Introduction:Verruca vulgaris is one of the most common low-risk HPV infections and is characterized by excessive proliferation of keratinocytes.Currently,very little genetic information is available regarding verruca...Introduction:Verruca vulgaris is one of the most common low-risk HPV infections and is characterized by excessive proliferation of keratinocytes.Currently,very little genetic information is available regarding verruca vulgaris in the Chinese population.This study aimed to obtain comprehensive transcript information of verruca vulgaris by RNA sequencing.Methods:High-throughput sequencing was performed on three fresh verruca vulgaris samples and adjacent normal skin on the Illumina sequencing platform.The transcriptomes were analyzed using bioinformatics and the differentially expressed genes(DEGs)were verified by immunohistochemistry.Verruca vulgaris exhibited a unique molecular signature.Results:In total,1,643 DEGs were identified in verruca vulgaris compared to normal skin.The functions of the DEGs were studies by Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,DEGs Reactome analysis,disease annotation function,and STRING protein-protein interaction(PPI)network analysis.The results revealed 595 GO terms associated with the cell cycle,signal transduction,immune system,signaling molecules,and interaction.The Reactome analysis revealed enrichment in reversible hydration of carbon dioxide and BMP signaling,while the disease annotation function revealed that the enriched DEGs are involved in keratosis disorders.The STRING PPI network showed that the edges with the highest density mainly included the 2′-5′oligoadenylate synthase(OAS)family-related proteins.Furthermore,the M-code analysis found ISG15,IRF7,and OASL were scored as significant modules and their high expression compared to the control was verified by immunohistochemistry.Conclusion:These findings contribute to the genetic information of verruca vulgaris in the Chinese population,revealing that interferon-stimulated genes may play essential roles in verruca vulgaris.展开更多
BACKGROUND Epidermolytic acanthoma(EA)is a rare benign skin lesion,usually found in the genital area of men and women,with epidermolytic hyperkeratosis as its distinguishing histologic characteristic.It is commonly mi...BACKGROUND Epidermolytic acanthoma(EA)is a rare benign skin lesion,usually found in the genital area of men and women,with epidermolytic hyperkeratosis as its distinguishing histologic characteristic.It is commonly misdiagnosed as condyloma accuminatum,verruca,and seborrheic keratosis.Since this lesion is benign,treatment is not necessary.However,it is often misdiagnosed,and patients are likely to undergo incorrect counseling and unnecessary treatment,causing undue burden to the patient.This study seeks to increase awareness of this rare condition to prevent future misdiagnoses.CASE SUMMARY A 55-year-old man living with human immunodeficiency virus presented for anal cancer screening.His physical examination revealed a flesh colored papule at the anal margin.The initial differential diagnosis included molluscum contagiosum,anal condyloma,and basal cell carcinoma.The lesion was excised to obtain a definitive diagnosis and was discovered to be EA.CONCLUSION EA is often misdiagnosed due to its similarity to other dermatologic conditions.Careful examination and pathologic evaluation should be obtained to ensure proper diagnosis.展开更多
A female student,23 years old,suffering from multiple warts scattered on the right sole for 2 years and pain on the bottom of the first metatarsal bone when walking for 1 month.The case was diagnosed as kujinjian(枯筋...A female student,23 years old,suffering from multiple warts scattered on the right sole for 2 years and pain on the bottom of the first metatarsal bone when walking for 1 month.The case was diagnosed as kujinjian(枯筋箭)in traditional Chinese medicine and verruca plantaris in western medicine.At the beginning of treatment,fire needling therapy was applied to local lesions,once every two days.Afterwards,moxibustion with moxa cone was used at the local lesions,once a day,three cones on each lesion till warts were dropped.Simultaneously,in combination,mild moxibustion was given at bilateral Zusanli(足三里ST36)for 15 min,once a day.After three times of moxibustion therapy,a part of warts were crusted and 3 weeks later,all the warts were dropped.No recurrence was found in the follow-up visit.The therapeutic methods of this case are simple in manipulation and satisfactory in therapeutic effect.展开更多
基金The National Natural Science Foundation of China(Grant No.81903227)supported our study.
文摘Introduction:Verruca vulgaris is one of the most common low-risk HPV infections and is characterized by excessive proliferation of keratinocytes.Currently,very little genetic information is available regarding verruca vulgaris in the Chinese population.This study aimed to obtain comprehensive transcript information of verruca vulgaris by RNA sequencing.Methods:High-throughput sequencing was performed on three fresh verruca vulgaris samples and adjacent normal skin on the Illumina sequencing platform.The transcriptomes were analyzed using bioinformatics and the differentially expressed genes(DEGs)were verified by immunohistochemistry.Verruca vulgaris exhibited a unique molecular signature.Results:In total,1,643 DEGs were identified in verruca vulgaris compared to normal skin.The functions of the DEGs were studies by Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,DEGs Reactome analysis,disease annotation function,and STRING protein-protein interaction(PPI)network analysis.The results revealed 595 GO terms associated with the cell cycle,signal transduction,immune system,signaling molecules,and interaction.The Reactome analysis revealed enrichment in reversible hydration of carbon dioxide and BMP signaling,while the disease annotation function revealed that the enriched DEGs are involved in keratosis disorders.The STRING PPI network showed that the edges with the highest density mainly included the 2′-5′oligoadenylate synthase(OAS)family-related proteins.Furthermore,the M-code analysis found ISG15,IRF7,and OASL were scored as significant modules and their high expression compared to the control was verified by immunohistochemistry.Conclusion:These findings contribute to the genetic information of verruca vulgaris in the Chinese population,revealing that interferon-stimulated genes may play essential roles in verruca vulgaris.
文摘BACKGROUND Epidermolytic acanthoma(EA)is a rare benign skin lesion,usually found in the genital area of men and women,with epidermolytic hyperkeratosis as its distinguishing histologic characteristic.It is commonly misdiagnosed as condyloma accuminatum,verruca,and seborrheic keratosis.Since this lesion is benign,treatment is not necessary.However,it is often misdiagnosed,and patients are likely to undergo incorrect counseling and unnecessary treatment,causing undue burden to the patient.This study seeks to increase awareness of this rare condition to prevent future misdiagnoses.CASE SUMMARY A 55-year-old man living with human immunodeficiency virus presented for anal cancer screening.His physical examination revealed a flesh colored papule at the anal margin.The initial differential diagnosis included molluscum contagiosum,anal condyloma,and basal cell carcinoma.The lesion was excised to obtain a definitive diagnosis and was discovered to be EA.CONCLUSION EA is often misdiagnosed due to its similarity to other dermatologic conditions.Careful examination and pathologic evaluation should be obtained to ensure proper diagnosis.
基金Supported by Henan Province Higher Education Teaching Reform Research and Practice Project:2019SJGLX077Y。
文摘A female student,23 years old,suffering from multiple warts scattered on the right sole for 2 years and pain on the bottom of the first metatarsal bone when walking for 1 month.The case was diagnosed as kujinjian(枯筋箭)in traditional Chinese medicine and verruca plantaris in western medicine.At the beginning of treatment,fire needling therapy was applied to local lesions,once every two days.Afterwards,moxibustion with moxa cone was used at the local lesions,once a day,three cones on each lesion till warts were dropped.Simultaneously,in combination,mild moxibustion was given at bilateral Zusanli(足三里ST36)for 15 min,once a day.After three times of moxibustion therapy,a part of warts were crusted and 3 weeks later,all the warts were dropped.No recurrence was found in the follow-up visit.The therapeutic methods of this case are simple in manipulation and satisfactory in therapeutic effect.
