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Efficacy of Vacuum-Assisted Closure (VAC) in Wound Healing
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作者 Zeeshan Ahmed Najam Husain +1 位作者 Shahd Nour Sook Han Yee 《Surgical Science》 2019年第6期173-215,共43页
Introduction: Management of wounds is one of the commonest, often quite challenging as well, in surgical practice. Dressing is an essential component of wound management. Given its importance in achieving wound healin... Introduction: Management of wounds is one of the commonest, often quite challenging as well, in surgical practice. Dressing is an essential component of wound management. Given its importance in achieving wound healing and its complications, methods of dressing have been under constant review since time immemorial. Many innovative techniques of wound dressing have been introduced for quick and problem-free wound healing. Vacuum Assisted Closure (VAC) is one of such methods. Objective: To determine whether VAC can help in reducing the healing time of open wounds. Study Design: Quasi-experimental study. Setting: Department of General Surgery, Pakistan Institute of Medical Sciences, Islamabad. Patients and Methods: 120 patients with open wounds, surgical or accidental, were included in the study. They were alternatively assigned to either VAC (Group I) 2 or WMD {wet to moist dressing} (Group II). In both groups, the wounds were assessed for surface area, depth and percentage of granulation every 48 hours for 15 days. Mean change in above wound parameters at the end of study period was calculated. The difference between the two groups was statistically analyzed by applying student T test. P value less than 0.05 was considered significant. Results: In Group I, at the end of the study period, mean reduction in the wound surface area and volume was 39.4% and 60.3% respectively, while granulation tissue formation was 59.7%. In Group II, the respective figures were 26.9%, 38.2% and 24.5%. The difference between the two groups was statistically significant (P Conclusion: VAC was more effective than WMD in terms of reduction in wound surface, depth and degree of granulation tissue formation. VAC thus can be usefully employed for achieving speedy wound healing. 展开更多
关键词 WOUND VAC NPWT wmd3
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amiRNA分子设计及其离体合成策略 被引量:2
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作者 叶梅霞 崔东清 +4 位作者 李昊 王静澄 刘军梅 张志毅 安新民 《中国生物工程杂志》 CAS CSCD 北大核心 2010年第9期92-97,共6页
详细介绍了基于WMD3(Web MicroRNA Designer 3)软件平台的amiRNA(artificial microRNA)分子自动设计方法及其离体合成策略。应用网络在线设计时,只需输入目的基因靶序列相关信息后便可获得候选amiRNA。根据选定的最佳amiRNA,可得到4条含... 详细介绍了基于WMD3(Web MicroRNA Designer 3)软件平台的amiRNA(artificial microRNA)分子自动设计方法及其离体合成策略。应用网络在线设计时,只需输入目的基因靶序列相关信息后便可获得候选amiRNA。根据选定的最佳amiRNA,可得到4条含有amiRNA以及载体中miRNA两侧序列的寡聚核苷酸序列。重叠延伸PCR合成策略可以以这4条序列以及根据质粒模板设计的A、B两段序列作为引物,扩增出目标amiRNA。同样尿嘧啶切除的策略也可合成amiRNA,但这些引物序列需做适当调整变动。此外,还介绍了基于特异引物退火的amiRNA合成策略,该策略可保证amiRNA分子能够一步PCR合成,合成后的amiRNA表达盒可通过合适的限制性位点被克隆至目的载体中的相应位点。可以预见,这种amiRNA分子设计的科学性与合成策略的精确性将会使amiRNAi技术在生物基因功能分析中发挥更加重要的作用,对生命科学研究产生深远的影响。 展开更多
关键词 wmd3 amiRNA 分子设计 合成策略
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