Evaluation of the long-term effect of foldable capsular vitreous bodies in severe ocular rupture

AIM:To evaluate the long-term effect of foldable capsular vitreous body(FCVB)in the treatment of severe ocular rupture to provide a practical basis for clinical selection.METHODS:A total of 26 patients(26 eyes),23 men and 3 women,with severe ocular rupture who underwent FCVB implantation between March 2018 and September 2018 were retrospectively analysed.All open ocular wounds located in zone III,with preoperative visual acuity grade IV and above(Snellen less than 4/200).The best corrected visual acuity(BCVA),intraocular pressure(IOP),cornea,anterior chamber,iris,lens,choroid,and retina were evaluated before and after the surgery.The subjective feeling and the location of FCVB were also assessed.RESULTS:The average age of the 26 patients was 36y(20-60y).Postoperative follow-up was from 10 to 14mo.At the end of follow up,BCVA was light perception(LP)in 10 cases,no light perception(NLP)in 13 cases,hand motions(HM)in 3 cases.IOP was 11±5 mm Hg.Corneal degeneration was in 3 cases and corneal endothelial dystrophy was in 7 cases.Shallow anterior chamber was in 8 cases and hyphema was in 8 cases.Organized membrane in the pupil was in 14 cases.Epiphora occurred in 3 cases.FCVB drainage tube exposed in 3 cases.All FCVBs were in their normal location and no rejection occurred.CONCLUSION:FCVB implantation is a long-term effective treatment and may provide a practical selection for severe ocular rupture.

First record of abnormal body coloration in a rockfish Sebastes koreanus(Scorpaenoidei:Sebastidae)from coastal water of China based on morphological characteristics and DNA barcoding

The first record of abnormal body coloration in Sebastes koreanus Kim and Lee,1994,from the Yellow Sea of China,was documented based on morphological characteristics and DNA barcoding.The two rockfish specimens were collected from the coastal waters of Qingdao,China,and the whole body and all fins of them were red.Of the two red-colored rockfish,there were tiny deep red spots on each fin,2 red radial stripes behind and below the eyes and 1 large deep red blotch on the opercula,while the similar stripe and spot patterns are also present in the S.koreanus specimens with normal body coloration.The countable characteristics of the two specimens are in the range of the morphometry of S.koreanus.To further clarify the species identity and taxonomic status of the two specimens,DNA barcode analysis was carried out.The genetic distance between the red-colored rockfish and S.koreanus was 0,and the minimum net genetic distances between the red-colored rockfish and other Sebastes species except for S.koreanus were 3.0%,which exceeds the threshold of species delimitation.The phylogenetic analysis showed that the DNA barcoding sequences of the two red-colored rockfish clustered with the S.koreanus sequences.The above results of DNA barcode analysis also support that the two red-colored rockfish could be identified as the species of S.koreanus.The mechanism of color variation in S.koreanus is desirable for further research and the species could be an ideal model to study the color-driven speciation of the rockfishes.

Structure of vitreous body and its relationship with liquefaction

The aim of the study is to clarify the vitreous body structure and liquefaction phenomena. It was found that when melting a frozen rabbit vitreous body, the gel-sol transition phenomenon occurs and the gel structure is broken. This is almost like the liquefaction of the vitreous body in vivo. We try to clarify the liquefaction phenomenon by using this animal model. The native vitreous body has three dimensional meshwork structures. After liquefaction, it is changed into two parts, namely fiber aggregates and soluble amorphous aggregates. The surface of native vitreous body meshwork is mucopolysacharide, but that of fiber aggregates after liquefaction is changed into connective tissue, which means the conformational change of vitreous body in liquefaction. The soluble proteins after liquefaction were analyzed and identified as crystallin family. It is suggested that the liquefaction is induced by detachment of non-collagenous protein beads containing crystallins, resulting in the collapse of the three dimensional structure to release watery liquid trapped within. And the new gel-sol transition model of vitreous bod is proposed.

