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Effects of different LEDs light spectrum on the growth,leaf anatomy,and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse 被引量:5
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作者 CHEN Li-li ZHANG Kai +5 位作者 GONG Xiao-chen WANG Hao-ying GAO You-hui WANG Xi-quan ZENG Zhao-hai HU Yue-gao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第1期108-119,共12页
Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the m... Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives. 展开更多
关键词 potato plantlets in vitro LEDs light spectrum leaf anatomy chloroplast ultrastructure minituber production
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Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro 被引量:4
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作者 CHEN Li-li WANG Hao-ying +3 位作者 GONG Xiao-chen ZENG Zhao-hai XUE Xu-zhang HU Yue-gao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第11期2914-2931,共18页
Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ... Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect. 展开更多
关键词 potato(Solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone
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Effects of Sion Cold Resistance of Cymbidium Plantletsin vitro
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作者 DUAN Xiaoyu TANG Min WANG Ling 《Journal of Landscape Research》 2013年第4期35-36,40,共3页
To determine the medium with the concentration of Na2SiO3 , the effect of different concentrations of Na2SiO3 on cold resistance of Cymbidium plantlets in vitro was studied. The results showed that Si enhanced osmotic... To determine the medium with the concentration of Na2SiO3 , the effect of different concentrations of Na2SiO3 on cold resistance of Cymbidium plantlets in vitro was studied. The results showed that Si enhanced osmotic regulation ability of Cymbidium evidently on cold resistance with increasing contents of free Pro, soluble sugar, soluble protein and decreasing contents of MDA. Compared with CK, osmotic regulation substances are accumulated rapidly. Free Pro, soluble sugar, soluble protein, MDA could be adopted as reference indices to evaluate capability of frost resistance of Cymbidium. By experiment we stated Na2SiO3 (3mmol/L) could relieve low temperature injury of Cymbidium plantlets in vitro. Mitigative effect was reduced with increasing concentration of Na2SiO3 . 展开更多
关键词 NA2SIO3 CYMBIDIUM plantlets in vitro Low temperature stress
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Influence of Watering Solution and Phenotype on the Growth of <i>in Vitro</i>Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization
