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Support vector regression-based operational effectiveness evaluation approach to reconnaissance satellite system 被引量:1
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作者 HAN Chi XIONG Wei +1 位作者 XIONG Minghui LIU Zhen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第6期1626-1644,共19页
As one of the most important part of weapon system of systems(WSoS),quantitative evaluation of reconnaissance satellite system(RSS)is indispensable during its construction and application.Aiming at the problem of nonl... As one of the most important part of weapon system of systems(WSoS),quantitative evaluation of reconnaissance satellite system(RSS)is indispensable during its construction and application.Aiming at the problem of nonlinear effectiveness evaluation under small sample conditions,we propose an evaluation method based on support vector regression(SVR)to effectively address the defects of traditional methods.Considering the performance of SVR is influenced by the penalty factor,kernel type,and other parameters deeply,the improved grey wolf optimizer(IGWO)is employed for parameter optimization.In the proposed IGWO algorithm,the opposition-based learning strategy is adopted to increase the probability of avoiding the local optima,the mutation operator is used to escape from premature convergence and differential convergence factors are applied to increase the rate of convergence.Numerical experiments of 14 test functions validate the applicability of IGWO algorithm dealing with global optimization.The index system and evaluation method are constructed based on the characteristics of RSS.To validate the proposed IGWO-SVR evaluation method,eight benchmark data sets and combat simulation are employed to estimate the evaluation accuracy,convergence performance and computational complexity.According to the experimental results,the proposed method outperforms several prediction based evaluation methods,verifies the superiority and effectiveness in RSS operational effectiveness evaluation. 展开更多
关键词 reconnaissance satellite system(Rss) support vector regression(SVR) gray wolf optimizer opposition-based learning parameter optimization effectiveness evaluation
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Application of Auto-regressive Linear Model in Understanding the Effect of Climate on Malaria Vectors Dynamics in the Three Gorges Reservoir
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作者 WANG Duo Quan GU Zheng Cheng +2 位作者 ZHENG Xiang GUO Yun TANG Lin Hua 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2014年第10期811-814,共4页
It is important to understand the dynamics of malaria vectors in implementing malaria control strategies. Six villages were selected from different sections in the Three Gorges Reservoir fc,r exploring the relationshi... It is important to understand the dynamics of malaria vectors in implementing malaria control strategies. Six villages were selected from different sections in the Three Gorges Reservoir fc,r exploring the relationship between the climatic |:actors and its malaria vector density from 1997 to 2007 using the auto-regressive linear model regressi^n method. The result indicated that both temperature and precipitation were better modeled as quadratic rather than linearly related to the density of Anopheles sinensis. 展开更多
关键词 Application of Auto-regressive Linear Model in Understanding the effect of Climate on Malaria vectors Dynamics in the Three Gorges Reservoir AUTO
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After effect6.5中Vector paint原理及其特殊应用
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作者 徐丽珍 徐永青 郑蓉 《现代电视技术》 2005年第11期116-117,共2页
After effect是一个适用于高端视频系统的专业型非线性编 辑创作系统。它提供了基于帧的视频设计途径。也为影视制作 人员提供了强大的影片编辑和制作工具。本文主要介绍After effect6.5中的新增功能——Vector paint的原理及其特殊的... After effect是一个适用于高端视频系统的专业型非线性编 辑创作系统。它提供了基于帧的视频设计途径。也为影视制作 人员提供了强大的影片编辑和制作工具。本文主要介绍After effect6.5中的新增功能——Vector paint的原理及其特殊的应用。 展开更多
关键词 AFTER effect vector PAINT MASK Keyframe 应用 原理 视频系统 非线性编辑
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Pressure and velocity cross-spectrum of normal modes in low-frequency acoustic vector field of shallow water and its application 被引量:2
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作者 Yun Yu Qing Ling Jiang Xu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2015年第2期241-249,共9页
