Pulsed electrical stimulation protects neurons in the dorsal root and anterior horn of the spinal cord after peripheral nerve injury

Most studies on peripheral nerve injury have focused on repair at the site of injury, but very few have examined the effects of repair strategies on the more proximal neuronal cell bodies. In this study, an approximately 10-mm-long nerve segment from the ischial tuberosity in the rat was transected and its proximal and distal ends were inverted and sutured. The spinal cord was subjected to pulsed electrical stimulation at T10 and L3, at a current of 6.5 m A and a stimulation frequency of 15 Hz, 15 minutes per session, twice a day for 56 days. After pulsed electrical stimulation, the number of neurons in the dorsal root ganglion and anterior horn was increased in rats with sciatic nerve injury. The number of myelinated nerve fibers was increased in the sciatic nerve. The ultrastructure of neurons in the dorsal root ganglion and spinal cord was noticeably improved. Conduction velocity of the sciatic nerve was also increased. These results show that pulsed electrical stimulation protects sensory neurons in the dorsal root ganglia as well as motor neurons in the anterior horn of the spinal cord after peripheral nerve injury, and that it promotes the regeneration of peripheral nerve fibers.

Effects of Salt Stress on Root Vitality of Limonium bicolor (Bunge) Kuntze

[Objective] This study aimed to investigate the effects of different salt concentrations on the root vitality of Limonium bicolor （Bunge） Kuntze. [Method] Limonium bicolor （Bunge） Kuntze was treated with 0, 100, 200 and 400 mmol/L NaCl, respectively. After two weeks, root vitality, biomass and other physiological indicators were measured. [Result] Salt stress had significant influences on the growth of roots. Root vitality of Limonium bicolor increased firstly and reduced gradually with the increase of salt concentration. [Conclusion] The results indicate that Limonium bicolor has certain salt tolerance ability at low salt concentrations; under relatively high salt concentrations, Limonium bicolor roots can maintain high vitality; however, with the continuous increase of salt concentration, Limonium bicolor roots are damaged, with decreasing vitality.

Effects of Light Quality on Carbon and Nitrogen Assimilation and Root Activity of Eggplant

[Objective] This study was conducted to provide theoretical and data reference for high-quality high-yield production of Shangqie 1 under controlled light quality environment and light quality control of other eggplant varieties. [Method] With Shangqie1 as an experiment material, the effects of red light, blue light, red-blue light, red-blue light and white light(control) on the carbon-nitrogen metabolism and root vitality of eggplant were investigated. [Result] Different light quality significantly affected sugar and starch contents in leaves of eggplant. Under the red-blue light(5∶1) treatment, the sucrose, total sugar and starch contents were the highest, and the values were higher than those under the white light treatment by 3.47%, 13.61% and 33.49%,respectively. The fructose content was the highest under white light. Under red-blue light(3∶1) and red-blue light(5∶1), the nitrate nitrogen contents were higher than that under white light, indicating that compound light affects nitrate content not only by simple superposition of light quality, but also by other type of interaction effect. The free amino acid and soluble protein contents under blue, red-blue(3∶1), red-blue(5∶1) and red light treatments were all higher than those under the white light treatment, and the blue light treatment exhibited the highest values, which were higher than those under white light by 24.89% and 46.62%, respectively, with significant differences. The red-blue light(5 ∶1) treatment exhibited the highest root vitality of eggplant, which was higher than that under the white light treatment by 31.85%. The red light treatment had the second highest root vitality, which was higher than that under the white light treatment by 19.30%, followed by the red-blue light(5∶1) treatment, and the blue light treatment showed the lowest value. [Conclusion] Under red-blue light(5 ∶1) treatment, eggplant had vigorous carbon-nitrogen metabolism and the highest root vitality.

Optimization of back bead geometry in the PMAG-TIG twin arc hybrid root welding process using grey based Taguchi method

This study investigated multi-response optimization of the pulse metal active gas-tungsten inert gas( PMAG-TIG) twin arc hybrid root welding process for an optimal parametric combination to yield favorable back bead geometry of welded joints using grey relational analysis and Taguchi method.Eighteen experimental runs based on an orthogonal array following the Taguchi method were performed to derive objective functions to be optimized within the experimental domain.The objective functions were selected in relation to parameters of PMAG-TIG twin arc root welding back bead geometry: back bead width to root reinforcement ratio and deposited metal height.The Taguchi approach was followed by grey relational analysis to solve the multi-response optimization problem.The significance of factors on overall quality characteristics of the weld joint was also evaluated quantitatively using analysis of variance.Optimal results were verified through additional experiments,and showed to feasibility of applying grey relation analysis in combination with Taguchi technique for continuous improvement of product quality in the manufacturing industry.