Clinical Efficacy and Safety of Foldable Capsular Vitreous Body Implant Surgery in 22 Cases of Silicone Oil-dependent Eyes

Objective This study aimed to evaluate the clinical efficacy and safety of foldable capsular vitreous body(FCVB)implant surgery in silicone oil-dependent eyes.Methods A total of 22 participants with silicone oil-dependent eyes who received treatment with FCVB implant surgery between January 2019 and June 2020 were included in this retrospective study.The intraocular pressure(IOP),best-corrected visual acuity(BCVA),demographic data,and any recorded complications were evaluated.Results The postoperative IOP(12.73±4.20 mmHg)was significantly improved(P=0.03)compared to the preoperative IOP(10.23±3.69 mmHg)(the main endpoint).There was no significant difference(P=0.33)in the final BCVA preoperation and 3rd month postoperation(the secondary endpoint).The most common postoperative complication was hyphema.Other common postoperative complications included corneal opacity,a shallow anterior chamber,and a low IOP.Conclusion FCVB implant surgery is a safe and effective method for treating silicone oil-dependent eyes;however,attention should be paid to the prevention and timely treatment of complications.

Vitreous substitutes: challenges and directions

The natural vitreous body has a fine structure and complex functions. The imitation of the natural vitreous body by vitreous substitutes is a challenging work for both researchers and ophthalmologists. Gases, silicone oil, heavy silicone oil and hydrogels, particularly the former two vitreous substitutes are clinically widely used with certain complications. Those, however, are not real artificial vitreous due to lack of structure and function like the natural vitreous body. This article reviews the situations, challenges, and future directions in the development of vitreous substitutes, particularly the experimental and clinical use of a new artificial foldable capsular vitreous body.

Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment

AIM:To detect the concentrations of reactive oxygen species(ROS),transient receptor potential mucin-1(TRPML1),and autophagy-related(Atg)proteins(LC3-Ⅰ,LC3-Ⅱ,and Beclin1)in vitreous humor of patients with simple rhegmatogenous retinal detachment(RRD).METHODS:RRD patients enrolled as the RRD group,and patients with idiopathic macular hole(IMH)and idiopathic macular epiretinal membrane(IMEM)were enrolled as control group.The levels of ROS,TRPML1,LC3-Ⅰ,LC3-Ⅱ,and Beclin1 in vitreous humor of patients in the RRD and control groups were detected by enzyme-linked immunosorbent assay(ELISA).RESULTS:The RRD group included 28 eyes 28 patients and had a higher concentration of ROS in vitreous humor(631.86±18.05 vs 436.34±108.22 IU/m L,P<0.05).The ROS level in patients with a wide retinal detachment(RD)extent(RD range≥1/2)was higher than that with a narrow RD extent(RD range<1/2,P<0.05).ROS concentration was negatively correlated with RD time(r=-0.46,P=0.01).The expression levels of LC3-Ⅰand Beclin1 significantly decreased in RRD(P<0.05),but there were no correlations with the RD time,RD extent,or macular involvement.CONCLUSION:In eyes with RRD,the concentration of ROS in vitreous humor increases and the expression levels of Atg proteins decrease,reflecting possibly that autophagy is inhibited.

Quantitative Study of Basic Fibroblast Growth Factor in Vitreous with Proliferative Vitreoretinopathy

