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霍乱弧菌O_(139)菌毛的提取及鉴定
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作者 杜艳 贾文祥 +6 位作者 刘聪 刘莉 吴晓容 张再容 尹致英 孙丽 孙大锡 《华西医科大学学报》 CAS CSCD 1999年第1期28-30,共3页
为给霍乱弧菌亚单位菌苗的研制提供基础,作者对霍乱弧菌O139菌毛的提取和鉴定方法进行了探讨。结果发现,菌毛的最佳表达条件为AKI或CFA培养基中30℃静止培养24~36小时。SDS-PAGE电泳证实了TcpA蛋白的存... 为给霍乱弧菌亚单位菌苗的研制提供基础,作者对霍乱弧菌O139菌毛的提取和鉴定方法进行了探讨。结果发现,菌毛的最佳表达条件为AKI或CFA培养基中30℃静止培养24~36小时。SDS-PAGE电泳证实了TcpA蛋白的存在,分子量为20.5kd。用鼠抗毒素共调菌毛(TCP)的单克隆抗体和羊抗鼠IgG-HRP作斑点酶免疫试验,结果为阳性。用TCP免疫大白兔所获得的免疫血清与O139型和ElTor型霍乱弧菌菌株作常规凝集试验,可见TCP与E1Tor型菌株发生交叉反应。由此表明TCP可作为一种有效的保护抗原。 展开更多
关键词 霍乱弧菌 菌毛 毒素共调 菌毛 霍乱菌苗
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Lessons from Vibrio Pathogen and the Comparative Study of Vaccines Developed
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作者 Tania Rahman Md Ferdous Seraj Silvia Buroni 《Advances in Microbiology》 2018年第12期950-964,共15页
Cholera continues to be one of the most common causes of morbidity and mortality among children and adults in developing countries. Vaccine against cholera is an approach in the control of this epidemic and pandemic d... Cholera continues to be one of the most common causes of morbidity and mortality among children and adults in developing countries. Vaccine against cholera is an approach in the control of this epidemic and pandemic disease. From the development of very early oral cholera vaccine, advances in vaccine development documented due to a good illustration of the epidemiology, outbreak strategy, and pathophysiology of the disease causing pathogen. The newer-generation oral cholera vaccines are safe and guarantee a high level of protection during outbreak settings for several years. Yet infants and young children in developing countries are hyporesponsive to vaccines and show poor protection against cholera. In this review, we survey and analyse our current knowledge on the etiology of cholera, its clinical manifestation, global epidemiology and elaborate the vaccine candidates, which are effective against the pathogen and the corresponding immune responses to the available vaccines. These reviews comprehensively cover the salient features of recent discoveries related to Vibrio cholerae virulence, past and present vaccine candidates and their advantages and disadvantages with their development strategies. We believe that the advances that have been included in this review will give a comprehensive insight to the prevention and control of cholera outbreaks and development of effective cholera vaccines. 展开更多
关键词 vibrio cholerae SEROGROUP O1 O139 cholera Toxin LPS vibriocidal ANTIBODIES IgA ANTIBODIES Heat-Killed Whole Cell VACCINE Live Attenuated Oral VACCINE
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