The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especi... The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especially the active component of the pressure and horizontal particle velocity cross-spectrum, also called ho- rizontal complex cross acoustic intensity, when only two normal modes are trapped in the waveguide. Both the approximate theo- retic analysis and the numerical results show that the sign of the horizontal complex cross acoustic intensity active component is independent of the range when vertically deployed receiving dual sensors are placed in appropriate depths, the sum of which is equal to the waveguide effective depth, so it can be used to tell whether the sound source is near the surface or underwater; while the range rate is expected to be measured by utilizing the sign distribution characteristic of the reactive component. The further robustness analysis of the depth classification algorithm shows that the existence of shear waves in semi infinite basement and the change of acoustic velocity profiles have few effects on the application of this method, and the seabed attenuation will limit the detection range, but the algorithm still has a good robustness in the valid detection range. 展开更多
关键词 low frequency acoustic vector field effective depth complex cross acoustic intensity active component target depth classificatio
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Flatness intelligent control via improved least squares support vector regression algorithm 被引量:2
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作者 张秀玲 张少宇 +1 位作者 赵文保 徐腾 《Journal of Central South University》 SCIE EI CAS 2013年第3期688-695,共8页
To overcome the disadvantage that the standard least squares support vector regression(LS-SVR) algorithm is not suitable to multiple-input multiple-output(MIMO) system modelling directly,an improved LS-SVR algorithm w... To overcome the disadvantage that the standard least squares support vector regression(LS-SVR) algorithm is not suitable to multiple-input multiple-output(MIMO) system modelling directly,an improved LS-SVR algorithm which was defined as multi-output least squares support vector regression(MLSSVR) was put forward by adding samples' absolute errors in objective function and applied to flatness intelligent control.To solve the poor-precision problem of the control scheme based on effective matrix in flatness control,the predictive control was introduced into the control system and the effective matrix-predictive flatness control method was proposed by combining the merits of the two methods.Simulation experiment was conducted on 900HC reversible cold roll.The performance of effective matrix method and the effective matrix-predictive control method were compared,and the results demonstrate the validity of the effective matrix-predictive control method. 展开更多
关键词 least squares support vector regression multi-output least squares support vector regression FLATNEss effective matrix predictive control
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Mosquito vector management with botanicals-the most effective weapons in controlling mosquito-borne diseases 被引量:2
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作者 Shyamapada Mandal 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第4期336-336,共1页
Dear editor,Mosquito-borne diseases,viz.,malaria,filariasis,dengue hemorrhagic fever,chikungunya,Japanese encephalitis etc.,created huge impact on humans over the world,and the chemical insecticides remain the mainsta... Dear editor,Mosquito-borne diseases,viz.,malaria,filariasis,dengue hemorrhagic fever,chikungunya,Japanese encephalitis etc.,created huge impact on humans over the world,and the chemical insecticides remain the mainstay of effective control.But,indiscriminate and rampant use of the chemical insecticides in controlling mosquito vectors,in order to prevent diseases vectored by them,has resulted problems related to the adverse environmental effects for their(insecticides)potential toxicity,high operational cost,community acceptance,and the development of insecticide resistance among the vectors.The factors mentioned above prompted the search for new means of control strategies.Currently,the botanicals(viz.,plant extracts,essential oils and phytochemicals)with mosquitocidal potential 展开更多
关键词 ppm LC Mosquito vector management with botanicals-the most effective weapons in controlling mosquito-borne diseases
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Spin Supercurrent in Phenomena of Quantum Non-Locality (Quantum Correlations, Magnetic Vector Potential) and in Near-Field Antenna Effect 被引量:1
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作者 Liudmila B. Boldyreva 《Journal of Modern Physics》 2019年第2期128-144,共17页
