科宝500和AA白羽肉鸡生产性能比较分析

文章旨在探讨科宝500和爱拔益加(AA)白羽肉鸡生产性能的差异。试验以科宝500和爱拔益加(AA)白羽肉鸡为研究对象,测定1~6周龄两个品种的平均采食量、平均体重、平均日增重、料重比、死淘率以及欧洲效益指数,分析比较两品种间生产性能的差异变化。结果表明:(1)科宝500肉鸡平均采食量前期变化较大,2周龄时显著高于AA肉鸡(P<0.05),但3周龄显著低于AA白羽肉鸡(P<0.05),整个试验期(1~6周龄),科宝500肉鸡平均采食量高于AA肉鸡,但差异不显著(P>0.05);(2)7和35日龄时,科宝500肉鸡平均体重显著大于AA肉鸡(P<0.05),5周龄时,科宝500肉鸡平均日增重显著高于AA肉鸡(P<0.05),而其他周龄及整个试验期(1~6周龄),科宝500肉鸡平均体重和平均日增重均高于AA肉鸡,但差异均不显著(P>0.05);(3)各周龄及整个试验期(1~6周龄)科宝500肉鸡料重比均低于AA肉鸡,但差异均不显著(P>0.05);(4)4周龄时,科宝500肉鸡死淘率显著高于AA肉鸡(P<0.05),而其他周龄及整个试验期(1~6周龄),科宝500肉鸡死淘率高于AA肉鸡,但差异均不显著(P>0.05);(5)欧洲效益指数(EPI)方面,科宝500肉鸡高于AA肉鸡,但差异不显著(P>0.05)。综合分析,科宝500肉鸡生产性能及养殖效益优于AA肉鸡。

土壤游离铁CS AAS测定方法的验证

为提高游离铁的检测效率,本研究对游离铁(Fed)的测定(DCB法)提取液在原子吸收光谱仪上的检测进行验证。使用CS AAS上机时对特征参数包括标准曲线、检出限、检出下限、精密度、准确度进行验证及确认。确定线性范围为0~7.0 mg/L,非线性拟合回归方程相关系数0.999 8、检出限0.005 3 g/kg、检出下限0.021 g/kg。普通土壤样品检测精密度相对相差小于10%,相对标准偏差小于1.9%,满足方法中平行测定质量控制要求和《化学分析方法验证确认和内部质量控制要求》。对高、中、低3个有证标准物质进行6次测定,相对误差小于10%,满足正确度的要求,上机测试数据稳定可靠。

汉日语言AA式名词叠词与数字的搭配考察

汉日语言中都存在大量AA式名词叠词,但有所不同的是像“年年”这样的同形同义词在汉语中可以和数字“一”共起,在日语中却不行。文章通过词典收集语料后,再将语料逐一输入语料库对汉日AA式名词叠词与数字的搭配见考察,发现汉语AA式名词叠词有“只能与数字‘一’搭配”和“可以和所有数字搭配”两种类型,日语AA式与数字搭配的名词叠词有“不能与数字‘一’搭配”和“可以和所有数字搭配”两种类型。并且汉语AA式名词叠词与数字搭配情况更为普遍,搭配上也更具灵活性。

Participant attrition and perinatal outcomes in prenatal vitamin Dsupplemented gestational diabetes mellitus patients in Asia: A metaanalysis

BACKGROUND The role of vitamin D supplementation in gestational diabetes mellitus(GDM)patients is unclear.AIM To determine the burden and risk of post-randomization GDM patient attrition from vitamin D-supplemented arms of randomized controlled trials(RCTs).The auxiliary aim was to compare the effects of nutritional supplements on their fasting blood glucose(FPG)levels and perinatal outcomes.METHODS RCTs were searched in the PubMed,Embase,and Scopus databases.Randomeffect prevalence and pairwise meta-analysis were performed for the primary objective.The auxiliary aim was to compare the effects of nutritional supplements on their fasting blood glucose(FPG)levels and perinatal outcomes.Fixed-effect network meta-analyses were undertaken for the secondary goals.All analyses were performed using Stata software,and statistical significance was determined at P<0.05.RESULTS Thirteen RCTs from Iran and China were reviewed.The participant attrition burden in vitamin D recipients was 6%[95%confidence interval(CI):0.03,0.10],and its risk did not vary from non-recipients.Vitamin D and calcium co-supplementation reduced the cesarean section incidence in GDM patients[risk ratio(RR):0.37;95%CI:0.18,0.74].The hyperbilirubinemia or hospitalization risk in their newborns decreased with vitamin D supplementation(RR:0.47;95%CI:0.27,0.83)and co-supplementation with calcium(RR:0.35;95%CI:0.16,0.77)or omega3 fatty acids(RR:0.25;95%CI:0.08,0.77).Vitamin D and probiotics co-supplementation decreased newborn hyperbilirubinemia risk(RR:0.28;95%CI:0.09,0.91).FPG levels and macrosomia risk did not vary across interventions.CONCLUSION In RCTs,vitamin D supplementation or co-supplementation in GDM patients showed a low participant attrition burden and low risk of cesarean section,newborn hyperbilirubinemia,and newborn hospitalization.