Objective:To quantitatively study basic fibroblast growth factor(bFGF)in the vitreous ofproliferative vitreoretionopathy(PVR)inorder to understand the role of bFGFin the develop-ment of PVR.Method:High sensitive sandwich enzyme immunoassay technique(ELISA)was used to measure bFGF level in vitreous of normal eyes,the eyes of PVR-Cor pVR-Dgrade,eyes of vitreous hemorrhage and the serum levels of bFGF in PVR-Dpatients.Results:The levels of bFGF in the vitreous were:median5.20ng/L,quartile15.47ng/Lin20normal eyes;median3.12ng/L,quartile10.48ng/Lin35PVR-Ceyes;median46.56ng/L,quartile113.96ng/Lin26pPVR-Deyes;median1.40ng/L,quar-tile6.25ng/Lin25vitreous hemorrhage eyes.The vitreous bFGF level in PVR-D group was significantly higher than that in the normal group,PVR-Cgroup and vitreous hemorrhage group(P<0.01).The mean of serum-bFGFlevel was18.33±3.39ng/L.The vitreous bFGFlevel ofPVR-Dgroup was significantly higher than serum-bFGFlev-el(P<0.01).And the vitreous-bFGFlevel in PVR-Dgroup was significantly higher in larger retinal tear subgroup.Conclusion:The results suggested that bFGF is involved in the development of PVR.Eye Science2000;16:7-10.

基于LoRa的生猪体征监测系统设计与实现

为克服现有生猪体征监测模块功能单一、传输距离受限、功耗大等问题,设计一种以低功耗智能耳标为采集终端的生猪体征监测与异常诊断系统。该系统以无线微控制器为硬件核心,结合猪只体温、心率、运动感知传感器,LoRa和GPRS技术组成的无线传感网络和云服务器构建生猪体征信息采集、健康评估、异常诊断监测平台,提高生猪养殖过程的智能化管理水平。试验表明,该系统运行稳定,能够实时采集、传输、显示、查询生猪体征参数和异常行为,体温相对误差小于1%,心率相对误差小于5%,通信网络丢包率小于5%,传输距离大于3 km,行为辨识结果大于90%,满足中小型规模化生猪养殖场的实际使用需求。可为生猪异常诊断、早期疾病检测治和疫情预防提供科学依据。

异常点云干扰下的车身构件鲁棒性配准方法

点云配准是大型车身构件位姿参数测量的关键方法,但现有算法在大量异常点云干扰下难以配准至有效位姿,从而导致匹配失真,进而无法保证后续机器人作业质量。针对此问题,提出一种能够有效抑制异常点云干扰的车身构件鲁棒性配准算法——鲁棒函数加权方差最小化(RFWVM)算法。建立鲁棒函数加权目标函数,通过施加随迭代次数可变的动态权重来抑制配准过程中异常点云的影响,并由高斯-牛顿法迭代完成刚性转换矩阵的求解。以高铁白车身侧墙、汽车车门框为研究对象的试验结果表明,较经典的最近点迭代(ICP)算法、方差最小化(VMM)算法、加权正负余量方差最小化(WPMAVM)算法和去伪加权方差最小化(DPWVM)算法,所提出的RFWVM算法配准精度更高,能够有效抑制各种异常点云对配准结果的影响,并具有更好的稳定性和鲁棒性,能够有效实现各类车身构件点云的精确配准。

抚顺与铁岭台伸缩仪同步异常特征分析

2019年抚顺与铁岭地震台伸缩仪观测值出现趋势性拉张变化,经过核实,抚顺与铁岭地震台洞体应变均认定为异常并进行跟踪。2020年两项异常开始出现恢复迹象,但自2021年6月开始,抚顺伸缩仪与铁岭伸缩仪再次出现趋势性拉张变化,且两个台站伸缩仪曲线形态具有同步性特征。因此,本文从仪器对比分析、数字滤波及周期分析以及区域长基线GNSS数据结果 3个方面进行分析。结果表明:2021年以来的拉张变化为正常的年变变化,而2019年至今的趋势拉张变化与该区域应力环境变化有关,需持续关注。

