目的比较分析新型冠状病毒病例咽拭子与痰标本的病毒核酸检测效果。方法对4例新型冠状病毒确诊病例的咽拭子与痰标本分别进行人体细胞GAPDH管家基因、病毒ORF 1ab基因、N基因及S基因Real time RT-PCR核酸检测与比较。结果4例病例的咽拭...目的比较分析新型冠状病毒病例咽拭子与痰标本的病毒核酸检测效果。方法对4例新型冠状病毒确诊病例的咽拭子与痰标本分别进行人体细胞GAPDH管家基因、病毒ORF 1ab基因、N基因及S基因Real time RT-PCR核酸检测与比较。结果4例病例的咽拭子和痰标本中,人体细胞管家基因GAPDH均呈现明显典型的扩增信号曲线;病毒ORF 1ab基因、N基因及S基因核酸检测中,痰标本的扩增曲线信号均比咽拭子强,扩增曲线的CT值均低于咽拭子,在病例1和4表现更加明显,而病例4的咽拭子标本检测中,商品化试剂呈现阴性结果,而痰标本则呈现明显的阳性结果。结论在开展新型冠状病毒实验室核酸检测中,痰标本的病毒含量高于咽拭子标本,其检测效果优于咽拭子标本。展开更多
Hepatitis C virus(HCV) is a potent human pathogen and is one of the main causes of chronic hepatitis round the world. The present review describes the evidencebased consensus on the diagnosis, prevention and managemen...Hepatitis C virus(HCV) is a potent human pathogen and is one of the main causes of chronic hepatitis round the world. The present review describes the evidencebased consensus on the diagnosis, prevention and management of HCV disease. Various techniques, for the detection of anti-HCV immunoglobulin G immunoassays, detection of HCV RNA by identifying virus-specific molecules nucleic acid testings, recognition of core antigen for diagnosis of HCV, quantitative antigenassay, have been used to detect HCV RNA and core antigen. Advanced technologies such as nanoparticlebased diagnostic assays, loop-mediated isothermal amplification and aptamers and Ortho trak-C assay have also come to the front that provides best detection results with greater ease and specificity for detection of HCV. It is of immense importance to prevent this infection especially among the sexual partners, injecting drug users, mother-to-infant transmission of HCV, household contact, healthcare workers and people who get tattoos and piercing on their skin. Management of this infection is intended to eradicate it out of the body of patients. Management includes examining the treatment(efficacy and protection), assessment of hepatic condition before commencing therapy, controlling the parameters upon which dual and triple therapies work, monitoring the body after treatment and adjusting the co-factors. Examining the treatment in some special groups of people(HIV/HCV co-infected, hemodialysis patients, renal transplanted patients).展开更多
文摘目的比较分析新型冠状病毒病例咽拭子与痰标本的病毒核酸检测效果。方法对4例新型冠状病毒确诊病例的咽拭子与痰标本分别进行人体细胞GAPDH管家基因、病毒ORF 1ab基因、N基因及S基因Real time RT-PCR核酸检测与比较。结果4例病例的咽拭子和痰标本中,人体细胞管家基因GAPDH均呈现明显典型的扩增信号曲线;病毒ORF 1ab基因、N基因及S基因核酸检测中,痰标本的扩增曲线信号均比咽拭子强,扩增曲线的CT值均低于咽拭子,在病例1和4表现更加明显,而病例4的咽拭子标本检测中,商品化试剂呈现阴性结果,而痰标本则呈现明显的阳性结果。结论在开展新型冠状病毒实验室核酸检测中,痰标本的病毒含量高于咽拭子标本,其检测效果优于咽拭子标本。
文摘Hepatitis C virus(HCV) is a potent human pathogen and is one of the main causes of chronic hepatitis round the world. The present review describes the evidencebased consensus on the diagnosis, prevention and management of HCV disease. Various techniques, for the detection of anti-HCV immunoglobulin G immunoassays, detection of HCV RNA by identifying virus-specific molecules nucleic acid testings, recognition of core antigen for diagnosis of HCV, quantitative antigenassay, have been used to detect HCV RNA and core antigen. Advanced technologies such as nanoparticlebased diagnostic assays, loop-mediated isothermal amplification and aptamers and Ortho trak-C assay have also come to the front that provides best detection results with greater ease and specificity for detection of HCV. It is of immense importance to prevent this infection especially among the sexual partners, injecting drug users, mother-to-infant transmission of HCV, household contact, healthcare workers and people who get tattoos and piercing on their skin. Management of this infection is intended to eradicate it out of the body of patients. Management includes examining the treatment(efficacy and protection), assessment of hepatic condition before commencing therapy, controlling the parameters upon which dual and triple therapies work, monitoring the body after treatment and adjusting the co-factors. Examining the treatment in some special groups of people(HIV/HCV co-infected, hemodialysis patients, renal transplanted patients).