Effect of Nutrient Media and KNO3 on <i>in Vitro</i>Plant Regeneration in <i>Saraca asoca</i>(Roxb.) Willd

Forest trees in general and those belonging to family Fabaceae in particular, have proved to be recalcitrant for propagation through tissue culture. Saraca asoca (Roxb.) Willd. (Family-Caesalpinaceae) is one such tree which has become vulnerable in nature due to over exploitation of its bark. Four nutrient media [MS (Murashige and Skoog Medium), WPM (Woody Plant Medium), B5 (Gamborg’s Medium) and NN (Nitsch and Nitsch Medium, 1969)] and five doses of BA (N6-Benzyladenine) (0, 2.2, 4.4, 8.8 and 17.8 μM) and their all possible interactions were tested for shoot induction and proliferation from nodal segments of 3-year-old plants. B5 medium supplemented with 2.2 μM BA was screened out as the most suitable medium shoot induction, proliferation and elongation of regenerated shoots. In order to enhance shoot number, the nitrogen source in B5 medium was modified and five strengths of KNO3 (0.25×, 0.5×, 1.0×, 1.25× and 1.5×) were tested. The different strengths of KNO3 (Potassium nitrate) had statistically significant effect on number of shoots and on 0.25× strength of KNO3, maximum number of shoots (1.92) were obtained. The modified strengths of KNO3 did not significantly affect the elongation of shoots. Effect of 5 durations (quick dip, transfer of shoots after 1 day, after 3 days, after 5 days and after 7 days) of pulse treatment with 200 μM IBA (Indole-3-butyric acid) in 1/2 strength MS liquid medium was tested. Thereafter, the shoots were transferred to semi-solid half strength MS medium supplemented with 0.2 μM IBA and 3.96 μM phloroglucinol. Pulse treatment of 5 days duration resulted in 37.5% in vitro rooting of shoots. Plantlets were hardened in soilrite soaked with half strength MS medium in culture room and later shifted to a soil mixture in shade house.

Horizontally root fractured teeth with pulpal vitality——two case reports

This case study reports the successful outcome of horizontal root fractures of two different patients, which took place in permanent incisors. Report 1 describes a case of a 29-year-old patient who suffered a mandibular trauma affecting mainly the lower central incisors, caused by a car accident. A panoramic radiograph was taken right after the accident and showed a horizontal root fracture in the middle third of tooth 42, which went untreated. Report 2 illustrates a case of a 17-year-old male patient who searched for orthodontic therapy and the periapical radiograph showed horizontal root fracture in tooth 11 caused by a previous trauma, which went untreated as well. There was healing through the reestablishment of pulp activity and dental coloration without professional intervention.

生物陶瓷材料iRoot BP Plus在年轻恒牙活髓保存治疗术中的临床价值分析

目的探讨生物陶瓷材料iRoot BP Plus在年轻恒牙活髓保存治疗术中的临床效果。方法将90例行活髓保存治疗术的患者按术中所用材料的不同分成iRoot组、 MTA组与氢氧化钙组各30例,术中予以覆盖相应的盖髓材料,评价各组的治疗效果及随访牙髓活力状态。结果 iRoot组、 MTA组的总有效率均高于氢氧化钙组(P <0.05),但iRoot组、 MTA的总有效率比较无统计学差异(P>0.05)。治疗后牙髓活力测试结果显示, iRoot组的正常率最高,其次为MTA组,氢氧化钙组最低, iRoot组与MTA组的正常率比较无统计学差异(P>0.05),而氢氧化钙组的正常率低于iRoot组与MTA组(P <0.05)。结论行牙髓活髓保存治疗术中应用生物陶瓷材料iRoot BP Plus具有显著效果,可改善患者的牙髓活力,值得推广。

Low-Intensity Pulsed Ultrasound Promote Schwann Cells Pro-Myelination Activities and Neurite Outgrowth of Dorsal Root Ganglion Neurons