基于多目标函数的钢轨打磨廓形优选研究

针对CR400BF和CRH5动车组6~8 Hz主频车体抖动问题,从改善轮轨关系角度出发,分别建立车轮踏面和钢轨廓形样本库,通过确定目标函数和设置边界条件求解得到优选钢轨廓形。建立实参数车辆-轨道刚柔耦合动力学模型和轮轨接触弹塑性有限元模型,通过仿真分析对比优选钢轨廓形和60N廓形的等效锥度、动力学指标和轮轨接触应力。按优选钢轨廓形进行钢轨打磨试验,结果表明:优选钢轨廓形与磨耗车轮踏面匹配时轮轨关系改善,等效锥度小于上限值0.4;与不同磨耗车轮踏面匹配时,优选钢轨廓形动力学指标优于60N,且轮轨接触应力和打磨量均更小;按优选钢轨廓形进行钢轨打磨后,CR400BF、CRH5动车组舒适度、平稳性指标分别减小15.7%、13.1%以上,车体振动加速度主频消除。优选钢轨廓形可有效缓解CR400BF和CRH5动车组车体抖动,优选方法和实施流程可为相关问题研究提供参考。

体医结合背景下高校女大学生异常身体姿态现状调查与运动处方的制定研究

文章从体医结合背景出发,结合运动处方的实施效果和现实意义,通过对高校女大学生异常身体姿态现状调查,了解当代女大学生的生活习惯等对体态的影响,根据大学生自身情况和锻炼需求,借鉴运动处方在全民健身中推广应用的经验与模式,以科学健身为原则,加强对女大学生身体姿态的重视程度,为女大学生提供运动处方的计划方案,有利于提高女大学生的体质健康水平。

S100钙结合蛋白A9与Toll样受体4在糖尿病视网膜病变患者玻璃体液的表达及临床意义

目的了解糖尿病视网膜病变患者玻璃体液中S100钙结合蛋白A9(S100A9)及Toll样受体4(TLR4)的表达并讨论其临床意义。方法选取2022年8月至2023年8月于合肥市第二人民医院行手术治疗的患者40例。研究组为糖尿病视网膜病变需行玻璃体切割手术治疗的20例患者,对照组为特发性黄斑裂孔或黄斑前膜需行玻璃体切除手术的20例患者。收集患者玻璃体液,用ELISA法测定其S100A9及TLR4蛋白浓度。结果研究组玻璃体液S100A9浓度为(5.95±1.55)μg/L高于对照组(2.19±0.76)μg/L,差异有统计学意义(P<0.05);研究组玻璃体液TLR4浓度为(1.54±0.79)μg/L,高于对照组(0.34±0.18)μg/L,差异有统计学意义(P<0.05);研究组玻璃体液S100A9与TLR4呈正相关(r=0.72,P<0.05)。结论糖尿病视网膜病变患者玻璃体液中S100A9及TLR4水平增高,提示炎症参与其发病过程。

折叠式人工玻璃体球囊在硅油依赖眼中的临床应用研究

目的探讨折叠式人工玻璃体球囊(FCVB)植入治疗硅油依赖眼的临床效果。方法回顾分析2021年4月—2022年2月就诊于复旦大学附属眼耳鼻喉科医院且因硅油依赖眼行FCVB植入术的患者临床资料,评价FCVB植入的效果。结果本研究共纳入104例(104眼),其中男性74例、女性30例。平均年龄为(36.00±18.25)岁(3~68岁)。所有眼均为硅油依赖眼,包括眼外伤94眼(90.4%),急性视网膜坏死3眼,复杂性视网膜脱离7眼。平均随访时间为(337.50±102.03)d(180~550 d)。所有患者行25 G玻璃体切除联合FCVB植入术,残留的视网膜均成功复位,术前眼压、眼轴及最佳矫正视力与术后相比,差异均无统计学意义。所有患眼FCVB植入均顺利,未发生眼内炎、引流阀暴露及球囊破裂等严重并发症。14眼(13.5%)术后角膜混浊程度加重。15眼(14.4%)术后发生前房积血,再次行前房冲洗术。5眼(4.8%)因虹膜缺失,球囊向前移位,再次行前房成形术。结论FCVB植入治疗硅油依赖眼,经短期随访,可以维持眼球形态,防止眼球萎缩,但其长远效果仍需进一步长期随访研究。

