Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of th...Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.展开更多
Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in m...Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon.展开更多
[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of fo...[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of four functional organic materials,namely garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),on the morphological indexes,yield and quality of watermelon were investigated.[Results]Different functional organic materials had different effects on morphological indexes,yield and quality of watermelon.The morphological indexes,nutritional quality indexes and yield of watermelon treated with garlic straw and sheep manure compound(T_(3))and onion straw and chicken manure compound(T_(4))were significantly higher than those treated simply with garlic straw(T_(1))and onion straw(T_(2)),and T_(3)performed relatively better.Compared with treatment T_(2),T_(3)showed a stem diameter,vine length and leaf number increasing by 43.05%,46.69%and 40.77%respectively,central sugar and side sugar contents increasing by 11.72%and 21.90%respectively,and a yield increasing by 42.91%,with significant differences from T_(2).[Conclusions]This study provides technical support for high-quality and high-yielding cultivation of watermelon.展开更多
In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly und...In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.展开更多
Salicylic acid (SA) is a vital plant growth regulator providing promising role in plant development and adopts defense mechanism to abiotic stresses. Salinity is the most limiting abiotic factor for plant development ...Salicylic acid (SA) is a vital plant growth regulator providing promising role in plant development and adopts defense mechanism to abiotic stresses. Salinity is the most limiting abiotic factor for plant development and growth changes in watermelon by producing reactive oxygen species and ultimately oxidative stress. The present study was aimed to investigate the mechanism involved in salt stress alleviation in watermelon (Citrullus lanatus Thanb. Mavs.) through the foliar application of salicylic acid. Watermelon cv. Charleston Gray was grown under moderate saline regime of 3 ds·m-1 NaCl and sprayed with salicylic acid with four level (0.5, 1.0, 2.5 and 5.0 mmol/L) compared along with control. SA @ 5.0 mmol/L was found to be very effective in mitigation of salt stress. SA was found to be very effective in alleviation of salinity stress by produced antioxidants and acted as osmo-regulator.展开更多
The difference between lycopene and phytohormone levels among diploid, triploid and tetraploid plants of two watermelon cultivars during fruit growth and ripening was studied. The expression pattern of five genes(phy...The difference between lycopene and phytohormone levels among diploid, triploid and tetraploid plants of two watermelon cultivars during fruit growth and ripening was studied. The expression pattern of five genes(phytoene synthase(PSY1), phytoene desaturase(PDS), ζ-carotene desaturase(ZDS), carotenoid isomerase(CRTISO), and lycopene β-cyclase(LCYB)) was analyzed in details. In red-fleshed cultivar Mimei, lycopene content increased rapidly from 25 to 35 days after pollination(DAP), and then decreased at 40 DAP. Triploid and tetraploid fruit had higher levels of lycopene than diploid. Moreover, triploid tended to contain more lycopene than tetraploid during fruit growth and ripening stages. However, little amount of lycopene(0–2 mg kg–1 fresh weight(FW)) in yellow-fleshed cultivar Huangmei was found during all fruit development stages. In Mimei, transcript level of PSY1 was generally higher than the other four genes, and LCYB gene expression was the lowest among all five genes being tested. PSY1, CRTISO and LCYB genes showed higher transcript levels in polyploid than in diploid fruit. By contrast, in Huangmei, transcript level of LCYB was not the lowest, but only lower than that of PSY1. PSY1, CRTISO and LCYB genes showed higher expression levels in diploid than in polyploid fruit. In Mimei, the negative correlation between gibberellane(GA) content and lycopene accumulation was determined in all three different ploidy fruits, while a positive correlation was observed between abscisic acid(ABA) content and lycopene accumulation only in diploid watermelon. These results indicated that different lycopene contents in different ploidy watermelons is regulated by the differential transcription expression of the lycopene metabolic genes and phytohormones.展开更多
[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric ...[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid (FA) as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.展开更多
Two field experiments were carried out at Akure (7oN, 5o101E) in the rainforest zone of Nigeria in 2006 and 2007 to determine the effectiveness of neem leaf, woodash and modified neem leaf extracts as fertilizer sourc...Two field experiments were carried out at Akure (7oN, 5o101E) in the rainforest zone of Nigeria in 2006 and 2007 to determine the effectiveness of neem leaf, woodash and modified neem leaf extracts as fertilizer sources in improving soil fertility, growth and yield of maize (Zea mays L) and watermelon (Citrulus lanatus) sole and intercrop. There were six treatments namely, poultry manure, neem leaf extract (sole), woodash extract, modified neem leaf (neem leaf + woodash), NPK 15-15-15 and a control (no fertilizer nor extract), replicated three times and arranged in a randomized complete block design (RCB). The extracts (neem leaf, wood ash and modified neem leaf) were applied at 1200 litres per hectare each, NPK 15-15-15 at 300 kg/ha and poultry was applied at 6t/ha. The results showed that there were significant increases (P 2O), K, Ca, Mg, Na, O.M, P and N compared to NPK 15-15-15 and neem leaf extract. For instance, modified neem leaf extract increased soil pH (H2O), K, Ca, Mg, Na, O.M, P and N by 12.4%, 32.8%, 25%, 23.7%, 19.32%, 17.24% and 20% respectively compared to neem leaf extract under intercrop plot. The high soil K/Ca, K/Mg and P/Mg ratios in the NPK 15-15-15 fertilizer treatment led to an imbalance in the supply of P, K, Ca and Mg nutrients to maize and watermelon crops. The least values for growth, yield and soil parameters were recorded under the control treatment. In these experiments, modified neem leaf extract (woodash + neem leaf extracts) applied at 1200 litres/ha was the most effective in improving soil fertility, growth and yield of maize and watermelon (sole and intercrop) and could substitute for 6 tons per hectare of poultry manure and 300kg/ha of NPK 15-15-15 fertilizer.展开更多
Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its qu...Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its quality change.In this experiment,seed watermelon kernels were used as research objects,and the effects of 2-Azobis(2-amidinopropane)dihydrochloride(AAPH)on seed watermelon kernel protein isolates(WMP)were investigated.The structure and digestion characteristics of WMP after oxidation were studied.The results showed that with the increase of AAPH concentration(0.05−5 mol/L),WMP showed obvious aggregation,and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL.The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g,α-helix decreased andβ-sheet decreased in secondary structure,and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g,enthalpy(H)and denaturation temperature increased(Td)(P<0.05).By mass spectrometry results of simulated gastric digestion products,it was found that oxidation adversely affected the digestion characteristics of WMP.It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel.展开更多
An experiment was conducted to examine probable impact of location and handling on the fungi commonly associated with rotting watermelon (Citrullus lanatus). The rotting watermelon samples were obtained from two vendo...An experiment was conducted to examine probable impact of location and handling on the fungi commonly associated with rotting watermelon (Citrullus lanatus). The rotting watermelon samples were obtained from two vendors each from five different markets (Bodija, Sango, Mokola, Eleyele, Agbowo and Ojoo) in Ibadan, Oyo state. They were placed in different sterile sample bags which were labeled appropriately before bringing them to the Pathology Laboratory, Department of Botany, University of Ibadan. Isolation of fungi from the rotting samples was carried out using pour plate method on acidified Potato Dextrose Agar (APDA). Pathogenicity test was also conducted for the isolated fungi. All experiments were done in duplicates and all plates were incubated at 25°C ± 2°C for 7 days. Pure cultures were obtained and identified and each isolated fungus was recorded as 1 which means present and 0 which means absent. The data obtained were analyzed using SAS version 9.3 at p ≤ 0.05. A total of three fungi were isolated viz., Aspergillus flavus, Saccharomyces cerevisae and Aspergillus niger. Occurrence of A. niger and A. flavus in samples obtained from Bodija, Mokola, Sango and Ojoo markets were significantly (P ≤ 0.05) higher than those from the other two markets. Occurrence of S. cerevisae in samples obtained from Bodija and Mokola markets were significantly (P ≤ 0.05) higher than those from the other three markets. Location had significant impact on the occurrence of isolated fungi in the rotting watermelons. However, handling by vendors had no significant impact on occurrence of the isolated fungi. Vendors and consumers are advised to be mindful of place of purchase and to also maintain good general hygiene before consumption.展开更多
[Objectives] This study was conducted to find water-soluble fertilizers( WSFs) suitable for watermelon cultivation in Hainan area.[Methods] The effects of three WSFs on watermelon yield and quality were studied.[Resul...[Objectives] This study was conducted to find water-soluble fertilizers( WSFs) suitable for watermelon cultivation in Hainan area.[Methods] The effects of three WSFs on watermelon yield and quality were studied.[Results]Fahai·Jiabao WSF improved watermelon yield by 7.87% compared with the CK,and showed a net increase of income of 538.4 yuan/667 m^2.And it could significantly improve soluble sugar,Vc and protein contents,and could effectively reduce nitrate content.[Conclusions]Fahai·Jiabao WSF is the optimal WSF for integration of water and fertilizer,and has the market prospect of large-area application and extension.展开更多
Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In th...Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In this study, the different developmental stages of flower buds of the watermelon cultivar ’Xinteda Zhengkang 9’ were distinguished by cytological observation, and transcriptome sequencing analysis was performed subsequently. Acetocarmine staining of anthers was performed and the longitudinal and transverse diameters of the unopened male flower buds were measured. Cytological observations of anthers at different developmental stages showed that the anther grew from the tetrad to the mature stage, and the longitudinal and transverse diameters of the flower buds increased. The length of the male flower buds also changed significantly during development. Transcriptome sequencing analysis at four periods, the tetrad(A group), mononuclear(B group), dikaryophase(C group), and mature stages(D group). A total of 16 288 differentially expressed genes(DEGs) were detected in the four stages, with the prolongation of developmental stages, the number of DEGs increased gradually in the comparison groups, there was 2 014, 3 259, 4 628, 1 490, 3 495 and 1 132 DEGs revealed in six comparison groups(A-vs.-B, A-vs.-C, A-vs.-D, B-vs.-C, B-vs.-D, and C-vs.-D), respectively. Gene Ontology(GO) and KEGG enrichment analysis showed that the DEGs were mainly enriched in cellular component and starch and sucrose metabolism, phenylpropanoid biosynthesis and pentose sugar, etc. Finally, we completely screened 59 DEGs in the six comparison groups, interestingly, we found one pollen-specific protein(Cla001608) that was significantly down-regulated(the value of log2 Fold Change up to 17.32), which indicated that it may play an important role in the development of male flowers. This work provides insight into the molecular basis of the developmental stages of male flowers in watermelon and may aid in dominant cross breeding.展开更多
