Network pharmacology to decipher the mechanism of Danggui Longhui Wan against chronic myeloid leukemia

Chronic myeloid leukemia(CML)is a hematopoietic myeloproliferative disorder.The Chinese prescription Danggui Longhui Wan(DGLHW)has been utilized in CML treatment,but its underlying mechanisms remain unclear.In this study,we gathered 794 constituents,1249 drug targets,1654 disease genes and 129 intersection genes.GO and KEGG were used to analyze the function of these genes.Compatibility of prescription study showed that monarch drug,minister drug,assistant and guide drug played a synergistic role in the treatment of CML.In addition,we obtained 20 hub genes and 12 key components.Molecular docking indicated that the main compounds and core proteins had good binding ability.The results of this study also showed that DGLHW might play a role in the treatment of CML by affecting MAPK,PI3K/AKT,FoxO and p53 signaling pathways.

Network pharmacology and molecular docking analysis reveal insights into the molecular mechanism of Gualou Qumai Wan in clear cell renal cell carcinoma

Background:To initially clarify the potential therapeutic targets and pharmacological mechanism regarding Gualou Qumai Wan(GQW),a kind of traditional Chinese medicine(TCM),in clear cell renal cell carcinoma(ccRCC)by virtue of the network pharmacology analysis and molecular docking analysis.Methods:The screening of bioactive components and targets of GQW was based on the Traditional Chinese Medicine System Pharmacology(TCMSP)and the UniProt platform served for standardizing their targets.Online Mendelian Inheritance in Man(OMIM),PharmGkb,TTD,DrugBank and GeneCards databases were searched to collect the disease targets of ccRCC.Cytoscape assisted in constructing herb-compound-target(H-C-T)networks.The STRING database was searched for constructing the target protein-protein interaction(PPI)networks,while the R programming language served for analyzing GO functional terms and the KEGG pathways related to potential targets.Analyses of core genes related to survival and tumor microenvironment(TME)were conducted respectively based on the GEPIA2 database and TIMER 2.0 database.Human Protein Atlas(HPA)and The Cancer Genome Atlas(TCGA)helped to obtain core genes’protein expression as well as transcriptome expression level.Autodock Vina software validated the molecular docking regarding GQW components and pivotal targets.Results:The constructed H-C-T networks mainly had 33 compounds and 65 targets.A topological analysis of the PPI network identified that ESR1,AKT1,HIF1A,PTGS2,TP53 and VEGFA serve as core targets in the way GQW affects ccRCC.According to the GO and KEGG pathway enrichment analyses,the effects of GQW are mediated by genes related to hypoxia and oxidative stress as well as the Chemical carcinogenesis-receptor activation and PI3K-Akt signaling pathways.AKT1 shows a close relation to the recruitment of various immune cells and can remarkably affect disease prognosis according to reports.Molecular docking and molecular dynamics simulations showed that diosgenin has higher affinity with core targets.Conclusion:The study makes a comprehensive explanation of the biological activity,potential targets,as well as molecular mechanism regarding GQW against ccRCC,which promisingly assists in revealing the action mechanism of TCM formulae in disease treatment and the respective and scientific basis.

面向运营商的SD-WAN演进思考

SD-WAN(Software Defined Wide Area Network,软件定义广域网)自2012年问世以来迅速发展。2019年8月,MEF发布了MEF70,推出了SD-WAN的第一个标准化定义。SDWAN凭借敏捷性、安全性、灵活性、多样性等特点在全球备受推崇,尤其是在疫情的影响下,众多企业加速数字化转型,SD-WAN服务将成为产业数字化时代融合通信的必然发展趋势。

Zuo Gui Wan Promotes Osteogenesis via PI3K/AKT Signaling Pathway:Network Pharmacology Analysis and Experimental Validation

Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW regulates osteogenesis is still unclear.The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.Methods A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells(BMSCs).Results In total,487 no-repeat targets corresponding to the bioactive components of ZGW were screened,and 175 target genes in the intersection of ZGW and osteogenesis were obtained.And 28 core target genes were then obtained from a PPI network analysis.A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress,metal ions,and lipopolysaccharide.Additionally,KEGG pathway enrichment analysis revealed that multiple signaling pathways,including the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway,were associated with ZGW-promoted osteogensis.Further experimental validation showed that ZGW could increase alkaline phosphatase(ALP)activity as well as the mRNA and protein levels of ALP,osteocalcin(OCN),and runt related transcription factor 2(Runx 2).What’s more,Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT,and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002.Finally,the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.Conclusion ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.

