Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the tern...Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.展开更多
Capillary chromatography using an untreated open tubular capillary tube and a ternary solvent mixture consisting of water-hydrophilic/hydrophobic organic solvent as a carrier solution has been developed. The system is...Capillary chromatography using an untreated open tubular capillary tube and a ternary solvent mixture consisting of water-hydrophilic/hydrophobic organic solvent as a carrier solution has been developed. The system is called tube radical distribution chromatography (TRDC). Separation performance of the TRDC system using a fused-silica capillary tube was examined through the phase diagram for the ternary water-acetonitrile-ethyl acetate solvent mixture. The TRDC system required homogeneous carrier solutions with solvent component ratios around the boundary curve between homogeneous and heterogeneous solution in the phase diagram. The data obtained using the fused-silica capillary tube were compared with those obtained using a polytetrafluoroethylene capillary tube in our previous study.展开更多
文摘Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.
文摘Capillary chromatography using an untreated open tubular capillary tube and a ternary solvent mixture consisting of water-hydrophilic/hydrophobic organic solvent as a carrier solution has been developed. The system is called tube radical distribution chromatography (TRDC). Separation performance of the TRDC system using a fused-silica capillary tube was examined through the phase diagram for the ternary water-acetonitrile-ethyl acetate solvent mixture. The TRDC system required homogeneous carrier solutions with solvent component ratios around the boundary curve between homogeneous and heterogeneous solution in the phase diagram. The data obtained using the fused-silica capillary tube were compared with those obtained using a polytetrafluoroethylene capillary tube in our previous study.