[Objectives]This study was conducted to explore how to improve the waterlogging tolerance of red-seed watermelon through grafting,to provide a theoretical basis for its cultivation in rainy season.[Methods]The effects...[Objectives]This study was conducted to explore how to improve the waterlogging tolerance of red-seed watermelon through grafting,to provide a theoretical basis for its cultivation in rainy season.[Methods]The effects of flooding stress on the growth and root physiological and biochemical characteristics of grafted and own-rooted red-seed watermelon seedlings were studied using Luffa as rootstocks and"Zhongxin 1"red-seed watermelon as scions.[Results]After flooding stress,the biomass and root activity of grafted seedlings of red-seed watermelon were significantly higher than those of own-rooted seedlings.With the prolongation of flooding stress time,the soluble sugar and proline content showed a trend of first increasing and then decreasing,and the grafted seedlings had a larger increase and a smaller decrease,and were always significantly higher than own-rooted seedlings in the same period.The content of malondialdehyde in the root system of grafted seedlings increased first and then decreased,while it continued to increase in own-rooted seedlings,and the increase in own-rooted seedlings was significantly greater than that in grafted seedlings during the same period.[Conclusions]Grafting on Luffa rootstocks could improve waterlogging tolerance of red-seed watermelon.展开更多
Highly prized by consumers, watermelon is rich in water, but also in micronutrients <span style="font-family:Verdana;">including lycopene, pigment responsible for the red color. It is also </span>...Highly prized by consumers, watermelon is rich in water, but also in micronutrients <span style="font-family:Verdana;">including lycopene, pigment responsible for the red color. It is also </span><span style="font-family:Verdana;">a powerful antioxidant which has many virtues including the prevention and </span><span style="font-family:Verdana;">treatment of certain diseases. The transformation into nectar of watermelons </span><span style="font-family:Verdana;">combined with treatment could cause several modifications including the alteration </span><span style="font-family:Verdana;">of coloring. It is in this context that this study focuses on the variation </span><span style="font-family:Verdana;">of the lycopene content in nectars. Thus, nectars of 12<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>Brix and 15<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>Brix </span><span style="font-family:Verdana;">were prepared from three varieties of watermelon (Sugar Baby, Crimson </span><span style="font-family:Verdana;">Sweet and Charleston Gray). To study the stability, two pasteurization scales </span><span style="font-family:Verdana;">(85<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>C/15min and 95<span style="white-space:nowrap;"><span style="font-family:Verdana;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span></span>C/15min) and one sterilization scale (105<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>C/15min) </span><span style="font-family:Verdana;">were applied to the different nectars produced. The results obtained showed </span><span style="font-family:Verdana;">that the Sugar Baby variety is richer in lycopene (24.39 mg</span><span style="font-family:Verdana;white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span><span style="white-space:nowrap;"><span style="font-family:Verdana;">kg</span><span style="color:#4f4f4f;font-family:'-apple-system, font-size:16px;white-space:normal;background-color:#F7F7F7;">-1</span><span style="font-family:Verdana;"></span></span><span style="font-family:Verdana;">) with a higher </span><span style="font-family:Verdana;">pH (5.80). In addition, the study showed, for the Sugar Baby variety, an increase </span><span style="font-family:Verdana;">of lycopene with the addition of sugar and the heat treatment (a maximum </span><span style="font-family:Verdana;">of 42.83 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for SbF12T105). On the other hand, for the Crimson </span><span style="font-family:Verdana;">Sweet and Charleston varieties, the highest rate of lycopene, except the </span><span style="font-family:Verdana;">heat-treated ones, are those formulated at 12<span style="font-family:Verdana;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>B (10.46 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for CrF12T105 </span><span style="font-family:Verdana;">and 18.40 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for ChF12T105). Without any health consequences, the formulation </span><span style="font-family:Verdana;">combined with heat treatment would preserve the lycopene content </span><span style="font-family:Verdana;">of watermelon nectars.</span>展开更多
This paper expounded the current situation and genetic mechanisms of short-vine watermelon breeding from the aspects of material sources,breeding process and genetic characteristics of F_1,hoping to provide a theoreti...This paper expounded the current situation and genetic mechanisms of short-vine watermelon breeding from the aspects of material sources,breeding process and genetic characteristics of F_1,hoping to provide a theoretical basis for short-vine watermelon breeding,and breeding materials for watermelon planting innovation,as well as new opportunities for high-quality and high-yield watermelon.展开更多
The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelo...The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelon, a valued and nutritious fruit, remain largely unknown and their functions have not been characterized. In the present study, 22 ClNF-Y genes in watermelon, 29 CsNF-Y genes in cucumber, and 24CmNF-Y genes in melon were identified based on the whole-genome investigation and their protein properties, gene location, gene structure, motif composition, conserved domain, and evolutionary relationship were investigated. ClNF-YB9 from watermelon and its homologs in cucumber and melon were expressed specifically in seeds. Its expression remained low in the early stages of watermelon seed development,increased at 20 days after pollination(DAP), and peaked at 45–50 DAP. Moreover, the knockout mutant Clnf-yb9 exhibited abnormal leafy cotyledon phenotype, implying its critical role during seed formation.Finally, protein interaction assays showed that ClNF-YB9 interacts with all ClNF-YCs and the ClNF-YB9-YC4 heterodimer was able to recruit a ClNF-YA7 subunit to assemble a complete NF-Y complex, which may function in seed development. This study revealed the structure and evolutionary relationships of the NF-Y gene family in Cucurbitaceae and the novel function of ClNF-YB9 in regulating seed development in watermelon.展开更多
