To investigate the relationship between surface currents and wave distributions in typhoons,we took the Typhoon Talim in 2017 as a case,and found that the track of the typhoon winds up to 50 m/s was almost consistent ...To investigate the relationship between surface currents and wave distributions in typhoons,we took the Typhoon Talim in 2017 as a case,and found that the track of the typhoon winds up to 50 m/s was almost consistent with the Kuroshio track,particularly from September 13 to 16,2017.The surface current data,derived from the NCEP Climate Forecast System Version 2(CFSv2)from the National Center of Atmospheric Research(NCAR),revealed that the speed of the wind-induced current exceeded that of the Kuroshio in the region with the maximum wind speed.In this study,was utilized a third-generation numeric wave model,WAVEWATCH-Ⅲ(the latest version 5.16),developed by the National Oceanic and Atmospheric Administration(NOAA),to simulate the wave fields of Typhoon Talim using the European Centre for Medium-Range Weather Forecasts(ECMWF)reanalysis wind data in 0.125°×0.125°grid as the forcing field.We found that the root-mean-square error(RMSE)of the significant wave height(SWH)was 0.34 m when validated against measurements from altimeter Jason-2.In addition,we discovered that the SWH had a similar tendency to the change in the surface current speed that was approximately 0.5 m/s at the beginning of Typhoon Talim.However,the relationship became weak as the surface current speed was below 0.2 m/s.Our findings show that the distribution of typhoon waves is resulted from the interaction of surface current and the wind-sea portion of the wave system,since the distribution pattern of wind-sea is consistent with the surface current,and there is a weak relationship between surface current and swell.展开更多
目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组...目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组成成分连翘、黄柏、金银花的有效成分和靶点;GeneCards、OMIM数据库检索“Ⅲ度烧伤”疾病相关靶点。通过生物信息学分析,包括蛋白质-蛋白质相互作用(Protein-proteininteraction,PPI)以及基因本体(Gene ontology,GO)和京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,获得了关键的有效成分、核心靶点和相关信号通路;DiscoveryStudio分子对接分析有效成分化合物与靶蛋白的结合。0.5%的DMSO溶液处理的成纤维细胞记为对照组;槲皮素(40μmol/ml)处理的成纤维细胞记为槲皮素组。采用CCK8法、Transwell实验检测细胞增殖、迁移侵袭;WB试验检测细胞p-PI3K、p-Akt蛋白。结果:共筛选出74个有效成分,331个作用靶点,AKT1为潜在的治疗靶点,木犀草素、山柰酚、槲皮素、汉黄芩素、丹皮酚为潜在的候选药物。PI3K-AKT信号通路可能在复方黄柏液治疗Ⅲ度烧伤中发挥关键作用;分子对接表明槲皮素与AKT1结合最好。与对照组相比,槲皮素组成纤维细胞增殖、迁移侵袭均显著降低,p-PI3K、p-Akt蛋白表达也显著降低(P<0.05)。结论:复方黄柏液促进Ⅲ度烧伤患者肉芽组织形成的生物活性成分为槲皮素,潜在通路为PI3K-AKT信号通路,为复方黄柏液治疗Ⅲ度烧伤的研究提供了思路。展开更多
基金Supported by the Fundamental Research Funds for Zhejiang Provincial Universities and Research Institutes(No.2019J00010)the National Key Research and Development Program of China(No.2017YFA0604901)+2 种基金the National Natural Science Foundation of China(Nos.41806005,41776183,41706206)the Public Welfare Technical Applied Research Project of Zhejiang Province of China(No.LGF19D060003)the Science and Technology Project of Zhoushan City(No.2019C21008)。
文摘To investigate the relationship between surface currents and wave distributions in typhoons,we took the Typhoon Talim in 2017 as a case,and found that the track of the typhoon winds up to 50 m/s was almost consistent with the Kuroshio track,particularly from September 13 to 16,2017.The surface current data,derived from the NCEP Climate Forecast System Version 2(CFSv2)from the National Center of Atmospheric Research(NCAR),revealed that the speed of the wind-induced current exceeded that of the Kuroshio in the region with the maximum wind speed.In this study,was utilized a third-generation numeric wave model,WAVEWATCH-Ⅲ(the latest version 5.16),developed by the National Oceanic and Atmospheric Administration(NOAA),to simulate the wave fields of Typhoon Talim using the European Centre for Medium-Range Weather Forecasts(ECMWF)reanalysis wind data in 0.125°×0.125°grid as the forcing field.We found that the root-mean-square error(RMSE)of the significant wave height(SWH)was 0.34 m when validated against measurements from altimeter Jason-2.In addition,we discovered that the SWH had a similar tendency to the change in the surface current speed that was approximately 0.5 m/s at the beginning of Typhoon Talim.However,the relationship became weak as the surface current speed was below 0.2 m/s.Our findings show that the distribution of typhoon waves is resulted from the interaction of surface current and the wind-sea portion of the wave system,since the distribution pattern of wind-sea is consistent with the surface current,and there is a weak relationship between surface current and swell.
文摘目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组成成分连翘、黄柏、金银花的有效成分和靶点;GeneCards、OMIM数据库检索“Ⅲ度烧伤”疾病相关靶点。通过生物信息学分析,包括蛋白质-蛋白质相互作用(Protein-proteininteraction,PPI)以及基因本体(Gene ontology,GO)和京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,获得了关键的有效成分、核心靶点和相关信号通路;DiscoveryStudio分子对接分析有效成分化合物与靶蛋白的结合。0.5%的DMSO溶液处理的成纤维细胞记为对照组;槲皮素(40μmol/ml)处理的成纤维细胞记为槲皮素组。采用CCK8法、Transwell实验检测细胞增殖、迁移侵袭;WB试验检测细胞p-PI3K、p-Akt蛋白。结果:共筛选出74个有效成分,331个作用靶点,AKT1为潜在的治疗靶点,木犀草素、山柰酚、槲皮素、汉黄芩素、丹皮酚为潜在的候选药物。PI3K-AKT信号通路可能在复方黄柏液治疗Ⅲ度烧伤中发挥关键作用;分子对接表明槲皮素与AKT1结合最好。与对照组相比,槲皮素组成纤维细胞增殖、迁移侵袭均显著降低,p-PI3K、p-Akt蛋白表达也显著降低(P<0.05)。结论:复方黄柏液促进Ⅲ度烧伤患者肉芽组织形成的生物活性成分为槲皮素,潜在通路为PI3K-AKT信号通路,为复方黄柏液治疗Ⅲ度烧伤的研究提供了思路。