血清和玻璃体中miR-126和miR-325与增生性玻璃体视网膜病变严重程度的关系

目的:探讨血清和玻璃体中miR-126和miR-325与增生性玻璃体视网膜病变(PVR)严重程度的关系。方法:回顾性研究。选取2019-10/2022-10在本院治疗的PVR患者100例100眼。按照视网膜病变程度分为轻度组42眼和重度组58眼。选取同期因眼外伤在本院进行玻璃体切除术无视网膜病变的患者30例30眼为对照组。采用荧光定量PCR检测血清和玻璃体中miR-126和miR-325表达水平;ELISA检测血清、玻璃体中转化生长因子-β(TGF-β)、血小板衍生生长因子(PDGF)、血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)水平;Pearson法分析血清和玻璃体中miR-126和miR-325水平与TGF-β、PDGF、VEGF、TNF-α水平的相关性;采用Logistic多因素分析影响发生重度PVR的因素。结果:PVR患者血清和玻璃体中miR-126水平较对照组降低,且重度组低于轻度组(均P<0.05);miR-325水平较对照组升高,且重度组高于轻度组(均P<0.05)。重度组患者血清和玻璃体中TGF-β、PDGF、VEGF、TNF-α水平较轻度组均上升(均P<0.05)。PVR患者血清和玻璃体中miR-126水平与miR-325、TGF-β、VEGF、TNF-α、PDGF水平均呈负相关(均P<0.05),miR-325与TGF-β、VEGF、TNF-α、PDGF水平均呈正相关(均P<0.05)。Logistic回归分析显示,血清和玻璃体中miR-325、TGF-β、PDGF、TNF-α均是发生重度PVR的危险因素,miR-126是保护因素(P<0.05)。结论:随PVR疾病的加重,患者血清和玻璃体中miR-126表达降低,miR-325表达升高,且与TGF-β、TNF-α、VEGF、PDGF具有相关性。

糖尿病黄斑水肿患者玻璃体腔注射康柏西普治疗效果的影响因素分析

目的探讨糖尿病黄斑水肿(DME)患者玻璃体腔注射康柏西普治疗效果的影响因素。方法选取2019年6月至2021年5月于江西医学高等专科学校第一附属医院就诊的127例DME患者作为研究对象,全部患者均接受玻璃体腔注射康柏西普治疗,治疗7 d后参照相关标准评估患者治疗效果;设计基线资料调查表,询问并记录患者基线资料,将可能的因素纳入,经logistic回归分析找出DME患者玻璃体腔注射康柏西普治疗无效的影响因素。结果127例DME患者经玻璃体腔注射康柏西普治疗,有36例无效,无效率为28.35%;单因素分析结果提示,吸烟史、糖化血红蛋白(HbA1c)水平、低密度脂蛋白(LDL)水平、高密度脂蛋白(HDL)水平升高是DME患者玻璃体腔注射康柏西普治疗效果的相关因素(P<0.05);多因素分析结果提示,吸烟史(β=0.900,OR=2.461,95%CI:1.118~5.413)、HbA1c水平升高(β=1.106,OR=3.023,95%CI:1.753~5.213)、LDL水平升高(β=0.576,OR=1.780,95%CI:1.167~2.713)是DME患者玻璃体腔注射康柏西普治疗无效的影响因素(OR>1,P<0.05);HDL水平升高(β=-3.699,OR=0.025,95%CI:0.003~0.191)是DME患者玻璃体腔注射康柏西普治疗无效的保护因素(OR<1,P<0.05)。结论DME患者玻璃体腔注射康柏西普治疗无效可能受吸烟史、HbA1c水平升高、LDL水平升高、HDL水平降低影响。