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作者 Arnaud Agbidinoukoun Gaston C. Ouikoun +7 位作者 Toussaint Mikpon Gatien T. Kamade Bienvenu T. Badou Richmy C. B. Aïsso Serge S. Houedjissin Jerome A. Houngue René C. Dossoukpevi Corneille Ahanhanzo 《Agricultural Sciences》 2021年第11期1215-1230,共16页
Pineapple is the first fruit crop cultivated in south Benin that greatly contributes for food and nutritional security and farmers’ income. But the lack of homogenous planting material constitutes the major constrain... Pineapple is the first fruit crop cultivated in south Benin that greatly contributes for food and nutritional security and farmers’ income. But the lack of homogenous planting material constitutes the major constraint for improving pineapple yield. <em>In vitro</em> micropropagation is now used in the production of homogenous and free disease planting materials of pineapple. However, the acclimatization to natural condition of pineapple plantlets is an important step in planting material production of this crop. Here, we determined the intrinsic and extrinsic factors which influence the behavior of plantlets during the acclimatization process. For this purpose, plantlets from different categories were selected, trimmed and planted on a horticultural substrate made up of potting soil, white sawdust and compost previously sterilized. The plantlets were then incubated in under acclimatization greenhouse with average temperature of 29<span style="white-space:nowrap;">&#730;</span>C and 70.2% of humidity. A batch of plantlets was subjected to two different watering solutions: Shive and Robbins solution and NPK 14-6-5 foliar fertilizer. The results obtained initially showed high rate (100%) of survival and growth of the plantlets watered with Shive and Robbins solution against 50% of the plantlets watered with the foliar fertilizer solution. In addition, the plantlets with spread pores exhibiting the characteristics of which the number of leaves varies between 9 and 11, the weight between 1.2 and 1.5 g, the size of 4.5 to 5.5 cm, and a good junction between the aerial part and the root system were those which were successfully grown in acclimatization phase under greenhouse, unlike plantlets with erected pores having lower success rate. This study goes a long way in providing good procedures of acclimatization of homogenous and free disease planting material of pineapple to the famers. 展开更多
关键词 Ananas comosus var. comosus plantlets Morphology Nutrient Supply Pineapple Acclimatization In vitro Tissue Culture
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Effects of Lanthanum and Europium on Rooting of Plantlet Eriobotrya Japonica Lindl. in vitro 被引量:5
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作者 宋卫平 洪法水 +1 位作者 万志刚 周玉珍 《Journal of Rare Earths》 SCIE EI CAS CSCD 2002年第6期658-662,共5页
