Aim: To determine whether vasectomy away from the epididymal tail (via the inguinal canal) in rabbits can reduce the early postoperative effects on spermatogenesis. Methods: Twenty-nine normal male Japanese white ...Aim: To determine whether vasectomy away from the epididymal tail (via the inguinal canal) in rabbits can reduce the early postoperative effects on spermatogenesis. Methods: Twenty-nine normal male Japanese white rabbits (aged 4- 6 months) were subjected to unilateral close-ended (conventional) or open-ended (the cut end of the juxta-epididymal vas deferens not ligated) vasectomy via the inguinal canal. Ten days and 3 months after operation, testes, epididymides and vasa deferentia were removed and methacrylate resin-embedded sections prepared. The histology of the testis, epididymis and vas deferens was examined under light microscope, and the volume and diameter of the seminiferous tubules were quantitatively studied using stereological methods. Results: Neither of the methods of vasectomy led to apparent damage to spermatogenesis on the vasectomized side in comparison with the contralateral shamoperated side, but the juxta-epididymal vas deferens on the vasectomized side was highly distended and contained numerous sperm 3 months after operation. Conclusion: Vasectomy away from the cauda epididymis has no significant early postoperative effects on spermatogenesis in rabbits.展开更多
To study the functional changes of immunological survcillsnce after vasectomy,we hzve dynamically obscrved the influence of vasectonly on the functions of T lymphocytes and macrophages of peripheral blood from vasecto...To study the functional changes of immunological survcillsnce after vasectomy,we hzve dynamically obscrved the influence of vasectonly on the functions of T lymphocytes and macrophages of peripheral blood from vasectomized animals as the exPerimental model. The results showed that: (1) PHA and Con-A induced mitogenesis of T lymphocytes from the rabbits vasectomized for eight months was significantly inhibited (P<0.01) and recovered in rabbits vasectoomized for 12, 18 and 22 months respectively, (2) The IL -2 secretion from peripheral blood mononuclcar cells (PBMCs) in the rabbits vasectomized for 8 months was reduced remarkably (P<0.01) and reached the normal level in the rabbits vasectomlied for 12 months; (3) A significant correlation was found betwecn Con-A induced mitogenesis of T lymphocytes and the IL-2 secrcted from PBMCs (r=0.661, P<0.01); (4) The IL- 1 secrction form periPhoal blood macrophsges induced by LPS significantly increased in the rabbits vasectonlized for S months (P<0.02) and returned to the normal level in the rabbits vasectomized for 12, 18 and 22 months respectively, (5) The levl of plasma lysozyme increased significantly in the rabbits vasectomized for 8 months (P<0.05) and returned to the normal in the rabbits vasectomized for 12 months, (6) The level of IL- 1 secreted by the macrophages and the level of plasma lysozyme were closely. Correlated (r=0.576, P<0.001). The present results may provide an expeimantal evidence for the evaluating the functional changes of immunological surveillance in vasectomized animals.展开更多
Objective - The effects of vasectomy on testes and related structures are disputable. The aim of this study was to determine whether the reproductive organs of rabbits were influenced after 12 months vasectomy. Study ...Objective - The effects of vasectomy on testes and related structures are disputable. The aim of this study was to determine whether the reproductive organs of rabbits were influenced after 12 months vasectomy. Study design – Bilateral vasectomy was performed on 10 male adult New Zealand Big Eared White Rabbits (another 10 rabbits set as sham operated group) and tissue samples were examined by light microscopy after 12 months treatment. The histologic alterations were observed and analyzed by stereology method. Cell apoptosis by Terminal Deoxynucleotidyl Transferase Mediated dUTP Nick End Labeling (TUNEL) assay were employed. Result - Bilateral vasectomy for 12 months affected the morphology of testis, epididymis and vas deferens tissues. Significant changes were noted in the histological stereology analysis. The apoptosis of the cells on the testis, epididymis and vas deferens increased compared with the sham operated group. Conclusion - Our findings suggest that the reproductive tissues of rabbits were influenced in a relatively longer term vasectomy.展开更多
文摘Aim: To determine whether vasectomy away from the epididymal tail (via the inguinal canal) in rabbits can reduce the early postoperative effects on spermatogenesis. Methods: Twenty-nine normal male Japanese white rabbits (aged 4- 6 months) were subjected to unilateral close-ended (conventional) or open-ended (the cut end of the juxta-epididymal vas deferens not ligated) vasectomy via the inguinal canal. Ten days and 3 months after operation, testes, epididymides and vasa deferentia were removed and methacrylate resin-embedded sections prepared. The histology of the testis, epididymis and vas deferens was examined under light microscope, and the volume and diameter of the seminiferous tubules were quantitatively studied using stereological methods. Results: Neither of the methods of vasectomy led to apparent damage to spermatogenesis on the vasectomized side in comparison with the contralateral shamoperated side, but the juxta-epididymal vas deferens on the vasectomized side was highly distended and contained numerous sperm 3 months after operation. Conclusion: Vasectomy away from the cauda epididymis has no significant early postoperative effects on spermatogenesis in rabbits.
文摘To study the functional changes of immunological survcillsnce after vasectomy,we hzve dynamically obscrved the influence of vasectonly on the functions of T lymphocytes and macrophages of peripheral blood from vasectomized animals as the exPerimental model. The results showed that: (1) PHA and Con-A induced mitogenesis of T lymphocytes from the rabbits vasectomized for eight months was significantly inhibited (P<0.01) and recovered in rabbits vasectoomized for 12, 18 and 22 months respectively, (2) The IL -2 secretion from peripheral blood mononuclcar cells (PBMCs) in the rabbits vasectomized for 8 months was reduced remarkably (P<0.01) and reached the normal level in the rabbits vasectomlied for 12 months; (3) A significant correlation was found betwecn Con-A induced mitogenesis of T lymphocytes and the IL-2 secrcted from PBMCs (r=0.661, P<0.01); (4) The IL- 1 secrction form periPhoal blood macrophsges induced by LPS significantly increased in the rabbits vasectonlized for S months (P<0.02) and returned to the normal level in the rabbits vasectomized for 12, 18 and 22 months respectively, (5) The levl of plasma lysozyme increased significantly in the rabbits vasectomized for 8 months (P<0.05) and returned to the normal in the rabbits vasectomized for 12 months, (6) The level of IL- 1 secreted by the macrophages and the level of plasma lysozyme were closely. Correlated (r=0.576, P<0.001). The present results may provide an expeimantal evidence for the evaluating the functional changes of immunological surveillance in vasectomized animals.
文摘Objective - The effects of vasectomy on testes and related structures are disputable. The aim of this study was to determine whether the reproductive organs of rabbits were influenced after 12 months vasectomy. Study design – Bilateral vasectomy was performed on 10 male adult New Zealand Big Eared White Rabbits (another 10 rabbits set as sham operated group) and tissue samples were examined by light microscopy after 12 months treatment. The histologic alterations were observed and analyzed by stereology method. Cell apoptosis by Terminal Deoxynucleotidyl Transferase Mediated dUTP Nick End Labeling (TUNEL) assay were employed. Result - Bilateral vasectomy for 12 months affected the morphology of testis, epididymis and vas deferens tissues. Significant changes were noted in the histological stereology analysis. The apoptosis of the cells on the testis, epididymis and vas deferens increased compared with the sham operated group. Conclusion - Our findings suggest that the reproductive tissues of rabbits were influenced in a relatively longer term vasectomy.