目的:探究布地奈德联合维生素K_(3)注射液治疗小儿毛细支气管炎的效果。方法:选择2022年1—12月黔西市人民医院收治的70例毛细支气管炎患儿作为研究对象,采用随机数表法将其分为对照组和观察组,各35例。对照组采用布地奈德治疗,观察组...目的:探究布地奈德联合维生素K_(3)注射液治疗小儿毛细支气管炎的效果。方法:选择2022年1—12月黔西市人民医院收治的70例毛细支气管炎患儿作为研究对象,采用随机数表法将其分为对照组和观察组,各35例。对照组采用布地奈德治疗,观察组采用布地奈德联合维生素K_(3)注射液治疗。比较两组治疗前后白细胞介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)、γ干扰素(interferon-γ,IFN-γ)炎症因子水平,用力肺活量(forced vital capacity,FVC)、第1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))等肺功能指标及效果。结果:两组治疗前IL-6、CRP、IFN-γ、FVC、FEV_(1)水平比较,差异无统计学意义(P>0.05);观察组治疗后IL-6、CRP水平低于对照组,IFN-γ、FVC、FEV_(1)高于对照组,差异有统计学意义(P<0.05)。观察组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。结论:布地奈德联合维生素K_(3)注射液治疗小儿毛细支气管炎的效果显著,不仅能有效降低机体炎症因子水平,还能改善肺功能。展开更多
Background:Hyperglycemia is a typical symptom of diabetes.High glucose induces apoptosis of isletβcells.While autophagy functions in cytoprotection and autophagic cell death.The interaction between autophagy and apop...Background:Hyperglycemia is a typical symptom of diabetes.High glucose induces apoptosis of isletβcells.While autophagy functions in cytoprotection and autophagic cell death.The interaction between autophagy and apoptosis is important in the modulation of the function of isletβcells.Vitamin B3 can induce autophagy and inhibit isletβapoptosis.Method:The mechanism of vitamin B3-mediated protective effect on the function of isletβcells was explored by the method of western blot,immunofluorescence and flow cytometry.Results:In the present study,high glucose stress increased the apoptosis rate,while vitamin B3 reduced the apoptosis rate.The effect of vitamin B3 on autophagy flux under normal and high glucose stress was also investigated.Vitamin B3 increased the number of autophagosomes and increased the light chain(LC)3-II/LC3-I ratio.In contrast,vitamin B3 decreased sequestosome 1(SQSTM1)/p62 protein expression and inhibited the phosphorylation of mammalian ribosomal protein S6 kinaseβ-1(p70S6K/S6K1),which was a substrate of mammalian target of rapamycin(mTOR)under normal and high glucose stress.To further verify the protective effect of vitamin B3 on apoptosis,we treated isletβcell RIN-m5F with autophagy inhibitor 3-methyladenine(3-MA).Vitamin B3 decreased the apoptosis rate under high glucose stress,while the inhibition of apoptosis by vitamin B3 was blocked after adding 3-MA.Conclusion:Our data suggested that vitamin B3 reduced the apoptosis rate ofβcells,possibly through inducing autophagy under high glucose stress.展开更多
文摘目的:探究布地奈德联合维生素K_(3)注射液治疗小儿毛细支气管炎的效果。方法:选择2022年1—12月黔西市人民医院收治的70例毛细支气管炎患儿作为研究对象,采用随机数表法将其分为对照组和观察组,各35例。对照组采用布地奈德治疗,观察组采用布地奈德联合维生素K_(3)注射液治疗。比较两组治疗前后白细胞介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)、γ干扰素(interferon-γ,IFN-γ)炎症因子水平,用力肺活量(forced vital capacity,FVC)、第1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))等肺功能指标及效果。结果:两组治疗前IL-6、CRP、IFN-γ、FVC、FEV_(1)水平比较,差异无统计学意义(P>0.05);观察组治疗后IL-6、CRP水平低于对照组,IFN-γ、FVC、FEV_(1)高于对照组,差异有统计学意义(P<0.05)。观察组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。结论:布地奈德联合维生素K_(3)注射液治疗小儿毛细支气管炎的效果显著,不仅能有效降低机体炎症因子水平,还能改善肺功能。
基金supported by the National-Natural Science Foundation of China(32072334),the General Project of the Education Department of Hunan Province(20C0959)the Changsha Natural Science Foundation(kq2007020).
文摘Background:Hyperglycemia is a typical symptom of diabetes.High glucose induces apoptosis of isletβcells.While autophagy functions in cytoprotection and autophagic cell death.The interaction between autophagy and apoptosis is important in the modulation of the function of isletβcells.Vitamin B3 can induce autophagy and inhibit isletβapoptosis.Method:The mechanism of vitamin B3-mediated protective effect on the function of isletβcells was explored by the method of western blot,immunofluorescence and flow cytometry.Results:In the present study,high glucose stress increased the apoptosis rate,while vitamin B3 reduced the apoptosis rate.The effect of vitamin B3 on autophagy flux under normal and high glucose stress was also investigated.Vitamin B3 increased the number of autophagosomes and increased the light chain(LC)3-II/LC3-I ratio.In contrast,vitamin B3 decreased sequestosome 1(SQSTM1)/p62 protein expression and inhibited the phosphorylation of mammalian ribosomal protein S6 kinaseβ-1(p70S6K/S6K1),which was a substrate of mammalian target of rapamycin(mTOR)under normal and high glucose stress.To further verify the protective effect of vitamin B3 on apoptosis,we treated isletβcell RIN-m5F with autophagy inhibitor 3-methyladenine(3-MA).Vitamin B3 decreased the apoptosis rate under high glucose stress,while the inhibition of apoptosis by vitamin B3 was blocked after adding 3-MA.Conclusion:Our data suggested that vitamin B3 reduced the apoptosis rate ofβcells,possibly through inducing autophagy under high glucose stress.
