Photosynthetic Water Use Efficiency of Heritage and Modern Potatoes under Limited and Unlimited Water Environments

Photosynthetic capacity for heritage (Taewa) and modern potato cultivars were compared at different water and nitrogenregimes in the glasshouse and field. The glasshouse was 2*2*4 factorial design with two irrigation: 100% ET and 60% ET;two applied N: 50 kg N ha-1 and 200 kg N ha-1, two Taewa (Moe Moe, Tutaekuri) and two modern potatoes (Moonlight, Agria). The 2009/2010 field experiment was a split-plot, with irrigation and rain-fed regimes as the main treatments: four potatoes above were sub-treatments. The 2010/2011 field experiment was a split-split-plot, with three water regimes as the main treatments: three cultivars (Moe Moe, Tutaekuri, and Agria) were subplots;two N rates were sub-sub-treatments. Gaseous exchange was measured by CIRAS-2 at different days from emergence. Leaf water potential was measured using pressure chamber method. Taewa achieved high photosynthetic WUE in glasshouse and 2010/2011 experiment by maintaining high An, low gs and low Ci compared to modern cultivars (p The An, gs and T increased with irrigation and N increase while decreasing Ci (p < 0.01). Water stress significantly increased VPD resulting in low An and photosynthetic WUE in Moonlight in the glasshouse. The leaf water potential for Taewa was very tolerant while modern potatoes were weakened by water stress. The study indicated that Taewa can be scheduled at partial irrigation without more detrimental effects on photosynthetic capacity while modern potatoes need full irrigation to avoid detrimental effects on photosynthetic capacity.

Effects of thinning and understory removal on water use efficiency of Pinus massoniana:evidence from photosynthetic capacity and stable carbon isotope analyses

Understanding the relationship between forest management and water use efficiency(WUE)is important for evaluating forest adaptability to climate change.However,the effects of thinning and understory removal on WUE and its key controlling processes are not well understood,which limits our comprehension of the physiological mechanisms of various management practices.In this study,four forest management measures(no thinning:NT;understory removal:UR;light thinning:LT;and heavy thinning:HT)were carried out in Pinus massoniana plantations in a subtropical region of China.Photosynthetic capacity and needle stable carbon isotope composition(δ^(13)C)were measured to assess instantaneous water use efficiency(WUE_(inst))and long-term water use efficiency(WUE_(i)).Multiple regression models and structural equation modelling(SEM)identified the effects of soil properties and physiological performances on WUE_(inst)and WUE_(i).The results show that WUE_(inst)values among the four treatments were insignificant.However,compared with the NT stand(35.8μmol·mol^(-1)),WUE_(i)values significantly increased to 41.7μmol·mol^(-1)in the UR,50.1μmol·mol^(-1)in the LT and 46.6μmol·mol^(-1)in HT treatments,largely explained by photosynthetic capacity and soil water content.Understory removal did not change physiological performance(needle water potential and photosynthetic capacity).Thinning increased the net photosynthetic rate(A_n)but not stomatal conductance(g_s)or predawn needle water potential(ψ_(pd)),implying that the improvement in water use efficiency for thinned stands was largely driven by radiation interception than by soil water availability.In general,thinning may be an appropriate management measure to promote P.massoniana WUE to cope with seasonal droughts under future extreme climates.

Cereal-forage crop rotations and irrigation treatment effect on water use efficiency and crops sustainability in Mediterranean environment