circMYO9A_006通过翻译蛋白MYO9A-208aa发挥抑制心肌细胞肥大的作用

目的:研究环状RNA MYO9A_006(circMYO9A_006)对心肌细胞肥大表型的调控作用及可能机制。方法:利用腺病毒介导在乳小鼠心室肌细胞(NMVCs)中过表达circMYO9A_006,并以腺病毒介导过表达同样带有绿色荧光蛋白标签的空载体为对照,检测NMVCs中心肌肥大相关蛋白β-肌球蛋白重链(β-MHC)、骨骼肌肌动蛋白α1(ACTA1)和心房钠尿肽(ANP)的表达。建立去氧肾上腺素(PE)诱导的乳大鼠心室肌细胞(NRVCs)肥大模型,通过鬼笔环肽染色检测过表达circMYO9A_006对NRVCs表面积的影响。双萤光素酶报告基因实验检测circMYO9A_006包含的潜在内部核糖体进入位点(IRES)的活性。通过Western blot实验检测circMYO9A_006翻译蛋白MYO9A-208aa及其在细胞内分布情况。分别制备circMYO9A_006-ORF(直接表达MYO9A-208aa)和circMYO9A_006-ATG-mut(不能表达MYO9A-208aa)重组病毒,以空载体病毒和circMYO9A_006重组病毒分别感染NRVCs,检测circMYO9A_006翻译蛋白MYO9A-208aa对心肌细胞肥大表型的特异调节作用。结果:利用腺病毒可在NMVCs中有效介导circMYO9A_006过表达。过表达circMYO9A_006可显著抑制NMVCs中心肌肥大相关蛋白的表达(P<0.01),并显著抑制PE诱导的NRVCs中心肌肥大相关蛋白的表达和细胞表面积的增加(P<0.05)。双萤光素酶报告基因实验结果提示,circMYO9A_006包含的2个IRES均具有活性。Western blot检测结果显示,在NRVCs中过表达circMYO9A_006可翻译预期大小为28 kD的MYO9A-208aa蛋白,并主要分布于细胞质中。过表达MYO9A-208aa和circMYO9A_006可一致地抑制NRVCs心肌肥大相关蛋白表达(P<0.01),并可逆转PE诱导的NRVCs肥大反应(P<0.05);而过表达circMYO9A_006-ATG-mut没有抑制NRVCs肥大的作用。结论:circMYO9A_006通过翻译蛋白MYO9A-208aa发挥抑制心肌细胞肥大的作用。

Vitamin D,selenium,and antidiabetic drugs in the treatment of type 2 diabetes mellitus with Hashimoto's thyroiditis