从肺论治内障眼病初探

内障眼病外无见症,在现代眼科中主要包括眼后段疾病,其病因病机十分复杂。“目病常统于肺”是阐述内障眼病病因病机的重要理论。肺为相傅之官,主一身之气,在眼的生理功能和病因病机中具有关键作用。肺对眼内气、血、津液有疏通和调节作用,尤以肺气宣降为要,在视网膜血氧调节中可见一斑;玻璃体属于手太阴肺经,手太阴肺经为阴经气化活动的开始,玻璃体在内眼中亦是如此。在现代研究中也可发现,玻璃体微粒在内障眼病中起着调节和信使的作用。本文分别从理论研究和现代研究2个方面,分析从肺论治内障眼病的依据,着重从经络和藏象的角度进行探讨。

循证护理对视网膜脱离患者术后康复的影响

目的探讨循证护理对孔源性视网膜脱离术后康复的影响。方法选取厦门大学附属第一医院眼科2021年10月—2023年10月行玻璃体切除+硅油植入手术治疗的单纯孔源性视网膜脱离患者72例,采用单纯随机抽样分为2组,各36例。对照组给予常规护理,观察组给予循证护理,对比2组患者术后1个月视力康复情况、用药准确性、体位依从性、术后不良反应情况。结果相较于对照组,观察组术后1个月视力>0.10者多(P<0.05),同时患者用药准确性高,患者体位正确性较好。观察组术后并发症发生率(2.78%)低于对照组(22.22%)(P<0.05)。结论循证护理比常规护理可能更有利于孔源性视网膜脱离患者术后的康复。

Characteristic analysis on susceptibility weighted imaging of intravitreous foreign body Of autologous eyelashes in rabbits

Objective： To explore the characteristics of susceptibility weighted imaging （SWI） of the intravitreous foreign body of autologous eyelashes in rabbits. Methods： A total of 12 New Zealand white rabbits, either sex, weighing 2.5-3.5 kg, and provided by the Experimental Animal Center of Henan Province were employed in this study. For each rabbit, 5 autologous eyelashes （1 cm in length and 0.2-0.3 mm in diameter） were implanted into the right ocular vitreum, while the left control ocular vitreum received sham operation but nothing was implanted. SWI sequential test was made 2 hours postoperatively. Then the rabbits were killed and the specimens of the vitreous bodies of the rabbits were obtained. Hematoxylin and eosin staining and histological examinations were performed. Results： The autologous eyelashes in 8 ocular vitreums of rabbits showed linear low signal intensity on the magni-tude images and susceptibility weighted images, but linear high signal intensity on the phase images. Among the 12 experimental rabbits, 5 eyelashes in the right vitreum were completely shown in 3 rabbits, partly shown in 5 rabbits （2 eyelashes shown in 3 rabbits and 3 eyelashes shown in 2 rabbits）, and not shown in 4 rabbits. Conelusions： SWI of the foreign body ofintravitreous autologous eyelashes in rabbits has its own characteristics. The combined application of SWI sequential magnitude images, susceptibility weighted images and phase images is helpful to the detection and diagnosis of intravitreous autologous eyelashes in rabbits.

体位改变结合胎头手转对胎位异常产妇分娩方式的影响分析

目的探讨体位改变联合胎头手转在胎位异常产妇分娩方式中的意义。方法单纯随机选取2021年10月—2023年10月滕州市中心人民医院接收的120例产妇为研究对象,根据随机数字法分成对照组(60例)与观察组(60例)。对照组产妇接受体位改变,观察组产妇接受体位改变+胎头手转。比较两组产妇胎位矫正成功率、剖宫产率、新生儿Apgar评分及分娩时间。结果观察组的胎位矫正成功率为65.00%,高于对照组的43.33%;剖宫产率为35.00%,低于对照组的56.67%,差异有统计学意义(χ^(2)=5.673、5.673,P均<0.05)。观察组新生儿Apgar评分优于对照组,差异有统计学意义(P<0.05);观察组分娩时间短于对照组,差异有统计学意义(P<0.05)。结论联合应用体位改变与胎头手转,可以提高胎位异常产妇的胎位矫正成功率,进而降低剖宫产率,缩短产妇分娩时间。