Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, s...Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, such as aseptic meningitis, brain stem encephalitis, and even death. There are still no effective clinical drugs used for the prevention and treatment of HFMD. Studying EV71 protein function is essential for elucidating the EV71 replication process and developing anti-EV71 drugs and vaccines. In this review, we summarized the recent progress in the studies of EV71 noncoding regions(50 UTR and 30 UTR) and all structural and nonstructural proteins, especially the key motifs involving in viral infection, replication, and immune regulation. This review will promote our understanding of EV71 virus replication and pathogenesis, and will facilitate the development of novel drugs or vaccines to treat EV71.展开更多
目的 通过检测餐饮从业人员、学生等体检人员中乙肝病毒大蛋白（Hepatitis B virus large proteins,HBV-LP）的情况,探讨乙肝病毒大蛋白用于公共体检领域的意义.方法 分别采用酶联免疫法（ELISA）测定乙肝两对半（HBV-M）以及乙型肝炎...目的 通过检测餐饮从业人员、学生等体检人员中乙肝病毒大蛋白（Hepatitis B virus large proteins,HBV-LP）的情况,探讨乙肝病毒大蛋白用于公共体检领域的意义.方法 分别采用酶联免疫法（ELISA）测定乙肝两对半（HBV-M）以及乙型肝炎病毒大蛋白（HBV-LP）,采用real-time PCR法检测HBV-DNA,对各类体检人员的709份标本分别进行检测.结果 （1）HBV-LP法与HBV-DNA 法检测709例中HBsAg阳性的体检人员的检测结果 差异无统计学意义,x2值和P值分别为1.47和0.23.（2）不同乙肝两对半模式中HBV-LP法与HBV-DNA法的检测结果 差异均无统计学意义,x2值和P值分别为2.67,0.60,0.00和0.10,0.44,1.00;（3）HBV-LP S/A值与HBV-DNA的拷贝数呈正相关性（r=0.959,P＜0.05）.结论 乙型肝炎病毒大蛋白（HBV-LP）能够有效、真实地反映HBV病毒复制情况,可以广泛应用于公共体检领域.展开更多
IFN-α/β是机体对抗外来病原体的第一道防线,其产生和后续激活的细胞信号转导可诱导具有抑制病毒感染和复制作用的IFN刺激基因的表达。然而,大多数病毒可通过表达一种或多种蛋白抵抗宿主细胞的抗病毒反应。流感病毒非结构蛋白1(nonstru...IFN-α/β是机体对抗外来病原体的第一道防线,其产生和后续激活的细胞信号转导可诱导具有抑制病毒感染和复制作用的IFN刺激基因的表达。然而,大多数病毒可通过表达一种或多种蛋白抵抗宿主细胞的抗病毒反应。流感病毒非结构蛋白1(nonstructural protein 1,NS1)作为多功能毒力因子,在流感病毒感染过程中起重要作用。此文概述了NS1的结构与功能及抑制IFN-α/β产生和抗病毒效应作用的机制,为研究流感病毒致病机理提供参考。展开更多
基金supported by the National Natural Science Foundation of China (Grant 81503118)CAMS Initiative for Innovative Medicine (CAMS-I2 M-1-010)The National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX09711003-005-004)
文摘Enterovirus 71(EV71) is one of the main pathogens that causes hand-foot-and-mouth disease(HFMD). HFMD caused by EV71 infection is mostly self-limited; however, some infections can cause severe neurological diseases, such as aseptic meningitis, brain stem encephalitis, and even death. There are still no effective clinical drugs used for the prevention and treatment of HFMD. Studying EV71 protein function is essential for elucidating the EV71 replication process and developing anti-EV71 drugs and vaccines. In this review, we summarized the recent progress in the studies of EV71 noncoding regions(50 UTR and 30 UTR) and all structural and nonstructural proteins, especially the key motifs involving in viral infection, replication, and immune regulation. This review will promote our understanding of EV71 virus replication and pathogenesis, and will facilitate the development of novel drugs or vaccines to treat EV71.
文摘IFN-α/β是机体对抗外来病原体的第一道防线,其产生和后续激活的细胞信号转导可诱导具有抑制病毒感染和复制作用的IFN刺激基因的表达。然而,大多数病毒可通过表达一种或多种蛋白抵抗宿主细胞的抗病毒反应。流感病毒非结构蛋白1(nonstructural protein 1,NS1)作为多功能毒力因子,在流感病毒感染过程中起重要作用。此文概述了NS1的结构与功能及抑制IFN-α/β产生和抗病毒效应作用的机制,为研究流感病毒致病机理提供参考。
