Photocatalytic Degradation of Water-Soluble Dyes by LaCoO_3

Pervoskite type oxides LaCoO 3 was prepared by citrate method with the granula of 20 nm-30 nm. With a fluorescent Hg lamp or sunlight as irradiator, the degradation experiments of various water soluble dyes were carried out in the suspension system of LaCoO 3 . The results show that the pervoskite type oxide LaCoO 3 has good photocatalytic activity.Studied by X ray photoelectron spectroscopy and photoacoustic spectra, its photocatalytic activity is found to be related with factors such as the d electron structure of ion Co 3+ ,Co—O binding energy and adsorbed oxygen on the surface etc.

Anti-tumor Effect and Protective Effect on Chemotherapeutic Damage of Water Soluble Extracts from Hedyotis diffusa

Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.

Photocatalytic Degradation of Water-Soluble Dyes by LaCoO3

Pervoskite type oxides LaCoO 3 was prepared by citrate method with the granula of 20 nm-30 nm. With a fluorescent Hg lamp or sunlight as irradiator, the degradation experiments of various water soluble dyes were carried out in the suspension system of LaCoO 3 . The results show that the pervoskite type oxide LaCoO 3 has good photocatalytic activity.Studied by X ray photoelectron spectroscopy and photoacoustic spectra, its photocatalytic activity is found to be related with factors such as the d electron structure of ion Co 3+ ,Co—O binding energy and adsorbed oxygen on the surface etc.

Photocatalytic Degradation of Water-Soluble Dyes by LaCoO_3

Perovskite type oxides LaCoO 3 was prepared by citrate method in granula of 20～30 nm. Using a fluorescent Hg lamp or sunlight as irradiator, the degradation experiments of various water soluble dyes were carried out in the suspension system of LaCoO 3. The results show that the perovskite type oxide LaCoO 3 has good photocatalytic activity. With the study of X ray photoelectron spectroscopy and photoacoustic spectra, its photocatalytic activity is mainly related with the factors such as the d electron structure of ion Co 3+ , Co O binding energy and adsorbed oxygen on the surface etc.

Response of antioxidase in viscera of Pagrosuma Major larvae to water soluble fraction of hydrocarbons in No.0 diesel oil

Pagrosomus major larvae were exposed to the water soluble fraction of hydrocarbon in No.0 diesel oil (corresponding to No.2 fuel oil) at concentrations of 0, 0 17, 1.22 and 8 82 mg/L for up to 15 days. Larvae were sampled on days 9 and l5 of the experiment. Supernatants of viscera tissue extractions were assayed for biochemical response in terms of oxidative stress superoxide dismutase(SOD), activity of selenium dependant glutathione peroxidase(Se GPx) and catalase(Ca), and the concentration of reduced glutathione(GSH). On day 9 of exposure, statistically significant dose related increases in Se GPx and SOD activity, and GSH concentration were observed in all cases except for Se GPx activity under the highest dosage of hydrocarbon. However, on day l5 of exposure, a similar dose related response was only observed for Se GPx activity. GSH concentration decreased and SOD activity showed no statistical difference as compared to controls. However, a significant decrease in compared to day 9 Se GPx activity and GSH concentration, in contrast to increase SOD activity at day 15 as indicates an accelerated accumulation of H 2O 2 and potential oxidative damage under long term exposure of larvae to hydrocarbons. No statistical changes were observed in Ca activity throughout the experiment, possibly owing to the high efficiency of Se GPx. A recovery experiment was performed on indicating that the response of antioxidants measured tending to return to their control levels. These results prove the function of the antioxidant defense system of the larvae to the water soluble fraction of hydrocarbons in No.0 diesel oil.

Biodegradability of soil water soluble organic carbon extracted from seven different soils

Water soluble organic carbon （WSOC） is considered the most mobile and reactive soil carbon source and its characterization is an important issue for soil ecology study. A biodegradability test was set up to study WSOC extracted from 7 soils differently managed. WSOC was extracted from soil with water （soil/water ratio of 1：2, W/V） for 30 min, and then tested for biodegradability by a liquid state respirometric test. Result obtained confirmed the finding that WSOC biodegradability depended on the both land use and management practice. These results suggested the biodegradability test as suitable method to characterize WSOC, and provided useful information to soil fertility.

