Multifactors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition.However, few reports have investigated the relative capacity of different factors to shape the gut microbiome ...Multifactors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition.However, few reports have investigated the relative capacity of different factors to shape the gut microbiome in a single study. Our design used a genetic vitamin A-deficient mouse model, the Rbp4^(-/-)mouse,feeding with the low vitamin A diets at different ages of initiation(4 or 7 weeks) for 28 days. Fecal samples were collected for bacterial profiling at seven time points after diet controlling. With Rbp4 depletion,Akkermansia decreased and Bacteroides increased, whereas Desulfovibrio, Barnesiella, Clostridium_Xl Va,and Lactobacillus fluctuated. The bacterial community swiftly adjusted with the vitamin A-deficient diet administration and gradually changed(e.g., decrease of Barnesiella and increase of Desulfovibrio). Age exerted a relatively weaker but long-last influence. At an earlier age to feed a vitamin A-deficient diet, a higher microbial dysbiosis index will be valued. Of note, the shaping effects of diet and age on the bacterial community varied with the difference of genotype, which might indicate a greater role of genotype than diet and age in shaping the gut microbiome.展开更多
基金funded by the KCL and PKUHSC Joint Institute for Medical Research Fund(BMU2020KCL003)the National Natural Science Foundation of China(No.81900984,81991501,81870747,82000496,82070566,and 81870387)+2 种基金the Beijing Municipal Natural Science Foundation(No.7182184 and 7214267)Capital’s Funds for Health Improvement and Research(2020-2Z-40813)Peking University Clinical Medicine Plus X-Young Scholars Project(PKU2021LCXQ003)。
文摘Multifactors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition.However, few reports have investigated the relative capacity of different factors to shape the gut microbiome in a single study. Our design used a genetic vitamin A-deficient mouse model, the Rbp4^(-/-)mouse,feeding with the low vitamin A diets at different ages of initiation(4 or 7 weeks) for 28 days. Fecal samples were collected for bacterial profiling at seven time points after diet controlling. With Rbp4 depletion,Akkermansia decreased and Bacteroides increased, whereas Desulfovibrio, Barnesiella, Clostridium_Xl Va,and Lactobacillus fluctuated. The bacterial community swiftly adjusted with the vitamin A-deficient diet administration and gradually changed(e.g., decrease of Barnesiella and increase of Desulfovibrio). Age exerted a relatively weaker but long-last influence. At an earlier age to feed a vitamin A-deficient diet, a higher microbial dysbiosis index will be valued. Of note, the shaping effects of diet and age on the bacterial community varied with the difference of genotype, which might indicate a greater role of genotype than diet and age in shaping the gut microbiome.
