Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan (South Punjab, Balochistan and Khyber Pakhtunkhwa) comprised mostly PermoTriassic to Recent sediments with subordinate igneous and metamorphic rocks....Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan (South Punjab, Balochistan and Khyber Pakhtunkhwa) comprised mostly PermoTriassic to Recent sediments with subordinate igneous and metamorphic rocks. These sedimentary strata folded and faulted by geodynamic and tectonic forces occurred during Late Cretaceous to Recent revealed through anticlinal and synclinal foldings and active faultings. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan areas host many economic minerals like copper and chromite, construction stones, marble limestones, gypsum and cement resources, uranium and other radioactive mineral resources, low-quality iron, phosphate and muddy coal, high-quality gemstones, petroleum potential and excellent water resources and many other rocks and minerals. High-quality window and faden quartz crystals deposited in fractures and fissures as vugs and veins deposited by high-temperature hypothermal solution created by the tectonic compression process. The area has economic chromite and magnesite deposits. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan areas have large cement raw materials/resources (limestones, gypsum and shale/clays available mostly on same sites) which vitally need to install many cement industries in these areas because the ideal central location and now only one cement industry (Dera Ghazi Khan cement industry) is in operation. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan consists of excellent surface water resources which need the construction of smaller and medium-sized dams on different rud kohi/streams/rivers for the development of the area. Sakhi Sarwar area of Dera Ghazi Khan (South Punjab) yielded fossil of new Tomistominae (False Gharial) Gavialidae and further its surroundings recently yielded fossils of famous vertebrates like reptiles (dinosaurs, crocodiles, pterosaur and snake), birds and mammals, and tracks/trackways of Late Cretaceous archosaurs like Sauropaonia, Ornithopaonia and Pteropaonia.展开更多
Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected pati...Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.展开更多
文摘Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan (South Punjab, Balochistan and Khyber Pakhtunkhwa) comprised mostly PermoTriassic to Recent sediments with subordinate igneous and metamorphic rocks. These sedimentary strata folded and faulted by geodynamic and tectonic forces occurred during Late Cretaceous to Recent revealed through anticlinal and synclinal foldings and active faultings. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan areas host many economic minerals like copper and chromite, construction stones, marble limestones, gypsum and cement resources, uranium and other radioactive mineral resources, low-quality iron, phosphate and muddy coal, high-quality gemstones, petroleum potential and excellent water resources and many other rocks and minerals. High-quality window and faden quartz crystals deposited in fractures and fissures as vugs and veins deposited by high-temperature hypothermal solution created by the tectonic compression process. The area has economic chromite and magnesite deposits. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan areas have large cement raw materials/resources (limestones, gypsum and shale/clays available mostly on same sites) which vitally need to install many cement industries in these areas because the ideal central location and now only one cement industry (Dera Ghazi Khan cement industry) is in operation. The Northern Koh Sulaiman foldbelt, Shirani, North and South Waziristan consists of excellent surface water resources which need the construction of smaller and medium-sized dams on different rud kohi/streams/rivers for the development of the area. Sakhi Sarwar area of Dera Ghazi Khan (South Punjab) yielded fossil of new Tomistominae (False Gharial) Gavialidae and further its surroundings recently yielded fossils of famous vertebrates like reptiles (dinosaurs, crocodiles, pterosaur and snake), birds and mammals, and tracks/trackways of Late Cretaceous archosaurs like Sauropaonia, Ornithopaonia and Pteropaonia.
基金project entitled PCR and RFLP analysis of Leishmania species from suspected cutaneous leishmaniasis patients and animal reservoirs in Waziristan funded by Relief International,Pakistan(DOSFY15)
文摘Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.
