Novel Vesicular Aqueous Two-Phase Systems

The interesting phenopmena of two aqueous phases coexisting in dilute aqueous solutions of sodium undecenoate-dodecyltrimethylammo brodride ndxture and sodium laurate-dodecyltrimethylammonium brondde mixture were investigated. Vesicles existing in both phases were shown by TEM images. The vesicles are dispersed in lower phase and flocculated in upper phase. Multilamellar structure of vesicles was found in the upper phase of sodium laurate-dodecyltrimethylammonium bromide system.

胆汁泡相与微胶粒相的蛋白质表达谱对比观察

目的采用比较蛋白质组学技术研究胆固醇结石患者胆囊胆汁的胆汁泡相与微胶粒相的蛋白质表达谱的差异，为探索胆石成因提供实验依据。方法密度梯度超速离心法制备胆汁泡相和微胶粒相，提取蛋白，构建相应的蛋白质表达谱，Image Master图像分析软件获得差异蛋白点信息，质谱鉴定差异表达蛋白。结果胆汁泡相与微胶粒相总蛋白浓度分别为（1．5358±0．0682）mg／ml、（7．1222±0．2022）mg／ml（P〈0．01）。两组组内蛋白质平均匹配点数分别为120±24和198±37，组间72±16个蛋白质点相互匹配，匹配率为45．30％。胆汁泡相与微胶粒相成功鉴定出差异蛋白质点8个，泡相中6个点表达上调，2个点表达下调。视黄醇结合蛋白（RBP）和白蛋白（HSA）丰度差异为免疫印迹法证实。结论初步建立了胆同醇结石患者胆囊胆汁的胆汁泡相和微胶粒相蛋白表达谱，分析、鉴定了差异蛋白点，为筛选胆固醇结石形成关键调控子提供依据。