Improvement of Phenols Production by Amino Acids in Callus Cultures of Verbascum thapsus L.

A great mullein (Verbascum thapsus L.) was a famous species in Scrophulariaceae family. It was generally used as herbal medicine. Explants of V. thapsus (leaves and petioles) were cultured in vitro on Murashige and Skoog (MS) medium for shoot proliferation.Plantlet explants were cultured on MS medium supplemented with combination of Benzyl adenine (BA) and Naphthalene acetic acid (NAA) for callus induction. The best fresh and dry weight of callus formation was achieved using 0.5 mg/l BA. Quantitative analyses with High-performance liquid chromatography (HPLC) showed the content of phenols like Coumarin, Eugenol and Thymol were relatively low in leaves of mother plant, (10, 41, 310 ppm) respectively. The addition of different concentrations of amino acids as a precursor adding separately to the tissue culture medium led to raise the accumulation levels of phenolic compounds in callus tissue. Generally, the enhancement of accumulation depended on the type of amino acids and their concentration. The results showed 150 mg/l of Proline encouraged production of Comarin to 2752%, while 50 mg/l of Proline promoted accumulation of Eugenol to 290%. Whilst 150 mg/l of Tryptophan increased production of Thymol to 390%, in comparison with mother plant.

基于保留指数的GC-MS分析毛蕊花挥发性成分

采用水蒸气蒸馏法采集毛蕊花中的挥发性成分,气相色谱/质谱联用技术(GC-MS)结合保留指数对其进行分析鉴定,共鉴定出38种主要的挥发性成分,含量较高的组分分别为柠檬烯26.57%、茴香酮24.81%、桉叶素7.24%、石竹烯氧化物5.91%和蒎烯4.72%。GC-MS结合保留指数对毛蕊花挥发性成分进行定性分析,使定性结果更为准确。

毛蕊花的生药学研究

目的:对毛蕊花进行生药学鉴定,为其鉴别及应用提供科学依据。方法:采用来源鉴定、性状鉴定、显微及理化鉴定的方法。结果:详细描述了毛蕊花的生药学鉴定特征。结论:实验结果可为该生药的鉴定、质量标准的制定和进一步开发利用提供理论依据。

GC-MS法比较分析毛蕊花和果实中挥发油化学成分

目的分析鉴定毛蕊花VerbascumthapsusL.鲜花、干花、鲜果实及干果实中的挥发油的组成及相对含量。方法水蒸气蒸馏提取,GC-MS法进行分析鉴定,面积归一化法测定各组分含量。结果分别从毛蕊鲜花、干花、鲜果实及干果实的挥发油提取物中确证了50、48、28和26个化合物。结论表明毛蕊花中的化学成分十分丰富,GC-MS可以对毛蕊花挥发油进行良好的分离鉴定。