Agricultural systems based on crop rotations favour sustainability of cultivation and productivity of the crops. Wheat-forage crops rotations (annual winter binary mixture and perennial alfalfa meadow) combined with irrigation are the agronomical techniques able to better exploit the weather resources in Mediterranean environments. The experiment aimed to study the effect of 18 years of combined effect of irrigation and continuous durum wheat and wheat-forage rotations on productivities of crops and organic matter of topsoil. The experiments were established through 1991-2008 under rainfed and irrigated treatments and emphasized on the effect of irrigation and continuous wheat and wheat-forage crop rotations on water use efficiency and sustainability of organic matter. The effect of irrigation increased 49.1% and 66.9% the dry matter of mixture and meadow, respectively. Continuous wheat rotation reduced seed yield, stability of production, crude protein characteristics of kernel and soil organic matter. The yearly gain in wheat after forage crops was 0.04 t (ha·yr)-1 under rainfed and 0.07 t (ha·yr)-1 under irrigation treatments. The crude protein and soil organic matter of wheat rotations, compared to those of continuous wheat under rainfed and irrigated was increase in term of point percentage by 0.8 and 0.5 in crude protein and 5.1 and 4.4 in organic matter, respectively. The rotations of mixture and meadow under both irrigated treatments increased the point of percentage of organic matter over continuous wheat (9.3.and 8.5 in mixture and 12.5 and 9.5 meadow under rainfed and irrigation, respectively). Irrigation reduce the impact of weather on crop growing reducing water use efficiency (mean over rotations) for dry mater production (15.5 in meadow and 17.5 in mixture [L water (kg·dry·matter)-1 ]) and wheat seed yield. The effect of agronomic advantages achieved by forage crops in topsoil expire its effect after three years of continuous wheat rotation.

Soil Carbon Sequestration,Water Use Efficiency(WUE) and Biological Nitrogen Fixation(BNF) Under Conservation Agriculture in Rain-fed Dry Area of North-west Pakistan

Land degradation,unbalanced nutrition,change in climate and its extreme variability are the factors affecting the sustainability of agriculture and food security.In North-west Pakistan,more than 50%of the cultivated area is rain-fed and the crop productivity is low.Conservation agriculture reduces greenhouse gas emissions by enhancing soil carbon sequestration and then improved soil fertility,WUE and crop productivity.A field experiment

Exogenous application of glycine betaine improved water use efficiency in winter wheat(Triticum aestivum L.) via modulating photosynthetic efficiency and antioxidative capacity under conventional and limited irrigation conditions

Improving water use efficiency(WUE)is an important subject in agricultural irrigation for alleviating the scarcity of water resources in semiarid regions of the North China Plain.Moreover,glycine betaine(GB)is one of the most effective compatible solutes synthesized naturally in plants for enhancing stress tolerance under abiotic stress,but little information is available on the involvement of GB in regulating crop WUE under field conditions.This study was conducted to explore the role of exogenously applied GB in improving WUE and plant physiological and biochemical responses inwinterwheat subjected to conventional or limited irrigation during the 2015–2016 and 2016–2017 growing seasons.Exogenous application of GB significantly enhanced antioxidant enzyme activities and reduced the accumulation ofmalondialdehyde and hydrogen peroxide under limited irrigation conditions.Furthermore,GB-treated plantsmaintained higher leaf relative water content andmembrane stability,which led to higher chlorophyll content and gas exchange attributes for better intrinsic and instantaneouswater use efficiencies compared to control plants under limited irrigation conditions.GB-treated plants had higher indole-acetic acid and zeatin riboside levels but lower ABA levels compared to control plants under conventional and limited irrigation conditions.Additionally,GB enhanced the grain filling rate and duration,grain number per spike,and final grainweight,which resulted in higher grain yield compared to the control.Interestingly,GB significantly improved the integrative and photosynthetic WUE under conventional and limited irrigation conditions,although GB treatment did not markedly affect total water consumption.These results suggest the involvement of GB in improving WUEs in winter wheat by modulating hormonal balance,membrane stability,photosynthetic performance and antioxidant systems to maintain higher grain yield under conventional and limited irrigation conditions.

Effect of pruning intensity on soil moisture and water use efficiency in jujube(Ziziphus jujube Mill.) plantations in the hilly Loess Plateau Region, China

