SYNTHESIS AND PROPERTIES OF ANILING ANILING AND 0-AMINOBENZENESULFONIC ACID COPOLYMER

Poly(aniline- co-o-aminobenzenesulfonic acid) (PAOABSA) as a water soluble conducting polymerwas synthesized by chemical polymerization. The productivity and the room-temperature conductivity of thecopolymer were measured as a function of the reaction conditions, such as reaction temperature, the ratio ofoxidant to monomer and the degree of sulfonation defined as the ratio of sulfur to nitrogen atoms(S/N). Themain results obtained are summarized as follows : (1) lower reaction temperature (at about 0℃) is favorablefor the enhancement of the room-temperature conductivity of the copolymer; (2) higher content of oxidant isunfavorable for increasing the room-temperature conductivity of the copolymer; (3) both productivity androom-temperature conductivity of the copolymer decrease with increase of the degree of sulfonation whichwas always lower than 0.5 even an excess of o-aminobenzenesulfonic acid was added, probably because thereactivity ratio of aniline (γ=2.99± 0.05) is much higher than that of o-aminobenzenesulfonic acid (γ_2 = 0.06±0.02) estimated by using Fineman-Ross method and least square method.

Effects of Water-Soluble Tomato Concentrate on Platelet Aggregation

Objective:To investigate the antiplatelet aggregation effect of water-soluble tomato concentrate(WSTC)and explore the underlying molecular mechanisms.Materials and Methods:Platelet aggregometry was used to quantify rat platelet aggregation with the maximum aggregation rate in vitro and ex vivo.Then,the fibrinogen(FIB)binding assay was employed to detect the effect of WSTC on the activation of platelet integrinαIIβ3(GP IIb/IIIa).Furthermore,Western blot was performed to assess the platelet protein levels of phosphoinositide 3-kinase 110β(PI3 K110β),protein disulfide isomerase(PDI),platelet endothelial cell adhesion molecule 1(PECAM-1),andβ1-Tubulin.Results:WSTC inhibited adenosine diphosphate(ADP)and collagen-induced platelet aggregation in a concentration-dependent manner in vitro,at IC50 values of 3.05 g/L and 8.03 g/L,respectively.Significantly reduced ex vivo ADP induced platelet aggregation was observed after oral consumption of WSTC for 4 weeks in rats;average inhibition rates were 24.42%,21.48%,and 20.87%for 25 mg/Kg,75 mg/Kg,and 150 mg/Kg WSTC,respectively.It appeared that WSTC had no influence on coagulation function in rats.Incubation with WSTC decreased FIB binding to GP IIb/IIIa by 17.47%and 32.29%at the concentrations of 0.6 and 6 g/L,respectively.WSTC at 0.6 and 6 g/L markedly downregulated PI3 K110β,PDI,and PECAM-1 in platelets,and upregulatedβ1-Tubulin,in a concentration-dependent manner.Conclusion:WSTC inhibits platelet activation through modulation of platelet skeletal stability and suppresses GP IIb/IIIa receptor-mediated platelet aggregation,likely via the PI3 K signaling pathway and PDI inhibition.