The objective of this study is to investigate the immune-enhancing ability of viable and heat-killed Weissella cibaria JW15(JW15)isolated from Kimchi in RAW 264.7 macrophages.The immune effects were evaluated by measu...The objective of this study is to investigate the immune-enhancing ability of viable and heat-killed Weissella cibaria JW15(JW15)isolated from Kimchi in RAW 264.7 macrophages.The immune effects were evaluated by measuring the production of NO,cytokines,inflammatory enzyme,and activation of NF-κB.Viable JW15 executed higher activity on stimulating the release of TNF-αas well as activating NF-κB compared to that of heatkilled JW15.Additionally,viable and heat-killed JW15 significantly increased the production of NO,IL-6 and TNF-αmore than that of Lactobacillus rhamnosus GG(LGG).Furthermore,viable JW15 induced higher production of i NOS compared with that of viable LGG.Collectively,our finding indicates that viable JW15 had similar,if not more,immune-enhancing activities as heat-killed JW15.In addition,viable JW15 had higher immune-enhancing activity than commercial strain LGG.Therefore,viable JW15 has the potential to be used as a functional food to improve the host immune response.展开更多
The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Ta...The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Taguchi's orthogonal array. Four metabolism influencing factors viz. sucrose, yeast extract, K2HPO4 and Tween80 were selected to optimize dextransucrase production by W. confusa Cab3 using fractional factorial design of Taguchi methodology. Based on the influence of interaction components of fermentation, least significant factors of individual level have higher interaction severity index and vice versa for enzyme production from Weissella confusa Cab3. Sucrose and yeast extract were found to be the most significant factors which positively influenced the dextransucrase production. The optimized medium composition consisted of sucrose—5%;yeast extract—2%;K2HPO4—1.0%;Tween80—0.5%, based on Taguchi orthogonal array method. The optimized composition gave an experimental value of dextransucrase activity of 17.9 U/ml at shake flask level which corresponded well with the predicted value of 17.54 U/ml by the model. The optimized medium by Taguchi method gave significant (3 fold) enhancement of dextransucrase activity as compared to unoptimised enzyme activity of 6.0 U/ml. The dextransucrase production was scaled up in lab scale bioreactor resulting in further enhancement of enzyme activity (22.0 U/ml).展开更多
Maize straw,an abundant agricultural residue,has potential as an eco-friendly resource.The aim of this study was to investigate the struc-ture and effect on Bifidobacterium longum of exopolysaccharide produced by Weis...Maize straw,an abundant agricultural residue,has potential as an eco-friendly resource.The aim of this study was to investigate the struc-ture and effect on Bifidobacterium longum of exopolysaccharide produced by Weissella cibaria using maize straw as a carbon source.In this work,an exopolysaccharide-producing strain was isolated from vegetable juice,which was identified as W.cibaria.Our results demonstrated that exopolysaccharide produced by W.cibaria is a linear glucan containing anα-1,6 glycosidic bond with a molecular weight of 9462 Da.In addition,scanning electron microscopy of exopolysaccharide from W.cibaria revealed the flake structures.In vitro assays showed that the exopolysaccharide from W.cibaria has the ability to stimulate the proliferation of B.longum strain and its metabolism to produce acetic acid.This work provides a scientific reference for the potential application of maize straw and a new insight into an eco-friendly carbon source to produce exopolysaccharide.展开更多
基金support of"Cooperative Research Program for Agriculture Science&Technology Development(Project No.PJ01283407)"Rural Development Administration,Republic of Koreasupported by Post-Doctoral Fellowship Program funded by the Ministry of Education of the Republic of Korea through the Chungbuk National University in 2019。
文摘The objective of this study is to investigate the immune-enhancing ability of viable and heat-killed Weissella cibaria JW15(JW15)isolated from Kimchi in RAW 264.7 macrophages.The immune effects were evaluated by measuring the production of NO,cytokines,inflammatory enzyme,and activation of NF-κB.Viable JW15 executed higher activity on stimulating the release of TNF-αas well as activating NF-κB compared to that of heatkilled JW15.Additionally,viable and heat-killed JW15 significantly increased the production of NO,IL-6 and TNF-αmore than that of Lactobacillus rhamnosus GG(LGG).Furthermore,viable JW15 induced higher production of i NOS compared with that of viable LGG.Collectively,our finding indicates that viable JW15 had similar,if not more,immune-enhancing activities as heat-killed JW15.In addition,viable JW15 had higher immune-enhancing activity than commercial strain LGG.Therefore,viable JW15 has the potential to be used as a functional food to improve the host immune response.
文摘The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Taguchi's orthogonal array. Four metabolism influencing factors viz. sucrose, yeast extract, K2HPO4 and Tween80 were selected to optimize dextransucrase production by W. confusa Cab3 using fractional factorial design of Taguchi methodology. Based on the influence of interaction components of fermentation, least significant factors of individual level have higher interaction severity index and vice versa for enzyme production from Weissella confusa Cab3. Sucrose and yeast extract were found to be the most significant factors which positively influenced the dextransucrase production. The optimized medium composition consisted of sucrose—5%;yeast extract—2%;K2HPO4—1.0%;Tween80—0.5%, based on Taguchi orthogonal array method. The optimized composition gave an experimental value of dextransucrase activity of 17.9 U/ml at shake flask level which corresponded well with the predicted value of 17.54 U/ml by the model. The optimized medium by Taguchi method gave significant (3 fold) enhancement of dextransucrase activity as compared to unoptimised enzyme activity of 6.0 U/ml. The dextransucrase production was scaled up in lab scale bioreactor resulting in further enhancement of enzyme activity (22.0 U/ml).
基金supported by Fujian Agriculture and Forestry University(No.72202200901),China.
文摘Maize straw,an abundant agricultural residue,has potential as an eco-friendly resource.The aim of this study was to investigate the struc-ture and effect on Bifidobacterium longum of exopolysaccharide produced by Weissella cibaria using maize straw as a carbon source.In this work,an exopolysaccharide-producing strain was isolated from vegetable juice,which was identified as W.cibaria.Our results demonstrated that exopolysaccharide produced by W.cibaria is a linear glucan containing anα-1,6 glycosidic bond with a molecular weight of 9462 Da.In addition,scanning electron microscopy of exopolysaccharide from W.cibaria revealed the flake structures.In vitro assays showed that the exopolysaccharide from W.cibaria has the ability to stimulate the proliferation of B.longum strain and its metabolism to produce acetic acid.This work provides a scientific reference for the potential application of maize straw and a new insight into an eco-friendly carbon source to produce exopolysaccharide.