It is shown that such phenomena as quantum correlations (interaction of space-separated quantum entities), the action of magnetic vector potential on quantum entities in the absence of magnetic field, and near-field a... It is shown that such phenomena as quantum correlations (interaction of space-separated quantum entities), the action of magnetic vector potential on quantum entities in the absence of magnetic field, and near-field antenna effect (the existence of superluminally propagating electromagnetic fields) may be explained by action of spin supercurrents. In case of quantum correlations between quantum entities, spin supercurrent emerges between virtual particles pairs (virtual photons) created by those quantum entities. The explanation of magnetic vector potential and near-field antenna effect is based on contemporary principle of quantum mechanics: the physical vacuum is not an empty space but the ground state of the field consisting of quantum harmonic oscillators (QHOs) characterized by zero-point energy. Using the properties of the oscillators and spin supercurrent, it is proved that magnetic vector potential is proportional to the moment causing the orientation of spin of QHO along the direction of magnetic field. The near-field antenna effect is supposed to take place as a result of action of spin supercurrent causing secondary electromagnetic oscillations. In this way, the electromagnetic field may spread at the speed of spin supercurrent. As spin supercurrent is an inertia free process, its speed may be greater than that of light, which does not contradict postulates of special relativity that sets limits to the speed of inertial systems only. 展开更多
关键词 SPIN SUPERCURRENT QUANTUM Correlations Magnetic vector Potential NEAR-FIELD Antenna effect Zero-Point Energy QUANTUM Harmonic Oscillator VIRTUAL Particles Pair VIRTUAL Photon
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Huberized Multiclass Support Vector Machine for Microarray Classification 被引量:7
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作者 LI Jun-Tao JIA Ying-Min 《自动化学报》 EI CSCD 北大核心 2010年第3期399-405,共7页
关键词 基因 支持向量机 计算方法 路径系数
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EFFECT OF ELECTROACUPUNCTURE ON CANINE PYLORIC PRESSURE AND ITS RELATION TO SOMATOSTATIN AND NITRIC OXIDE
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作者 黄裕新 孙大勇 +2 位作者 褚自宏 高巍 王庆莉 《World Journal of Acupuncture-Moxibustion》 1999年第4期43-46,共4页
Purpose of the present study: To explore the effect of electroacupuncture (EA) on canine pyloric pressure and its relation to somatostain (SS) of blood plasma and nitric oxide (NO).Methods: An intragastric pressure de... Purpose of the present study: To explore the effect of electroacupuncture (EA) on canine pyloric pressure and its relation to somatostain (SS) of blood plasma and nitric oxide (NO).Methods: An intragastric pressure detector and radioimmunoassay (RIA) and biochemical assay are used to determine changes of pyloric pressure and frequency, contents of SS and NO of blood plasma after EA stimulation of different acupoints. Results: Following EA of Zusanli (ST 36), the total pressure, basic pressure and systolic frequency of the sphincter muscle of pylorus lowered, plasma SS content decreased and NO content increased. After EA of Shangjuxu (ST 37), the total pressure, basic pressure and systolic frequency of the sphincter muscle of pylorus decreased, plasrna NO content increased but changes of these indexes are less obvious than those of EA of Zusanli (ST 36). These indexes in other groups had no any apparent changes. Conclusion: EA could lower pyloric pressure and systolic frequency of the pyloric sphincter muscle, whicb may be the important pathway of EA in regulating functional activities of the stomach and intestines. The effect of EA is associated with the contents of some plasma biological substances affecting pyloric pressure and the action of meridians and acupoints is of specificity to a certain degree. 展开更多
关键词 EA Gastric PREssURE ss NO effect of EA
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Two properties of sequence of vector measures on effect algebras
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作者 LIN Qing-shui LI Rong-lu 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2010年第4期475-480,共6页
Let (L, ,0, 1) be an effect algebra and let X be a Banach space. A function : L→ X is called a vector measure if μ(a b) =μ(a) + μ(b) whenever a⊥b in L. The function μ is said to be 8-bounded if limn... Let (L, ,0, 1) be an effect algebra and let X be a Banach space. A function : L→ X is called a vector measure if μ(a b) =μ(a) + μ(b) whenever a⊥b in L. The function μ is said to be 8-bounded if limn→∞μ(an) = 0 in X for any orthogonal sequence (an)n∈N in L. In this paper, we introduce two properties of sequence of s-bounded vector measures and give some results on these properties. 展开更多
关键词 effect algebra s-bounded vector measure.