The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the lengt... The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0~3.0 μmol·L -1), Eu 3+ (2.0~3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+. 展开更多
关键词 rare earths LANTHANUM EUROPIUM Eriobotrya japonica Lindl plantlet in vitro ROOTING
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Effects of Neodymium on Nitrogen Metabolism of Plantlet Loquat in Vitro
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作者 万志刚 宋卫平 +4 位作者 俞明亮 洪法水 顾福根 苏国兴 赵密珍 《Journal of Rare Earths》 SCIE EI CAS CSCD 2004年第3期431-435,共5页
The effect of NdCl_3 on the rooting and nitrogen metabolism of loquat in vitro was studied when NdCl_3 was added to the rooted medium. The results show that 0.4 μmol·L^(-1) NdCl_3 in the rooted medium can obviou... The effect of NdCl_3 on the rooting and nitrogen metabolism of loquat in vitro was studied when NdCl_3 was added to the rooted medium. The results show that 0.4 μmol·L^(-1) NdCl_3 in the rooted medium can obviously increase the rooting rate, length of root and fresh weight of roots, and enhance the activities of nitrate reductase, glutamine synthetase, glutamate dehydrogenase in the root system and in the leaves. The transformation of NO_3^- to NH_4^+ in root system and leaves are promoted and the nitrogen metabolism is accelerated with 0.4 μmol·L^(-1) NdCl_3 treatment. 展开更多
关键词 agricultural biology NEODYMIUM loquat plantlet in vitro ROOTING nitrogen metabolism rare earths
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Effect of Lanthanum on Deferring Root Ageing of Peach Plantlet in Vitro
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作者 宋卫平 陈鹏 +1 位作者 万志刚 洪法水 《Journal of Rare Earths》 SCIE EI CAS CSCD 2005年第S1期508-512,共5页
Poor adventitious root formation is a major obstacle in micropropagation and in conventional propagation. The effects of LaCl_3 on root growth and deferring the root ageing of peach plantlet in vitro were investigated... Poor adventitious root formation is a major obstacle in micropropagation and in conventional propagation. The effects of LaCl_3 on root growth and deferring the root ageing of peach plantlet in vitro were investigated. The results show that the optimum concentration of LaCl_3 (2.5 μmol·L -1) in the rooted medium can significantly increase the rooting rate, the root length and the fresh weight, promote the activities of superoxide dismutase (SOD); catalase (CAT) and peroxidase (POD) and decrease the production rate of O_2 ·-, the malondialdehyde (MDA) contents and the plasma membrane permeability. There are important theory meaning and practical value in applying LaCl_3 in the rooting medium to raise the rate of rooting and transplant for stone fruit tree. 展开更多
关键词 LANTHANUM peach plantlet in vitro root ageing antioxidase activity rare earths
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Effects of Lanthanum on Root Growth and Senescence of GF_(43) (Prunus domestica) Plantlet in Vitro
