CAEFusion: A New Convolutional Autoencoder-Based Infrared and Visible Light Image Fusion Algorithm

To address the issues of incomplete information,blurred details,loss of details,and insufficient contrast in infrared and visible image fusion,an image fusion algorithm based on a convolutional autoencoder is proposed.The region attention module is meant to extract the background feature map based on the distinct properties of the background feature map and the detail feature map.A multi-scale convolution attention module is suggested to enhance the communication of feature information.At the same time,the feature transformation module is introduced to learn more robust feature representations,aiming to preserve the integrity of image information.This study uses three available datasets from TNO,FLIR,and NIR to perform thorough quantitative and qualitative trials with five additional algorithms.The methods are assessed based on four indicators:information entropy(EN),standard deviation(SD),spatial frequency(SF),and average gradient(AG).Object detection experiments were done on the M3FD dataset to further verify the algorithm’s performance in comparison with five other algorithms.The algorithm’s accuracy was evaluated using the mean average precision at a threshold of 0.5(mAP@0.5)index.Comprehensive experimental findings show that CAEFusion performs well in subjective visual and objective evaluation criteria and has promising potential in downstream object detection tasks.

Infrared and Visible Image Fusion Based on Res2Net-Transformer Automatic Encoding and Decoding

A novel image fusion network framework with an autonomous encoder and decoder is suggested to increase thevisual impression of fused images by improving the quality of infrared and visible light picture fusion. The networkcomprises an encoder module, fusion layer, decoder module, and edge improvementmodule. The encoder moduleutilizes an enhanced Inception module for shallow feature extraction, then combines Res2Net and Transformerto achieve deep-level co-extraction of local and global features from the original picture. An edge enhancementmodule (EEM) is created to extract significant edge features. A modal maximum difference fusion strategy isintroduced to enhance the adaptive representation of information in various regions of the source image, therebyenhancing the contrast of the fused image. The encoder and the EEM module extract features, which are thencombined in the fusion layer to create a fused picture using the decoder. Three datasets were chosen to test thealgorithmproposed in this paper. The results of the experiments demonstrate that the network effectively preservesbackground and detail information in both infrared and visible images, yielding superior outcomes in subjectiveand objective evaluations.

In situ infrared, Raman and X-ray spectroscopy for the mechanistic understanding of hydrogen evolution reaction

Hydrogen production by water reduction reactions has received considerable attention because hydrogen is considered a clean-energy carrier,key for a sustainable energy future.Computational methods have been widely used to study the reaction mechanism of the hydrogen evolution reaction(HER),but the calculation results need to be supported by experimental results and direct evidence to confirm the mechanistic insights.In this review,we discuss the fundamental principles of the in situ spectroscopic strategy and a theoretical model for a mechanistic understanding of the HER.In addition,we investigate recent studies by in situ Fourier transform infrared(FTIR),Raman spectroscopy,and X-ray absorption spectroscopy(XAS) and cover new findings that occur at the catalyst-electrolyte interface during HER.These spectroscopic strategies provide practical ways to elucidate catalyst phase,reaction intermediate,catalyst-electrolyte interface,intermediate binding energy,metal valency state,and coordination environment during HER.

Application of near-infrared spectroscopy for fast germplasm analysis and classification in multi-environment using intact-seed peanut(Arachis hypogaea L.)

Peanut is a worldwide oilseed crop and the need to assess germplasm in a non-destructive manner is important for seed nutritional breeding.In this study,Near Infrared Spectroscopy(NIRS)was applied to rapidly assess germplasm variability from whole seed of 699 samples,field-collected and assembled in four genetic and environmentbased sets:one set of 300 varieties of a core-collection and three sets of 133 genotypes of an interspecific population,evaluated in three environments in a large spatial scale of two countries,Mbalmayo and Bafia in Cameroon and Nioro in Senegal,under rainfed conditions.NIR elemental spectra were gathered on six subsets of seeds of each sample,after three rotation scans,with a spectral resolution of 16 cm-1over the spectral range of867 nm to 2530 nm.Spectra were then processed by principal component analysis(PCA)coupled with Partial least squares-discriminant analysis(PLS-DA).As results,a huge variability was found between varieties and genotypes for all NIR wavelength within and between environments.The magnitude of genetic variation was particularly observed at 11 relevant wavelengths such as 1723 nm,usually related to oil content and fatty acid composition.PCA yielded the most chemical attributes in three significant PCs(i.e.,eigenvalues>10),which together captured 93%of the total variation,revealing genetic and environment structure of varieties and genotypes into four clusters,corresponding to the four samples sets.The pattern of genetic variability of the interspecific population covers,remarkably half of spectrum of the core-collection,turning out to be the largest.Interestingly,a PLS-DA model was developed and a strong accuracy of 99.6%was achieved for the four sets,aiming to classify each seed sample according to environment origin.The confusion matrix achieved for the two sets of Bafia and Nioro showed 100%of instances classified correctly with 100%at both sensitivity and specificity,confirming that their seed quality was different from each other and all other samples.Overall,NIRS chemometrics is useful to assess and distinguish seeds from different environments and highlights the value of the interspecific population and core-collection,as a source of nutritional diversity,to support the breeding efforts.