In this study,we investigate how Schwann cells and dorsal root ganglion(DRG)neurons response to direct low-intensity pulsed ultrasound(LIPUS)stimuli in vitro.Primary Schwann cells and DRG were isolated from rat sciatic nerve and spine,respectively.LIPUS with varied dose of intensity(low:250 mW/cm2,medium:500 m W/cm2,high:750 m W/cm2)were applied 5 min per time for every other day,and pro-myelination indicators of Schwann cell as well as neurite outgrowth of dorsal root ganglion neurons were analyzed.Our results demonstrated that LIPUS promoted Schwann cells activity and proliferation from day 3 at the highest intensity,and day 5 at all intensities.In addition,LIPUS boosted pro-myelination activities of Schwann cells,as evidenced by increased cell population that positive for immunohistochemical staining against S100,nerve growth factor receptor(NGFR)p75,glial fibrillary acidic protein(GFAP),myelin protein zero(P0),as well as up-regulation of GFAP,Protein 0,nerve growth factor(NGF),and brain derived neurotrophic factor(BDNF)genes.Furthermore,LIPUS significantly enhanced the neurite outgrowth of DRG,with the highest intensity exhibiting longest neurite outgrowth.Taken together,our results strongly improve the understanding of cellular mechanisms of ultrasonic therapies for peripheral nerve repair.

不同生境下烤烟三段式育苗微生物群落变化及抗逆酶活分析

【目的】为解析三段式育苗对烟苗根际微生物多样性和抗逆酶活性的影响。【方法】以中烟100为供试品种,以常规漂浮育苗为对照,设置三段式育苗。通过监测工厂化四联体育苗大棚中烟苗的生长发育情况,系统分析了三段式育苗方式对育苗环境,育苗池、基质和根系微生物多样性,烟苗农艺性状和抗逆酶活性的影响。同时,追溯分析三段式育苗对于田间烟草根际土壤微生物多样性的影响。【结果】幼苗期,三段式育苗方式相对于漂浮育苗对照,改善了育苗池水体的理化性状,降低了育苗大棚内的温差和湿度水平,日均温差由19.23℃下降至17.95℃,日均湿度由75.17%RH下降至69.53%RH;成苗期,三段式育苗增加了育苗池水体中的短波单胞菌属(Brevundimonas)和赖氨酸芽孢杆菌属(Lysinibacillus)的丰度,分别为漂浮育苗的13.90倍和4.66倍。增加了烟苗的鲜重、根长、侧根数、CAT酶活性、SOD酶活性、POD酶活性、根活力和成苗率,较漂浮育苗对照分别提高了34.33%、34.37%、36.41%、31.91%、25.34%、58.07%、29.08%和10.91%,MDA含量降低了22.31%;大田期,提升了烟草根际土壤微生物子囊菌门和青梅属的丰度,创造了更有利的烟草生长环境。【结论】三段式育苗通过改善育苗环境,增加了烟苗根际有益菌群的丰度,显著提高了烟苗的成苗期农艺性状和抗逆酶活性,从而提升烟苗素质。

调亏-增氧灌溉对超级稻根系生长的影响

为提高农田灌溉用水利用效率,明确调亏-增氧灌溉对超级稻根系形态特征、生理特性、植株农艺性状及产量的影响,以超级稻中早39作为盆栽试验对象,设置灌溉增氧水0~30 mm、调亏灌溉土壤体积饱和含水率的百分比60%~80%和80%~100%3种调亏组合,测定分蘖中期、分蘖后期、拔节孕穗期的株高、有效穗数、植株干重、总根长、根表面积、根平均直径、总根尖数、根系活力和根系丙二醛(MDA)含量指标。结果表明:(1)调亏-增氧灌溉可提升超级稻根系活力,延缓分蘖后期、拔节孕穗期根系活力的减弱,有效降低超级稻根系MDA含量;(2)调亏-增氧灌溉有利于超级稻有效穗数的增加和植株干重的积累;(3)调亏-增氧灌溉对超级稻根表面积、总根长、根平均直径、总根尖数有明显提高。总之,调亏-增氧灌溉对超级稻根系生长影响显著,促进水资源利用效率,促进水稻产量形成。

四川南充碱性紫色土春见桔橙园不同水平施磷的效果

为探寻四川省南充市碱性紫色土春见桔橙绿色优质生产的适宜施磷量,以7年生香橙砧春见桔橙为试材,在年施用相同氮肥(尿素,以N计,350 g·株^(-1)·年^(-1))和钾肥(硫酸钾,以K_(2)O计,为347 g·株^(-1)·年^(-1))的条件下,设置不同施磷水平(过磷酸钙,以P_(2)O_(5)计,61、92、125、154、180、215 g·株^(-1)·年^(-1))处理,比较不同处理春见桔橙根系活力、土壤酶活及果实内在品质,并采用主成分分析法对不同处理的综合效果进行评价。结果表明,随着施磷水平提高,根系活力先增加后降低,土壤有效磷含量先上升后下降,碱性磷酸酶与蔗糖酶活性均先增后降,土壤脲酶活性变化没有规律性,施磷水平对土壤过氧化氢酶活性的影响不显著,果实可溶性固形物含量总体下降,可滴定酸含量先降低后升高,固酸比总体上升,维生素C含量先降低后升高,以180 g/株处理的综合效果最好。