BACKGROUND Diabetes and thyroiditis are closely related.They occur in combination and cause significant damage to the body.There is no clear treatment for type-2 diabetes mellitus(T2DM)with Hashimoto's thyroiditis(HT).While single symptomatic drug treatment of the two diseases is less effective,combined drug treatment may improve efficacy.AIM To investigate the effect of a combination of vitamin D,selenium,and hypoglycemic agents in T2DM with HT.METHODS This retrospective study included 150 patients with T2DM and HT treated at The Central Hospital of Shaoyang from March 2020 to February 2023.Fifty patients were assigned to the control group,test group A,and test group B according to different treatment methods.The control group received low-iodine diet guidance and hypoglycemic drug treatment.Test group A received the control treatment plus vitamin D treatment.Test group B received the group A treatment plus selenium.Blood levels of markers of thyroid function[free T3(FT3),thyroid stimulating hormone(TSH),free T4(FT4)],autoantibodies[thyroid peroxidase antibody(TPOAB)and thyroid globulin antibody(TGAB)],blood lipid index[low-density lipoprotein cholesterol(LDL-C),total cholesterol(TC),triacylglycerol(TG)],blood glucose index[fasting blood glucose(FBG),and hemoglobin A1c(HbA1c)]were measured pre-treatment and 3 and 6 months after treatment.The relationships between serum 25-hydroxyvitamin D3[25(OH)D3]level and each of these indices were analyzed.RESULTS The levels of 25(OH)D3,FT3,FT4,and LDL-C increased in the order of the control group,test group A,and test group B(all P<0.05).The TPOAB,TGAB,TC,TG,FBG,HbA1c,and TSH levels increased in the order of test groups B,A,and the control group(all P<0.05).All the above indices were compared after 3 and 6 months of treatment.Pre-treatment,there was no divergence in serum 25(OH)D3 level,thyroid function-related indexes,autoantibodies level,blood glucose,and blood lipid index between the control group,test groups A and B(all P>0.05).The 25(OH)D3 levels in test groups A and B were negatively correlated with FT4 and TGAB(all P<0.05).CONCLUSION The combination drug treatment for T2DM with HT significantly improved thyroid function,autoantibody,and blood glucose and lipid levels.

构式语法框架下清涧方言“圪AA的”状态形容词研究

陕北晋语清涧方言中“圪AA的”状态形容词有180多个且在日常生活中使用频繁,按照构式语法属于四字格构式。形态上,该构式中A可以是形容词、动词和拟声词。“圪AA的”表达式一部分是通过其对应的“A”式“圪A”式、“AA的”式经重叠、加缀等形态变化生成,一部分是通过给“圪AA的”构式填充A后再将其重叠的结果。句法上,该构式可以在句中作谓语、定语、补语和状语,不受单音节否定词修饰,但可以用在“不/没说”含有对比的否定句中。该构式的基本义为描绘性,其语义和语用内涵包括“极致性”语义量值、“消极/负面性”立场态度偏向和“口语性”语体色彩。

Unlocking the potential-vitamin D in prostate cancer prevention

Prostate cancer poses a significant health challenge globally,demanding proactive prevention strategies.This editorial explores the emerging role of vitamin D in prostate cancer prevention.While traditionally associated with bone health,vitamin D is increasingly recognized for its broader impact on immune function,cellular signaling,and cancer prevention.Epidemiological studies suggest an intriguing link between vitamin D deficiency and elevated prostate cancer risk,particularly in regions with limited sunlight exposure.Mechanistically,vitamin D regulates cellular processes,inhibiting unchecked cancer cell growth and bols-tering immune surveillance.Personalized prevention strategies,considering individual factors,are deemed essential for harnessing the full potential of vitamin D.To unlock this potential,the future calls for robust research,public awareness campaigns,dietary improvements,and vigilant medical guidance.Collaborative efforts are poised to pave the way toward a future where vitamin D stands as a sentinel in prostate cancer prevention,ushering in hope and improved health for men worldwide.

日粮中添加琥珀酸对AA肉鸡生产性能和肠道健康的影响

试验旨在探究日粮中添加琥珀酸对AA肉鸡生产性能和肠道健康的影响。选用1日龄健康AA肉鸡240羽,根据体重随机分为4组,每组5个重复,每个重复12羽。对照组饲喂基础日粮,抗生素组在基础日粮中添加15 mg/kg维吉尼亚霉素,低剂量和高剂量琥珀酸组分别在基础日粮中添加1 g/kg和2 g/kg琥珀酸。试验期为42 d(1~21 d和22~42 d)。结果表明:与对照组相比,高剂量琥珀酸能够提高肉鸡全净膛率(P<0.05);与抗生素组相比,低剂量琥珀酸能够提高肉鸡血清尿酸和尿素氮水平(P<0.05);日粮中添加琥珀酸对肉鸡肠道形态结构和盲肠微生物组成影响较小,但与对照组相比,添加低剂量和高剂量琥珀酸具有下调肠道炎症因子基因IL-4 mRNA表达的趋势(P=0.0992和P=0.0728),添加高剂量琥珀酸还上调了IFN-γ mRNA的表达(P<0.05),提示日粮中添加琥珀酸有利于缓解肉鸡肠道炎症,调节免疫力。由此可见,日粮中添加高剂量(2 g/kg)琥珀酸能够促进肉鸡生长发育、缓解肠道炎症、调节免疫力,而添加低剂量(1 g/kg)琥珀酸则可以改善机体氨氮代谢水平。