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Dynamics of Quantum State and Effective Hamiltonian with Vector Differential Form of Motion Method
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作者 Long Xiong Wei-Feng Zhuang Ming Gong 《Chinese Physics Letters》 SCIE EI CAS CSCD 2022年第7期36-40,共5页
Effective Hamiltonians in periodically driven systems have received widespread attention for realization of novel quantum phases, non-equilibrium phase transition, and Majorana mode. Recently, the study of effective H... Effective Hamiltonians in periodically driven systems have received widespread attention for realization of novel quantum phases, non-equilibrium phase transition, and Majorana mode. Recently, the study of effective Hamiltonian using various methods has gained great interest. We consider a vector differential equation of motion to derive the effective Hamiltonian for any periodically driven two-level system, and the dynamics of the spin vector are an evolution under the Bloch sphere. Here, we investigate the properties of this equation and show that a sudden change of the effective Hamiltonian is expected. Furthermore, we present several exact relations, whose expressions are independent of the different starting points. Moreover, we deduce the effective Hamiltonian from the high-frequency limit, which approximately equals the results in previous studies. Our results show that the vector differential equation of motion is not affected by a convergence problem, and thus, can be used to numerically investigate the effective models in any periodic modulating system. Finally, we anticipate that the proposed method can be applied to experimental platforms that require time-periodic modulation, such as ultracold atoms and optical lattices. 展开更多
关键词 Dynamics of Quantum State and effective Hamiltonian with vector Differential Form of Motion Method HAMILTONIAN vector QUANTUM
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Recombinant adenoviral vector expressing the tumor necrosis factor-related apoptosis-inducing ligand gene suppresses human pancreatic cancer growth
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作者 Rui Tian Renyi Qin Zhiyong Du Wei Xia Chengjian Shi 《The Chinese-German Journal of Clinical Oncology》 CAS 2007年第5期464-468,共5页
Objective: To investigate the antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene transfection mediated by adenovirus into human pancreatic carcinoma cell line Panc-1, and the mech... Objective: To investigate the antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene transfection mediated by adenovirus into human pancreatic carcinoma cell line Panc-1, and the mechanisms involved in this effect. Methods: TRAIL gene was transfected into pancreatic cancer cell line Panc-1 by an adenovirus vector (Ad-TRAIL). Level of TRAIL mRNA expression was determined using RT-PCR, and TRAIL protein synthesis was evaluated with Western blot. Cell-growth activities were determined by MTT assay. The bystander effect was observed by co-culturing the Panc-1 cells with the transfected TRAIL gene at different ratios. Apoptosis in pancreatic cancer cells was detected by flow cytometry. Procaspase-8 and procaspase-3 were determined by Western blot. Results: The stable overexpression of TRAIL was de-tected in Panc-1 cells transfected by Ad-TRAIL. Ad-TRAIL significantly inhibited of cell viability of Panc-1 cells. Furthermore, co-culture of cancer cells transfected with TRAIL with that nontransfected resulted in the cell death of both cells by bystander effect. Moreover, the percentage of apoptotic cells was significantly higher in the Ad-TRAIL-treatment group compared to the control groups (P < 0.01). And there was a diminished amount of procaspase-8 and procaspase-3 after infection with Ad-TRAIL. Conclusion: The overexpression of TRAIL gene in Panc-1 cells by Ad-TRAIL exerts its antitumor effects, and the mechanisms involved in this effect may be proapoptosis and bystander effect. 展开更多
关键词 pancreatic carcinoma adenovirus vector TRAIL APOPTOSIS bystander effect
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On the Massless Vector Fields in a Rindler Space
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作者 Roberto Soldati Caterina Specchia 《Journal of Modern Physics》 2015年第12期1743-1755,共13页
