Chrysanthemum is frequently attacked by aphids,which greatly hinders the growth and ornamental value of this plant species.WRKY transcription factors play an important role in the response to biotic stresses such as p...Chrysanthemum is frequently attacked by aphids,which greatly hinders the growth and ornamental value of this plant species.WRKY transcription factors play an important role in the response to biotic stresses such as pathogen and insect stresses.Here,chrysanthemum CmWRKY53 was cloned,and its expression was induced by aphid infestation.To verify the role of CmWRKY53 in resistance to aphids,CmWRKY53 transgenic chrysanthemum was generated.CmWRKY53 was found to mediate the susceptibility of chrysanthemum to aphids.The expression levels of secondary metabolite biosynthesis genes,such as peroxidase-and polyphenol oxidase-encoding genes,decreased in CmWRKY53-overexpressing(CmWRKY53-Oe)plants but dramatically increased in chimeric dominant repressor(CmWRKY53-SRDX)plants,suggesting that CmWRKY53 contributes to the susceptibility of chrysanthemum to aphids,possibly due to its role in the regulation of secondary metabolites.展开更多
Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant...Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant response to stress in Arabidopsis.However,how HDA9 targets to specific chromatin loci and controls gene expression netw orks involved in plant response to stress remains largely unclear.Here,we show that HDA9 represses stress tolerance response by interacting with and regulating the DNA binding and transcriptional activity of WRKY53,which functions as a high-hierarchy positive regulator of stress response.We found that WRKY53 is post-translationally modified by lysine acetylation at multiple sites,some of which are removed by HDA9,resulting in inhibition of WRKY53 transcription activity.Conversely,WRKY53 negatively regulates HDA9 histone deacetylase activity.Collectively,our results indicate that HDA9 and WRK53 are reciprocal negative regulators of each other's activities,illustrating how the functional interplay between a chromatin regulator and a transcription factor regulates stress tolerance in plants.展开更多
基金supported by the National Natural Science Foundation of China(31672192)the Agricultural Science and Technology Innovation Fund Project of Jiangsu Province(CX(18)2020)+1 种基金the National Key Research and Development Project(2018YFD1000402)the Foundation of Key Laboratory of Landscaping(KF201801).
文摘Chrysanthemum is frequently attacked by aphids,which greatly hinders the growth and ornamental value of this plant species.WRKY transcription factors play an important role in the response to biotic stresses such as pathogen and insect stresses.Here,chrysanthemum CmWRKY53 was cloned,and its expression was induced by aphid infestation.To verify the role of CmWRKY53 in resistance to aphids,CmWRKY53 transgenic chrysanthemum was generated.CmWRKY53 was found to mediate the susceptibility of chrysanthemum to aphids.The expression levels of secondary metabolite biosynthesis genes,such as peroxidase-and polyphenol oxidase-encoding genes,decreased in CmWRKY53-overexpressing(CmWRKY53-Oe)plants but dramatically increased in chimeric dominant repressor(CmWRKY53-SRDX)plants,suggesting that CmWRKY53 contributes to the susceptibility of chrysanthemum to aphids,possibly due to its role in the regulation of secondary metabolites.
基金This study was supported by the Discipline Innovation Team Foundation of Jianghan University(03100074)Natural Science Foundation of Hubei Province(grant no.2016CFB630)+1 种基金National Natural Science Foundation of China(NSFC31701100,NSFC31600981,and NSFC31600801)French ANR-19-CE12-0027-01.
文摘Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant response to stress in Arabidopsis.However,how HDA9 targets to specific chromatin loci and controls gene expression netw orks involved in plant response to stress remains largely unclear.Here,we show that HDA9 represses stress tolerance response by interacting with and regulating the DNA binding and transcriptional activity of WRKY53,which functions as a high-hierarchy positive regulator of stress response.We found that WRKY53 is post-translationally modified by lysine acetylation at multiple sites,some of which are removed by HDA9,resulting in inhibition of WRKY53 transcription activity.Conversely,WRKY53 negatively regulates HDA9 histone deacetylase activity.Collectively,our results indicate that HDA9 and WRK53 are reciprocal negative regulators of each other's activities,illustrating how the functional interplay between a chromatin regulator and a transcription factor regulates stress tolerance in plants.