Jujube(Ziziphus jujube Mill.)is a traditional economic forest crop and is widely cultivated in hilly areas of the Loess Plateau,China.However,soil desiccation was discovered in jujube plantations.Pruning is recognized as a water-saving method that can reduces soil water consumption.In this study,we monitored the jujube plots with control(CK),light(C1),medium(C2)and high(C3)pruning intensities during the jujube growing period of 2012-2015 to explore the effect of pruning intensity on soil moisture and water use efficiency(WUE)of jujube plantations in the hilly Loess Plateau Region.The results showed that pruning is an effective method for soil water conservation in jujube plantations.Soil moisture increased with increasing pruning intensity during the jujube growing period of 2012-2015.C1,C2 and C3 pruning intensities increased soil water storage by 6.1-18.3,14.4-40.0 and 24.3-63.3 mm,respectively,compared to CK pruning intensity.Pruning promoted soil moisture infiltration to deeper soil layer.Soil moisture infiltrated to soil depths of 240,280 and>300 cm under C3 pruning intensity,220,260 and 260 cm under C2 pruning intensity,200,240 and 220 cm under C1 pruning intensity,and 180,200 and 160 cm under CK pruning intensity in 2013,2014 and 2015,respectively.Soil water deficit was alleviated by higher pruning intensity.In 2013-2015,soil water change was positive under C2(6.4 mm)and C3(26.8 mm)pruning intensities but negative under C1(-20.5 mm)and CK(-40.6 mm)pruning intensities.Moreover,pruning significantly improved fresh fruit yield and WUE of jujube plants.Fresh fruit yields were highest under C1 pruning intensity with the values of 6897.1-13,059.3 kg/hm^2,which were 2758.4-4712.8,385.7-1432.1 and 802.8-2331.5 kg/hm2 higher than those under CK,C2,and C3 pruning intensities during the jujube growing period of 2012-2015,respectively.However,C3 pruning intensity had the highest WUE values of 2.92-3.13 kg/m3,which were 1.6-2.0,1.1-1.2 and 1.0-1.1 times greater than those under CK,C1 and C2 pruning intensities,respectively.Therefore,C3 pruning intensity is recommended to jujube plantations for its economic and ecological benefits.These results provide an alternative strategy to mitigate soil desiccation in jujube plantations in the hilly Loess Plateau Region,which is critical for sustainable cultivation of economic forest trees in this region.

Interaction of Carbon Dioxide Enrichment and Soil Moisture on Photosynthesis, Transpiration, and Water Use Efficiency of Soybean

Soybean (Glycine max (L.) Merrill) is one of the most important oil and protein sources in the world. Interactive effect of elevated carbon dioxide (CO2) and soil water availability potentially impact future food security of the world under climate change. A rhizotron growth chamber experiment was conducted to study soil moisture interactions with elevated CO2 on gaseous exchange parameters of soybean under two CO2 concentrations (380 and 800 μmol·mol-1) with three soil moisture levels. Elevated CO2 decreased photosynthetic rate (11.1% and 10.8%), stomatal conductance (40.5% and 36.0%), intercellular CO2 concentration (16.68% and 12.28%), relative intercellular CO2 concentration (17.4% and 11.2%), and transpiration rate (43.6% and 39%) at 42 and 47 DAP. This down-regulation of photosynthesis was probably caused by low leaf nitrogen content and decrease in uptake of nutrients due to decrease in stomatal conductance and transpiration rate. Water use efficiency (WUE) increased under elevated CO2 because increase in total dry weight of plant was greater than that of water use under high CO2 conditions. Plants under normal and high soil moisture levels had significantly higher photosynthetic rate (7% to 16%) favored by optimum soil moisture content and high specific water content of soybean plants. Total dry matter production was significantly high when plants grown under elevated CO2 with normal (74.3% to 137.3%) soil moisture level. Photosynthetic rate was significantly and positively correlated with leaf conductance and intercellular CO2 concentration but WUE was significantly negatively correlated with leaf conductance, intercellular CO2 concentration and transpiration rate. However, the effect of high CO2 on plants depends on availability of nutrients and soil moisture for positive feedback from CO2 enrichment.

