Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Meth...Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.展开更多
OBJECTIVE:To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases(MMPs)/tissue inhibitor metalloproteinases(TIMPs) secreted by cult...OBJECTIVE:To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases(MMPs)/tissue inhibitor metalloproteinases(TIMPs) secreted by cultured endometrial cells from patients with endometriosis.METHODS:Ectopic and eutopic endometrial cells obtaind from 15 endometriosis patients were cultured in vitro,and divided randomly into five groups:high dose;moderate dose;low dose;nemestran;blank control.The three dose groups were treated with a decoction prepared according to the principle of warming Yang and removing blood stasis;nemestran and 0.9%NaCI were administered to the nemestran group and balnk control group,respectively.Eutopic endometrial cells obtaind from 10 hysteromyoma patients were cultured in vitro,as the normal control group,0.9%NaCI were administered to the normal control group.Cell culture supernatants were collected and levels of matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),tissue inhibitor metalloproteinase-1(TIMP-1) and tissue inhibitor metalloproteinase-2(TIMP-2) detected by enzyme-linked immuno sorbent assay(ELISA).RESULTS:Compared with the normal control group,levels of MMP-1,MMP-2,and MMP-9 in eutopic and ectopic endometrium cell supernatants in the blank control group were increased,whereas levels of TIMP-1 and TIMP-2 were decreased(P <0.05).Compared with the blank control group,levels of MMP-1 and MMP-2 in ectopic and eutopic endometrium cell supernatants cultured in low-dose,middle-dose,and high-dose groups were decreased,whereas levels of TIMP-1 and TIMP-2 were increased significantly(P < 0.05).CONCLUSION:The warming Yang and removing blood stasis method affects expression of MMPs andTIMPs.展开更多
目的观察温针灸治疗阳气亏虚血瘀型慢性心力衰竭的临床疗效及对患者心功能和生活质量的影响。方法将60例阳气亏虚血瘀型慢性心力衰竭患者用随机数字表法分为对照组(30例)和研究组(30例)。两组均予内科常规基础治疗,对照组另予常规针刺治...目的观察温针灸治疗阳气亏虚血瘀型慢性心力衰竭的临床疗效及对患者心功能和生活质量的影响。方法将60例阳气亏虚血瘀型慢性心力衰竭患者用随机数字表法分为对照组(30例)和研究组(30例)。两组均予内科常规基础治疗,对照组另予常规针刺治疗,研究组另予温针灸治疗。观察两组治疗前后明尼苏达心功能不全生命质量量表(Minnesota living with heart failure questionnaire,MLHFQ)评分、6分钟步行试验(6-minute walk test,6MWT)结果、心功能指标[左室舒张末期内径(left ventricular end diastolic diameter,LVEDD)、左心室收缩末内径(left ventricular end systolic diameter,LVESD)和左室射血分数(left ventricular ejection fraction,LVEF)]以及血清肾素、醛固酮(aldosterone,ALD)、血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)和血浆N末端B型利钠肽原(N-terminal pro-B-type natriuretic peptide,NT-proBNP)水平的变化,比较两组临床疗效。结果两组治疗后LVEDD、LVESD、肾素水平、ALD水平、AngⅡ水平、NT-proBNP水平和MLHFQ评分均较治疗前降低(P<0.05),LVEF和6MWT水平均较治疗前提高(P<0.05)。研究组治疗后LVEDD、LVESD、肾素水平、ALD水平、AngⅡ水平、NT-proBNP水平和MLHFQ评分均低于对照组(P<0.05),LVEF和6MWT水平均高于对照组(P<0.05)。研究组总有效率高于对照组(P<0.05)。结论在内科常规治疗基础上,温针灸治疗阳气亏虚血瘀型慢性心力衰竭的临床疗效优于常规针刺,可改善心脏功能,提高生活质量。展开更多
基金Key R&D Project of Liaoning Provincial Science and Technology Department(No.2018225042)
文摘Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.