We study the quantum theory of the mass-less vector fields on the Rindler space. We evaluate the Bogoliubov coefficients by means of a new technique based upon the use of light-front coordinates and Mellin transform. ... We study the quantum theory of the mass-less vector fields on the Rindler space. We evaluate the Bogoliubov coefficients by means of a new technique based upon the use of light-front coordinates and Mellin transform. We briefly comment about the ensuing Unruh effect and its consequences. 展开更多
关键词 Rindler SPACE Unruh effect vector FIELDS BOGOLIUBOV COEFFICIENTS
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Effect of Dislocations on Helium Bubble Nucleation in 316L SS During Hot-implantation at 550℃
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作者 Zhang Chonghong Chen Keqin Wang Yinshu and Sun Jiguang(General Research Institute of Nonferrous Metals,Beijing.) 《IMP & HIRFL Annual Report》 1994年第0期173-174,共2页
Effect of Dislocations on Helium Bubble Nucleation in 316L SS During Hot-implantation at 550℃¥ZhangChonghong... Effect of Dislocations on Helium Bubble Nucleation in 316L SS During Hot-implantation at 550℃¥ZhangChonghong;ChenKeqin;WangYi... 展开更多
关键词 BUBBLE IMPLANTATION NUCLEATION effect HELIUM HOT Dislocations ss During in
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Forward modelling of the Cotton-Mouton effect polarimetry on EAST tokamak
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作者 沈敏勇 张际波 +4 位作者 张耀 揭银先 刘海庆 谢锦林 丁卫星 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第3期100-117,共18页
Measurement of plasma electron density by far-infrared laser polarimetry has become a routine and indispensable tool in magnetic confinement fusion research.This article presents the design of a Cotton-Mouton polarime... Measurement of plasma electron density by far-infrared laser polarimetry has become a routine and indispensable tool in magnetic confinement fusion research.This article presents the design of a Cotton-Mouton polarimeter interferometer,which provides a reliable density measurement without fringe jumps.Cotton-Mouton effect on Experimental Advanced Superconducting Tokamak(EAST)is studied by Stokes equation with three parameters(s_(1),s_(2),s_(3)).It demonstrates that under the condition of a small Cotton-Mouton effect,parameter s_(2)contains information about Cotton-Mouton effect which is proportional to the line-integrated density.For a typical EAST plasma,the magnitude of Cotton-Mouton effects is less than 2πfor laser wavelength of 432μm.Refractive effect due to density gradient is calculated to be negligible.Time modulation of Stokes parameters(s_(2),s_(3))provides heterodyne measurement.Due to the instabilities arising from laser oscillation and beam refraction in plasmas,it is necessary for the system to be insensitive to variations in the amplitude of the detection signal.Furthermore,it is shown that non-equal amplitude of X-mode and O-mode within a certain range only affects the DC offset of Stokes parameters(s_(2),s_(3))but does not greatly influence the phase measurements of Cotton-Mouton effects. 展开更多
关键词 EAST Cotton-Mouton effect polarimeter interferometer electron density measurement Stokes vector
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The Aharonov-Bohm Effect: An Exploration of Quantum Interference and Electromagnetic Potentials
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作者 Bahman Zohuri 《Journal of Energy and Power Engineering》 CAS 2024年第3期79-87,共9页
The AB(Aharonov-Bohm)effect is a pivotal quantum mechanical phenomenon that illustrates the fundamental role of the electromagnetic vector potential A in determining the phase of a charged particle’s wave function,ev... The AB(Aharonov-Bohm)effect is a pivotal quantum mechanical phenomenon that illustrates the fundamental role of the electromagnetic vector potential A in determining the phase of a charged particle’s wave function,even in regions where the magnetic field B is zero.This effect demonstrates that quantum particles are influenced not only by the fields directly present but also by the potentials associated with those fields.In the AB effect,an electron beam is split into two paths,with one path encircling a solenoid and the other bypassing it.Despite the absence of a magnetic field in the regions traversed by the beams,the vector potential A associated with the magnetic flux Φ through the solenoid induces a phase shift in the electron’s wave function.This phase shift,quantified by △φ=qΦ/hc,manifests as a change in the interference pattern observed in the detection screen.The phenomenon underscores the principle of gauge invariance in QED(quantum electrodynamics),where physical observables remain invariant under local gauge transformations of the vector and scalar potentials.This reinforces the notion that the vector potential A has a profound impact on quantum systems,beyond its classical role.This article outlines the AB effect,including its theoretical framework,experimental observations,and implications.The focus on the role of the vector potential in quantum mechanics provides a comprehensive understanding of this important phenomenon. 展开更多