Study on the Rapid Method to Predict Longevity of Controlled Release Fertilizer Coated by Water Soluble Resin

The study discussed the rapid method to test and predict the longevity of controlled release fertilizers （CRFs） coated by water soluble resin by using the short-term leaching under higher temperature. Pure water dissolving incubation and higher temperature leaching were used to study the patterns of the nutrient release of the CRFs. The correlation analysis between the days at 25℃ and the hours at 80℃ of Trincote 1 and Trincote 2 for the same cumulative release rates were conducted. Patterns of cumulative nutrient release curve followed one factor quadratic regression equation at each given temperatures, and each of relative coefficient was bigger than 0.995. As the temperature increased, nutrients release of the CRFs increased. The longevity of resin coated CRFs were predicted by use of both the cumulative nutrients release equation at 80℃ and the regression equation of release time needed for the same cumulative release rates between 25 and 80℃. There were only 0.3-6.9% relative errors between the tested longevity and predicted one. In conclusion, the longevity of resin coated CRFs could be predicted more quickly and precisely by use of the higher temperature short-term leaching method than that of the traditional differential release rate. The longevity of resin coated CRF could be rapidly and precisely predicted in a few hours by application of the higher temperature shortterm leaching method.

Improvement of humidity resistance of water soluble core by precipitation method

Water soluble core has been widely used in manufacturing complex metal components with hollow configurations or internal channels;however,the soluble core can absorb water easily from the air at room temperature.To improve the humidity resistance of the water soluble core and optimize the process parameters applied in manufacturing of the water soluble core,a precipitation method and a two-level-three-full factorial central composite design were used,respectively.The properties of the cores treated by the precipitation method were compared with that without any treatment.Through a systematical study by means of both an environmental scanning electron microscope(ESEM) and an energy dispersive X-ray(EDX) analyzer,the results indicate that the hygroscopicity can be reduced by 20% and the obtained optimal process conditions for three critical control factors affecting the hygroscopicity are 0.2 g·mL-1 calcium chloride concentration,4% water concentration and 0 min ignition time.The porous surface coated by calcium chloride and the high humidity resistance products generated in the precipitation reaction between calcium chloride and potassium carbonate may contribute to the lower hygroscopicity.

PREPARATION AND PHOTOSENSITIVITY OF WATER SOLUBLE PHENOLIC RESINS CONTAINING ACRYLOYL AND QUATERNARY AMMONIUM CHLORIDE GROUPS

New water soluble and photocrosslinkable prepolymers containing acrylate and quaternary ammonium salt groups were synthesized from epoxy phenolic resin via ring-opening reaction with acrylic acid and with aqueous solution of triethylamine hydrochloride successively. The second reaction needs no phase transfer catalyst to accelerate, since the product formed can act as a phase transfer catalyst. The prepolymer obtained contains both photocrosslinkable acrylate groups and hydrophilic quaternary ammonium salt groups. Optimum conditions for these reactions were studied. The photosensitivity of the prepolymer was also investigated. The effects of different photoinitiators, different crosslinkable diluent monomers and amine accelerator on the photosensitivity of the prepolymer were compared. The photoinitiator of hydrogen abstraction type is still effective without using amine or alcohol as accelerator, because the prepolymer contains a H beside the OH groups formed in the ring-opening reactions.

Partitioning of water soluble organic carbon in three sediment size fractions:Effect of the humic substances

Water soluble organic carbon （WSOC） in sediments plays an important role in transference and transformation of aquatic pollutants. This article investigated the inherent mechanisms of how sediemnt grain size affect the partitioning coeffcient （k） of WSOC. Influences of NaOH extracted humic substances were particularly focused on. Sediments were sampled from two cross-sections of the middle Yellow River and sieved into three size fractions （〈 63 μm, 63-100 μm, and 100-300 μm）. The total concentration of WSOC in sediments （Cwsoc） and k were estimated using multiple water-sediment ratio experiments. Results showed that Cwsoc ranged from 0.012 to 0.022 mg/g, while k ranged from 0.8 to 3.9 L/kg. Correlations between the spectrum characteristics of NaOH extracted humic substances and k were analyzed. Strong positive correlations are determined between k and the aromaticity indicators of NaOH extracted humic substances in different sediment size fractions. Comparing with finer fractions （〈 63 μm）, k is higher in larger size fractions （63- 100 μm and 100-300 μm） related to higher aromaticity degree of NaOH extracted humic substances mostly. While negative relationship between k and the area ratio of fourier transform infrared spectroscopy （FT-IR） at 3400 and 1430 cm^-1 implied that the lowest k was related to the highest concentration of acidic humic groups in particles 〈 63 μm. WSOC in finer fractions （〈 63 μm） is likely to enter into pore water, which may further accelerate the transportation of aquatic contaminants from sediment to water.