Arbuscular mycorrhizal fungi improve biomass, photosynthesis, and water use efficiency of Opuntia ficus-indica (L.) Miller under different water levels

Opuntia ficus-indica(L.)Miller is a CAM(crassulacean acid metabolism)plant with an extraordinary capacity to adapt to drought stress by its ability to fix atmospheric CO_(2) at nighttime,store a significant amount of water in cladodes,and reduce root growth.Plants that grow in moisture-stress conditions with thick and less fine root hairs have a strong symbiosis with arbuscular mycorrhizal fungi(AMF)to adapt to drought stress.Water stress can limit plant growth and biomass production,which can be rehabilitated by AMF association through improved physiological performance.The objective of this study was to investigate the effects of AMF inoculations and variable soil water levels on the biomass,photosynthesis,and water use efficiency of the spiny and spineless O.ficus-indica.The experiment was conducted in a greenhouse with a full factorial experiment using O.ficus-indica type(spiny or spineless),AMF(presence or absence),and four soil water available(SWA)treatments through seven replications.Water treatments applied were 0%–25%SWA(T1),25%–50%SWA(T2),50%–75%SWA(T3),and 75%–100%SWA(T4).Drought stress reduced biomass and cladode growth,while AMF colonization significantly increased the biomass production with significant changes in the physiological performance of O.ficus-indica.AMF presence significantly increased biomass of both O.ficus-indica plant types through improved growth,photosynthetic water use efficiency,and photosynthesis.The presence of spines on the surface of cladodes significantly reduced the rate of photosynthesis and photosynthetic water use efficiency.Net photosynthesis,photosynthetic water use efficiency,transpiration,and stomatal conductance rate significantly decreased with increased drought stress.Under drought stress,some planted mother cladodes with the absence of AMF have not established daughter cladodes,whereas AMF-inoculated mother cladodes fully established daughter cladodes.AMF root colonization significantly increased with the decrease of SWA.AMF caused an increase in biomass production,increased tolerance to drought stress,and improved photosynthesis and water use efficiency performance of O.ficus-indica.The potential of O.ficus-indica to adapt to drought stress is controlled by the morpho-physiological performance related to AMF association.

Controlled drainage in the Nile River delta of Egypt:a promising approach for decreasing drainage off-site effects and enhancing yield and water use efficiency of wheat

North Africa is one of the most regions impacted by water shortage.The implementation of controlled drainage(CD)in the northern Nile River delta of Egypt is one strategy to decrease irrigation,thus alleviating the negative impact of water shortage.This study investigated the impacts of CD at different levels on drainage outflow,water table level,nitrate loss,grain yield,and water use efficiency(WUE)of various wheat cultivars.Two levels of CD,i.e.,0.4 m below the soil surface(CD-0.4)and 0.8 m below the soil surface(CD-0.8),were compared with subsurface free drainage(SFD)at 1.2 m below the soil surface(SFD-1.2).Under each drainage treatment,four wheat cultivars were grown for two growing seasons(November 2018–April 2019 and November 2019–April 2020).Compared with SFD-1.2,CD-0.4 and CD-0.8 decreased irrigation water by 42.0%and 19.9%,drainage outflow by 40.3%and 27.3%,and nitrate loss by 35.3%and 20.8%,respectively.Under CD treatments,plants absorbed a significant portion of their evapotranspiration from shallow groundwater(22.0%and 8.0%for CD-0.4 and CD-0.8,respectively).All wheat cultivars positively responded to CD treatments,and the highest grain yield and straw yield were obtained under CD-0.4 treatment.Using the initial soil salinity as a reference,the soil salinity under CD-0.4 treatment increased two-fold by the end of the second growing season without negative impacts on wheat yield.Modifying the drainage system by raising the outlet elevation and considering shallow groundwater contribution to crop evapotranspiration promoted water-saving and WUE.Different responses could be obtained based on the different plant tolerance to salinity and water stress,crop characteristics,and growth stage.Site-specific soil salinity management practices will be required to avoid soil salinization due to the adoption of long-term shallow groundwater in Egypt and other similar agroecosystems.

Changes in Water Use Efficiency Caused by Climate Change,CO_(2) Fertilization,and Land Use Changes on the Tibetan Plateau

