[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials...[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA （with addition of 0.1 mg/L NAA）, fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS ＋ BA 1.2 mg/L ＋ NAA 0. 1 mg/L ＋ sucrose 30 g/L ＋ agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.展开更多
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi...Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.展开更多
Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applica...Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applications such as cell therapy, tissue engineering, regenerative medicine and drug screening. However, the clinical applications of SCs require a large amount of SCs with high quality and the number of SCs from their tissue resources is very limited. Large-scale expansion is needed to generate homogeneous SCs with good biological characteristics for clinical application. This necessitates a bioreactor system to provide controllable and stable conditions for stem cell (SC) culture. Traditional methods of bioreactor for maintenance and expansion of cells rely on two-dimensional (2-D) culture techniques, leading to loss self-renewal ability and differentiation capacity upon long-term culture. New approaches for SC expansion with bioreactor employ three-dimensional (3-D) cell growth to mimic their environment in vivo. In this review, we summarize the application of bioreactors in SC culture.展开更多
This paper investigates the socio-cultural challenges of the small-scale batik and tie-dye production and retailing industry in Ghana. The research is based on a qualitative research approach that employs interviews a...This paper investigates the socio-cultural challenges of the small-scale batik and tie-dye production and retailing industry in Ghana. The research is based on a qualitative research approach that employs interviews and observation for data collection. In all, sixteen (16) participants were sampled through purposive techniques. Data collected were transcribed, classified and analysed through thematic and descriptive analysis. The study found that family roles contradict the business role, pressure from family members, poor support from society members, some religious teachings, age, ethnicity and lifestyle of certain people among others. The study concludes that socio-cultural challenges have led to the collapse of most batik and tie-dye production and retailing sectors leaving thousands of Ghanaians jobless. It recommends among others that producers and retailers of batik and tie-dye must separate family roles from work responsibilities to enable them to perform effectively. Again, family members, parents and peers must desist from putting pressure on the entrepreneurs for freebies and the government must resource NBSSI, GEPA and GSB to have regular in-service training to batik and tie-dye producers and retailers to address poor financial management and technical challenges and also on product quality on the international market.展开更多
[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, ...[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.展开更多
With the introduction of pragmatic studies, politeness has aroused great interest among Chinese scholars. Different cultures have different traditions and values, which bring out different understanding and applicatio...With the introduction of pragmatic studies, politeness has aroused great interest among Chinese scholars. Different cultures have different traditions and values, which bring out different understanding and application of politeness strategies. Great Chinese culture brings out the complicated Chinese politeness scale. A successful communication depends on politeness principle. Learning the Chinese politeness scale will make we know more about the Chinese culture. By researching this, we will perform better in communication.展开更多
Globalization makes people from diverse cultural backgrounds communicate effectively. To be a global citizen has become the goal of our education. Landis and Bhagat （1996） argued that intercultural sensitivity is cr...Globalization makes people from diverse cultural backgrounds communicate effectively. To be a global citizen has become the goal of our education. Landis and Bhagat （1996） argued that intercultural sensitivity is crucial to enable people to live and work with others from different cultural backgrounds. With higher intercultural sensitivity, one will be more global as his understanding of cultural difference becomes more sophisticated. In this present study, the intercultural sensitivity of different majors from a private institute of technology in the north of Taiwan was investigated, using the ISS （Intercultural Sensitivity Scale） developed by Chen and Starosta （2000）. Based on a survey of 358 participants from Nursing Department and Department of Nutrition and Health Sciences, this study focused on five dimensions of the intercultural sensitivity and the participants＇ demographic factors affecting their performance on intercultural sensitivity. The five dimensions included in this study were： （1） interaction engagement, （2） interaction confidence, （3） respect for cultural differences, （4） interaction enjoyment, and （5） interaction attentiveness. Among the five dimensions of intercultural sensitivity, the participants had the highest score on the dimension of ＂respect for the cultural differences＂ and had the lowest score on the dimension of ＂interaction confidence＂. Results generated by stepwise multiple regression indicated ＂decision of studying abroad＂ and ＂experiences of living abroad＂ could effectively predict the participants＇ intercultural sensitivity.展开更多
The paper reviews previous publications and reports some comments about a semi empirical model of the growth and decay process of a planktonic microbial culture. After summarizing and reshaping some fundamental mathem...The paper reviews previous publications and reports some comments about a semi empirical model of the growth and decay process of a planktonic microbial culture. After summarizing and reshaping some fundamental mathematical expressions, the paper highlights the reasons for the choice of a suitable time origin that makes the parameters of the model self-consistent. Besides the potential applications to predictive microbiology studies and to effects of bactericidal drugs, the model allows a suitable proxy of the fitness of the microbial culture, which can be of interest for the studies on the evolution across some thousand generations of a Long Term Evolution Experiment.展开更多
文摘[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA （with addition of 0.1 mg/L NAA）, fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS ＋ BA 1.2 mg/L ＋ NAA 0. 1 mg/L ＋ sucrose 30 g/L ＋ agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.