[Objectives]This study was conducted to provide a reliable theoretical basis for the correct formulation of breeding programs for red-seed watermelon and the effective breeding of hybrid offspring.[Methods]With local ...[Objectives]This study was conducted to provide a reliable theoretical basis for the correct formulation of breeding programs for red-seed watermelon and the effective breeding of hybrid offspring.[Methods]With local varieties and inbred lines of red-seed watermelon as test materials,the generalized heritability of fruit number per plant,single-fruit weight,single-fruit seed number,single-fruit seed weight,seed kernel weight,1000-seed weight,kernel-producing ratio,seed production ratio and seed volume were estimated by variance analysis;and the heritability,genetic variation coefficients,and correlation of the nine yield traits in 43 red-seed watermelon varieties were studied.[Results]The generalized heritability of fruit number per plant,single-fruit weight,single-fruit seed number,single-fruit seed weight,seed kernel weight,1000-seed weight,kernel-producing ratio,seed production ratio and seed volume were 12.86%,80.14%,75.96%,74.39%,48.01%,17.12%,24.97%,18.60%,and 37.07%,respectively.The heritability of single-fruit weight,single-fruit seed number and single-fruit seed weight was higher,and early-generation individual selection could achieve a better effect on them;and 1000-seed weight and kernel-producing ratio had a higher coefficient of genetic variation,indicating a high selection potential.[Conclusions]Indirect selection could be achieved for traits such as the single-fruit seed number,single-fruit seed weight,seed kernel weight,and seed volume by selecting the single-fruit weight.In the process of red-seed watermelon breeding,traits with high heritability can be selected in early generations of hybrids,thus playing the role of early-generation orientation.展开更多
[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30...[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30,50,80 and 120 mg/L paclobutrazol at cotyledon expansion stage,and those sprayed with tap water were used as control.[Results]All the treatments of paclobutrazol effectively inhibited the excessive growth of watermelon at seedling stage,significantly enhanced the leaf quality of watermelon seedlings and promoted root growth.The seedlings treated by 50 mg/L paclobutrazol had the best performance compared with the control:a decrease of hypocotyl by 37.9%,an increase of plant dry weight by 46.55%,significantly increased seedling strength index,deep green leaf color,thick leaves,an increase of specific leaf weight by 28.13%,and significantly heavier root weight and longer length.[Conclusions]The results will provide a reference for strong seedling cultivation of small seedless watermelon.展开更多
[Objectives]This study was conducted to select suitable medium-sized watermelon varieties for early spring cultivation in Beijing.[Methods]Seven watermelon varieties were collected,including Beinong Tianjiao 2,Beinong...[Objectives]This study was conducted to select suitable medium-sized watermelon varieties for early spring cultivation in Beijing.[Methods]Seven watermelon varieties were collected,including Beinong Tianjiao 2,Beinong Tianjiao,Hongfuji,Yongfeng 1,Beinong Futian,Beinong Shijia,and Beinong Jinxiu,and Jingxin 2 was used as the control check(CK).The growth period,morphological traits,yield traits and fruit quality of the tested varieties were investigated.[Results]Beinong Tianjiao 2 had an earlier maturation stage,a fruit development period of 38 d and a total growth period of 118 d.It showed stable growth,with a single fruit weight of 7.56 kg,a fruit setting rate of 0.99 per plant,and a yield of 83076.9 kg/hm~2,which ranked,respectively,second,first,and second among all tested varieties,and were significantly superior to the CK.Furthermore,this variety had thin and tough peel,a bright red flesh color,high central sugar content,loose and crisp flesh,and a good taste.[Conclusions]It is preliminarily believed that it is suitable for early spring watermelon production in Beijing area.展开更多
[Objectives]This study was conducted to select small seedless watermelon varieties with excellent quality and high yield suitable for planting in Beijing.[Methods]Under the conditions of facility cultivation in Beijin...[Objectives]This study was conducted to select small seedless watermelon varieties with excellent quality and high yield suitable for planting in Beijing.[Methods]Under the conditions of facility cultivation in Beijing,a screening test was conducted on seven small seedless watermelon varieties for growth process,field traits,yield traits,fruit quality,and disease resistance.[Results]Varieties Mitong,Motong and Tianbao Xiaowuzi had a short growth period and relatively stable growth,and bore fruit easily,and their female flowers were closely spaced.Their yields exceeded 60000 kg/hm~2,ranking in the top three among all varieties.The fruit had a thin skin,a bright red flesh color,and a high mass fraction of soluble solids in the center.The flesh was tight or crispy,and tasted good.They also showed good disease resistance.Overall,they showed excellent comprehensive agronomic traits,and are thus suitable for facility cultivation in Beijing and its surrounding areas.[Conclusions]This study provides a theoretical basis for the upgrading of varieties and the increase in production and farmers'income in the suburbs of Beijing.展开更多
基金supported by fundings from the Natural Science Funds for Outstanding Youth of Heilongjiang Province,China(YQ2022C011)the National Natural Science Foundation of China(32172577)+2 种基金the China Agriculture Research System of MOF and MARA,China(CARS-25)the Taishan Industrial Leading Talents Project,China(LJNY202112)the Natural Science Foundation of Heilongjiang Province,China(LH2022C025).
文摘Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.
基金This research was supported by the National Natural Science Foundation of China(32102389,32172602)the Zhongyuan Youth Talent Support Program(ZYQR201912161)+3 种基金the Program for Science&Technology Innovation Talents in Universities of Henan Province(21HASTIT038)the Funding of Joint Research on Agricultural Varietie Improvement of Henan Province(2022010503)the Major Science and Technology Project of Henan Province(221100110400)the Science and Technology Innovation Fund of Henan Agricultural University(KJCX2021A14).
文摘Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon.
基金Supported by Shangqiu Science and Technology Research Project(202405).
