The interaction of L-Aspartic acid （Asp） with Vctoria Green S （VGS） in pH 6.0 Britton-Robinson buffer solution has been investigated by UV/Vis spectropbotometry and resonance light scattering （RLS） technique. Th...The interaction of L-Aspartic acid （Asp） with Vctoria Green S （VGS） in pH 6.0 Britton-Robinson buffer solution has been investigated by UV/Vis spectropbotometry and resonance light scattering （RLS） technique. The non-covalent interactions such as hydrogen bond, salt linkage and hydrophobic bond were proposed to explain the interaction between VGS and Asp. It indicated that Asp could link each other to form a long chain by salt linkages and hydrogen bonds in aqueous medium. Then the long chain surrounded the hydrophobic groups of VGS via hydrophobic interaction and reacted with VGS by salt linkages and hydrogen bonds to form a macromolecular aggregation. The aggregation was much bigger than VGS itself. So the color became deeper and the system＇s absorption and RLS intensity increased. The increase in the absorbance at 617nm was proportional to the concentration of Asp, providing a basis for the quantitative determination of Asp. This method was simple and efficient than the ordinary methods and had been applied to the direct determination of Asp with satisfactory results.展开更多
基金Acknowledgements： This work was supported by the Natural Science Foundation of Anhui Province （No. 2006K J128B） and the Young Teacher Natural Science Foundation of Anhui Province Commission （No. 2006jp 1139）.
文摘The interaction of L-Aspartic acid （Asp） with Vctoria Green S （VGS） in pH 6.0 Britton-Robinson buffer solution has been investigated by UV/Vis spectropbotometry and resonance light scattering （RLS） technique. The non-covalent interactions such as hydrogen bond, salt linkage and hydrophobic bond were proposed to explain the interaction between VGS and Asp. It indicated that Asp could link each other to form a long chain by salt linkages and hydrogen bonds in aqueous medium. Then the long chain surrounded the hydrophobic groups of VGS via hydrophobic interaction and reacted with VGS by salt linkages and hydrogen bonds to form a macromolecular aggregation. The aggregation was much bigger than VGS itself. So the color became deeper and the system＇s absorption and RLS intensity increased. The increase in the absorbance at 617nm was proportional to the concentration of Asp, providing a basis for the quantitative determination of Asp. This method was simple and efficient than the ordinary methods and had been applied to the direct determination of Asp with satisfactory results.