Near Infrared Spectroscopy (NIRS) Model-Based Prediction for Protein Content in Cowpea

Cowpea (Vigna unguiculata L. Walp) is a multi-purpose legume with high quality protein for human consumption and livestock. The objective of this work was to develop near-infrared spectroscopy (NIRS) prediction models to estimate protein content in cowpea. A total of 116 cowpea breeding lines with a wide range of protein contents (19.28 % to 32.04%) were selected to build the model using whole seed and ground seed samples. Partial least-squares discriminant analysis (PLS-DA) regression technique with different pre-treatments (derivatives, standard normal variate, and multiplicative scatter correction) were carried out to develop the protein prediction model. Results showed: 1) spectral plots of both the whole seed and ground seed showed higher spectral scatter at higher wavelengths (>1450 nm), 2) data pre-processing affects prediction accuracy for bot whole seed and ground seed samples, 3) prediction using ground seed samples (0.64 R2 0.85) is better than the whole seed (0.33 R2 0.78), and 4) the data pre-processing second derivative with standard normal variate has the best prediction (R2_whole seed = 0.78, R2_ground seed = 0.85). The results will be of interest in cowpea breeding programs aimed at improving total seed protein content.

Photoprotective Ability of Sunscreens against Ultraviolet, Visible Light and Near-Infrared Radiation

Despite the widespread prevalence of daily sunscreen usage, solar-induced skin damage continues to occur. We have previously reported that solar visible light and near-infrared, in addition to ultraviolet radiation, perform as aging factors and induce deleterious effects such as photoaging, vasodilation, muscle thinning, skin ptosis, photoimmunosupression and photocarcinogenesis. Despite this, most commonly used sunscreens only block ultraviolet radiation. To evaluate the complete solar-spectrum blocking ability of sunscreens produced by internationally well-known companies, a double-beam spectrophotometer was used to optically measure the transmission spectra. The spectrophotometer utilizes a unique, single monochromatic design covering a wavelength range of 240 to 2600 nm. Sunscreens (thickness, 0.1 mm, SPF50+, PA+++ or ++++) from internationally well-known companies blocked 78.8% - 99.9% of ultraviolet, 33.4% - 99.6% of visible light, and 27.0% - 76.4% of near-infrared. It can be concluded that while most commercially available sunscreens filter ultraviolet radiation, they are not effective at blocking visible light and near-infrared radiation. The results of this study imply that sunscreens that provide comprehensive photoprotection from ultraviolet through to near-infrared should be considered to prevent skin photodamage.

Photoprotective Ability of Colored Iron Oxides in Tinted Sunscreens against Ultraviolet, Visible Light and Near-Infrared Radiation

Solar-induced skin damage continues to pose a problem to human health worldwide, despite the widespread recommendation and use of sunscreens. We have previously reported that solar visible light and near-infrared also contribute to skin damage and photoageing. Most commonly recommended sunscreens are only effective throughout the UV spectrum, offering no protection from visible light and near-infrared. To evaluate the enhanced solar-spectrum blocking ability of iron oxides, a double-beam spectrophotometer was used to optically measure the transmission spectra. The spectrophotometer deploys a unique, single monochromatic design to detect wavelength penetration in the range of 240 to 2600 nm. The sample without iron oxide (control) blocked over 80% of ultraviolet-C and ultraviolet-B but did not block ultraviolet-A, visible light, or near-infrared wavelengths. The samples with yellow, and red iron oxide blocked over 90% ultraviolet, but did not block visible light and near-infrared effectively. The sample with black iron oxide blocked visible light, and near-infrared effectively compared with other samples with yellow, blue, and red iron oxide. The sample with red and black iron oxides, and the sample with yellow, blue, red, and black iron oxides blocked ultraviolet through to near-infrared. It can be concluded that dark colored iron oxide combinations are effective at blocking from ultraviolet through to visible light and near-infrared radiation. The results of this study may also suggest that biological colour of human skin and subcutaneous tissues are conserved for comprehensive photoprotection.