基层党建思想政治工作的重要性与运用

党的建设是一个永恒的课题,尤其是在新时代下,党的建设更是成为一项极其重要的任务。而基层党建则是整个党建工作的重中之重,更是各级党组织必须高度重视的工作。基层党建工作旨在建设具有强大组织力和战斗力的党组织,使得党的思想政治路线能够贯彻落实到每一个基层单位。因此,文章围绕基层党建思想政治工作的重要性以及开展该项工作的举措等方面展开探讨。

银质针疗法联合颈2背根神经节脉冲射频治疗颈源性头痛的疗效分析

目的 分析银质针疗法联合颈2背根神经节脉冲射频治疗颈源性头痛的效果。方法 选出80例颈源性头痛患者作为研究对象,按照治疗方法不同分成对照组和观察组,每组40例。对照组采用颈2背根神经节脉冲射频治疗,观察组采用银质针疗法联合颈2背根神经节脉冲射频治疗。对比两组临床治疗效果,治疗前后的视觉模拟评分法(VAS)评分、颈椎关节活动度(ROM)、颈部血管搏动指数(PI)、阻力指数(RI)、椎动脉平均血流速度(Vm),治疗满意度。结果 观察组治疗总有效率92.50%高于对照组的75.00%,对比有差异(P<0.05)。治疗后,两组VAS评分低于本组治疗前, ROM大于本组治疗前,且观察组VAS评分(2.05±0.34)分低于对照组的(5.22±1.06)分, ROM(41.04±8.21)°大于对照组的(34.18±8.57)°,对比有差异(P<0.05)。治疗后,两组PI、RI小于本组治疗前, Vm大于本组治疗前,且观察组PI(0.61±0.42)、RI(0.51±0.07)小于对照组的(0.85±0.53)、(0.61±0.11), Vm(36.98±6.38)cm/s大于对照组的(30.13±5.92)cm/s,对比有差异(P<0.05)。观察组满意度97.50%高于对照组的85.00%,对比有差异(P<0.05)。结论 联合治疗(银质针疗法+颈2背根神经节脉冲射频)可有效缓解颈源性头痛患者的疼痛和活动受限,提高临床治疗效果,改善血流动力学,值得临床推广。

外源钙对丝瓜植株形态建成、光合色素、光合能力及根系活力的影响

为探究外源钙对丝瓜生长与生理特性的影响机理,探寻适宜丝瓜生长的外源钙浓度,本试验借助无土栽培的方式,设置不同外源钙浓度,研究其对丝瓜植株形态建成、光合色素、光合能力及根系活力的影响。结果表明,适宜浓度的外源钙对丝瓜株高、茎粗、叶面积和根长有正向促进作用,且外源钙浓度在12 mmol/L时最佳,较对照组CK分别增加19.47%、25.15%、19.60%和17.85%,差异均具有统计学意义(P<0.05)。叶片叶绿素含量随着外源钙浓度增加表现出先升高后降低的趋势。外源钙对叶片净光合速率和根系活力的影响均表现出低浓度促进、高浓度抑制的调节特点,且以外源钙浓度12 mmol/L时值相对较大,较对照组分别提高11.22%、19.01%。综合来看,12mmol/L的外源钙浓度有利于丝瓜植株的形态建成、光合能力的增强和根系活力的提升。

杨文信教授中西医结合治疗颜面再发性皮炎经验

颜面再发性皮炎是临床上常见的颜面部红斑鳞屑性疾病,多见于中青年女性,主要临床表现为颜面部轻度局限性红斑、细小糠状鳞屑,自觉瘙痒,易反复发生。该病发病原因不明,不但影响颜面部美观,还对患者造成严重身心损害,甚至使患者产生抑郁、焦虑等心理障碍,因此为皮肤科医师所重视。导师杨文信教授为四川省名中医,从事中医药临床和科研工作30余年,经过长期的临床实践和对外交流,积累了丰富的临床经验,尤其是对皮肤性病的认识和辨证治疗有较深的造诣。杨文信教授认为,颜面再发性皮炎主要是因为外感湿热毒邪,血热之邪上行,搏结于面部而发病,主张以血热为本,从湿、热、毒方面,结合舌、脉、症之辨证统一论治,治法以清热凉血疏风为主,方选九味消风散化裁,采用血热为本、脉症合参为主的中西医结合疗法进行治疗,同时注重对患者的心理疏导及良好的生活饮食习惯培养,以提高患者的治疗依从性。笔者有幸师从杨文信教授,颇有收获,现介绍杨文信教授结合西南地区的地域特点治疗颜面再发性皮炎的经验,为临床治疗该病提供参考。

