采用高效液相色谱技术分析生物体内维生素B_6

For the determination of vitamin B 6 derivatives, a high performance liquid chromatography(HPLC) system composed of a single reversed-phase octadecylsilane column and fluorescence detector with an isocratic elution was proposed. This HPLC system perfectly analyzed pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate together with pyridoxic acid in biological samples. In this method, relatively low fluorescence intensity of pyridoxal 5′-phasphate was improved by converting it to pyridoxic 5′-phosphate with KCN. The analytical procedure is very simple and economical, it is a useful tool for studying vitamin B 6 in animal body.

高效液相色谱荧光法测热带水果中维生素B_6

采用高效液相荧光法测定热带水果中的维生素B6。以酸水解法处理样品，用荧光检测器进行检测，结果表明，该方法检出限浓度为：0．001mg／kg，测定不同样品的相对标准偏差（RSD％）均小于3％，方法的加标回收率为92％～97％。用该方法成功地检测了香蕉、莲雾、榴莲、杨桃、火龙果及龙眼中维生素B6的含量。