目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单...目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单个核细胞(PBMC);病毒性乙型肝炎慢性重度和重型患者标本33例,病毒性乙型肝炎慢性轻度、中度共25例,健康对照25例分离其外周血白细胞(WBC)。用一期冻融法测量其PCA。采用免疫组化的方法检测hfgl2的表达。结果:病毒性乙型肝炎慢性重型患者PBMC的PCA较健康对照高出20倍。WBC的PCA在以下各组(健康对照组、病毒性乙型肝炎慢性轻中度组、病毒性乙型肝炎慢性重度和重型组)呈逐步上升过程,但组间差异无显著性意义。PCA在病毒性乙型肝炎慢性重型组患者PBMC中的表达显著高于其在WBC的表达。PCA在健康对照组PBMC和WBC的表达无显著差异,hfgl2在病毒性乙型肝炎慢性重型患者PBMC中的表达水平显著高于其在健康对照组中的表达。结论:病毒性乙型肝炎慢性重型患者PBMC中的PCA和hfgl2表达均显著升高,且其表达与疾病严重程度相关,hfgl2表达的强度(阳性细胞数)与PCA的升高呈正比相关。展开更多
Background: Coronavirus disease 2019 (COVID-19) is a recent global health crisis. One of the major issues of COVID-19 is its unpredictable manifestations and serious outcomes. Many hematological parameters are thought...Background: Coronavirus disease 2019 (COVID-19) is a recent global health crisis. One of the major issues of COVID-19 is its unpredictable manifestations and serious outcomes. Many hematological parameters are thought to change dramatically during the course of the disease. These include white blood cells, red blood cells, and platelets. This study aimed at evaluating certain laboratory results;peripheral blood lymphopenia, relative neutrophilia, high neutrophil-lymphocyte ratio, and elevated C-reactive protein as potential laboratory markers of COVID-19 in Eastern Sudanese patients. Methods: We, retrospectively, aimed at the evaluation of peripheral blood leucocytes count, neutrophil-lymphocyte ratio NLR and C-reactive protein (CRP) levels in confirmed COVID-19 eastern Sudanese patients during the course of the disease. Results: The mean total leucocytes count, % neutrophils count, absolute neutrophils count and C-reactive protein (CRP) were significantly higher (P. value = 0.000) in COVID-19 patients than in the control group while the mean % lymphocytes count and % mixed cells count were found to be significantly lower in COVID-19 patients than in the control group (P. value 0.000). Conclusion: Peripheral blood leucocyte alterations (simultaneous presence of lymphopenia, relative neutrophilia and high neutrophil lymphocyte ratio (NLR) along with elevated CRP levels may be valuable biomarkers associated with COVID-19 in Port Sudan city, Red Sea state, Sudan. These markers might be important in prediction, inspection of disease progression and prognosis.展开更多
文摘目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单个核细胞(PBMC);病毒性乙型肝炎慢性重度和重型患者标本33例,病毒性乙型肝炎慢性轻度、中度共25例,健康对照25例分离其外周血白细胞(WBC)。用一期冻融法测量其PCA。采用免疫组化的方法检测hfgl2的表达。结果:病毒性乙型肝炎慢性重型患者PBMC的PCA较健康对照高出20倍。WBC的PCA在以下各组(健康对照组、病毒性乙型肝炎慢性轻中度组、病毒性乙型肝炎慢性重度和重型组)呈逐步上升过程,但组间差异无显著性意义。PCA在病毒性乙型肝炎慢性重型组患者PBMC中的表达显著高于其在WBC的表达。PCA在健康对照组PBMC和WBC的表达无显著差异,hfgl2在病毒性乙型肝炎慢性重型患者PBMC中的表达水平显著高于其在健康对照组中的表达。结论:病毒性乙型肝炎慢性重型患者PBMC中的PCA和hfgl2表达均显著升高,且其表达与疾病严重程度相关,hfgl2表达的强度(阳性细胞数)与PCA的升高呈正比相关。
文摘Background: Coronavirus disease 2019 (COVID-19) is a recent global health crisis. One of the major issues of COVID-19 is its unpredictable manifestations and serious outcomes. Many hematological parameters are thought to change dramatically during the course of the disease. These include white blood cells, red blood cells, and platelets. This study aimed at evaluating certain laboratory results;peripheral blood lymphopenia, relative neutrophilia, high neutrophil-lymphocyte ratio, and elevated C-reactive protein as potential laboratory markers of COVID-19 in Eastern Sudanese patients. Methods: We, retrospectively, aimed at the evaluation of peripheral blood leucocytes count, neutrophil-lymphocyte ratio NLR and C-reactive protein (CRP) levels in confirmed COVID-19 eastern Sudanese patients during the course of the disease. Results: The mean total leucocytes count, % neutrophils count, absolute neutrophils count and C-reactive protein (CRP) were significantly higher (P. value = 0.000) in COVID-19 patients than in the control group while the mean % lymphocytes count and % mixed cells count were found to be significantly lower in COVID-19 patients than in the control group (P. value 0.000). Conclusion: Peripheral blood leucocyte alterations (simultaneous presence of lymphopenia, relative neutrophilia and high neutrophil lymphocyte ratio (NLR) along with elevated CRP levels may be valuable biomarkers associated with COVID-19 in Port Sudan city, Red Sea state, Sudan. These markers might be important in prediction, inspection of disease progression and prognosis.