Terrestrial ecosystem water use efficiency(WUE)is an important indicator for coupling plant photosynthesis and transpiration,and is also a key factor linking the carbon and water cycles between the land and atmosphere.However,under the combination of climate change and human intervention,the change in WUE is still unclear,especially on the Tibetan Plateau(TP).Therefore,satellite remote sensing data and process-based terrestrial biosphere models(TBMs)are used in this study to investigate the spatiotemporal variations of WUE over the TP from 2001 to 2010.Then,the effects of land use and land cover change(LULCC)and CO_(2) fertilization on WUE from 1981-2010 are assessed using TBMs.Results show that climate change is the leading contributor to the change in WUE on the TP,and temperature is the most important factor.LULCC makes a negative contribution to WUE(-20.63%),which is greater than the positive contribution of CO_(2) fertilization(11.65%).In addition,CO_(2) fertilization can effectively improve ecosystem resilience on the TP.On the northwest plateau,the effects of LULCC and CO_(2) fertilization on WUE are more pronounced during the driest years than the annual average.These findings can help researchers understand the response of WUE to climate change and human activity and the coupling of the carbon and water cycles over the TP.

Water use efficiency and yield of winter wheat under different irrigation regimes in a semi-arid region

In irrigation schemes under rotational water supply in semiarid region, the water allocation and irrigation scheduling are often based on a fixed-area proportionate water depth with every irrigation cycle irrespective of crops and their growth stages, for an equitable water supply. An experiment was conducted during the 2004- 2005 season in Haouz irrigated area in Morocco, which objective was i) to evaluate the effects of the surface irrigation scheduling method (existing rule) adopted by the irrigation agency on winter wheat production compared to a full irrigation method and ii) to evaluate drip irrigation versus surface irrigation impacts on water saving and yield of winter wheat. The methodology was based on the FAO-56 dual approach for the surface irrigation scheduling. Ground measurements of the Normalized Difference Vegetation Index (NDVI) were used to derive the basal crop coefficient and the vegetation fraction cover. The simple FAO-56 approach was used for drip irrigation scheduling. For surface irrigation, the existing rule approach resulted in yield and WUE reductions of 22% and 15%, respectively, compared with the optimized irrigation scheduling proposed by the FAO-56 for full irrigation treatment. This revealed the negative effects of the irrigation schedules adopted in irrigation schemes under rotational water supply on crops productivity. It was also demon-strated that drip irrigation applied to wheat was more efficient with 20% of water saving in comparison with surface irrigation (full irrigation treatment). Drip irrigation gives also higher wheat yield compared to surface irrigation (+28% and +52% for full irrigation and existing rule treatments respectively). The same improvement was observed for water use efficiency (+24 and +59% respectively).

Foliar δ^13C Values and Water Use Efficiency ofSemi-arid Plants in Horqin Sandy Land, China

Water was a key factor restricting the plantation in sandy areas. Foliar δ^(13)C value regarded as an indicator of longterm water use efficiency(WUE) of plants was generally used to evaluate the adaptability of plants to arid and semi-arid environment. In Horqin sandy land of China, the foliar δ^(13)C values of 114 species in 35 families naturally growing in the area, and 15 species of sand-fixing trees and shrubs were measured and analyzed in 2012. The results showed that 97 species in all 114 species were C_3 plants and only 17 species were C_4 plants. Most C_4 plants belonged to Gramineae family. The foliar δ^(13)C value of C_3 plants was between -25.000 and -31.075‰ with an average of-28.226‰, while those of C_4 plants between -12.578 and -16.334‰, with an average of -13.678‰. The δ^(13)C values of mature leaves collected in August were averagely 0.85‰ less than that of juvenile leaves collected in June. The foliar δ^(13)C values and WUE of 15 sand-fixing tree species in Horqin sandy land ranked in the order as: S.vulgaris >H.rhamnoides >C.ovata >P.mongolica >T.chinensis >R.typhina >S.matsudana >E.angustifolia >U.pumila >S.gordejevii >X.sorbifolia >C.microphylla >H.fruticosum >C.korshinskii >E.bungeanus.