AA7075-T6铝合金电阻点焊工艺参数优化研究

轻量化结构设计在保证了结构强度的前提下降低了整车质量,减少了能量消耗,成为汽车工业和航空航天的研究重点。本工作通过建立正交实验获取AA7075-T6铝合金的最佳焊接工艺参数。对接头进行拉伸剪切实验、金相观察和显微硬度测试,并对最优参数组合进行数值模拟,以研究焊接工艺参数对接头质量的影响。结果表明:当焊接时间为60 ms、焊接电流为17 kA、电极压力为0.22 MPa时,点焊接头的综合力学性能最好;焊接参数对电阻点焊接头力学性能的影响顺序为焊接时间>焊接电流>电极压力。焊接过程数值模拟得到的熔核尺寸与实验结果一致。焊接过程发生飞溅和熔核存在缩孔缺陷,导致接头的力学性能较差,失效模式为界面断裂。由于焊接过程接头显微组织发生变化,接头显微硬度变化呈“W”形。

Role of vitamin D in COVID-19 and other viral infections

Vitamin D is a steroid hormone that is naturally produced in the body or obtained through dietary sources,primarily under the influence of UVB radiation.This essential nutrient has a vital role in numerous physiological processes,encom-passing immune function,cell growth,differentiation,insulin regulation,and cardiovascular well-being,along with its pivotal role in sustaining the delicate equilibrium of calcium and phosphate concentrations in the body.Moreover,vitamin D reinforces mucosal defense and bolsters the immune system through immunomodulation,making it a critical component of overall health.Numerous studies have unveiled the profound connection between vitamin D and the predisposition to respiratory tract infections,including well-known viruses such as influenza and the novel severe acute respiratory syndrome coronavirus 2.Vita-min D deficiency has been consistently linked to increased severity of coronavirus disease 2019(COVID-19)and a heightened risk of mortality among afflicted individuals.Retrospective observational studies have further substantiated these findings,indicating that levels of vitamin D are linked with both the occurrence and severity of COVID-19 cases.Vitamin D has its influence on viral infections th-rough a multitude of mechanisms,such as promoting the release of antimicrobial peptides and fine-tuning the responses of the immune system.Additionally,vitamin D is intertwined with the intricate network of the renin–angiotensin system,suggesting a potential impact on the development of complications related to COVID-19.While further clinical trials and extensive research are warranted,the existing body of evidence strongly hints at the possible use of vitamin D as a valuable tool in the prophylaxis and management of COVID-19 and other viral infectious diseases.

玉米和小麦在AA肉鸡和黄羽肉鸡中的能值及养分代谢率评定

试验旨在比较玉米和小麦在AA肉鸡和黄羽肉鸡中的代谢能值及营养物质的表观代谢率,为玉米和小麦饲粮在家禽养殖生产上的应用提供理论基础。试验通过选择同日龄和相近体重的AA肉鸡和黄羽肉鸡分别进行两个代谢试验。同日龄试验:AA肉鸡和黄羽肉鸡在18日龄时分别各选择96羽,共192羽,AA肉鸡和黄羽肉鸡均随机分为玉米日粮和小麦日粮2个处理组,每个处理组8个重复,每个重复6羽。同体重试验;在试验鸡体重均达到600 g左右时分别在AA肉鸡和黄羽肉鸡中各选择96羽,试验设计分组与同日龄试验一致。试验结果表明,玉米在AA肉鸡中的表观代谢能(AME)、氮校正表观代谢能(AMEn)、粗蛋白和粗脂肪的表观代谢率显著高于同体重的黄羽肉鸡(P<0.05),与同日龄黄羽肉鸡相比没有显著差异;小麦在AA肉鸡中的AME、AMEn、粗蛋白和粗脂肪的表观消化率与同日龄和同体重的黄羽肉鸡相比均有提高,其中AME差异显著(P<0.05);AA肉鸡和黄羽肉鸡对玉米和小麦中淀粉的表观消化率没有显著差异(P>0.05)。综上所述,玉米在AA肉鸡中的能值和养分代谢率高于同体重黄羽肉鸡,小麦在AA肉鸡中的能值和养分表观代谢率高于同日龄和同体重的黄羽肉鸡。