关键词 AB effect vector potential(A) magnetic flux(Φ) quantum phase shift gauge invariance electromagnetic potentials interference pattern scalar fields longitudinal waves QED
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Construction of Cox7a2 fluorescent vector and its effect on cytochrome C oxidase activity in mouse Sertoli cell line TM4
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作者 刘保兴 《外科研究与新技术》 2011年第4期260-260,共1页
Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell l... Objective To construct Cox7a2 fluorescent vector and study its effect on cytochrome C oxidase ( COX) activity in mouse Sertoli cell line TM4. Methods The coding region of CoxTa2 was amplified from mouse Sertoli cell line TM4 by RT-PCR. PCR product was 展开更多
关键词 line cell Construction of Cox7a2 fluorescent vector and its effect on cytochrome C oxidase activity in mouse Sertoli cell line TM4 TM
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Bio-Electromagnetics without Fields: The Effect of the Vector Potential
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作者 Andras Szasz 《Open Journal of Biophysics》 2021年第2期205-224,共20页
Numerous considerations deal with specialties of bioelectromagnetic effects, including the force-free and field-free interactions. The fact that bioelectromagnetic phenomena consist of effects without mechanical force... Numerous considerations deal with specialties of bioelectromagnetic effects, including the force-free and field-free interactions. The fact that bioelectromagnetic phenomena consist of effects without mechanical forces and even without measurable fields looks impossible in the simple considerations. However, the stochastic fluctuations cause surprising results, with scientifically proven bioelectromagnetism in field-free conditions. In the first steps, we show the scalar and vector potentials’ specialties instead of electric and magnetic fields defined by the well-known Maxwellian equations. The vanishing of the fields is connected to the potentials’ stochastic fluctuations, the noises control the “zero-ground”. The result shows a possibility of a wave that has no attenuation during its transmission through the material. In this meaning, the result is similar to the consequences of the scalar-wave (SW) considerations. The structural changes follow a particular noise spectrum (called pink-noise or 1/f noise), which keeps the entropy constant in a broad range of scaling magnification. 展开更多
关键词 vector-Potential Scalar-Potential Stochastic-Processes Field-Free effects Curl-Free Fields AXIAL-vector Life HOMEOSTASIS Entropy 1/f Noise
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基于PNN/PCA/SS-SVR的光伏发电功率短期预测方法 被引量:49
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作者 王昕 黄柯 +3 位作者 郑益慧 李立学 郎永波 吴昊 《电力系统自动化》 EI CSCD 北大核心 2016年第17期156-162,共7页
光伏发电功率预测对太阳能开发利用、电网稳定安全运行具有重要意义。提出一种融合了概率神经网络(PNN)、主成分分析法(PCA)、分散搜索(SS)和支持向量机回归(SVR)的光伏输出功率预测模型。首先结合天气信息通过PNN将天气划分为晴、多云... 光伏发电功率预测对太阳能开发利用、电网稳定安全运行具有重要意义。提出一种融合了概率神经网络(PNN)、主成分分析法(PCA)、分散搜索(SS)和支持向量机回归(SVR)的光伏输出功率预测模型。首先结合天气信息通过PNN将天气划分为晴、多云、阴、雨4种类型,然后在每种天气类型下,利用PCA对影响光伏出力的多个气象因素,如太阳辐射强度、温度和相对湿度等进行降维、转换成少数几个主成分作为输入向量,最后建立SS算法优化SVR的光伏发电功率短期预测模型。结果表明,该模型实现了对不同天气类型下的光伏出力较为精准的预测,具有一定的可行性及指导意义。 展开更多
关键词 光伏发电功率预测 概率神经网络 主成分分析法 分散搜索 支持向量机回归
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小麦SBEⅡa、SSⅡa基因片段的克隆及VIGS重组载体的构建 被引量:2
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作者 李淼淼 南富波 +1 位作者 刘伟 李卫华 《新疆农业科学》 CAS CSCD 北大核心 2013年第11期1961-1966,共6页
【目的】克隆小麦SBEⅡa、SSⅡa基因部分序列并构建二者的VIGS重组载体,为在小麦上利用VIGS技术研究SBEⅡa、SSⅡa基因与小麦淀粉合成的关系奠定基础。【方法】根据GenBank中公布的小麦SBEⅡa(AF286319.1)、SSⅡa(AF155217.2)基因序列... 【目的】克隆小麦SBEⅡa、SSⅡa基因部分序列并构建二者的VIGS重组载体,为在小麦上利用VIGS技术研究SBEⅡa、SSⅡa基因与小麦淀粉合成的关系奠定基础。【方法】根据GenBank中公布的小麦SBEⅡa(AF286319.1)、SSⅡa(AF155217.2)基因序列分别设计特异引物,利用RT-PCR技术克隆SBEⅡa、SSⅡa基因的特异片段。然后以BSMV:γ-PDS为原载体,通过酶切连接用SBEⅡa、SSⅡa基因片段分别将原载体中的PDS基因进行替换,从而构建BSMV:γ-SBEⅡa和BSMV:γ-SSⅡa重组载体。【结果】克隆的SBEⅡa和SSⅡa基因片段长分别是178 bp和171 bp。序列分析表明:SBEⅡa基因片段与小麦SBEⅡa(AF286319.1)序列同源性为100%;、SSⅡa基因片段与SSⅡa(AF155217.2)序列同源性也为100%;酶切鉴定结果表明成功构建BSMV:γ-SBEⅡa和BSMV:γ-SSⅡa重组载体。【结论】构建的BSMV:γ-SBEⅡa和BSMV:γ-SSⅡa重组载体将为下一步利用VIGS技术在小麦上研究SBEⅡa、SSⅡa基因与小麦淀粉合成的关系奠定基础。 展开更多
关键词 小麦 SBEⅡa ssⅡa VIGS载体
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