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作者 宋卫平 顾福根 +3 位作者 于佳 沈叶 溪群华 刘佳佳 《Journal of Rare Earths》 SCIE EI CAS CSCD 2004年第5期696-700,共5页
The effect and the action mechanism of lanthanum on GF_(43) plantlet in vitro were studied. The results of experiments show that root growth rate and dry weight of GF_(43) by LaCl_3 treatments increase. The activities... The effect and the action mechanism of lanthanum on GF_(43) plantlet in vitro were studied. The results of experiments show that root growth rate and dry weight of GF_(43) by LaCl_3 treatments increase. The activities of antioxidant enzymes in root system such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) apparently enhance. Moreover ·O^-_2 and malond ialdehyde (MDA) contents and cell membrane permeability of GF_(43) are decreased by LaCl_3. The relatively stable membrane structure of cell could also be maintained and the root ageing of GF_(43) plantlet in vitro delays. 展开更多
关键词 agricultural biology LaCl_3 GF_(43) plantlet in vitro ROOTING SENESCENCE rare earths
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A rapid multiplication system for'Candidatus Liberibacter asiaticus'through regeneration of axillary buds in vitro 被引量:2
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作者 LEI Tian-gang HE Yong-rui +7 位作者 ZOU Xiu-ping WANG Xue-feng FU Shi-min PENG Ai-hong XU Lan-zhen YAO Li-xiao CHEN Shan-chun ZHOU Chang-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第6期1683-1693,共11页
‘Candidatus Liberibacter asiaticus(CLas)’,which causes citrus Huanglongbing(HLB)disease,has not been successfully cultured in vitro to date.Here,a rapid multiplication system for CLas was established through in vitr... ‘Candidatus Liberibacter asiaticus(CLas)’,which causes citrus Huanglongbing(HLB)disease,has not been successfully cultured in vitro to date.Here,a rapid multiplication system for CLas was established through in vitro regeneration of axillary buds from CLas-infected‘Changyecheng’sweet orange(Citrus sinensis Osbeck).Stem segments with a single axillary bud were cultured in vitro to allow CLas to multiply in the regenerating axillary buds.A high CLas titer was detected in the regenerated shoots on an optimized medium at 30 days after germination(DAG).This titer was 28.2-fold higher than in the midribs from CLas-infected trees growing in the greenhouse.To minimize contamination during in vitro regeneration,CLas-infected axillary buds were micrografted onto seedlings of‘Changyecheng’sweet orange and cultured in a liquid medium.In this culture,the titers of CLas in regenerated shoots rapidly increased from 7.5×10^(4)to 1.4×10^(8)cellsμg^(-1)of citrus DNA during the first 40 DAG.The percentages of shoots with>1×10^(8)CLas cellsμg^(-1)DNA