Canopy morphological changes and water use efficiency in winter wheat under different irrigation treatments

Water is a key limiting factor in agriculture. Water resource shortages have become a serious threat to global food security. The development of water-saving irrigation techniques based on crop requirements is an important strategy to resolve water scarcity in arid and semi-arid regions. In this study, field experiments with winter wheat were performed at Wuqiao Experiment Station, China Agricultural University in two growing seasons in 2013-2015 to help develop such techniques. Three irrigation treatments were tested: no-irrigation(i.e., no water applied after sowing), limited-irrigation(i.e., 60 mm of water applied at jointing), and sufficient-irrigation(i.e., a total of 180 mm of water applied with 60 mm at turning green, jointing and anthesis stages, respectively). Leaf area index(LAI), light transmittance(LT), leaf angle(LA), transpiration rate(Tr), specific leaf weight, water use efficiency(WUE), and grain yield of winter wheat were measured. The highest WUE of wheat in the irrigated treatments was found under limited-irrigation and grain yield was only reduced by a small amount in this treatment compared to the sufficient irrigation treatment. The LAI and LA of wheat plants was lower under limited irrigation than sufficient irrigation, but canopy LT was greater. Moreover, the specific leaf weight of winter wheat was significantly lower under sufficient than limited irrigation conditions, while the leaf Tr was significantly higher. Correlation analysis showed that the increased LAI was associated with an increase in the leaf Tr, but the specific leaf weight had the opposite relationship with transpiration. Optimum WUE occurred over a reasonable range in leaf Tr. In conclusion, reduced irrigation can optimize wheat canopies and regulate water consumption, with only small reductions in final yield, ultimately leading to higher wheat WUE and water saving in arid and semi-arid regions.

Effect of pulse drip irrigation and mulching systems on yield, quality traits and irrigation water use efficiency of soybean under sandy soil conditions

Two field experiments were carried out during growing seasons 2010 and 2011, it executed in research farm of national research center in Nubaryia region, Egypt to study the effect of pulse drip irrigation and mulching systems for saving water, increasing and improving yield of soybean. The study factors were, pulse drip irrigation technology (adding of daily water requirements on 4 times, 8 times, 12 times compared with adding of daily water requirements on 1 time) and mulching systems (covering the soil with black plastic mulch “BPM”, rice straw mulch “RSM” and the control treatment was soil surface without mulch “WM”). The following parameters were studied to evaluate the effect of pulse drip irrigation and mulching systems: 1) Soil moisture distribution in root zone, 2) Growth characters of soybean plant, 3) Yield of soybean, 4) Irrigation water use efficiency of soybean “IWUE soybean”, and 5) Oil content and oil yield, 6) Protein content and protein yield, 7) Economical parameter. According to the economical view and the results of statistical analysis for effect of pulse drip irrigation and mulching systems on yield, quality traits and IWUE soybean indicated that, applying the irrigation requirements on 8 pulses/day with using BPM is the best conditions because under these conditions was occurred the highest value of yield, quality traits and IWUE soybean and there was significant deference between this case and other treatments. Where, pulse irrigation technique increase from water movement in horizontal direction than vertical direction hence improve from soil moisture distribution and wetted soil volume in root zone and using BPM decrease from evaporation process rate from soil surface hence decreasing of salts accumulation in addition to decreasing of weed growth in the root zone. All traits at AIR on 12 pulses/ day are decreased by increasing of pulses, this may be due to irrigation water was very small with every pulse at AIR on 12 pulses/day in addition increasing the total time of time-off, this mean, un-sufficient application for irrigation water to remove water stress in the root zone.

From leaf to whole-plant water use efficiency(WUE)in complex canopies:Limitations of leaf WUE as a selection target

Plant water use efficiency(WUE) is becoming a key issue in semiarid areas, where crop production relies on the use of large volumes of water. Improving WUE is necessary for securing environmental sustainability of food production in these areas. Given that climate change predictions include increases in temperature and drought in semiarid regions,improving crop WUE is mandatory for global food production. WUE is commonly measured at the leaf level, because portable equipment for measuring leaf gas exchange rates facilitates the simultaneous measurement of photosynthesis and transpiration. However,when those measurements are compared with daily integrals or whole-plant estimates of WUE, the two sometimes do not agree. Scaling up from single-leaf to whole-plant WUE was tested in grapevines in different experiments by comparison of daily integrals of instantaneous water use efficiency [ratio between CO2assimilation(AN) and transpiration(E); AN/E] with midday AN/E measurements, showing a low correlation, being worse with increasing water stress. We sought to evaluate the importance of spatial and temporal variation in carbon and water balances at the leaf and plant levels. The leaf position(governing average light interception) in the canopy showed a marked effect on instantaneous and daily integrals of leaf WUE. Night transpiration and respiration rates were also evaluated, as well as respiration contributions to total carbon balance. Two main components were identified as filling the gap between leaf and whole plant WUE: the large effect of leaf position on daily carbon gain and water loss and the large flux of carbon losses by dark respiration. These results show that WUE evaluation among genotypes or treatments needs to be revised.

