Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model...Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated.The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR.The serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western blot.Results:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model.WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD model.WZR also significantly reduced the expression of pPLC/PLC,pPKC/PKC and CX43.Targeting the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions.Conclusion:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.展开更多
Objective:To treat patients with menstrual disorders with Wenjing Decoction and analyze its therapeutic effects.Metlods:80 patients with menstrual disorders were selected as the research subjects,and they were divided...Objective:To treat patients with menstrual disorders with Wenjing Decoction and analyze its therapeutic effects.Metlods:80 patients with menstrual disorders were selected as the research subjects,and they were divided into two groups(experimental group and control group),with each group given different treatment methods.The control group was given conventional Western medical treatment while the experimental group was given Wenjing Decoction treatment,and the effects of different treatment methods on the patients were compared.Results:The total number of effectively treated patients in the experimental group(39 cases,97.50%)was more than the that of the control group(25 cases,62.50%)。and the quality of life score of the experimental group(91.56±4.44 points)was higher that of the control group(81.72±3.19 points),and the number of menstnual recovery cycles(1.11±0.41)was less than that of the control group patients(2.42±1.25 times),P<0.05.Conclusion:The application of Wenjing Decoction for patients with menstrual disorders can significantly improve the treatment efficacy and is beneficial to improve the quality of life of the patients and shorten the number of cycles that menstruation starts to return to normal.展开更多
OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJ...OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJD) for the treatment of CCBS.METHODS: CCBS was induced in rats with an icewater bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells(VECs) and vascular smooth muscle cells(VSMCs) were evaluated.RESULTS: Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues.CONCLUSION: Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.展开更多
THE day for the interviewhappened to be the final trainingday for the girl gymnasts of theNational Gymnastics Team, who were togive a performance in Taiwan. It was already six o’clock in theafternoon, and head coach ...THE day for the interviewhappened to be the final trainingday for the girl gymnasts of theNational Gymnastics Team, who were togive a performance in Taiwan. It was already six o’clock in theafternoon, and head coach Huang Yubindid not show any sign of ending thetraining session. When Bi Wenjingspotted the reporter, she tapped herstomach, indicating that she felt hungry.展开更多
According to Zhongjing Zhang's academic thought,gynecological diseases mostly result from deficiency,accumulated cold,and qi stagnation,so the treatment of gynecological miscellaneous diseases should take qi and b...According to Zhongjing Zhang's academic thought,gynecological diseases mostly result from deficiency,accumulated cold,and qi stagnation,so the treatment of gynecological miscellaneous diseases should take qi and blood as the general principle,and focus on the liver.The pathogenesis of mammary glands hyperplasia(MGH)is liver and kidney deficiency,disharmony of the thoroughfare vessel and the conception vessel,the causes of which are emotional imbalance,liver qi depression,and failure of transportation due to spleen deficiency.The superficial causes include qi stagnation,phlegm coagulation,and blood stasis,which are mainly related to the liver,spleen,kidney,the thoroughfare vessel,and the conception vessel.In clinical treatment of MGH,Xiaochaihu Decoction or Sini Powder can be used for qi stagnation syndrome,Xiao Xianxiong Decoction for phlegm coagulation syndrome,Guizhi Fuling Pill or Dahuang Zhechong Pill for spleen deficiency syndrome,and Wenjing Decoction for the deficiency-cold syndrome.展开更多
Objective: To explore the therapeutic mechanism of Shuguan Granule (舒关冲剂, SGG) in treating mid late stage rheumatoid arthritis (RA). Methods: Based on the principle of reinforcing Kidney to treat arthritis, removi...Objective: To explore the therapeutic mechanism of Shuguan Granule (舒关冲剂, SGG) in treating mid late stage rheumatoid arthritis (RA). Methods: Based on the principle of reinforcing Kidney to treat arthritis, removing phlegm to relieve stasis, two SGG, Shuguan Wenjing (SW) and Shuguan Qingluo (SQ) were prepared and used to treat 44 and 43 mid late stage patients of RA respectively. The clinical results were compared with those of Wangbi Granule. Animal experiments on the effect of these two SGG were conducted. Results: The total effective rate of the SW, SQ and control group was 88.64%, 93.02% and 73.17% respectively. Results of experimental studies in rats with adjuvant induced arthritis showed that the two SGG obviously raise the level of serum superoxide dismutase, and lowered the levels of serum interleukin 1, plasma prostaglandin E 2 and thromboxane B 2, as compared with the model group, the difference was significant ( P <0.05, P <0.01). Conclusion: The two SGG have a comprehensive function of anti inflammatory, analgesic, antioxidatory, antihypercoagulatory and immunoregulatory effect.展开更多
基金supported by Beijing University of Chinese Medicine(1000061224003)National Natural Science Foundation of China(81903766,81900603,82104440)。
文摘Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated.The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR.The serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western blot.Results:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model.WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD model.WZR also significantly reduced the expression of pPLC/PLC,pPKC/PKC and CX43.Targeting the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions.Conclusion:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.