观察超声引导下颈神经根脉冲射频配合小针刀治疗神经根型颈椎病的临床效果及安全性

目的探讨超声(US)引导下颈神经根脉冲射频(PRF)配合小针刀治疗在神经根型颈椎病(CSR)中的应用效果.方法选取我院2021年6月—2023年6月收治的90例CSR患者为研究对象,按随机数字表法将其分为对照组和观察组,每组45例.对照组采用小针刀治疗,观察组在对照组基础上加用US引导下颈神经根PRF治疗.比较两组的临床效果及安全性.结果观察组治疗总有效率为95.96%,高于对照组的82.22%,差异有统计学意义(P﹤0.05).治疗后,观察组疼痛视觉模拟评分、颈椎功能障碍指数评分均低于对照组,CASCS评分高于对照组,组间差异有统计学意义(P﹤0.05);观察组颈椎曲度、屈伸活动度均大于对照组,组间差异有统计学意义(P﹤0.05);观察组生活质量各领域评分均高于对照组,组间差异有统计学意义(P﹤0.05);两组均无并发症.结论US引导下颈神经根PRF配合小针刀治疗可提高CSR的疗效,降低VAS评分,能减轻颈椎功能障碍,改善患者生活质量.

水稻根际含氧量对根系生长的影响

以多个水稻品种为材料,采用营养液培养试验,通过气泵增加水稻根际的含氧量,研究水稻根际含氧量与根系生长的关系。结果表明,在水稻生长过程中,抽穗期根系吸收氧气的能力最强,需氧量最大。根际含氧量对水稻根系形态与分布有影响,增氧显著增加根长,提高根系自根基到根尖10-20cm、〉20cm部分所占的生物量比例,同时减少根数;而在缺氧环境下生长的水稻根数增多,根长缩短。氧溶量对水稻根系活力的影响存在品种间差异,提高水稻根际的氧溶量,杂交稻汕优63和国稻1号齐穗期根系的活跃吸收面积和根系活力显著增加;根系活力较高的常规籼稻湘早籼11和甬粳18在充氧处理中的根系活力也明显提高,但根际氧含量的增加对根系活力较低的水稻品种湘早籼24和春江06的根系活力并无明显作用;缺氧环境下生长的水稻根系的活力降低。

高温胁迫对仙客来根系活力和叶片生化指标的影响

仙客来的两个品种在 35℃高温胁迫下 6 0h后根系活力急剧下降 ,同时叶片细胞膜脂过氧化产物丙二醛开始升高。伴随着根吸收能力下降 ,叶片含水量的降低 ,游离脯氨酸积累急剧上升。超氧物歧化酶 (SOD)活性表现出先升高 ,后迅速下降的趋势。这些变化同仙客来遇高温后停止生长。

水稻根系活力的遗传分析

以典型籼粳交 (窄叶青 8号 /京系 17) F1 代花培加倍的 DH群体为材料 ,在抽穗期用 TTC法考察根系活力。利用已构建的分子连锁图谱 ,采用区间作图法对根系活力进行 QTL 分析 ,在第 4染色体的 RG4 4 9和 RG80 9之间检测到一个QTL。用 Epistat软件分析影响根系活力的单个位点和位点间的互作 ,结果在 RG80 9附近检测到一个单基因 ,并检测到 2个条件型互作和 4对互适型互作 ,这些位点分布在第 1、2、4、7、8、9和

铺地木蓝对不同程度淹水胁迫的生理响应

应用于感潮河涌两岸或具有季节性消涨带河道景观绿化的植物必须能耐水淹。本试验以铺地木蓝为对象,研究了不同淹水深度和淹水时间下,铺地木蓝的存活率、叶绿素含量、根系活力和根冠比等指标的变化情况,旨在探索铺地木蓝对淹水生境的响应机制,阐明铺地木蓝耐淹的生理机理,为该植物种用于感潮河涌或有水位变化的河道两岸绿化工程的可行性提供理论依据。结果表明,1)经过21d淹水胁迫,铺地木蓝的存活率达到100%,虽然其叶绿素含量显著下降,但能够长时间维持在一个比较稳定的水平;2)随着淹水时间的延长,地下根的根系活力显著下降,全淹处理下淹水茎部无不定根形成,而半淹处理下淹水茎部不定根迅速形成且根系活力较强;3)淹水后铺地木蓝的茎叶生物量大幅下降,根冠比增加。