Paludification reduces black spruce growth rate but does not alter tree water use efficiency in Canadian boreal forested peatlands

Background:Black spruce(Picea mariana(Mill.)BSP)-forested peatlands are widespread ecosystems in boreal North America in which peat accumulation,known as the paludification process,has been shown to induce forest growth decline.The continuously evolving environmental conditions(e.g.,water table rise,increasing peat thickness)in paludified forests may require tree growth mechanism adjustments over time.In this study,we investigate tree ecophysiological mechanisms along a paludification gradient in a boreal forested peatland of eastern Canada by combining peat-based and tree-ring analyses.Carbon and oxygen stable isotopes in tree rings are used to document changes in carbon assimilation rates,stomatal conductance,and water use efficiency.In addition,paleohydrological analyses are performed to evaluate the dynamical ecophysiological adjustments of black spruce trees to site-specific water table variations.Results:Increasing peat accumulation considerably impacts forest growth,but no significant differences in tree water use efficiency(iWUE)are found between the study sites.Tree-ring isotopic analysis indicates no iWUE decrease over the last 100 years,but rather an important increase at each site up to the 1980 s,before iWUE stabilized.Surprisingly,inferred basal area increments do not reflect such trends.Therefore,iWUE variations do not reflect tree ecophysiological adjustments required by changes in growing conditions.Local water table variations induce no changes in ecophysiological mechanisms,but a synchronous shift in iWUE is observed at all sites in the mid-1980 s.Conclusions:Our study shows that paludification induces black spruce growth decline without altering tree water use efficiency in boreal forested peatlands.These findings highlight that failing to account for paludification-related carbon use and allocation could result in the overestimation of aboveground biomass production in paludified sites.Further research on carbon allocation strategies is of utmost importance to understand the carbon sink capacity of these widespread ecosystems in the context of climate change,and to make appropriate forest management decisions in the boreal biome.

Effects of Different Tillage Systems on Soil Properties,Root Growth,Grain Yield,and Water Use Efficiency of Winter Wheat (Triticum aestivum L.) in Arid Northwest China

Studies on root development, soil physical properties, grain yield, and water-use efficiency are important for identifying suitable soil management practices for sustainable crop production. A field experiment was conducted from 2006 through 2008 in arid northwestern China to determine the effects of four tillage systems on soil properties, root development, water-use efficiency, and grain yield of winter wheat （Triticum aestivum L.）. The cultivar Fan 13 was grown under four tillage systems：conventional tillage （CT） without wheat stubble, no-tillage without wheat stubble mulching （NT）, no-tillage with wheat stubble standing （NTSS）, and no-tillage with wheat stubble mulching （NTS）. The soil bulk density （BD） under CT system increased gradually from sowing to harvest, but that in NT, NTSS, and NTS systems had little change. Compared to the CT system, the NTSS and NTS systems improved total soil water storage （0-150 cm） by 6.1-9.6 and 10.5- 15.3% before sowing, and by 2.2-8.9 and 13.0-15.1% after harvest, respectively. The NTSS and NTS systems also increased mean dry root weight density （DRWD） as compared to CT system. The NTS system significantly improved water-use efficiency by 17.2-17.5% and crop yield by 15.6-16.8%, and the NTSS system improved that by 7.8-9.6 and 7.0-12.8%, respectively, compared with the CT system. Our results suggested that Chinese farmers should consider adopting conservation tillage practices in arid northwestern China because of benefits to soil bulk density, water storage, root system, and winter wheat yield.