基于AA-UNet的肝硬化辅助诊断系统

为准确地提取高频肝脏超声图像中的肝包膜,本文采用注意力门和空洞空间金字塔池化UNet(Attention gates and Atrous spatial pyramidal pooling UNet,AA-UNet)分割算法,对高频肝脏超声图像中的肝包膜进行识别,并根据肝包膜的物理形状判断肝硬化程度。首先,将数据增强后的高频肝脏超声图像送入网络模型进行训练;其次,将训练好的模型部署到肝硬化辅助诊断系统。实验结果表明,肝硬化辅助诊断系统能有效识别肝包膜区域并判断肝硬化程度。

AA2024铝合金板料敲击式渐进成形性能研究

为了探究合适的数控渐进成形工艺参数范围,确定更优的敲击式渐进成形性能方案,通过单因素试验法研究振幅、频率、工具头直径和层进给量4个因素对AA2024铝合金板料成形性能的影响规律。试验结果表明:与传统的增量成形方式相比,敲击式渐进成形方式会降低板料的成形极限角,板料的成形性能会有所降低;当振幅为5 mm时,成形极限角随着频率的增大而增大;当振幅为10 mm时,成形极限角随着频率的增大而减小,成形随层进给量增大而增大,随层工具头直径增大先减小后增大。

Ywhab通过靶向Hsp90aa1抑制小鼠B细胞淋巴瘤38B9细胞生长

目的:研究Ywhab在小鼠B细胞淋巴瘤发生发展中的作用,并探讨其可能的分子机制。方法:使用生物信息学分析Ywhab与弥漫性大B细胞淋巴瘤(DLBCL)的相关性。在小鼠B细胞淋巴瘤38B9细胞中感染逆转录病毒构建Ywhab基因过表达细胞系,并采用RT-qPCR和Western blot验证Ywhab的mRNA及蛋白(14-3-3β)水平;通过细胞计数法、CCK-8法和小鼠皮下成瘤实验检测Ywhab过表达对38B9细胞在体内外生长的影响;RT-qPCR和Western blot检测细胞凋亡相关蛋白的表达;应用蛋白质免疫共沉淀联合蛋白质谱(CoIP-MS)筛选与14-3-3β特异性结合的蛋白并采用Western blot及分子对接分析进行验证。结果:Ywhab基因在DLBCL中低表达,其低表达预示DLBCL患者的临床预后较差。与正常小鼠骨髓B细胞相比,Ywhab在38B9细胞中低表达。Ywhab过表达能在体内外诱导38B9细胞凋亡,促进凋亡蛋白Puma、Noxa和Bax的mRNA及蛋白表达,抑制抗凋亡蛋白Bcl2的mRNA及蛋白表达(P<0.05)。14-3-3β蛋白能特异性结合Hsp90aa1蛋白并抑制其表达,从而促进38B9细胞凋亡。结论:Ywhab能够显著诱导小鼠B细胞淋巴瘤38B9细胞凋亡,其机制可能是通过靶向抑制Hsp90aa1蛋白表达来实现的。

Coactivator-independent vitamin D receptor signaling causes severe rickets in mice,that is not prevented by a diet high in calcium,phosphate,and lactose

The vitamin D receptor(VDR)plays a critical role in the regulation of mineral and bone homeostasis.Upon binding of 1α,25-dihydroxyvitamin D_(3) to the VDR,the activation function 2(AF2)domain repositions and recruits coactivators for the assembly of the transcriptional machinery required for gene transcription.

Selenium,Type-2 Diabetes,and the Possible Protective Role of Vitamin D

Diabetes is a pervasive and serious global health issue.According to the International Diabetes Federation report,463 million adults worldwide were living with diabetes in 2019,and this number is projected to reach 700 million in 2045^([1]).

Current Insights on Vitamin D Intake, Status and Symptoms of Irritable Bowel Syndrome: A Narrative Review

The association between Vitamin D and IBS has attracted growing interest because of vitamin D’s involvement in immune system function and reduction of inflammation. IBS, a prevalent gastrointestinal condition marked by symptoms such as stomach pain, bloating, and changed bowel habits, implies that sufficient levels of Vitamin D may impact the symptomatology and quality of life associated with IBS. This review aimed to unveil current insights on vitamin D intake, status and symptoms of irritable bowel syndrome. A search was conducted on Embase, Scopus, Medline, and Cochrane Library, as well as a reference list of relevant studies. This paper also examined the current therapeutic practices for using vitamin D in the management of IBS. Although several studies demonstrate a positive association between vitamin D administration and the alleviation of IBS symptoms, the data is not yet definitive. Moreover, the review emphasised the necessity for additional rigorous clinical studies to determine a definitive therapeutic function of vitamin D in IBS based on the literature. The role of vitamin D in the modulation of gut health, particularly in IBS, has garnered significant attention due to its potential impact on both inflammation and the integrity of the gut barrier. Vitamin D, acting as a hormone precursor, is recognised for its crucial involvement in maintaining calcium balance and promoting bone health.