文摘Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.
文摘Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applications such as cell therapy, tissue engineering, regenerative medicine and drug screening. However, the clinical applications of SCs require a large amount of SCs with high quality and the number of SCs from their tissue resources is very limited. Large-scale expansion is needed to generate homogeneous SCs with good biological characteristics for clinical application. This necessitates a bioreactor system to provide controllable and stable conditions for stem cell (SC) culture. Traditional methods of bioreactor for maintenance and expansion of cells rely on two-dimensional (2-D) culture techniques, leading to loss self-renewal ability and differentiation capacity upon long-term culture. New approaches for SC expansion with bioreactor employ three-dimensional (3-D) cell growth to mimic their environment in vivo. In this review, we summarize the application of bioreactors in SC culture.
文摘This paper investigates the socio-cultural challenges of the small-scale batik and tie-dye production and retailing industry in Ghana. The research is based on a qualitative research approach that employs interviews and observation for data collection. In all, sixteen (16) participants were sampled through purposive techniques. Data collected were transcribed, classified and analysed through thematic and descriptive analysis. The study found that family roles contradict the business role, pressure from family members, poor support from society members, some religious teachings, age, ethnicity and lifestyle of certain people among others. The study concludes that socio-cultural challenges have led to the collapse of most batik and tie-dye production and retailing sectors leaving thousands of Ghanaians jobless. It recommends among others that producers and retailers of batik and tie-dye must separate family roles from work responsibilities to enable them to perform effectively. Again, family members, parents and peers must desist from putting pressure on the entrepreneurs for freebies and the government must resource NBSSI, GEPA and GSB to have regular in-service training to batik and tie-dye producers and retailers to address poor financial management and technical challenges and also on product quality on the international market.
基金Supported by Special Fund for the Dominant Subjects Development of the Higher Education Institutions of Jiangsu ProvinceScience and Technology Innovation Fund of Nanjing Forestry University(X09-120-4)~~
文摘[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.
文摘With the introduction of pragmatic studies, politeness has aroused great interest among Chinese scholars. Different cultures have different traditions and values, which bring out different understanding and application of politeness strategies. Great Chinese culture brings out the complicated Chinese politeness scale. A successful communication depends on politeness principle. Learning the Chinese politeness scale will make we know more about the Chinese culture. By researching this, we will perform better in communication.
文摘Globalization makes people from diverse cultural backgrounds communicate effectively. To be a global citizen has become the goal of our education. Landis and Bhagat （1996） argued that intercultural sensitivity is crucial to enable people to live and work with others from different cultural backgrounds. With higher intercultural sensitivity, one will be more global as his understanding of cultural difference becomes more sophisticated. In this present study, the intercultural sensitivity of different majors from a private institute of technology in the north of Taiwan was investigated, using the ISS （Intercultural Sensitivity Scale） developed by Chen and Starosta （2000）. Based on a survey of 358 participants from Nursing Department and Department of Nutrition and Health Sciences, this study focused on five dimensions of the intercultural sensitivity and the participants＇ demographic factors affecting their performance on intercultural sensitivity. The five dimensions included in this study were： （1） interaction engagement, （2） interaction confidence, （3） respect for cultural differences, （4） interaction enjoyment, and （5） interaction attentiveness. Among the five dimensions of intercultural sensitivity, the participants had the highest score on the dimension of ＂respect for the cultural differences＂ and had the lowest score on the dimension of ＂interaction confidence＂. Results generated by stepwise multiple regression indicated ＂decision of studying abroad＂ and ＂experiences of living abroad＂ could effectively predict the participants＇ intercultural sensitivity.
文摘The paper reviews previous publications and reports some comments about a semi empirical model of the growth and decay process of a planktonic microbial culture. After summarizing and reshaping some fundamental mathematical expressions, the paper highlights the reasons for the choice of a suitable time origin that makes the parameters of the model self-consistent. Besides the potential applications to predictive microbiology studies and to effects of bactericidal drugs, the model allows a suitable proxy of the fitness of the microbial culture, which can be of interest for the studies on the evolution across some thousand generations of a Long Term Evolution Experiment.