Water-soluble lipopolymer delivery of N-methyl-D-aspartic acid receptor 2B siRNA relieves chronic neuropathic pain in rats

Spinal dorsal horn N-Methyl-D-aspartic acid receptor 2B （NR2B） overexpression plays an important role in the production and maintenance of neuropathic pain. Because small interfering RNA （siRNA） can inhibit NR2B expression, siRNA may provide a novel approach to treat neuropathic pain and possibly nerve injury. However, an efficient and safe vector for NR2B siRNA has not been discovered. This study shows that a water soluble lipopolymer （WSLP） comprised of low molecular weight polyethyleneimine （PEI） and cholesterol can deliver siRNA targeting NR2B for the treatment of neuropathic pain. Results show that intrathecal injection of WSLP/siRNA complexes for 3 days inhibit NR2B gene expression with reductions in mRNA and protein levels by 59% and 54%, respectively, compared with control rats （P 〈 0.01）. Injection of WSLP complexed with scrambled siRNA, or PEI with siRNA did not show this inhibitory effect. Moreover, injection of WSLP/siRNA complexes significantly relieved neuropathic pain at 3, 7, 12, and 21 days, while injection of WSLP with scrambled siRNA or PEI with siRNA did not. These results demonstrate that WSLP can efficiently deliver siRNA targeting NR2B in vivo and relieve neuropathic pain.

Fluorescence characteristics of water soluble organic carbon in eastern China

Fluorescence excitation and average molecular weight of 46 water soluble organic matter (WSOC) samples extracted from 20 soil types in eastern China were determined. It was found all samples shared similar spectroscopy. A good linear relationship existed between total organic carbon and excitation in the range of 350 to 450 nm though the content of organic carbon and pH of the samples vary in a wide range. No significant correlation between relative excitation intensity and average molecular weight of WSOC and FA was found, but the partial correlation became significant with pH as the controlling factor for WSOC samples. The relative excitation intensity showed a general trend of increasing from south to north in the study area. The pH value might play an important role in regulating the fluorescent spatial variation of WSOC. S153 A

Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil

To investigate the effect of the water soluble fraction of crude oil(WSF) on marine bivalves, the scallop C hlamys farreri was exposed to three WSF concentrations(0.18 mg/L, 0.32 mg/L, and 0.51 mg/L, respectively) in seawater. Petroleum hydrocarbon contents in scallops and a suite of enzymes [7-Ethoxyresorufin-O-deethylase(EROD), aryl hydrocarbon hydroxylase(AHH), glutathione S-transferase(GST), and glutathione peroxidase(GPx)] in gills and digestive glands were monitored over 10 days. The results revealed that WSF affected the activity of the four enzymes in the gills and digestive glands. EROD activity in the gills was significantly induced in most individuals of the three test groups, while in the digestive gland it was significantly induced in the low-concentration group within 4 days but was inhibited in the middle- and high-concentration groups on days 1, 4, and 10. AHH activity in the gills of all treatment groups was significantly induced on day 1. In the digestive gland, AHH activity was induced in most individuals from the treatment groups. In all treatment groups, GST activity was significantly inhibited from days 2 to 10 in the gills and was induced after day 4 in the digestive gland. GPx activity in the gills was significantly inhibited throughout the exposure period in all treatment groups. There was no overall significant difference in GPx activity in the digestive gland between the control and treatment groups. Our results also revealed that petroleum hydrocarbon concentrations in the tissues increased linearly with exposure time. EROD activity in the digestive gland and GST and GPx activity in the gill tissue were negatively correlated with petroleum hydrocarbon body burden. These enzymes play important roles in detoxification and can act as potential biomarkers for monitoring petroleum hydrocarbon contaminants in the marine environment.