Novel Low Viscosity Zinc Oxide, Iron Oxides and Erioglaucine Sunscreen Potential to Protect from Ultraviolet, Visible Light and Near-Infrared Radiation

Despite the widespread recommendation and use of sunscreens and ultraviolet blocking materials, solar-induced skin damage and photoageing continues to pose a problem to human health worldwide. We have previously reported that solar visible light and near-infrared also contribute to skin damage and photo ageing. Most commonly recommended sunscreens are only effective throughout the UV spectrum, offering no protection from visible light and near-infrared. A possible solution could be to augment sunscreens with metal oxides which block visible light and near-infrared radiation. To evaluate the enhanced solar-spectrum blocking ability of novel low viscosity sunscreen containing zinc and iron oxides, a double-beam spectrophotometer was used to optically measure the transmission spectra. The spectrophotometer deploys a unique, single monochromatic design to detect wavelength penetration in the range of 240 to 2600 nm. The Sunscreen base without zinc oxide and iron oxides (control) blocked over 80% of ultraviolet-C and ultraviolet-B but did not block ultraviolet-A, visible light, or near-infrared. The novel low viscosity zinc oxide sample blocked almost over 90% ultraviolet, but did not block visible light and near-infrared sufficiently. However, the samples with the novel low viscosity zinc oxide, iron oxides and erioglaucine blocked almost over 90% of ultraviolet, visible light and near-infrared. It can be concluded that this novel combination of low viscosity zinc oxide, iron oxides and erioglaucine is effective at blocking ultraviolet, visible light and near-infrared radiation. The results of this study imply that sunscreens that provide comprehensive photoprotection from ultraviolet through to near-infrared should be adopted to prevent skin photodamage.

Visible and Near-Infrared Spectroscopy with Multi-Parameters Optimization of Savitzky-Golay Smoothing Applied to Rapid Analysis of Soil Cr Content of Pearl River Delta

Using visible and near-infrared (Vis-NIR) spectroscopy combined with partial least squares (PLS) regression, the rapid reagent-free analysis model for chromium (Cr) content in tideland reclamation soil in the Pearl River Delta, China was established. Based on Savitzky-Golay (SG) smoothing and PLS regression, a multi-parameters optimization platform (SG-PLS) covering 264 modes was constructed to select the appropriately spectral preprocessing mode. The optimal SG-PLS model was determined according to the prediction effect. The selected optimal parameters d, p, m and LV were 2, 6, 23 and 8, respectively. Using the validation samples that were not involved in modeling, the root mean square error (SEPV), relative root mean square error (R-SEPV) and correlation coefficients (RP, V) of prediction were 11.66 mg·kg-1, 10.7% and 0.722, respectively. The results indicated that the feasibility of using Vis-NIR spectroscopy combined with SG-PLS method to analyze soil Cr content. The constructed multi-parameters optimization platform with SG-PLS is expected to be applied to a wider field of analysis. The rapid detection method has important application values to large-scale agricultural production.

Visible and Near-Infrared Spectroscopic Discriminant Analysis Applied to Brand Identification of Wine

High-end wine brand is made through the use of high-quality grape variety and yeast strain, and through a unique process. Not only is it rich in nutrients, but also it has a unique taste and a fragrant scent. Brand identification of wine is difficult and complex because of high similarity. In this paper, visible and near-infrared (NIR) spectroscopy combined with partial least squares discriminant analysis (PLS-DA) was used to explore the feasibility of wine brand identification. Chilean Aoyo wine (2016 vintage) was selected as the identification brand (negative, 100 samples), and various other brands of wine were used as interference brands (positive, 373 samples). Samples of each type were randomly divided into the calibration, prediction and validation sets. For comparison, the PLS-DA models were established in three independent and two complex wavebands of visible (400 - 780 nm), short-NIR (780 - 1100 nm), long-NIR (1100 - 2498 nm), whole NIR (780 - 2498 nm) and whole scanning (400 - 2498 nm). In independent validation, the five models all achieved good discriminant effects. Among them, the visible region model achieved the best effect. The recognition-accuracy rates in validation of negative, positive and total samples achieved 100%, 95.6% and 97.5%, respectively. The results indicated the feasibility of wine brand identification with Vis-NIR spectroscopy.

