患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢...患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。展开更多
Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in med...Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in mediating antibiotic resistance.Methods:Antibiotic susceptibility and minimum inhibitory concentration(MIC) values of antibiotics for the isolated V.cholerae 01 Ogawa(n=12) were determined by disk diffusion and agar dilution methods,respectively,using ampicillin(Am),chloramphenicol(C),trimethoprim(Tm),tetracycline(T).erythromycine(Er), nalidixic acid(Nx).ciprofloxacin(Cp),amikacin(Ak) and cefotaxime(Cf).Plasmid curing of multidrug resistant(MDR) V.cholerae 01 Ogawa strains was done following ethidium bromide treatment.Following electrophoresis,the plasmid DNAs,extracted from the isolated MDR V. cholerae O1 Ogawa strains and their cured derivatives,were visualized and documented in ’gel doc’ system.Results:The outbreak causing V.cholerae O1 Ogawa isolates were MDR as determined by disk diffusion susceptibility test,and MIC determination.The isolates showed three different drug resistance patterns:AmTmTErNx(for 6 isolates).TmTErCp(for 5 isolates), and AmTniNx(for one isolate),and showed uniform sensitivity to C,Ak and Cf.The loss of plasmids with the concomitant loss of resistance to Am,Tm,T and Er of the isolates occurred following ethidium bromide treatment.Conclusions:The current findings suggest that the V. cholerae O1 Ogawa associated with the cholera outbreak were MDR,and resistance to Am,Tm,T and Er among the isolates were plasmid mediated.展开更多
文摘患者,男性,58岁,乙型肝炎后肝硬化失代偿期、2型糖尿病12年,近1个月再发腹胀、水肿,尿少入院。住院期间患者寒战、发热,取血标本进行培养,选用头孢哌酮/舒巴坦经验性抗感染治疗。确诊非O1/O139霍乱弧菌引起的血流感染,药敏试验提示头孢哌酮/舒巴坦敏感后,继续使用当前药物,头孢哌酮/舒巴坦由3 g q12h改为3 g q8h治疗。10 d后患者无发热,血培养和粪便霍乱弧菌培养阴性,好转出院。
文摘Objective:To determine the antibiotic resistance of Vibrio choleras(V.cholerae) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in mediating antibiotic resistance.Methods:Antibiotic susceptibility and minimum inhibitory concentration(MIC) values of antibiotics for the isolated V.cholerae 01 Ogawa(n=12) were determined by disk diffusion and agar dilution methods,respectively,using ampicillin(Am),chloramphenicol(C),trimethoprim(Tm),tetracycline(T).erythromycine(Er), nalidixic acid(Nx).ciprofloxacin(Cp),amikacin(Ak) and cefotaxime(Cf).Plasmid curing of multidrug resistant(MDR) V.cholerae 01 Ogawa strains was done following ethidium bromide treatment.Following electrophoresis,the plasmid DNAs,extracted from the isolated MDR V. cholerae O1 Ogawa strains and their cured derivatives,were visualized and documented in ’gel doc’ system.Results:The outbreak causing V.cholerae O1 Ogawa isolates were MDR as determined by disk diffusion susceptibility test,and MIC determination.The isolates showed three different drug resistance patterns:AmTmTErNx(for 6 isolates).TmTErCp(for 5 isolates), and AmTniNx(for one isolate),and showed uniform sensitivity to C,Ak and Cf.The loss of plasmids with the concomitant loss of resistance to Am,Tm,T and Er of the isolates occurred following ethidium bromide treatment.Conclusions:The current findings suggest that the V. cholerae O1 Ogawa associated with the cholera outbreak were MDR,and resistance to Am,Tm,T and Er among the isolates were plasmid mediated.
