以新鲜葫芦蜂为原料,利用抗坏血酸结合超高压技术抑制多酚氧化酶的活性,通过SDS和Native电泳筛选出多酚氧化酶条带,并进一步利用质谱技术鉴定其性质。结果表明,抗坏血酸与超高压结合对多酚氧化酶的抑制有协同效果,用质量分数为0.16%的...以新鲜葫芦蜂为原料,利用抗坏血酸结合超高压技术抑制多酚氧化酶的活性,通过SDS和Native电泳筛选出多酚氧化酶条带,并进一步利用质谱技术鉴定其性质。结果表明,抗坏血酸与超高压结合对多酚氧化酶的抑制有协同效果,用质量分数为0.16%的抗坏血酸结合300 MPa超高压处理的效果比单独使用抗坏血酸好;电泳显示多酚氧化酶表观分子量约为120 k Da,质谱给出了多酚氧化酶的名称、覆盖率和蛋白序列等性质。展开更多
The Yellow-legged hornet, Vespa velutina , was accidentally introduced in France in 2004, and then spread rapidly through the French territory but also to adjacent European countries (Spain, Portugal, and Belgium). Du...The Yellow-legged hornet, Vespa velutina , was accidentally introduced in France in 2004, and then spread rapidly through the French territory but also to adjacent European countries (Spain, Portugal, and Belgium). During summer and autumn, V. velutina workers hunt domestic honeybees, Apis mellifera, for feeding their larvae. The impact of this alien species is mainly economic, beekeepers experiencing heavy colony losses, but also ecological, V. velutina hunting other pollinators. In their year-round life cycle, nest initiation by single queen during spring is the critical stage. In invaded areas, spring queen trapping using food baits has been promoted by apicultural unions in order to limit V. velutina population expansion. The goals of this work were 1) to evaluate the yield of this method, 2) to identify appropriate sites for trapping, 3) to identify potential optimal climatic windows of capture, and 4) to quantify the impact on local entomofauna. Our results showed that water proximity enhances trapping but not beehives proximity, and that trapping is inefficient if average week temperatures are below 10°C. Although the trapping effect on biodiversity should be studied more carefully, spring queen trapping is highly questionable unless specific attractants could be proposed.展开更多
文摘以新鲜葫芦蜂为原料,利用抗坏血酸结合超高压技术抑制多酚氧化酶的活性,通过SDS和Native电泳筛选出多酚氧化酶条带,并进一步利用质谱技术鉴定其性质。结果表明,抗坏血酸与超高压结合对多酚氧化酶的抑制有协同效果,用质量分数为0.16%的抗坏血酸结合300 MPa超高压处理的效果比单独使用抗坏血酸好;电泳显示多酚氧化酶表观分子量约为120 k Da,质谱给出了多酚氧化酶的名称、覆盖率和蛋白序列等性质。
文摘The Yellow-legged hornet, Vespa velutina , was accidentally introduced in France in 2004, and then spread rapidly through the French territory but also to adjacent European countries (Spain, Portugal, and Belgium). During summer and autumn, V. velutina workers hunt domestic honeybees, Apis mellifera, for feeding their larvae. The impact of this alien species is mainly economic, beekeepers experiencing heavy colony losses, but also ecological, V. velutina hunting other pollinators. In their year-round life cycle, nest initiation by single queen during spring is the critical stage. In invaded areas, spring queen trapping using food baits has been promoted by apicultural unions in order to limit V. velutina population expansion. The goals of this work were 1) to evaluate the yield of this method, 2) to identify appropriate sites for trapping, 3) to identify potential optimal climatic windows of capture, and 4) to quantify the impact on local entomofauna. Our results showed that water proximity enhances trapping but not beehives proximity, and that trapping is inefficient if average week temperatures are below 10°C. Although the trapping effect on biodiversity should be studied more carefully, spring queen trapping is highly questionable unless specific attractants could be proposed.