Visible and Near-Infrared Spectroscopic Discriminant Analysis Applied to Identification of Soy Sauce Adulteration

The identification of soy sauce adulteration can avoid fraud, and protect the rights and interests of producers and consumers. Based on two measurement models (1 mm, 10 mm), the visible and near-infrared (Vis-NIR) spectroscopy combined with standard normal variate-partial least squares-discriminant analysis (SNV-PLS-DA) was used to establish the discriminant analysis models for adulterated and brewed soy sauces. Chubang soy sauce was selected as an identification brand (negative, 70). The adulteration samples (positive, 72) were prepared by mixing Chubang soy sauce and blended soy sauce with different adulteration rates. Among them, the “blended soy sauce” sample was concocted of salt water (NaCl), monosodium glutamate (C5H10NNaO5) and caramel color (C6H8O3). The rigorous calibration-prediction-validation sample design was adopted. For the case of 1 mm, five waveband models (visible, short-NIR, long-NIR, whole NIR and whole scanning regions) were established respectively;in the case of 10 mm, three waveband models (visible, short-NIR and visible-short-NIR regions) for unsaturated absorption were also established respectively. In independent validation, the models of all wavebands in the cases of 1 mm and 10 mm have achieved good discrimination effects. For the case of 1 mm, the visible model achieved the optimal validation effect, the validation recognition-accuracy rate (RARV) was 99.6%;while in the case of 10 mm, both the visible and visible-short-NIR models achieved the optimal validation effect (RARV = 100%). The detection method does not require reagents and is fast and simple, which is easy to promote the application. The results can provide valuable reference for designing small dedicated spectrometers with different measurement modals and different spectral regions.

Comparing simulated and experimental spectral line splitting in visible spectroscopy diagnostics in the HL-2A tokamak

We established the passive-visible spectroscopy diagnostics(P-VSD)and active-VSD(A-VSD)spectral splitting models for the HL-2A tokamak.Spectral splitting due to the influence of electromagnetic fields on the spectra in VSD is studied.Zeeman splitting induced by the magnetic field(B)is used to distinguish reflected light overlap in the divertor for P-VSD.Stark splitting caused by the Lorentz electric field(E_(Lorentz))from the neutral beam injection particle’s interaction with the magnetic field(V_(beam)×B)is used to measure the safety factor q profile for A-VSD.We give a comparison and error analysis by fitting the experimental spectra with the simulation results.The distinguishing of edge(scrape-off layer and divertor)hydrogen/deuterium spectral lines and the q profile derived from the spectra provides a reference for HL-2M VSD.

Infrared Spectroscopy-Based Chemometric Analysis for Lard Differentiation in Meat Samples

One of the most pressing concerns for the consumer market is the detection of adulteration in meat products due to their preciousness.The rapid and accurate identification mechanism for lard adulteration in meat products is highly necessary,for developing a mechanism trusted by consumers and that can be used to make a definitive diagnosis.Fourier Transform Infrared Spectroscopy(FTIR)is used in this work to identify lard adulteration in cow,lamb,and chicken samples.A simplified extraction method was implied to obtain the lipids from pure and adulterated meat.Adulterated samples were obtained by mixing lard with chicken,lamb,and beef with different concentrations(10%–50%v/v).Principal component analysis(PCA)and partial least square(PLS)were used to develop a calibration model at 800–3500 cm^(−1).Three-dimension PCA was successfully used by dividing the spectrum in three regions to classify lard meat adulteration in chicken,lamb,and beef samples.The corresponding FTIR peaks for the lard have been observed at 1159.6,1743.4,2853.1,and 2922.5 cm−1,which differentiate chicken,lamb,and beef samples.The wavenumbers offer the highest determination coefficient R2 value of 0.846 and lowest root mean square error of calibration(RMSEC)and root mean square error prediction(RMSEP)with an accuracy of 84.6%.Even the tiniest fat adulteration up to 10%can be reliably discovered using this methodology.

