘤旁组织VEGF-C、IGF-Ⅰ表达高于正常子宫肌组织,SULT2A1低于正常子宫肌组织(P<0.05)。相关性分析显示,SULT2A1和VEGF-C表达呈负相关(r=-0.902,P=0.001),IGF-Ⅰ和VEGF-C表达呈正相关(r=0.860,P=0.001),SULT2A1和IGF-Ⅰ表达呈负相关(r=-0.854,P=0.001);绘制ROC曲线显示,VEGF-C、IGF-Ⅰ、SULT2A1单独预测价值低于三项联合。结论VEGF-C、IGF-Ⅰ在子宫肌瘤组织中高表达,SULT2A1在子宫肌瘤组织中低表达,三者具有相关性,VEGF-C、IGF-Ⅰ高表达及SULT2A1低表达是子宫肌瘤患者发病的风险预测因子。 展开更多
关键词 硫酸基转移酶2A1 血管内皮生长因子-c 胰岛素样生长因子-Ⅰ 子宫肌瘤
下载PDF
上一页 1 2 58 下一页 到第
使用帮助 返回顶部