文摘[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of four functional organic materials,namely garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),on the morphological indexes,yield and quality of watermelon were investigated.[Results]Different functional organic materials had different effects on morphological indexes,yield and quality of watermelon.The morphological indexes,nutritional quality indexes and yield of watermelon treated with garlic straw and sheep manure compound(T_(3))and onion straw and chicken manure compound(T_(4))were significantly higher than those treated simply with garlic straw(T_(1))and onion straw(T_(2)),and T_(3)performed relatively better.Compared with treatment T_(2),T_(3)showed a stem diameter,vine length and leaf number increasing by 43.05%,46.69%and 40.77%respectively,central sugar and side sugar contents increasing by 11.72%and 21.90%respectively,and a yield increasing by 42.91%,with significant differences from T_(2).[Conclusions]This study provides technical support for high-quality and high-yielding cultivation of watermelon.
基金supported by the earmarked fund for China Agriculture Research System (CARS-25)the Fundamental Research Funds of the Chinese Academy of Agricultural Sciences (Y2018YJ15 and Y2019XK16-03)+1 种基金the Agricultural Science and Technology Innovation Program, Chinese Academy of Agricultural Sciences (CAAS-ASTIP2018-ZFRI)the National Key R&D Program of China (2018YFD0201310)
文摘In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule.
文摘Salicylic acid (SA) is a vital plant growth regulator providing promising role in plant development and adopts defense mechanism to abiotic stresses. Salinity is the most limiting abiotic factor for plant development and growth changes in watermelon by producing reactive oxygen species and ultimately oxidative stress. The present study was aimed to investigate the mechanism involved in salt stress alleviation in watermelon (Citrullus lanatus Thanb. Mavs.) through the foliar application of salicylic acid. Watermelon cv. Charleston Gray was grown under moderate saline regime of 3 ds·m-1 NaCl and sprayed with salicylic acid with four level (0.5, 1.0, 2.5 and 5.0 mmol/L) compared along with control. SA @ 5.0 mmol/L was found to be very effective in mitigation of salt stress. SA was found to be very effective in alleviation of salinity stress by produced antioxidants and acted as osmo-regulator.
基金supported by the National Natural Science Foundation of China (31171979 and 31471893)the Agricultural Science and Technology Innovation Program, China (ASTIP) (CAAS-ASTIP-2017-ZFRI)+1 种基金the earmarked fund for the China Agriculture Research System (CARS-26-03)the Central Public-interest Scientific Institution Basal Research Fund, China (1610192016301 and 1616032017209)
文摘The difference between lycopene and phytohormone levels among diploid, triploid and tetraploid plants of two watermelon cultivars during fruit growth and ripening was studied. The expression pattern of five genes(phytoene synthase(PSY1), phytoene desaturase(PDS), ζ-carotene desaturase(ZDS), carotenoid isomerase(CRTISO), and lycopene β-cyclase(LCYB)) was analyzed in details. In red-fleshed cultivar Mimei, lycopene content increased rapidly from 25 to 35 days after pollination(DAP), and then decreased at 40 DAP. Triploid and tetraploid fruit had higher levels of lycopene than diploid. Moreover, triploid tended to contain more lycopene than tetraploid during fruit growth and ripening stages. However, little amount of lycopene(0–2 mg kg–1 fresh weight(FW)) in yellow-fleshed cultivar Huangmei was found during all fruit development stages. In Mimei, transcript level of PSY1 was generally higher than the other four genes, and LCYB gene expression was the lowest among all five genes being tested. PSY1, CRTISO and LCYB genes showed higher transcript levels in polyploid than in diploid fruit. By contrast, in Huangmei, transcript level of LCYB was not the lowest, but only lower than that of PSY1. PSY1, CRTISO and LCYB genes showed higher expression levels in diploid than in polyploid fruit. In Mimei, the negative correlation between gibberellane(GA) content and lycopene accumulation was determined in all three different ploidy fruits, while a positive correlation was observed between abscisic acid(ABA) content and lycopene accumulation only in diploid watermelon. These results indicated that different lycopene contents in different ploidy watermelons is regulated by the differential transcription expression of the lycopene metabolic genes and phytohormones.
基金Supported by National Space Breeding Project of China(2006HT100113)China Agriculture Research System(CARS-26)Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(GNK2013YZ26)
文摘[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid (FA) as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.
文摘Two field experiments were carried out at Akure (7oN, 5o101E) in the rainforest zone of Nigeria in 2006 and 2007 to determine the effectiveness of neem leaf, woodash and modified neem leaf extracts as fertilizer sources in improving soil fertility, growth and yield of maize (Zea mays L) and watermelon (Citrulus lanatus) sole and intercrop. There were six treatments namely, poultry manure, neem leaf extract (sole), woodash extract, modified neem leaf (neem leaf + woodash), NPK 15-15-15 and a control (no fertilizer nor extract), replicated three times and arranged in a randomized complete block design (RCB). The extracts (neem leaf, wood ash and modified neem leaf) were applied at 1200 litres per hectare each, NPK 15-15-15 at 300 kg/ha and poultry was applied at 6t/ha. The results showed that there were significant increases (P 2O), K, Ca, Mg, Na, O.M, P and N compared to NPK 15-15-15 and neem leaf extract. For instance, modified neem leaf extract increased soil pH (H2O), K, Ca, Mg, Na, O.M, P and N by 12.4%, 32.8%, 25%, 23.7%, 19.32%, 17.24% and 20% respectively compared to neem leaf extract under intercrop plot. The high soil K/Ca, K/Mg and P/Mg ratios in the NPK 15-15-15 fertilizer treatment led to an imbalance in the supply of P, K, Ca and Mg nutrients to maize and watermelon crops. The least values for growth, yield and soil parameters were recorded under the control treatment. In these experiments, modified neem leaf extract (woodash + neem leaf extracts) applied at 1200 litres/ha was the most effective in improving soil fertility, growth and yield of maize and watermelon (sole and intercrop) and could substitute for 6 tons per hectare of poultry manure and 300kg/ha of NPK 15-15-15 fertilizer.