The Preparation of 2-O-Acetonyl-β-cyclodextrin as One Key Intermediate for Water Soluble β-Cyclodextrin Derivatives

O-Acetonyl-b-cyclodextrin with a suitable average substitution degree of about 5.8, which could be used as a key intermediate for water soluble functional b-cyclodextrin derivatives, was prepared based on the proper oxidation of heptakis (6-O-dimethyl-tert-butylsilyl)-2-O -hydroxypropyl-b-cyclodextrin.

STRUCTURAL STUDIES OF AN ARABINOGALACTAN——A WATER SOLUBLE POLYSACCHARIDE FROM THE POLLEN OF PAPAVER SOMNIFERUM

A polysaccharide,PS(1),has been isolated from the pollen of papaver somniferum.PS(1)is a highly branched arabinogalactan,which,mainly contains α—L—arabinofuranosyl(1→)and(3,6→1)— β—D—galactopyranosyl residues.We also studied the circular dichroism and immunoactivity of PS(1).

WATER SOLUBLE DISPERSE DYES CONTAINING CARBOXY-METHYL-SULFONYL GROUP

Water soluble disperse dyes of either monoazo or anthraquinone types containing the carboxymethylsulfonylgroup（-SO2CH2COOH）were synthesized.Ammonium salts of this type of dyes are more soluble thanother salts.These dyes are suitable for pad dyeing or printing on polyester fiber and polyester/cottonblends in either weak acid,neutral or weak alkali medium without a dispersing agent.The formation of-SO2CH3 group from -SO2CH2COOH group after fixing on the fiber was proved.

The Fabrication of Water-Soluble Chitosan Capsule Shell Modified by Alginate and Gembili Starch(Dioscorea esculenta L)

Capsule shells have been successfully fabricated from water-soluble chitosan(WSC)with the addition of alginate and Gembili starch.WSC was synthesized from crab shell chitosan by depolymerization reaction.The capsule shells were made with the composition of WSC:Alginate,2:1,3:1 and 4:1(w/w)with and without the addition of Gembili starch.Gembili starch was added with a ratio of Alginate:Starch,1:1(w/w).The capsule shell properties were evaluated according to Indonesian Pharmacopoeia standard.The solubility test showed that the capsule shells were comply with the standard.The highest degrees of swelling in water and HCl 0.1 N solution were 491.93%and 410.51%,respectively.The highest degradation percentages in water and HCl 0.1 N solution were 57.80%and 21.44%,respectively.The observation of physical appearance indicated that the capsule shell with WSC:Alginate:Starch in ratio of 3:1:1 has appearance close to commercial capsule shell.

THE STUDY ON THE WATER SOLUBLE PEPTIDES IN PAPAVER SOMNIFERUM POLLEN EXTRACTION,PURIFICATION,SEQUENCE DETERMINATION AND SYNTHESIS

Four peptides PSPP1,PSPP2,PSPP3 and PSPP4 were purified from the water-extract of Papaver somniferum pollen.Their sequences,with 21,17,13 and 16 amino acid residues respectively,have been determined by Edman degradation-N-terminal dansylation.PSPP2, PSPP3 and PSPP4 were synthesized using solid phase method.The immunopromotive activities of PSPP1,PSPP2,PSPP3,PSPP4 and the initially separated sample PSPP have been also observed by the methods of counting erythrocyte rosette forming cells(ERFC) and T-lymphocyte transformation test in vitro.

CARBOXYMETHYLPACHYMAN(Ⅰ),A NEW WATER SOLUBLE POLYSACCHARIDE WITH MARKED ANTITUMOUR ACTIVITY

Carboxymethylpachyman(Ⅰ)was formed by carboxymethylation of β-pachyman.The antitumour activity of carboxymethylpachyman(Ⅰ)against S_(180)、EAC、 MA、U_(14)was measured.The structure of carboxymethylpachyman(Ⅰ)was proved by IR ^(13)CNMR spectroscopy.

SYNTHESIS AND PARTIAL CHARACTERIZATION OF WATER SOLUBLE RAPE POLLEN DODECAPEPTIDE

A dodecapeptide extracted from rape pollen was synthesized using solid—phase method,its conforma- tions in different states were studied by FT—IR,CD,and its physiological activities were determined.Seven segments and five analogs were also synthesized to elucidate the structure—function relation.