Vicia villosa is an annual legume plant,and is mainly used for green manure by farmers in southwest China.Field growth experiments were performed on six plots.The concentrations of mineral nutrients and soluble sugar,...Vicia villosa is an annual legume plant,and is mainly used for green manure by farmers in southwest China.Field growth experiments were performed on six plots.The concentrations of mineral nutrients and soluble sugar,and the changes of carbon and nitrogen isotopic composition within and among organs of Vicia were deter- mined.Significant differences in legume growth were found in response to soil type and its moisture conditions.The Vicia villosa was relatively well adapted to growth in limestone soils than sandstone soils.The distribution of sugar concentrations andδ1 3C-differences between roots and leaves indicate that the translocation of sugars from leaves to roots may be restricted by soil drought.Therefore,there was an inhibition of Pi distribution from roots to leaves, resulting in over optimum threshold of N/P ratio.Those may originate from the feedback regulation in the legume, where soluble sugar could not be distributed from leaves to roots.The results ofδ1 5N values in tissues suggest that there should be different preferential use of nitrogen resource by legume during the formation of nodules:before nodule formation the legume preferentially utilizes inorganic nitrogen from soils,but afterwards the nitrogen should be mainly from N2-fixation.Our results indicate that the lack of nodulation development,except for S2,should be ascribed to the factor controlling bi-direction nutrient transfer,which should be efficiency of establishment symbiosis with arbuscular mycorrhiza before nodulation formation.It is predicted that the species of Vicia villosa should be a legume associated with dual symbiosis with rhizobia and mycorrhiza.展开更多
[Objective] The paper was to explore the effects of ferrum and molybdenum nutrition on root nodule and physiological growth of Vicia villosa in seedling stage. [Method] A local cultivar of Qujing city,Vicia villosa,wa...[Objective] The paper was to explore the effects of ferrum and molybdenum nutrition on root nodule and physiological growth of Vicia villosa in seedling stage. [Method] A local cultivar of Qujing city,Vicia villosa,was studied using soilless cultivation method,and the effects of ferrum,molybdenum nutrition on root nodule and physiological growth of V. villosa in seedling stage were explored by adding 0. 01 mmol/L sodium molybdate and 0. 2 mmol/L ferrous sulfate respectively or simultaneously in the Hoagland's nutrient solution with the removal of ferrum and molybdenum salt. [Result]Ferrum and molybdenum significantly increased the number and weight of root nodule; the effect of single molybdenum treatment was the best,and the number and weight of root nodule in single molybdenum treatment were nearly 100% and 150% higher than that of the control. After treated by ferrum and molybdenum nutrition,the stem length and biomass of V. villosa decreased at varying levels,while root length and root shoot ratio increased significantly; the decrease extent of stem length in ferrum treatment and composite treatment was greater than that in molybdenum treatment,and the increase extent of root length in molybdenum treatment was the greatest. Application of ferrum and molybdenum fertilizer significantly improved ferrum and molybdenum content of V. villosa plants,and their soluble protein and free amino acid content increased at varying levels; single application of ferrum led to the greatest increase,while phosphorus content decreased at varying levels.The soluble sugar content of V. villosa decreased significantly at 90 d post ferrum and molybdenum application; application of molybdenum fertilizer reduced the starch content while ferrum fertilizer increased the starch content of V. villosa. Moreover,application of ferrum and molybdenum fertilizer reduced the SOD activity and enhanced the POD activity of V. villosa. [Conclusion] The application of ferrum and molybdenum fertilizer promotes the growth of root nodule and V. villosa plant.展开更多
基金financially supported by the National Natural Science Foundation of China (Grant No.40772207)the Ministry of Science and Technology of China(Grant No.2006CB403200)
文摘Vicia villosa is an annual legume plant,and is mainly used for green manure by farmers in southwest China.Field growth experiments were performed on six plots.The concentrations of mineral nutrients and soluble sugar,and the changes of carbon and nitrogen isotopic composition within and among organs of Vicia were deter- mined.Significant differences in legume growth were found in response to soil type and its moisture conditions.The Vicia villosa was relatively well adapted to growth in limestone soils than sandstone soils.The distribution of sugar concentrations andδ1 3C-differences between roots and leaves indicate that the translocation of sugars from leaves to roots may be restricted by soil drought.Therefore,there was an inhibition of Pi distribution from roots to leaves, resulting in over optimum threshold of N/P ratio.Those may originate from the feedback regulation in the legume, where soluble sugar could not be distributed from leaves to roots.The results ofδ1 5N values in tissues suggest that there should be different preferential use of nitrogen resource by legume during the formation of nodules:before nodule formation the legume preferentially utilizes inorganic nitrogen from soils,but afterwards the nitrogen should be mainly from N2-fixation.Our results indicate that the lack of nodulation development,except for S2,should be ascribed to the factor controlling bi-direction nutrient transfer,which should be efficiency of establishment symbiosis with arbuscular mycorrhiza before nodulation formation.It is predicted that the species of Vicia villosa should be a legume associated with dual symbiosis with rhizobia and mycorrhiza.
基金Supported by National Natural Science Foundation of China(31660559)Science Research Project of Kunming University(XJZZ1604)Construction of Key and Dominant Disciplines(Ecology)of Universities and Colleges in Yunnan Province
文摘[Objective] The paper was to explore the effects of ferrum and molybdenum nutrition on root nodule and physiological growth of Vicia villosa in seedling stage. [Method] A local cultivar of Qujing city,Vicia villosa,was studied using soilless cultivation method,and the effects of ferrum,molybdenum nutrition on root nodule and physiological growth of V. villosa in seedling stage were explored by adding 0. 01 mmol/L sodium molybdate and 0. 2 mmol/L ferrous sulfate respectively or simultaneously in the Hoagland's nutrient solution with the removal of ferrum and molybdenum salt. [Result]Ferrum and molybdenum significantly increased the number and weight of root nodule; the effect of single molybdenum treatment was the best,and the number and weight of root nodule in single molybdenum treatment were nearly 100% and 150% higher than that of the control. After treated by ferrum and molybdenum nutrition,the stem length and biomass of V. villosa decreased at varying levels,while root length and root shoot ratio increased significantly; the decrease extent of stem length in ferrum treatment and composite treatment was greater than that in molybdenum treatment,and the increase extent of root length in molybdenum treatment was the greatest. Application of ferrum and molybdenum fertilizer significantly improved ferrum and molybdenum content of V. villosa plants,and their soluble protein and free amino acid content increased at varying levels; single application of ferrum led to the greatest increase,while phosphorus content decreased at varying levels.The soluble sugar content of V. villosa decreased significantly at 90 d post ferrum and molybdenum application; application of molybdenum fertilizer reduced the starch content while ferrum fertilizer increased the starch content of V. villosa. Moreover,application of ferrum and molybdenum fertilizer reduced the SOD activity and enhanced the POD activity of V. villosa. [Conclusion] The application of ferrum and molybdenum fertilizer promotes the growth of root nodule and V. villosa plant.