were 30 and 40%at 30 and 40 DAG,respectively.Direct tissue blot immunoassay(DTBIA)indicated that the distribution of CLas was much more uniform in regenerated plantlets than in CLas-infected trees growing in the greenhouse.The disease symptoms in the plantlets were die-back,stunted growth,leaf necrosis/yellowing,and defoliation.The death rate of the plantlets was 82.0%at 60 DAG.Our results show that CLas can effectively multiply in citrus plantlests in vitro.This method will be useful for studying plant-HLB interactions and for rapid screening of therapeutic compounds against CLas in citrus. 展开更多
关键词 CITRUS ‘Candidatus Liberibacter asiaticus’ MULTIPLICATION in vitro citrus plantlets
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LED光源不同光质比例对红掌试管苗生长的影响 被引量:39
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作者 陈颖 王政 +2 位作者 纪思羽 何松林 夏玉玲 《江西农业大学学报》 CAS CSCD 北大核心 2013年第2期375-380,共6页
以红掌品种"骄阳"试管苗为材料,研究LED光源不同光质比例对红掌试管苗生长的影响。结果表明:在70%R(红光)+30%B(蓝光)处理下,红掌试管苗的株高、叶数、干鲜质量、根系活力达到最大值;在80%R+20%B处理下,红掌试管苗的根数、根... 以红掌品种"骄阳"试管苗为材料,研究LED光源不同光质比例对红掌试管苗生长的影响。结果表明:在70%R(红光)+30%B(蓝光)处理下,红掌试管苗的株高、叶数、干鲜质量、根系活力达到最大值;在80%R+20%B处理下,红掌试管苗的根数、根长、可溶性糖含量达到最大值;在50%R+50%B处理下,红掌试管苗的叶长、叶幅达到最大值,且在此处理下叶绿素a、叶绿素a+b、叶绿素a/b均达到最大值;蓝光处理下有利于干物质积累。红蓝组合光处理的红掌试管苗较单一红蓝光有明显优势,在50%R+50%B处理下有利于红掌试管苗的形态建成,其根系活力、可溶性糖含量均显著高于对照,是适宜红掌试管苗生长的最佳红蓝光配比。试验结果表明LED光源对促进红掌试管苗的生长和提高其品质效果明显。 展开更多
关键词 LED 光质 红掌 试管苗
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不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响 被引量:57
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作者 尚文倩 王政 +2 位作者 侯甲男 郑卫杰 何松林 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2013年第5期155-159,共5页
【目的】研究不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响,为高品质的铁皮石斛试管苗生产提供理论依据,也为LED光源在植物组培中的应用和经济可行性分析提供参考。【方法】以铁皮石斛试管苗为试材,在LED光源的红光100%R(主波长640... 【目的】研究不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响,为高品质的铁皮石斛试管苗生产提供理论依据,也为LED光源在植物组培中的应用和经济可行性分析提供参考。【方法】以铁皮石斛试管苗为试材,在LED光源的红光100%R(主波长640nm),蓝光100%B(主波长464nm),80%R+20%B,70%R+30%B,50%R+50%B 5种不同光质配比下培养,以普通荧光灯(PGFL)作为对照,比较经不同光质比处理90d后试管苗的形态指标、叶绿素、可溶性糖、可溶性蛋白含量以及根系活力。【结果】铁皮石斛试管苗株高在100%R处理下达到最大值;叶数、根长均在80%R+20%B处理下达到最大值;根数在70%R+30%B处理下达到最大值。在50%R+50%B处理下,铁皮石斛试管苗的干鲜质量、叶绿素a、叶绿素b、叶绿素(a+b)、可溶性糖含量和根系活力均达到最大值。可溶性蛋白在100%B处理下达到最大值。【结论】红蓝光比例为1∶1时有利于铁皮石斛试管苗的生长、叶绿素合成及干物质和糖的积累。 展开更多
关键词 LED 红蓝光 铁皮石斛 试管苗
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草莓试管苗移栽基质研究 被引量:16
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作者 王连润 杨松光 +2 位作者 胡忠荣 李坤明 马钧 《西南农业学报》 CSCD 北大核心 2010年第4期1374-1376,共3页
通过对草莓试管苗进行不同移栽基质试验,研究了不同移栽基质对草莓试管苗生长的影响。结果表明,草莓试管苗的最佳移栽基质是腐殖土∶红土=1∶1,移栽成活率近90%。
关键词 草莓 试管苗 移栽
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秋水仙素对大蒜茎尖试管苗四倍体的诱导 被引量:10
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作者 孔素萍 曹齐卫 +2 位作者 孙敬强 刘波 徐坤 《中国农业科学》 CAS CSCD 北大核心 2014年第15期3025-3033,共9页