基于LoRaWAN的机场跑道警戒灯运行状态监视系统的设计与应用

随着物联网技术的快速发展,为解决无法及时监控反馈跑道警戒灯工作状态的问题,切实提高机场的运行安全裕度,完善跑道安全管理,文章在运用LoRaWAN技术手段实时监控远端警示灯工作状态的同时,通过统筹考虑软硬件设计提出充分利用无线广域网技术的解决方案。

Preparation of Dashanzha Wan by three-dimensional printing

Objective:To use three-dimensional(3D)printing technology to prepare Dashanzha Wan.Methods:The standard formula proportion of Dashanzha Wan was used to prepare printable materials(normally called the ink)for 3D printing,and different doses and shapes of Dashanzha Wan were prepared.Then,the rheological properties,texture characteristics,scanning electron microscopy,and content of ursolic acid were evaluated.Results:Dashanzha Wan ink showed good shear thinning properties,which is very suitable for 3D printing.The printed sample had a beautiful and regular shape with high resolution.Meanwhile,ursolic acid content in 3D-printed Dashanzha Wan aligned with the ursolic acid content shown in the Pharmacopoeia of the People's Republic of China 2020.Conclusion:The 3D-printed Dashanzha Wan has a better texture,and can be shaped into various shapes according to individual needs,which would increase patients’interest when taking medicine.Moreover,3D printing of Dashanzha Wan could be easily integrated into the digital life system,enabling online customization or use at home.This study reveals that 3D printing technology is a promising method for the production of traditional Chinese medicine with personalized appearance,dosage,and texture,which is suitable for a broader population.

Simiao Wan alleviates obesity-associated insulin resistance via PKCε/IRS-1/PI3K/Akt signaling pathway based on network pharmacology analysis and experimental validation

Background:The purpose of the study was to investigatethe active ingredients and potential biochemicalmechanisms of Simiao Wan(SMW)in obesity-associated insulin resistance.Methods:An integrated network pharmacology method to screen the active compoundsand candidate targets,construct the protein-protein-interaction network,and ingredients-targets-pathways network was constructed for topological analysis to identify core targets and main ingredients.To find the possible signaling pathways,enrichment analysis was performed.Further,a model of insulin resistance in HL-7702 cells was established to verify the impact of SMW and the regulatory processes.Results:An overall of 63 active components and 151 candidate targets were obtained,in which flavonoids were the main ingredients.Enrichment analysis indicated that the PI3K-Akt signaling pathway was the potential pathway regulated by SMW in obesity-associated insulin resistance treatment.The result showed that SMW could significantly ameliorate insulin sensitivity,increase glucose synthesis and glucose utilization and reduce intracellular lipids accumulation in hepatocytes.Also,SMW inhibited diacylglycerols accumulation-induced PKCεactivity and decreased its translocation to the membrane.Conclusion:SMW ameliorated obesity-associated insulin resistance through PKCε/IRS-1/PI3K/Akt signaling axis in hepatocytes,providing a new strategy for metabolic disease treatment.

The Governing Philosophy,Institutional Building and Policy Choice of the Rwanda Patriotic Front

The sound economic and governance performance in Rwanda has attracted wide attention in the international community.A lot of research into its specific policies has been done to look at the reasons behind the success of Rwanda.From the perspective of party politics,its success can be equated with that of the governance model of a party-centered country.

Classification of Villages in Wan’an County under the Background of Rural Revitalization

Rural revitalization strategy is an important strategic layout of rural development in the new era of the CPC Central Committee,and scientific and reasonable determination of village types is an important measure to promote rural revitalization by classification.The villages of Wan’an County were selected as the research object.Firstly,the factors that have a key impact on the development of the villages were extracted and qualitatively analyzed to preliminarily screen and judge their types.Secondly,the evaluation index system of development potential of the villages was constructed to quantitatively evaluate and reasonably classify the villages.Finally,the spatial distribution characteristics and quantity of different villages were revealed,and differentiated development strategies were proposed.

The Problems and Measures for Small Tourism Town Development in China:A Case Study of Wan Town

With the rapid development of tourism and urbanization in China,small tourism towns gradually grow toward prosperity.This paper takes Wan Town as an example to analyze the problem and countermeasures concerning the development of small tourism towns in China.There are a series of issues in the process of development of Wan Town,which includes uncoordinated architectural style,inaccurate market positioning,repeated cultural themes,and vulnerable linkage of the industrial chain.Based on the factor analysis,these development issues were classified into three categories:confusing architectural style,repetitive cultural theme,and weak linkage of industrial chain.To achieve the goal of sustainable development of small tourism towns,some countermeasures must be taken which include promoting the development of small tourism towns with the new rural revitalization,strengthening services improvement in small tourism towns,gathering industrial elements related to the tourism industry,and increasing tourism cultural connotation.

基于WAN环境的一种数据传输方式的实现

文中在一种基于ＷＷＷ的分布式信息系统的背景下，提出一种在广域网（ＷＡＮ）的环境中安全传输数据的解决方案。利用ＰＢ的数据管理技术，实现了方便、灵活的数据传输。数据传输功能是从分布的各节点的数据库中向总数据库传输数据，为系统提供数据基础。

基于意图的网络专利技术现状

2006年SDN的概念在学术界诞生,工业界即迅速跟进,很快发布了开源SDN控制器。至2016年,SDN不仅在理论上蓬勃发展,还进一步在白盒交换机、网络操作系统、数据中心网络虚拟化和SD-WAN四个领域培育出成熟的细分市场,已成为学术界与工业界协同发展的典范。

