The measurement of 23 organochlorine, organophosphorus, and pyrethroid pesticides in typical traditional Chinese medicine (TCM), flos lonicerae, was made using gel permeation chromatography (GPC) purification and gas ...The measurement of 23 organochlorine, organophosphorus, and pyrethroid pesticides in typical traditional Chinese medicine (TCM), flos lonicerae, was made using gel permeation chromatography (GPC) purification and gas chroma- tography-mass spectrometry (GC-MS) detection. The pesticides were extracted with ultrasonic device and 5.0 mL mixture of ethyl acetate and cyclohexane (1:1, v/v). Coextractants from sample matrices which may have interfere to the qualitative and quantitative analysis, such as pigments, were removed using GPC purification. Simultaneous full scan and selective ion monitor (scan/SIM) mode for GC-MS was used for qualitative and quantitative analysis, which pro- vided retention time and characteristic fragments ratio for each pesticide so as to positively identify each analyte. Rela- tive standard deviations (RSDs) were within 7.7% (5.0 - 22.5 μg/kg, n = 3). The recoveries of pesticide standards at the spiked concentration of 5.0 - 22.5 μg/kg were between 87.1% and 110.9%. Limits of detection (LODs) for the analytes were 0.16 - 3.2 μg/kg, which could meet the demand of routine analysis and TCM quality control.展开更多
The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis f...The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.展开更多
目的系统评价不同中医外治法干预卒中后神经源性膀胱(PSNB)的临床效果。方法计算机检索中国知网、万方数据知识服务平台、维普资讯中文期刊服务平台、中国生物医学文献服务系统、Web of Science、The Cochrane Library、Embase、PubMed...目的系统评价不同中医外治法干预卒中后神经源性膀胱(PSNB)的临床效果。方法计算机检索中国知网、万方数据知识服务平台、维普资讯中文期刊服务平台、中国生物医学文献服务系统、Web of Science、The Cochrane Library、Embase、PubMed等数据库,搜集公开发表的采用中医外治法干预PSNB的随机对照试验。经筛选文献、质量评价、提取资料后,使用STATA 17.0软件进行网状Meta分析。结果最终纳入35篇文献,共涉及9种中医外治法。网状Meta分析结果显示,与常规治疗相比,艾灸+常规治疗、针刺+常规治疗、穴位贴敷+常规治疗、刮痧+常规治疗、艾灸+针刺+常规治疗、艾灸+耳穴疗法+常规治疗、艾灸+穴位贴敷+常规治疗的总有效率更高,耳穴疗法+常规治疗、艾灸+常规治疗、针刺+常规治疗、艾灸+针刺+常规治疗、艾灸+耳穴疗法+常规治疗、艾灸+穴位贴敷+常规治疗在改善PSNB患者残余尿量上效果更好,耳穴疗法+常规治疗、艾灸+常规治疗、针刺+常规治疗、艾灸+针刺+常规治疗、艾灸+穴位贴敷+常规治疗在改善PSNB患者膀胱安全容量上效果更好(P<0.05)。其中,艾灸+穴位贴敷+常规治疗在提高总有效率、改善膀胱安全容量方面效果最佳,艾灸+耳穴疗法+常规治疗在改善残余尿量方面效果最佳。结论与常规治疗相比,采用不同中医外治法联合常规治疗干预PSNB可获得更好的治疗效果,更有效地减少残余尿量、增加膀胱安全容量,从而改善患者排尿情况,提高其生活质量。其中,在常规治疗基础上,艾灸+穴位贴敷在提高有效率、增加膀胱安全容量方面效果最佳,艾灸+耳穴疗法在改善残余尿量指标方面效果最佳。展开更多
文摘The measurement of 23 organochlorine, organophosphorus, and pyrethroid pesticides in typical traditional Chinese medicine (TCM), flos lonicerae, was made using gel permeation chromatography (GPC) purification and gas chroma- tography-mass spectrometry (GC-MS) detection. The pesticides were extracted with ultrasonic device and 5.0 mL mixture of ethyl acetate and cyclohexane (1:1, v/v). Coextractants from sample matrices which may have interfere to the qualitative and quantitative analysis, such as pigments, were removed using GPC purification. Simultaneous full scan and selective ion monitor (scan/SIM) mode for GC-MS was used for qualitative and quantitative analysis, which pro- vided retention time and characteristic fragments ratio for each pesticide so as to positively identify each analyte. Rela- tive standard deviations (RSDs) were within 7.7% (5.0 - 22.5 μg/kg, n = 3). The recoveries of pesticide standards at the spiked concentration of 5.0 - 22.5 μg/kg were between 87.1% and 110.9%. Limits of detection (LODs) for the analytes were 0.16 - 3.2 μg/kg, which could meet the demand of routine analysis and TCM quality control.
基金the National Natural Science Foundation of China (Grant No. 21175041)the National Basic Research Program (Grant No. 2012CB910602)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) for financial supports
文摘The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents.