文摘Objective:To treat patients with menstrual disorders with Wenjing Decoction and analyze its therapeutic effects.Metlods:80 patients with menstrual disorders were selected as the research subjects,and they were divided into two groups(experimental group and control group),with each group given different treatment methods.The control group was given conventional Western medical treatment while the experimental group was given Wenjing Decoction treatment,and the effects of different treatment methods on the patients were compared.Results:The total number of effectively treated patients in the experimental group(39 cases,97.50%)was more than the that of the control group(25 cases,62.50%)。and the quality of life score of the experimental group(91.56±4.44 points)was higher that of the control group(81.72±3.19 points),and the number of menstnual recovery cycles(1.11±0.41)was less than that of the control group patients(2.42±1.25 times),P<0.05.Conclusion:The application of Wenjing Decoction for patients with menstrual disorders can significantly improve the treatment efficacy and is beneficial to improve the quality of life of the patients and shorten the number of cycles that menstruation starts to return to normal.
基金Supported by Natural Science Foundation-funded Project Based on the Theory of"Cold Leads to Blood Vessel Collection and Blood Stasis",to Discuss the Characteristics of Microvascular Endothelial Function Changes in the Syndrome of Cold Coagulation and Blood Stasis,and the Influence of TCM(No.81373514)。
文摘OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJD) for the treatment of CCBS.METHODS: CCBS was induced in rats with an icewater bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells(VECs) and vascular smooth muscle cells(VSMCs) were evaluated.RESULTS: Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues.CONCLUSION: Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.
文摘THE day for the interviewhappened to be the final trainingday for the girl gymnasts of theNational Gymnastics Team, who were togive a performance in Taiwan. It was already six o’clock in theafternoon, and head coach Huang Yubindid not show any sign of ending thetraining session. When Bi Wenjingspotted the reporter, she tapped herstomach, indicating that she felt hungry.
基金special project of Traditional Chinese Medicine Research in Henan Province(20-21ZY1008).
文摘According to Zhongjing Zhang's academic thought,gynecological diseases mostly result from deficiency,accumulated cold,and qi stagnation,so the treatment of gynecological miscellaneous diseases should take qi and blood as the general principle,and focus on the liver.The pathogenesis of mammary glands hyperplasia(MGH)is liver and kidney deficiency,disharmony of the thoroughfare vessel and the conception vessel,the causes of which are emotional imbalance,liver qi depression,and failure of transportation due to spleen deficiency.The superficial causes include qi stagnation,phlegm coagulation,and blood stasis,which are mainly related to the liver,spleen,kidney,the thoroughfare vessel,and the conception vessel.In clinical treatment of MGH,Xiaochaihu Decoction or Sini Powder can be used for qi stagnation syndrome,Xiao Xianxiong Decoction for phlegm coagulation syndrome,Guizhi Fuling Pill or Dahuang Zhechong Pill for spleen deficiency syndrome,and Wenjing Decoction for the deficiency-cold syndrome.
文摘Objective: To explore the therapeutic mechanism of Shuguan Granule (舒关冲剂, SGG) in treating mid late stage rheumatoid arthritis (RA). Methods: Based on the principle of reinforcing Kidney to treat arthritis, removing phlegm to relieve stasis, two SGG, Shuguan Wenjing (SW) and Shuguan Qingluo (SQ) were prepared and used to treat 44 and 43 mid late stage patients of RA respectively. The clinical results were compared with those of Wangbi Granule. Animal experiments on the effect of these two SGG were conducted. Results: The total effective rate of the SW, SQ and control group was 88.64%, 93.02% and 73.17% respectively. Results of experimental studies in rats with adjuvant induced arthritis showed that the two SGG obviously raise the level of serum superoxide dismutase, and lowered the levels of serum interleukin 1, plasma prostaglandin E 2 and thromboxane B 2, as compared with the model group, the difference was significant ( P <0.05, P <0.01). Conclusion: The two SGG have a comprehensive function of anti inflammatory, analgesic, antioxidatory, antihypercoagulatory and immunoregulatory effect.