Process Characterization of the Transesterification of Rapeseed Oil to Biodiesel Using Design of Experiments and Infrared Spectroscopy

For optimization of production processes and product quality,often knowledge of the factors influencing the process outcome is compulsory.Thus,process analytical technology(PAT)that allows deeper insight into the process and results in a mathematical description of the process behavior as a simple function based on the most important process factors can help to achieve higher production efficiency and quality.The present study aims at characterizing a well-known industrial process,the transesterification reaction of rapeseed oil with methanol to produce fatty acid methyl esters(FAME)for usage as biodiesel in a continuous micro reactor set-up.To this end,a design of experiment approach is applied,where the effects of two process factors,the molar ratio and the total flow rate of the reactants,are investigated.The optimized process target response is the FAME mass fraction in the purified nonpolar phase of the product as a measure of reaction yield.The quantification is performed using attenuated total reflection infrared spectroscopy in combination with partial least squares regression.The data retrieved during the conduction of the DoE experimental plan were used for statistical analysis.A non-linear model indicating a synergistic interaction between the studied factors describes the reactor behavior with a high coefficient of determination(R^(2))of 0.9608.Thus,we applied a PAT approach to generate further insight into this established industrial process.

Matching Performance among Visible and near Infrared Coating,Low Infrared Emitting Coating and Microwave Absorbing Coating

The matching performance among the visible and near infrared coating.the low infrared emitting coating and the microwave absorbing coating was investigated.Experimental results show that the resulting malerial is characteristic of wideband effect ranging from the visible,near infrared and 3-5μm,8-14μm infrared protion of the spectrum,as well as the radar region from 8 to 18GHz when these three materials form αlayerstructure material system.The microwave absorbing ability of material is hardly changed.The resonance peak moves towards lower frequency as the thickness of the visible,near infrared coating and the low infrared emitting coating increases.This problem can be resolved by controlling the thickness of the matrial.On the other hand, the infrared emissivity εof the material system increases as the thickness of the visible,near infrared coating increases.This can be resolved by increasing infrared transparency of the visible and near infrared topcoating or controlling its thickness.The experimental resulting material system has spectral reflection characteristics in visible and near infrared regions that are similar to those of the natural background.

Intelligent Fusion of Infrared and Visible Image Data Based on Convolutional Sparse Representation and Improved Pulse-Coupled Neural Network

Multi-source information can be obtained through the fusion of infrared images and visible light images,which have the characteristics of complementary information.However,the existing acquisition methods of fusion images have disadvantages such as blurred edges,low contrast,and loss of details.Based on convolution sparse representation and improved pulse-coupled neural network this paper proposes an image fusion algorithm that decompose the source images into high-frequency and low-frequency subbands by non-subsampled Shearlet Transform(NSST).Furthermore,the low-frequency subbands were fused by convolutional sparse representation(CSR),and the high-frequency subbands were fused by an improved pulse coupled neural network(IPCNN)algorithm,which can effectively solve the problem of difficulty in setting parameters of the traditional PCNN algorithm,improving the performance of sparse representation with details injection.The result reveals that the proposed method in this paper has more advantages than the existing mainstream fusion algorithms in terms of visual effects and objective indicators.

An infrared and visible image fusion method based upon multi-scale and top-hat transforms

The high-frequency components in the traditional multi-scale transform method are approximately sparse, which can represent different information of the details. But in the low-frequency component, the coefficients around the zero value are very few, so we cannot sparsely represent low-frequency image information. The low-frequency component contains the main energy of the image and depicts the profile of the image. Direct fusion of the low-frequency component will not be conducive to obtain highly accurate fusion result. Therefore, this paper presents an infrared and visible image fusion method combining the multi-scale and top-hat transforms. On one hand, the new top-hat-transform can effectively extract the salient features of the low-frequency component. On the other hand, the multi-scale transform can extract highfrequency detailed information in multiple scales and from diverse directions. The combination of the two methods is conducive to the acquisition of more characteristics and more accurate fusion results. Among them, for the low-frequency component, a new type of top-hat transform is used to extract low-frequency features, and then different fusion rules are applied to fuse the low-frequency features and low-frequency background; for high-frequency components, the product of characteristics method is used to integrate the detailed information in high-frequency. Experimental results show that the proposed algorithm can obtain more detailed information and clearer infrared target fusion results than the traditional multiscale transform methods. Compared with the state-of-the-art fusion methods based on sparse representation, the proposed algorithm is simple and efficacious, and the time consumption is significantly reduced.