【目的】大蒜为无性繁殖作物,难以采用常规育种方法选育新品种,而化学诱变作为种质创制的重要方法之一,在一些作物上获得了较好的效果。探讨秋水仙素对大蒜茎尖组培试管苗多倍体的诱变效应,旨在为创新大蒜种质提供理论依据和技术方法。... 【目的】大蒜为无性繁殖作物,难以采用常规育种方法选育新品种,而化学诱变作为种质创制的重要方法之一,在一些作物上获得了较好的效果。探讨秋水仙素对大蒜茎尖组培试管苗多倍体的诱变效应,旨在为创新大蒜种质提供理论依据和技术方法。【方法】选用山东‘苍山蒲棵’、四川‘正月早’和‘新疆紫皮’3个生态区域的大蒜品种,鳞茎经自然晾干通过休眠后,取鳞芽茎尖为外植体接种到不同处理的培养基上,采用二次饱和-D最优设计方法,研究秋水仙素不同浓度及处理时间对大蒜茎尖试管苗成苗率及染色体加倍率的影响,并建立相关回归模型;通过对回归模型进行解析,确定诱变大蒜四倍体的优化方案;采用流式细胞仪检测幼叶和根尖压片染色体计数相结合的方法进行染色体倍性鉴定。【结果】利用流式细胞仪检测的四倍体大蒜植株叶片DNA相对含量是二倍体对照大蒜‘苍山蒲棵’的2倍;通过染色体计数法鉴定二倍体植株根尖染色体为2n=2x=16,而四倍体植株染色体为2n=4x=32。在秋水仙素处理大蒜茎尖诱变四倍体过程中,随秋水仙素处理浓度的升高及时间的延长,3个大蒜品种试管苗成苗率均逐渐降低,且处理浓度的影响大于处理时间;品种间成苗率存在显著差异,以‘正月早’较高,‘新疆紫皮’次之,‘苍山蒲棵’较低,其处理间平均成苗率分别为48.69%、46.73%和41.83%。秋水仙素对大蒜四倍体的诱变效应以处理浓度大于处理时间,不同大蒜品种对秋水仙素的反应存在显著差异,虽然‘苍山蒲棵’、‘正月早’与‘新疆紫皮’的最高四倍体诱导率分别达15.69%、15.69%和23.53%,但大蒜四倍体平均诱导率则以‘正月早’最高,‘苍山蒲棵’次之,‘新疆紫皮’最低,分别为9.15%、7.84%和6.53%。经计算机模拟寻优,求得的3个大蒜品种试管苗成苗率均超过50%的处理方案为秋水仙素浓度为0.268%—0.694%、处理时间为61.1—113.8 h;3个大蒜品种四倍体诱导率均超过5%的秋水仙素处理浓度和时间分别为0.531%—0.580%和79.8—112.2 h。【结论】不同大蒜品种对秋水仙素处理浓度和处理时间的反应不同,秋水仙素对大蒜四倍体的诱变效应亦存在显著差异,但均以处理浓度的影响大于处理时间,且处理浓度与处理时间存在互作效应。3个大蒜品种成苗率超过50%且四倍体率超过5%的秋水仙素处理浓度为0.531%—0.580%、处理时间为79.8—112.2 h。 展开更多
关键词 大蒜 试管苗 秋水仙素 四倍体
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植物试管苗离体生根的研究进展 被引量:42
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作者 李胜 杨德龙 +2 位作者 李唯 武季玲 曹孜义 《甘肃农业大学学报》 CAS CSCD 2003年第4期373-384,共12页
综述了植物试管苗生根机理方面的基因表达和遗传转化,内源激素和酶类活性与生根的关系;培养基组成如矿物盐类、附加有机成分(维生素和氨基酸)、生长调节物质、蔗糖、琼脂、活性碳、酚类物质,以及pH值、通气、湿度、温度和光照等培养条件... 综述了植物试管苗生根机理方面的基因表达和遗传转化,内源激素和酶类活性与生根的关系;培养基组成如矿物盐类、附加有机成分(维生素和氨基酸)、生长调节物质、蔗糖、琼脂、活性碳、酚类物质,以及pH值、通气、湿度、温度和光照等培养条件,对试管苗生根的影响;外植体基因型与插条生理和发育状态与生根的关系。 展开更多
关键词 试管苗 离体生根 综述
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葡萄试管苗土支撑培养带坨移栽研究 被引量:21
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作者 柴慈江 严仁玲 +2 位作者 王震星 孟庆田 杨恩芹 《华北农学报》 CSCD 北大核心 1995年第1期116-119,共4页
以土作为培养基支撑物,离体培养葡萄试管苗双节茎段,40天后即达到适于炼苗标准,成苗率95.8%。带坨移入营养钵,在不喷雾、不覆膜、空气湿度低至45%条件下,成活率达96.7%。成活的试管苗炼苗54天后移入大田,移栽成... 以土作为培养基支撑物,离体培养葡萄试管苗双节茎段,40天后即达到适于炼苗标准,成苗率95.8%。带坨移入营养钵,在不喷雾、不覆膜、空气湿度低至45%条件下,成活率达96.7%。成活的试管苗炼苗54天后移入大田,移栽成活率为100%,其中特级苗占87.5%,一级苗占12.5%。试验证明,土支撑培养带坨移栽技术是简化试管苗移栽程序、降低成本的有效措施。 展开更多
关键词 葡萄 试管苗 带坨移栽 土支撑培养
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豫杂一号泡桐体细胞同源四倍体诱导及其体外植株再生 被引量:24
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作者 范国强 翟晓巧 +1 位作者 魏真真 杨志清 《东北林业大学学报》 CAS CSCD 北大核心 2010年第12期22-26,共5页
在含不同质量浓度秋水仙素固液双层培养基上,以叶片为外植体进行了豫杂一号泡桐同源四倍体的诱导,同时建立了其体外植株再生系统。结果表明,秋水仙素质量浓度和处理时间对豫杂一号泡桐同源四倍体诱导率影响显著,外植体预培养时间影响不... 在含不同质量浓度秋水仙素固液双层培养基上,以叶片为外植体进行了豫杂一号泡桐同源四倍体的诱导,同时建立了其体外植株再生系统。结果表明,秋水仙素质量浓度和处理时间对豫杂一号泡桐同源四倍体诱导率影响显著,外植体预培养时间影响不显著。在含20 mg.L-1秋水仙素MS培养基上处理48 h,未经预培养叶片四倍体诱导率最高达21.2%。同源四倍体幼苗叶片及其单气孔器较二倍体变大,气孔密度变小,叶绿素含量和SOD活性升高,MDA含量和POD活性降低。此外,叶片是同源四倍体豫杂一号泡桐体外植株再生的最佳外植体,MS+0.5 mg.L-1NAA+16 mg.L-1BA和1/2MS+0.1 mg.L-1NAA分别是其芽诱导和根诱导的最适培养基。 展开更多
关键词 豫杂一号泡桐 秋水仙素 同源四倍体 诱导 体外植株再生