Statistical Analysis of Leaf Water Use Efficiency and Physiology Traits of Winter Wheat Under Drought Condition

Five statistical methods including simple correlation, multiple linear regression, stepwise regression, principal components, and path analysis were used to explore the relationship between leaf water use efficiency （WUE） and physiological traits （photosynthesis rate, stomatal conductance, transpiration rate, intercellular CO2 concentration, etc.） of 29 wheat cultivars. The results showed that photosynthesis rate, stomatal conductance, and transpiration rate were the most important leaf WUE parameters under drought condition. Based on the results of statistical analyses, principal component analysis could be the most suitable method to ascertain the relationship between leaf WUE and relative physiological traits. It is reasonable to assume that high leaf WUE wheat could be obtained by selecting breeding materials with high photosynthesis rate, low transpiration rate, and stomatal conductance under dry area.

Mulching mode and planting density affect canopy interception loss of rainfall and water use efficiency of dryland maize on the Loess Plateau of China

High and efficient use of limited rainwater resources is of crucial importance for the crop production in arid and semi-arid areas. To investigate the effects of different soil and crop management practices（i.e., mulching mode treatments： flat cultivation with non-mulching, flat cultivation with straw mulching, plastic-covered ridge with bare furrow and plastic-covered ridge with straw-covered furrow; and planting density treatments： low planting density of 45,000 plants/hm^2, medium planting density of 67,500 plants/hm^2 and high planting density of 90,000 plants/hm^2） on rainfall partitioning by dryland maize canopy, especially the resulted net rainfall input beneath the maize canopy, we measured the gross rainfall, throughfall and stemflow at different growth stages of dryland maize in 2015 and 2016 on the Loess Plateau of China. The canopy interception loss was estimated by the water balance method. Soil water storage, leaf area index, grain yield（as well as it components） and water use efficiency of dryland maize were measured or calculated. Results showed that the cumulative throughfall, cumulative stemflow and cumulative canopy interception loss during the whole growing season accounted for 42.3%–77.5%, 15.1%–36.3% and 7.4%–21.4% of the total gross rainfall under different treatments, respectively. Soil mulching could promote the growth and development of dryland maize and enhance the capability of stemflow production and canopy interception loss, thereby increasing the relative stemflow and relative canopy interception loss and reducing the relative throughfall. The relative stemflow and relative canopy interception loss generally increased with increasing planting density, while the relative throughfall decreased with increasing planting density. During the two experimental years, mulching mode had no significant influence on net rainfall due to the compensation between throughfall and stemflow, whereas planting density significantly affected net rainfall. The highest grain yield and water use efficiency of dryland maize were obtained under the combination of medium planting density of 67,500 plants/hm^2 and mulching mode of plastic-covered ridge with straw-covered furrow. Soil mulching can reduce soil evaporation and retain more soil water for dryland maize without reducing the net rainfall input beneath the maize canopy, which may alleviate the contradiction between high soil water consumption and insufficient rainfall input of the soil. In conclusion, the application of medium planting density（67,500 plants/hm^2） under plastic-covered ridge with bare furrow is recommended for increasing dryland maize production on the Loess Plateau of China.

Effects of Non-flooded Cultivation with Straw Mulching on Rice Agronomic Traits and Water Use Efficiency

A field experiment was conducted to study water use efficiency and agronomic traits in rice cultivated in flooded soil and non-flooded soils with and without straw mulching. The total amount of water used by rice under flooded cultivation （FC） was 2.42 and 3.31 times as much as that by rice under the non-flooded cultivation with and without straw mulching, respectively. The average water seepage was 13 560 m^3/ha under the flooded cultivation, 4 750 m^3/ha under the non-flooded cultivation without straw mulching （ZM） and 4 680 m^3/ha under non-flooded cultivation with straw mulching （SM）. The evapotranspiration in the SM treatment was only 38.2% and 63.6% of the FC treatment and ZM treatment, respectively. Compared with the ZM treatment, straw mulching significantly increased leaf area per plant, main root length, gross root length and root dry weight per plant of rice. The highest grain yield under the SM treatment （6 747 kg/ha） was close to the rice cultivated in flooded soil （6 811.5 kg / ha）. However, the yield under the ZM treatment （4 716 kg/ha） was much lower than that under the FS treatment and SM treatment. The order of water use efficiency and irrigation water use efficiency were both as follows： SM〉 ZM〉 FC.