Fusion of Infrared and Visible Images Using Fuzzy Based Siamese Convolutional Network

Traditional techniques based on image fusion are arduous in integrating complementary or heterogeneous infrared(IR)/visible(VS)images.Dissimilarities in various kind of features in these images are vital to preserve in the single fused image.Hence,simultaneous preservation of both the aspects at the same time is a challenging task.However,most of the existing methods utilize the manual extraction of features;and manual complicated designing of fusion rules resulted in a blurry artifact in the fused image.Therefore,this study has proposed a hybrid algorithm for the integration of multi-features among two heterogeneous images.Firstly,fuzzification of two IR/VS images has been done by feeding it to the fuzzy sets to remove the uncertainty present in the background and object of interest of the image.Secondly,images have been learned by two parallel branches of the siamese convolutional neural network(CNN)to extract prominent features from the images as well as high-frequency information to produce focus maps containing source image information.Finally,the obtained focused maps which contained the detailed integrated information are directly mapped with the source image via pixelwise strategy to result in fused image.Different parameters have been used to evaluate the performance of the proposed image fusion by achieving 1.008 for mutual information(MI),0.841 for entropy(EG),0.655 for edge information(EI),0.652 for human perception(HP),and 0.980 for image structural similarity(ISS).Experimental results have shown that the proposed technique has attained the best qualitative and quantitative results using 78 publically available images in comparison to the existing discrete cosine transform(DCT),anisotropic diffusion&karhunen-loeve(ADKL),guided filter(GF),random walk(RW),principal component analysis(PCA),and convolutional neural network(CNN)methods.

Sub-Regional Infrared-Visible Image Fusion Using Multi-Scale Transformation

Infrared-visible image fusion plays an important role in multi-source data fusion,which has the advantage of integrating useful information from multi-source sensors.However,there are still challenges in target enhancement and visual improvement.To deal with these problems,a sub-regional infrared-visible image fusion method(SRF)is proposed.First,morphology and threshold segmentation is applied to extract targets interested in infrared images.Second,the infrared back-ground is reconstructed based on extracted targets and the visible image.Finally,target and back-ground regions are fused using a multi-scale transform.Experimental results are obtained using public data for comparison and evaluation,which demonstrate that the proposed SRF has poten-tial benefits over other methods.

Moving-window bis-correlation coefficients method for visible and near-infrared spectral discriminant analysis with applications

The moving window bis corelation coefficients(MW BiCC)was proposed and employed for the discriminant analysis of transgenic sugarcane leaves and B-thalassemia with visible and near-infrared(Vis NIR)spectroscopy.The well-performed moving window principal component analysis linear discriminant analysis(MWPCA-LDA)was also conducted for comparison.A total of 306 transgenic(positive)and 150 nont ransgenic(negative)leave samples of sugarcane were collected and divided to calibration,prediction,and validation.The diffuse reflection spectra were corected using Savitzky-Golay(SG)smoothing with first-order derivative(d=1),third-degree polynomial(p=3)and 25 smpothing points(m=25).The selected waveband was 736-1054nm with MW-BiCC,and the positive and negative validation recognition rates(V_REC^(+),VREC^(-))were 100%,98.0%,which achieved the same effect as MWPCA-LDA.Another example,the 93 B-thalassemia(positive)and 148 nonthalassemia(negative)of human hemolytic samples were colloctod.The transmission spectra were corrected using SG smoothing withd=1,p=3 and m=53.Using M W-BiCC,many best wavebands were selected(e.g.,1116-1146,17941848 and 22842342nm).The V_REC^(+)and V_REC^(-)were both 100%,which achieved the same effect as MW-PCA-LDA.Importantly,the BICC only required ca lculating correlation cofficients between the spectrum of prediction sample and the average spectra of two types of calibration samples.Thus,BiCC was very simple in algorithm,and expected to obtain more applications.The results first confirmed the feasibility of distinguishing B-thalassemia and normal control samples by NIR spectroscopy,and provided a promising simple tool for large population thalassemia screening.