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不同培养方式对马铃薯试管苗生长与试管薯诱导的影响 被引量:17
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作者 白淑霞 安忠民 +1 位作者 王静 冯学赞 《中国生态农业学报》 CAS CSCD 2002年第2期40-41,共2页
应用4种不同的培养方式,分别就不同状态培养基、用量及添加时期对试管苗和试管薯的生长发育的影响研究结果表明,与固体培养基相比,采用液体培养基减量、分次添加使用方法试管苗各项生长指标均达极显著水平,茎粗增加0.89cm,单株有效节数... 应用4种不同的培养方式,分别就不同状态培养基、用量及添加时期对试管苗和试管薯的生长发育的影响研究结果表明,与固体培养基相比,采用液体培养基减量、分次添加使用方法试管苗各项生长指标均达极显著水平,茎粗增加0.89cm,单株有效节数增加2节,鲜物质量和干物质量分别提高了4.15g和0.27g,成苗天数减少5.5d。试管薯的结薯期提早2~3d,单株结薯数达到2粒,单薯重多为100~1000mg,且生产成本降低了72.42%。 展开更多
关键词 培养方式 马铃薯 试管苗 生长 试管薯诱导
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盐胁迫下水杨酸对南林895杨组培苗抗氧化系统的影响 被引量:34
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作者 陈颖 徐彩平 +3 位作者 汪南阳 胡菲 王光萍 黄敏仁 《南京林业大学学报(自然科学版)》 CAS CSCD 北大核心 2012年第6期17-22,共6页
以南林895杨组培苗为材料,研究了盐胁迫下水杨酸(SA)对其耐盐及抗氧化能力的影响。结果表明:与单独盐处理(100 mmol/L NaCl)相比,盐胁迫下添加SA(0.01、0.05、0.1 mmol/L)能显著地提高南林895杨苗的含水量和总生物量;显著提高根和叶中... 以南林895杨组培苗为材料,研究了盐胁迫下水杨酸(SA)对其耐盐及抗氧化能力的影响。结果表明:与单独盐处理(100 mmol/L NaCl)相比,盐胁迫下添加SA(0.01、0.05、0.1 mmol/L)能显著地提高南林895杨苗的含水量和总生物量;显著提高根和叶中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD,叶中除外)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等5种抗氧化酶活性和抗坏血酸(AsA)、还原型谷胱苷肽(GSH)两种抗氧化剂的含量,其中0.01和0.05 mmol/L SA处理对保护酶提高效果显著;而0.05和0.1 mmol/L SA处理显著地降低了根和叶中O.2-和H2O2的含量,且在根中降低的程度远大于叶片中,另外SA处理也显著降低了叶片中MDA的含量。总之,水杨酸主要通过提高南林895杨根和叶中保护酶活性、增加抗氧化剂含量来清除活性氧,根和叶中对活性氧的清除存在不同的机制,抗坏血酸-谷胱甘肽循环参与了叶片中H2O2的清除,而根中H2O2的清除主要由过氧化物酶和抗坏血酸过氧化物酶来完成。0.01~0.05 mmol/L的水杨酸可以有效缓解盐胁迫对南林895杨组培苗的伤害。 展开更多
关键词 水杨酸 盐胁迫 南林895杨组培苗 抗氧化系统
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栓皮栎茎段离体培养的研究 被引量:34
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作者 张存旭 宋敏 赵忠 《西北植物学报》 CAS CSCD 2004年第7期1260-1265,共6页
以4~6个月的实生苗茎段为外植体,研究了离体条件下培养基及激素对栓皮栎器官发生和植株再生的影响。结果表明:初代培养选用低盐培养基WPM、BTM和GD,附加0.2mg·L-16-BA,丛生芽较多,茎粗壮,生长势健壮,均好于高盐培养基MS。继代培养... 以4~6个月的实生苗茎段为外植体,研究了离体条件下培养基及激素对栓皮栎器官发生和植株再生的影响。结果表明:初代培养选用低盐培养基WPM、BTM和GD,附加0.2mg·L-16-BA,丛生芽较多,茎粗壮,生长势健壮,均好于高盐培养基MS。继代培养时,以BTM为基本培养基,添加0.2~0.6mg·L-16-BA有利于茎芽增殖和生长,当6-BA浓度为0.8~1.0mg·L-1时,不利于茎芽伸长。以WPM为基本培养基,附加NAA0.1mg·L-1和IBA0.25mg·L-1时,生根率高,根系发育好。 展开更多
关键词 离体培养 茎段 植株再生 栓皮栎
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胡杨离体器官发生及试管无性系的建立 被引量:31
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作者 蒋湘宁 谷瑞升 郭仲琛 《Acta Botanica Sinica》 SCIE CAS CSCD 1999年第1期29-33,共5页
研究了离体条件下胡杨(Populus euphratica Oliver)茎段、叶片及愈伤组织的器官发生和植株再生技术。离体培养以MS为基本培养基并附加40mg/L腺嘌呤和500mg/L水解乳蛋白。离体叶片和茎段在B... 研究了离体条件下胡杨(Populus euphratica Oliver)茎段、叶片及愈伤组织的器官发生和植株再生技术。离体培养以MS为基本培养基并附加40mg/L腺嘌呤和500mg/L水解乳蛋白。离体叶片和茎段在BA为0.5mg/L和NAA为0.5mg/L的培养基上诱导产生愈伤组织,并在含0.25mg/LBA和0.5mg/LNAA的培养基上继代增殖。BA为0.5mg/L和NAA为0.1mg/L可诱导叶片和愈伤组织发生不定芽,诱导频率分别为100%和82.9%,对于茎段,BA和NAA分别为0.1mg/L和0.01mg/L时诱导不定芽频率可达83%。试管苗在大量元素减半并附加0.015mg/LNAA的MS培养基上诱导生根,生根率达86.2%。 展开更多
关键词 胡杨 器官发生 再生植株
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