基金This study was supported by National Natural Science Foundation of China(No:31760477)Beijing Advanced Innovation Center for Food Nutrition and Human Health(No:20181007)Youth Science and Technology Innovation,Leader in Corps(No:2016BC001).
文摘Seed watermelon kernel is a typical complex food with high fat and protein contents.During storage and processing,it is often affected by various factors to undergo interactions between components,which lead to its quality change.In this experiment,seed watermelon kernels were used as research objects,and the effects of 2-Azobis(2-amidinopropane)dihydrochloride(AAPH)on seed watermelon kernel protein isolates(WMP)were investigated.The structure and digestion characteristics of WMP after oxidation were studied.The results showed that with the increase of AAPH concentration(0.05−5 mol/L),WMP showed obvious aggregation,and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL.The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g,α-helix decreased andβ-sheet decreased in secondary structure,and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g,enthalpy(H)and denaturation temperature increased(Td)(P<0.05).By mass spectrometry results of simulated gastric digestion products,it was found that oxidation adversely affected the digestion characteristics of WMP.It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel.
文摘An experiment was conducted to examine probable impact of location and handling on the fungi commonly associated with rotting watermelon (Citrullus lanatus). The rotting watermelon samples were obtained from two vendors each from five different markets (Bodija, Sango, Mokola, Eleyele, Agbowo and Ojoo) in Ibadan, Oyo state. They were placed in different sterile sample bags which were labeled appropriately before bringing them to the Pathology Laboratory, Department of Botany, University of Ibadan. Isolation of fungi from the rotting samples was carried out using pour plate method on acidified Potato Dextrose Agar (APDA). Pathogenicity test was also conducted for the isolated fungi. All experiments were done in duplicates and all plates were incubated at 25°C ± 2°C for 7 days. Pure cultures were obtained and identified and each isolated fungus was recorded as 1 which means present and 0 which means absent. The data obtained were analyzed using SAS version 9.3 at p ≤ 0.05. A total of three fungi were isolated viz., Aspergillus flavus, Saccharomyces cerevisae and Aspergillus niger. Occurrence of A. niger and A. flavus in samples obtained from Bodija, Mokola, Sango and Ojoo markets were significantly (P ≤ 0.05) higher than those from the other two markets. Occurrence of S. cerevisae in samples obtained from Bodija and Mokola markets were significantly (P ≤ 0.05) higher than those from the other three markets. Location had significant impact on the occurrence of isolated fungi in the rotting watermelons. However, handling by vendors had no significant impact on occurrence of the isolated fungi. Vendors and consumers are advised to be mindful of place of purchase and to also maintain good general hygiene before consumption.
基金Supported by Special Fund for Cultivated Land Improvement in Hainan Province(No.HNGDxf201501)
文摘[Objectives] This study was conducted to find water-soluble fertilizers( WSFs) suitable for watermelon cultivation in Hainan area.[Methods] The effects of three WSFs on watermelon yield and quality were studied.[Results]Fahai·Jiabao WSF improved watermelon yield by 7.87% compared with the CK,and showed a net increase of income of 538.4 yuan/667 m^2.And it could significantly improve soluble sugar,Vc and protein contents,and could effectively reduce nitrate content.[Conclusions]Fahai·Jiabao WSF is the optimal WSF for integration of water and fertilizer,and has the market prospect of large-area application and extension.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-25)the Central Public-interest Scientific Institution Basal Research Fund,China(Y2019XK16-03)+2 种基金the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAASASTIP-2020-ZFRI)the Key Scientific and Technological Project of Henan Province,China(202102110194)the Major Science and Technology Project in Zhengzhou,China(188PCXZX802)。
文摘Watermelon(Citrullus lanatus(Thunb.) Matsum. & Nakai) is an important cucurbit crop grown worldwide. Watermelon fruit quality, fertility, and seed-setting rate are closely related to male flower development. In this study, the different developmental stages of flower buds of the watermelon cultivar ’Xinteda Zhengkang 9’ were distinguished by cytological observation, and transcriptome sequencing analysis was performed subsequently. Acetocarmine staining of anthers was performed and the longitudinal and transverse diameters of the unopened male flower buds were measured. Cytological observations of anthers at different developmental stages showed that the anther grew from the tetrad to the mature stage, and the longitudinal and transverse diameters of the flower buds increased. The length of the male flower buds also changed significantly during development. Transcriptome sequencing analysis at four periods, the tetrad(A group), mononuclear(B group), dikaryophase(C group), and mature stages(D group). A total of 16 288 differentially expressed genes(DEGs) were detected in the four stages, with the prolongation of developmental stages, the number of DEGs increased gradually in the comparison groups, there was 2 014, 3 259, 4 628, 1 490, 3 495 and 1 132 DEGs revealed in six comparison groups(A-vs.-B, A-vs.-C, A-vs.-D, B-vs.-C, B-vs.-D, and C-vs.-D), respectively. Gene Ontology(GO) and KEGG enrichment analysis showed that the DEGs were mainly enriched in cellular component and starch and sucrose metabolism, phenylpropanoid biosynthesis and pentose sugar, etc. Finally, we completely screened 59 DEGs in the six comparison groups, interestingly, we found one pollen-specific protein(Cla001608) that was significantly down-regulated(the value of log2 Fold Change up to 17.32), which indicated that it may play an important role in the development of male flowers. This work provides insight into the molecular basis of the developmental stages of male flowers in watermelon and may aid in dominant cross breeding.
