采用"点击化学"的合成方法制备了一种混合配体的辛基-羧基共同键合硅胶(OCS)材料,经元素分析和红外光谱表征,证明了辛基和羧基官能团均已成功键合到硅胶表面。将其作为混合模式反相弱阳离子交换(RP/W C X)的固定相填料,以3种...采用"点击化学"的合成方法制备了一种混合配体的辛基-羧基共同键合硅胶(OCS)材料,经元素分析和红外光谱表征,证明了辛基和羧基官能团均已成功键合到硅胶表面。将其作为混合模式反相弱阳离子交换(RP/W C X)的固定相填料,以3种同系物阳离子表面活性剂作为探针分子,定量研究了该固定相的混合模式保留机理,考察了溶质保留因子与盐浓度和溶质亚甲基数目的关系,验证了混合模式固定相的单点和两点保留机理的数学模型,并进一步将其应用于一系列标准碱性混合物的分离。结果表明:两点保留机理更加符合实验的结果;根据混合模式两点保留机理的数学方程,可以得到单一的疏水或离子交换作用力对总保留的影响,对混合模式色谱的实际分离应用提供了有价值的参考。本文建立了反相弱阳离子交换混合模式OCS固定相的保留机理的定量模型,并证明了该固定相在碱性化合物的分离中具有很大的应用前景。展开更多
Objective:To study the value of the differentially expressed proteins from primary and recurrent ovarian cancer serum for early diagnosis of primary and recurrent ovarian cancer.Methods: WCX kit(Bruker Daltonics GraBH...Objective:To study the value of the differentially expressed proteins from primary and recurrent ovarian cancer serum for early diagnosis of primary and recurrent ovarian cancer.Methods: WCX kit(Bruker Daltonics GraBH) and matrix-assisted laser desorption/ionization time-offlight mass spectrometry(MALDI-TOF-MS]technology were used to detect serum samples from 49 patients with primary ovarian cancer and 21 patients with recurrent disease.Results:In the mass range(Mr) from 1 000 to 12 000 Da,eight differentially expressed protein peaks were screened from primary ovarian cancer serum.Among them,four protein peaks with Mr 1 457,1 857,2 202, 7 761 were lowly expressed and the others with Mr 2 946,5 333,5 859,5 901 were highly expressed. Ten differentially expressed protein peaks were screened from recurrent ovarian cancer serum. Among them.1 944,1 980,2 080,2 661,2 993,4 450,4 659,5 359 Da protein expressions were increased significantly,and 1897,7868 Da protein expressions were decreased significantly.The pattern of primary ovarian cancer was applied to 8 early-stage ovarian cancer serum samples, and 7 serum samples were successfully predicted with the accuracy of 87.5%.The pattern of recurrent ovarian cancer was applied to 9 without pelvic:or abdominal mass recurrent ovarian cancer serum samples,and 8 serum samples were successfully predicled with the accuracy of 88.9%.Conclusions:Combination of MALDI-TOF-MS and WCX kit technology can directly screen the diferrential expressed protein from primary and recurrent ovarian cancer serum.They have clinical significance for enhancement of sensitivity and specificity of ovarian cancer diagnosis.展开更多
Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based pu...Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps.展开更多
文摘采用"点击化学"的合成方法制备了一种混合配体的辛基-羧基共同键合硅胶(OCS)材料,经元素分析和红外光谱表征,证明了辛基和羧基官能团均已成功键合到硅胶表面。将其作为混合模式反相弱阳离子交换(RP/W C X)的固定相填料,以3种同系物阳离子表面活性剂作为探针分子,定量研究了该固定相的混合模式保留机理,考察了溶质保留因子与盐浓度和溶质亚甲基数目的关系,验证了混合模式固定相的单点和两点保留机理的数学模型,并进一步将其应用于一系列标准碱性混合物的分离。结果表明:两点保留机理更加符合实验的结果;根据混合模式两点保留机理的数学方程,可以得到单一的疏水或离子交换作用力对总保留的影响,对混合模式色谱的实际分离应用提供了有价值的参考。本文建立了反相弱阳离子交换混合模式OCS固定相的保留机理的定量模型,并证明了该固定相在碱性化合物的分离中具有很大的应用前景。
文摘Objective:To study the value of the differentially expressed proteins from primary and recurrent ovarian cancer serum for early diagnosis of primary and recurrent ovarian cancer.Methods: WCX kit(Bruker Daltonics GraBH) and matrix-assisted laser desorption/ionization time-offlight mass spectrometry(MALDI-TOF-MS]technology were used to detect serum samples from 49 patients with primary ovarian cancer and 21 patients with recurrent disease.Results:In the mass range(Mr) from 1 000 to 12 000 Da,eight differentially expressed protein peaks were screened from primary ovarian cancer serum.Among them,four protein peaks with Mr 1 457,1 857,2 202, 7 761 were lowly expressed and the others with Mr 2 946,5 333,5 859,5 901 were highly expressed. Ten differentially expressed protein peaks were screened from recurrent ovarian cancer serum. Among them.1 944,1 980,2 080,2 661,2 993,4 450,4 659,5 359 Da protein expressions were increased significantly,and 1897,7868 Da protein expressions were decreased significantly.The pattern of primary ovarian cancer was applied to 8 early-stage ovarian cancer serum samples, and 7 serum samples were successfully predicted with the accuracy of 87.5%.The pattern of recurrent ovarian cancer was applied to 9 without pelvic:or abdominal mass recurrent ovarian cancer serum samples,and 8 serum samples were successfully predicled with the accuracy of 88.9%.Conclusions:Combination of MALDI-TOF-MS and WCX kit technology can directly screen the diferrential expressed protein from primary and recurrent ovarian cancer serum.They have clinical significance for enhancement of sensitivity and specificity of ovarian cancer diagnosis.
基金Supported by the National Natural Science Foundation of China(No.30600458)
文摘Peptides in shrimp hemolymph play an important role in the innate immune response.Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection.We used magnetic bead-based purification(ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) to characterize shrimp hemolymph peptides.Shrimp serum and plasma were used as the source of samples for comparative analysis,and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis.To screen potential specific biomarkers in serum of immune-challenged shrimps,we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps.The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software.Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide(LPS)-infection.The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%.Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph,and will help to enable a better understanding of the innate immune response of shrimps.
