De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regene...De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regenera-tion from the wounded sites of detached leaves or stems,or in in-vitro tissue culture,e.g.organ regeneration from cal-lus.In this review,we summarize recent advances in research on the molecular mechanism of de novo organ regen-eration,focusing on the role of the WUSCHEL-RELATED HOMEOBOX11(WOX11)gene in the model plant Arabidopsis thaliana.WOX11 is a direct target of the auxin signaling pathway,and it is expressed in,and regulates the establish-ment of,the founder cell during de novo root regeneration and callus formation.WOX11 activates the expression of its target genes to initiate root and callus primordia.Therefore,WOX11 links upstream auxin signaling to downstream cell fate transition during regeneration.We also discuss the role of WOX11 in diverse species and its evolution in plants.展开更多
De novo root regeneration(DNRR) has wide applications in agriculture such as those related to cutting technology. Detached Arabidopsis thaliana leaf explants can regenerate adventitious roots without added hormones. T...De novo root regeneration(DNRR) has wide applications in agriculture such as those related to cutting technology. Detached Arabidopsis thaliana leaf explants can regenerate adventitious roots without added hormones. The regenerative ability is highly dependent on the developmental status of the leaf. An immature leaf has a higher regenerative ability, while a mature leaf is difficult to regenerate. Using RNASeq analysis, we showed that the expression levels of many genes, including those in the auxin network,changed during leaf maturation. Particularly, the expression levels of many YUCCA(YUC) genes in the auxin biosynthesis pathway are responsive to leaf maturation. Overexpression of YUC1 in the yuc-1 D dominant mutant rescued the rooting defects caused by leaf maturation. In addition, YUC4 expression levels were also affected by circadian rhythms. The regenerative ability was reduced in both immature and mature mutant leaf explants from the new wuschel-related homeobox 11-3(wox11-3) and wox12-3 mutant alleles created by the CRISPR/Cas9 method. Overall, the transcriptome and genetic data, together with the auxin concentration analysis, indicate that the ability to upregulate auxin levels upon detachment may be reduced during leaf maturation. Thus, multiple developmental and environmental signals may converge to control auxin accumulation, which affects the efficiency of the WOX11/12-mediated DNRR from leaf explants.展开更多
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB27030103).
文摘De novo organ regeneration is the process in which adventitious roots or shoots regenerate from detached or wounded organs.De novo organ regeneration can occur either in natural conditions,e.g.adventitious root regenera-tion from the wounded sites of detached leaves or stems,or in in-vitro tissue culture,e.g.organ regeneration from cal-lus.In this review,we summarize recent advances in research on the molecular mechanism of de novo organ regen-eration,focusing on the role of the WUSCHEL-RELATED HOMEOBOX11(WOX11)gene in the model plant Arabidopsis thaliana.WOX11 is a direct target of the auxin signaling pathway,and it is expressed in,and regulates the establish-ment of,the founder cell during de novo root regeneration and callus formation.WOX11 activates the expression of its target genes to initiate root and callus primordia.Therefore,WOX11 links upstream auxin signaling to downstream cell fate transition during regeneration.We also discuss the role of WOX11 in diverse species and its evolution in plants.
基金supported by grants from the National Natural Science Foundation of China (31630007, 31770399 and 21375066)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB27030103)+1 种基金the Key Research Program of CAS (QYZDB-SSWSMC010)the National Key Laboratory of Plant Molecular Genetics
文摘De novo root regeneration(DNRR) has wide applications in agriculture such as those related to cutting technology. Detached Arabidopsis thaliana leaf explants can regenerate adventitious roots without added hormones. The regenerative ability is highly dependent on the developmental status of the leaf. An immature leaf has a higher regenerative ability, while a mature leaf is difficult to regenerate. Using RNASeq analysis, we showed that the expression levels of many genes, including those in the auxin network,changed during leaf maturation. Particularly, the expression levels of many YUCCA(YUC) genes in the auxin biosynthesis pathway are responsive to leaf maturation. Overexpression of YUC1 in the yuc-1 D dominant mutant rescued the rooting defects caused by leaf maturation. In addition, YUC4 expression levels were also affected by circadian rhythms. The regenerative ability was reduced in both immature and mature mutant leaf explants from the new wuschel-related homeobox 11-3(wox11-3) and wox12-3 mutant alleles created by the CRISPR/Cas9 method. Overall, the transcriptome and genetic data, together with the auxin concentration analysis, indicate that the ability to upregulate auxin levels upon detachment may be reduced during leaf maturation. Thus, multiple developmental and environmental signals may converge to control auxin accumulation, which affects the efficiency of the WOX11/12-mediated DNRR from leaf explants.