基金Supported by"Watermelon and Muskmelon Germplasm Innovation and Genetic Improvement"Post of Guangxi Bagui Scholars(2016A11)。
文摘[Objectives]This study was conducted to explore how to improve the waterlogging tolerance of red-seed watermelon through grafting,to provide a theoretical basis for its cultivation in rainy season.[Methods]The effects of flooding stress on the growth and root physiological and biochemical characteristics of grafted and own-rooted red-seed watermelon seedlings were studied using Luffa as rootstocks and"Zhongxin 1"red-seed watermelon as scions.[Results]After flooding stress,the biomass and root activity of grafted seedlings of red-seed watermelon were significantly higher than those of own-rooted seedlings.With the prolongation of flooding stress time,the soluble sugar and proline content showed a trend of first increasing and then decreasing,and the grafted seedlings had a larger increase and a smaller decrease,and were always significantly higher than own-rooted seedlings in the same period.The content of malondialdehyde in the root system of grafted seedlings increased first and then decreased,while it continued to increase in own-rooted seedlings,and the increase in own-rooted seedlings was significantly greater than that in grafted seedlings during the same period.[Conclusions]Grafting on Luffa rootstocks could improve waterlogging tolerance of red-seed watermelon.
文摘Highly prized by consumers, watermelon is rich in water, but also in micronutrients <span style="font-family:Verdana;">including lycopene, pigment responsible for the red color. It is also </span><span style="font-family:Verdana;">a powerful antioxidant which has many virtues including the prevention and </span><span style="font-family:Verdana;">treatment of certain diseases. The transformation into nectar of watermelons </span><span style="font-family:Verdana;">combined with treatment could cause several modifications including the alteration </span><span style="font-family:Verdana;">of coloring. It is in this context that this study focuses on the variation </span><span style="font-family:Verdana;">of the lycopene content in nectars. Thus, nectars of 12<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>Brix and 15<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>Brix </span><span style="font-family:Verdana;">were prepared from three varieties of watermelon (Sugar Baby, Crimson </span><span style="font-family:Verdana;">Sweet and Charleston Gray). To study the stability, two pasteurization scales </span><span style="font-family:Verdana;">(85<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>C/15min and 95<span style="white-space:nowrap;"><span style="font-family:Verdana;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span></span>C/15min) and one sterilization scale (105<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>C/15min) </span><span style="font-family:Verdana;">were applied to the different nectars produced. The results obtained showed </span><span style="font-family:Verdana;">that the Sugar Baby variety is richer in lycopene (24.39 mg</span><span style="font-family:Verdana;white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span><span style="white-space:nowrap;"><span style="font-family:Verdana;">kg</span><span style="color:#4f4f4f;font-family:'-apple-system, font-size:16px;white-space:normal;background-color:#F7F7F7;">-1</span><span style="font-family:Verdana;"></span></span><span style="font-family:Verdana;">) with a higher </span><span style="font-family:Verdana;">pH (5.80). In addition, the study showed, for the Sugar Baby variety, an increase </span><span style="font-family:Verdana;">of lycopene with the addition of sugar and the heat treatment (a maximum </span><span style="font-family:Verdana;">of 42.83 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for SbF12T105). On the other hand, for the Crimson </span><span style="font-family:Verdana;">Sweet and Charleston varieties, the highest rate of lycopene, except the </span><span style="font-family:Verdana;">heat-treated ones, are those formulated at 12<span style="font-family:Verdana;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span></span>B (10.46 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for CrF12T105 </span><span style="font-family:Verdana;">and 18.40 mg<span style="white-space:nowrap;"><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"=""><span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#f7f7f7;"="">·</span></span></span>kg-1 for ChF12T105). Without any health consequences, the formulation </span><span style="font-family:Verdana;">combined with heat treatment would preserve the lycopene content </span><span style="font-family:Verdana;">of watermelon nectars.</span>
文摘This paper expounded the current situation and genetic mechanisms of short-vine watermelon breeding from the aspects of material sources,breeding process and genetic characteristics of F_1,hoping to provide a theoretical basis for short-vine watermelon breeding,and breeding materials for watermelon planting innovation,as well as new opportunities for high-quality and high-yield watermelon.
基金supported by the National Youth Talent Program (A279021801)Key-Area R&D Program of Guangdong province (2022B0202060001)+4 种基金Key R&D Program of Shaanxi Province (2023-YBNY-008)the Science and Technology Innovation Team of Shaanxi (2021TD-32)the Natural Science Foundation of Shaanxi Province (2021JM-089, 2022JM-112 and 2022JQ-162)the Key R&D Project from Yangling Seed Industry Innovation Center (K3031322016)the Fundamental Research Fund from Northwest A&F University (2452022111)。
文摘The nuclear factor Y(NF-Y) gene family is a class of transcription factors that are widely distributed in eukaryotes and are involved in various biological processes. However, the NF-Y gene family members in watermelon, a valued and nutritious fruit, remain largely unknown and their functions have not been characterized. In the present study, 22 ClNF-Y genes in watermelon, 29 CsNF-Y genes in cucumber, and 24CmNF-Y genes in melon were identified based on the whole-genome investigation and their protein properties, gene location, gene structure, motif composition, conserved domain, and evolutionary relationship were investigated. ClNF-YB9 from watermelon and its homologs in cucumber and melon were expressed specifically in seeds. Its expression remained low in the early stages of watermelon seed development,increased at 20 days after pollination(DAP), and peaked at 45–50 DAP. Moreover, the knockout mutant Clnf-yb9 exhibited abnormal leafy cotyledon phenotype, implying its critical role during seed formation.Finally, protein interaction assays showed that ClNF-YB9 interacts with all ClNF-YCs and the ClNF-YB9-YC4 heterodimer was able to recruit a ClNF-YA7 subunit to assemble a complete NF-Y complex, which may function in seed development. This study revealed the structure and evolutionary relationships of the NF-Y gene family in Cucurbitaceae and the novel function of ClNF-YB9 in regulating seed development in watermelon.