高效灵活的双WAN口路由器应用详解

双WAN口路由器技术已成熟,合理应用好双WAN口路由器能构建高效灵活的网络。在对多款该类型路由器进行了真实网络环境下的调试后,达到了稳定运行的目标,并以Cisco公司生产的一款企业级路由器为蓝本,详细介绍了该类型路由器与一般路由器相比所独具的五大功能的实现方法,具有高速、安全、稳定的特点,为广大中小企业提供了低成本的网络接入方案。

Effects of traditional Chinese medicine on symptoms of patients with spleen and stomach deficiency-related tinnitus

BACKGROUND Tinnitus affects 10%-30%of the population.Recent evidence suggests that tinnitus is associated with spleen deficiency.However,compared with kidney deficiency-related tinnitus,less research has been conducted on the impact of spleen defi-ciency-related tinnitus.AIM To investigate the clinical efficacy of using Guipi Wan and dietary and lifestyle modification based on traditional Chinese medicine for treatment of patients with spleen and stomach deficiency-related tinnitus.METHODS We enrolled 110 patients with spleen and stomach deficiency-related tinnitus who were distributed into treatment(58 cases)and control(52 cases)groups.Tinnitus severity,sleep quality and emotional state were assessed by questionnaires[Tinnitus Evaluation Questionnaire(TEQ);Pittsburgh Sleep Quality Index(PSQI);Depression-Anxiety-Stress Scale-21(DASS-21)]that were used for analysis in the two groups during the initial and intervention and after.In the treatment group,patients were treated with oral administration of Guipi Wan and consulted for healthy dietary and lifestyle modification.In the control group,patients were only assessed and not treated.RESULTS At the end of the 6-months,TEQ scores decreased significantly in the treatment group(P=0.021)but not in the Control group.Significant effects in the treatment group were noted for PSQI total score(P=0.043)and several PSQI component scores in the treatment group(P<0.05).After treatment,the DASS-21 scores were significantly reduced in the treatment group(P<0.05).CONCLUSION Guipi Wan combined with dietary and lifestyle modification based on regulating the spleen and stomach can be considered core to the treatment of tinnitus related to spleen and stomach deficiency.

SD-WAN服务持续升级 带来云网络新业态

众所周知,SD-WAN于2015年前后出现,到2018年开始规模商用,经过这几年的发展,已经成为企业组网市场上最热门的业务之一。在海外市场,由于MPLS VPN等专线类业务与SD-WAN差价很大,而且企业上云已经达到了一定规模(企业上云比例超过80%),因此近几年SD-WAN的业务已经从增量市场走向了存量专线替代;而在国内市场,由于企业上云还处在快速增长期,新增的组网需求较为强烈,从我国企业上云比例在40%~50%之间的现状来看,目前SD-WAN的增长主要还是增量的连接需求。

Formation and syn-rifting process of the Wan'an Basin, South China Sea

Based on seismic and drilling data, we calculated tectonic subsidence amounts and rates of the Wan'an Basin by backstripping. The genetic mechanism and syn-rifting process of the basin were analyzed in combination with the regional geological setting. The results reveal that the basin syn-rifted in the Eocene and early Miocene under the control of the dextral strike-slip Wan'an Fault Zone. The transtensional/ extentional stresses along this fault zone may be attributed to seafloor spreading of the South China Sea (SCS) in multiple episodes. Extensive basal faults and some small initial rifts in the early Paleogene can be related to southeastward extrusion and clockwise rotation of the Indochina Block. During the Oligocene, the nearly N-S directed spreading of the SCS derived the transtensional stresses in a roughly NW-SE orientation. The basin subsided rapidly in the middle and north to form two major subsidence centers. In the early Miocene, the SCS spread again in a nearly NW-SE direction, resulting in rapid subsidence in the southern basin continuous extending until the period ～16.3 Ma.

FreeS/WAN IPSec安全技术在数字图书馆虚拟专用网控制信息访问中的实现

随着现代图书馆物理馆舍和虚拟馆藏不断扩张,信息安全问题日显突出。Internet协议(IP)是数字图书馆网络赖以构建的基础。然而,IP本身却并不明安全的。因此,要解决数字图书馆的安全问题,必须首先解决的IP的安全问题。本文拟采用VPN解决方案,引入FreeS/WAN IPSec来解决数字图书馆信息访问中的安全问题。

WAN路由器备份链路的设计与实现

阐述了广域网链路之一—— DDR的工作原理 ,提出了一种设计思路并且加以实现 ,并说明了配置文件、拨号过程跟踪和链路速率检测的工作过程。该方法具有较好的实用性 。

对Squire和Wan Der Tak多目标投资决策模型的一点改进

一、投资项目决策的单一目标和多目标:问题的提出投资是将货币资金转化为固定资产,再由固定资产的运营而获取收益的过程,它是实现经济增长的关键。根据国际国内通行的做法,投资项目在决定是否上马之前必须进行项目分析,或者项目评估,以便为投资决策提供依据。所以,项目评估又可以称为决策支持。投资项目决策的目标是多元的。从投资者的角度来说。

Frees/wan在Linux下的安装

基于3DES等加密算法的Frees/wan从应用情况看,是目前最安全的在Linux上构建VPN网络的实现方案。结合实际,介绍了在Linux系统上安装Frees/wan的过程。