Assessment of Oral Anticoagulation with Vitamin K Antagonists in Patients Living in a Low-Income Country of West Africa

Introduction: Despite the rise of direct oral anticoagulants (DOACs), vitamin K antagonists (VKA) remain the most widely used oral anticoagulants in developing countries. The aim of this study was to estimate the prevalence of good anticoagulation in patients treated with VKA in Lomé and describe associated factors. Methods: This was a cross-sectional study conducted from November 2019 to October 2020 in the cardiology departments of two University teaching hospitals in Lomé (CHU Sylvanus Olympio and CHU Campus), involving patients on VKA for ≥3 months, with a target international normalized ratio (INR) of 2.5 and a therapeutic margin between 2 and 3. The quality of anticoagulation was assessed by the time in therapeutic range (TTR) which was assessed by the Rosendaal method. Good anticoagulation was defined by a TTR > 70%. Results: A total of 344 patients were included (mean age = 58 ± 13.8 years, women = 56.1%). Indications for VKA treatment were represented by venous thromboembolic disease (43.3%), supraventricular arrhythmia (28.2%), severe left ventricular systolic dysfunction (19.8%) and pulmonary hypertension (8.7%). The average TTR was 47.6 ± 20.8%. The rate of good anticoagulation was 17.7%. Factors associated with good anticoagulation were the use of fluindione vs acenocoumarol (OR = 11.17;95% CI: 3.2 - 39.6;p = 0.0002), concomitant low-dose aspirin (OR 4.44;95% CI: 1.4 - 13.9;p = 0.01) and INR monitoring exclusively by the patient himself (OR = 4.92;95% CI: 1.5 - 16.3;p = 0.008). The rate of thromboembolic and hemorrhagic complications was each 2.6% and was not correlated with the quality of anticoagulation. Quality of anticoagulation by VKAs was poor in our practice. Factors associated with good anticoagulation were the use of fluindione vs acenocoumarol, concomitant low-dose aspirin and monitoring of INR exclusively by the patient himself. Conclusion: The quality of oral anticoagulation by VKAs could be improved in our practice by the creation of anticoagulation clinics for better therapeutic education of patients and efficient management of VKA dose, and the use of prescription assistance software.

Vitamin D, Parathyroid Hormone, Insulin Sensitivity and Islet β-Cell Secretory Function in Diabetic Patients from South Kivu in the Democratic Republic of Congo: Cross-Sectional Study

Background: The role of vitamin D and parathyroid hormone in the metabolic profile of type 2 diabetes mellitus in sub-Saharan Africa has not been adequately assessed. The aim of this study was to determine the prevalence of low vitamin D level and secondary hyperparathyroidism and their association with insulin sensitivity and β-cell secretory function among Congolese type 2 diabetics. Methodology: Fasting glycaemia, fasting insulin, 25OH D3 and human parathyroid hormone (hPTH) were measured in one hundred and eighty-four type 2 diabetic patients followed as outpatients in South Kivu. Levels of 25OH D3 65 pg/ml defined low vitamin D and elevated parathyroid hormone levels, respectively. The HOMA model was used to measure insulin sensitivity and β-cell secretory function. Results: Medians (IQR) were 25.3 (20.4 - 32.4) ng/ml for 25OH D3 and 53.7 (38.4 - 115.7) pg/ml for hPTH. 58.7% of diabetics had insulin resistance, 126 (68.5%) had low vitamin D and 80 (43.5%) had hyperparathyroidism. In multivariate analysis, hPTH (partial r = −0.28;p = 0.0002) and 25OH D3 (partial r = 0.16;p = 0.03) showed an independent association with insulin sensitivity after adjustment for body mass index and waist circumference. Finally, hPTH (partial r = 0.27;p = 0.0002) was the sole determinant of β-cell secretory function. Conclusions: This study confirms the high prevalence of low vitamin D level and secondary hyperparathyroidism and their association with insulin resistance and impaired islet β-cell secretory function among Congolese with type 2 diabetes mellitus. Vitamin D and calcium supplementation should be envisaged for cases of deficiency in this region.