Research Progress on the Mechanism of Correlation Between Vitamin D and Thyroid Cancer

Vitamin D is a kind of fat-soluble vitamin,which is mainly involved in the metabolism of calcium and bone in the human body.As a metabolic substance,it also has a certain impact on the cellular microenvironment,and vitamin D also inhibits the proliferation of tumor cells.25(OH)D is considered the best index to evaluate the vitamin D level in the human body because of its relatively stable characteristics in the circulation.Thyroid cancer is a common malignant tumor that develops from malignant thyroid nodules.A large number of studies have found that the lower the serum 25(OH)D level,the higher the risk of thyroid nodules.A large number of studies have found that the lower the serum 25(OH)D level,the higher the risk of thyroid nodules.

白藜芦醇对结肠癌大鼠果蝇WNT同源基因/β-连环蛋白信号通路的影响

目的探讨白藜芦醇对结肠癌大鼠果蝇WNT同源基因/β-连环蛋白(Wnt/β-catenin)信号通路的影响。方法用N-甲基-N-亚硝基脲灌肠构建结肠癌模型。将造模成功大鼠随机分为模型组、对照组和低、高剂量实验组,每组15只;另取15只正常大鼠作为空白组。空白组和模型组均灌胃给予0.9%NaCl_(2) mL,qd;低、高剂量实验组分别灌胃给予2.5和5.0 mg·mL^(-1)白藜芦醇溶液2 mL,qd;对照组尾静脉注射给予5.44 mg·mL^(-1)奥沙利铂溶液1 mL,每3周1次。5组大鼠均连续给药12周。比较各组的抑瘤率,用蛋白质印迹法测定肿瘤组织Wnt/β-catenin信号通路相关蛋白的表达水平。结果治疗后,低、高剂量实验组和对照组的抑瘤率分别为(35.42±3.71)%,(47.52±4.26)%和(57.24±6.04)%,低、高剂量实验组的抑瘤率与对照组比较,差异均有统计学意义(均P <0.05)。治疗后,低、高剂量实验组和对照组、模型组、空白组肿瘤组织的β-catenin相对表达量分别为1.74±0.23,1.38±0.17,1.14±0.15,2.53±0.34和0.58±0.08,TCF-4相对表达量分别为1.89±0.25,1.67±0.23,1.25±0.17,2.62±0.36和0.64±0.09,C-myc相对表达量分别为1.82±0.22,1.53±0.21,1.21±0.14,2.59±0.37和0.61±0.07,Cyclin D1相对表达量分别为1.78±0.21,1.57±0.22,1.33±0.18,2.49±0.35和0.52±0.07。模型组的上述指标与低、高剂量实验组和空白组、对照组比较,差异均有统计学意义(均P <0.05)。结论白藜芦醇能够剂量依赖性地抑制结肠癌大鼠肿瘤组织生长,其作用可能与抑制Wnt/β-catenin信号通路激活有关。