基金Supported by the Innovation Fundation of Chinese Medicine research,Guangzhou University of Chinese Medicine!Effect of Chinese Medicines in the Warming Yang and Removing Blood Stasis Method on the Biological Characteristics of Cultured Endometrial Cells from Patients with Endometriosis,No.K0080053)the Natural Science Foundation of Heibei Province of China(Effect of Dang Gui Si Ni Jia Wu Zhu Yu Sheng Jiang Huang Jiu Decoction via NF-KBp65signaling pathway on the regulating endometrial Biological Characteristics of endometriosis.No.2015CFB590)
文摘OBJECTIVE:To investigate the effect of Chinese medicines using the warming Yang and removing blood stasis method on levels of matrix metalloproteinases(MMPs)/tissue inhibitor metalloproteinases(TIMPs) secreted by cultured endometrial cells from patients with endometriosis.METHODS:Ectopic and eutopic endometrial cells obtaind from 15 endometriosis patients were cultured in vitro,and divided randomly into five groups:high dose;moderate dose;low dose;nemestran;blank control.The three dose groups were treated with a decoction prepared according to the principle of warming Yang and removing blood stasis;nemestran and 0.9%NaCI were administered to the nemestran group and balnk control group,respectively.Eutopic endometrial cells obtaind from 10 hysteromyoma patients were cultured in vitro,as the normal control group,0.9%NaCI were administered to the normal control group.Cell culture supernatants were collected and levels of matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),tissue inhibitor metalloproteinase-1(TIMP-1) and tissue inhibitor metalloproteinase-2(TIMP-2) detected by enzyme-linked immuno sorbent assay(ELISA).RESULTS:Compared with the normal control group,levels of MMP-1,MMP-2,and MMP-9 in eutopic and ectopic endometrium cell supernatants in the blank control group were increased,whereas levels of TIMP-1 and TIMP-2 were decreased(P <0.05).Compared with the blank control group,levels of MMP-1 and MMP-2 in ectopic and eutopic endometrium cell supernatants cultured in low-dose,middle-dose,and high-dose groups were decreased,whereas levels of TIMP-1 and TIMP-2 were increased significantly(P < 0.05).CONCLUSION:The warming Yang and removing blood stasis method affects expression of MMPs andTIMPs.
文摘目的观察温针灸治疗阳气亏虚血瘀型慢性心力衰竭的临床疗效及对患者心功能和生活质量的影响。方法将60例阳气亏虚血瘀型慢性心力衰竭患者用随机数字表法分为对照组(30例)和研究组(30例)。两组均予内科常规基础治疗,对照组另予常规针刺治疗,研究组另予温针灸治疗。观察两组治疗前后明尼苏达心功能不全生命质量量表(Minnesota living with heart failure questionnaire,MLHFQ)评分、6分钟步行试验(6-minute walk test,6MWT)结果、心功能指标[左室舒张末期内径(left ventricular end diastolic diameter,LVEDD)、左心室收缩末内径(left ventricular end systolic diameter,LVESD)和左室射血分数(left ventricular ejection fraction,LVEF)]以及血清肾素、醛固酮(aldosterone,ALD)、血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)和血浆N末端B型利钠肽原(N-terminal pro-B-type natriuretic peptide,NT-proBNP)水平的变化,比较两组临床疗效。结果两组治疗后LVEDD、LVESD、肾素水平、ALD水平、AngⅡ水平、NT-proBNP水平和MLHFQ评分均较治疗前降低(P<0.05),LVEF和6MWT水平均较治疗前提高(P<0.05)。研究组治疗后LVEDD、LVESD、肾素水平、ALD水平、AngⅡ水平、NT-proBNP水平和MLHFQ评分均低于对照组(P<0.05),LVEF和6MWT水平均高于对照组(P<0.05)。研究组总有效率高于对照组(P<0.05)。结论在内科常规治疗基础上,温针灸治疗阳气亏虚血瘀型慢性心力衰竭的临床疗效优于常规针刺,可改善心脏功能,提高生活质量。