基金Supported by"Watermelon and Muskmelon Germplasm Innovation and Genetic Improvement"Post of Guangxi Bagui Scholars(2016A11)。
文摘[Objectives]This study was conducted to provide a reliable theoretical basis for the correct formulation of breeding programs for red-seed watermelon and the effective breeding of hybrid offspring.[Methods]With local varieties and inbred lines of red-seed watermelon as test materials,the generalized heritability of fruit number per plant,single-fruit weight,single-fruit seed number,single-fruit seed weight,seed kernel weight,1000-seed weight,kernel-producing ratio,seed production ratio and seed volume were estimated by variance analysis;and the heritability,genetic variation coefficients,and correlation of the nine yield traits in 43 red-seed watermelon varieties were studied.[Results]The generalized heritability of fruit number per plant,single-fruit weight,single-fruit seed number,single-fruit seed weight,seed kernel weight,1000-seed weight,kernel-producing ratio,seed production ratio and seed volume were 12.86%,80.14%,75.96%,74.39%,48.01%,17.12%,24.97%,18.60%,and 37.07%,respectively.The heritability of single-fruit weight,single-fruit seed number and single-fruit seed weight was higher,and early-generation individual selection could achieve a better effect on them;and 1000-seed weight and kernel-producing ratio had a higher coefficient of genetic variation,indicating a high selection potential.[Conclusions]Indirect selection could be achieved for traits such as the single-fruit seed number,single-fruit seed weight,seed kernel weight,and seed volume by selecting the single-fruit weight.In the process of red-seed watermelon breeding,traits with high heritability can be selected in early generations of hybrids,thus playing the role of early-generation orientation.
基金Beijing Innovation Consortium of Special Crops Research System(BAIC04-2022).
文摘[Objectives]The paper was to explore the effects of different concentrations of paclobutrazol on seedling quality of small seedless watermelon in autumn.[Methods]The leaves of watermelon seedlings were sprayed with 30,50,80 and 120 mg/L paclobutrazol at cotyledon expansion stage,and those sprayed with tap water were used as control.[Results]All the treatments of paclobutrazol effectively inhibited the excessive growth of watermelon at seedling stage,significantly enhanced the leaf quality of watermelon seedlings and promoted root growth.The seedlings treated by 50 mg/L paclobutrazol had the best performance compared with the control:a decrease of hypocotyl by 37.9%,an increase of plant dry weight by 46.55%,significantly increased seedling strength index,deep green leaf color,thick leaves,an increase of specific leaf weight by 28.13%,and significantly heavier root weight and longer length.[Conclusions]The results will provide a reference for strong seedling cultivation of small seedless watermelon.
文摘[Objectives]This study was conducted to select suitable medium-sized watermelon varieties for early spring cultivation in Beijing.[Methods]Seven watermelon varieties were collected,including Beinong Tianjiao 2,Beinong Tianjiao,Hongfuji,Yongfeng 1,Beinong Futian,Beinong Shijia,and Beinong Jinxiu,and Jingxin 2 was used as the control check(CK).The growth period,morphological traits,yield traits and fruit quality of the tested varieties were investigated.[Results]Beinong Tianjiao 2 had an earlier maturation stage,a fruit development period of 38 d and a total growth period of 118 d.It showed stable growth,with a single fruit weight of 7.56 kg,a fruit setting rate of 0.99 per plant,and a yield of 83076.9 kg/hm~2,which ranked,respectively,second,first,and second among all tested varieties,and were significantly superior to the CK.Furthermore,this variety had thin and tough peel,a bright red flesh color,high central sugar content,loose and crisp flesh,and a good taste.[Conclusions]It is preliminarily believed that it is suitable for early spring watermelon production in Beijing area.
基金Supported by Special Crop Innovation Team of Beijing(BAIC04-2022)。
文摘[Objectives]This study was conducted to select small seedless watermelon varieties with excellent quality and high yield suitable for planting in Beijing.[Methods]Under the conditions of facility cultivation in Beijing,a screening test was conducted on seven small seedless watermelon varieties for growth process,field traits,yield traits,fruit quality,and disease resistance.[Results]Varieties Mitong,Motong and Tianbao Xiaowuzi had a short growth period and relatively stable growth,and bore fruit easily,and their female flowers were closely spaced.Their yields exceeded 60000 kg/hm~2,ranking in the top three among all varieties.The fruit had a thin skin,a bright red flesh color,and a high mass fraction of soluble solids in the center.The flesh was tight or crispy,and tasted good.They also showed good disease resistance.Overall,they showed excellent comprehensive agronomic traits,and are thus suitable for facility cultivation in Beijing and its surrounding areas.[Conclusions]This study provides a theoretical basis for the upgrading of varieties and the increase in production and farmers'income in the suburbs of Beijing.